Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 279,645 +A 2.1% intergenic (‑47/‑33) RCAP_rcc00242 ← / → dapD hypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034279,6450.→A 2.1% 23.2 ‑0.3 ‑0.0382intergenic (‑47/‑33)RCAP_rcc00242/dapDhypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (197/177):  total (202/180)
Fisher's exact test strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 419 total aligned reads displayed.

TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742
                                                                                                                                                                                                      
ttttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAc                                                                                                   >  1:3546852/1‑101 (MQ=255)
ttttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAc                                                                                                   >  1:5971758/1‑101 (MQ=255)
ttttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAc                                                                                                   <  1:2349307/101‑1 (MQ=255)
ttttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAc                                                                                                   <  1:3754526/101‑1 (MQ=255)
 tttGCATCCATGTTGCTGTCACGGAACGGGCGGTAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcc                                                                                                  <  1:7020224/101‑1 (MQ=255)
 tttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcc                                                                                                  <  1:4429082/101‑1 (MQ=255)
  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcca                                                                                                 <  1:6722636/101‑2 (MQ=255)
  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcca                                                                                                 >  1:4245025/1‑100 (MQ=255)
  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcca                                                                                                 >  1:5742765/1‑100 (MQ=255)
   tGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccat                                                                                                >  2:1258451/1‑99 (MQ=255)
   tGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccat                                                                                                <  1:456483/101‑3 (MQ=255)
    gCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccatg                                                                                               <  2:4244830/101‑4 (MQ=255)
      aTCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGaa                                                                                            >  2:1880412/1‑101 (MQ=255)
      aTCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGaa                                                                                            <  1:1564521/101‑1 (MQ=255)
        ccATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATg                                                                                          >  2:2737575/1‑101 (MQ=255)
        ccATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATg                                                                                          >  1:276980/1‑101 (MQ=255)
          atgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGcc                                                                                        <  2:5005107/101‑1 (MQ=255)
           tgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGcga                                                                                       >  2:805653/1‑99 (MQ=255)
           tgatgATGTCACGGAACGGGTTGGCACGGCTTGCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCa                                                                                       <  2:279886/101‑1 (MQ=255)
            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      <  1:3457490/101‑1 (MQ=255)
             atgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACt                                                                                     >  1:6243532/1‑101 (MQ=255)
             atgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACt                                                                                     >  2:750646/1‑101 (MQ=255)
             atgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACt                                                                                     <  2:4901635/101‑1 (MQ=255)
               tcTGTCACGGAACCGGTGGGAACGTCTCTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   <  1:6928731/100‑1 (MQ=255)
               gCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   >  2:593763/1‑101 (MQ=255)
               gCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   <  1:1026762/101‑1 (MQ=255)
                cTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGa                                                                                  >  1:1072960/1‑101 (MQ=255)
                cTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGa                                                                                  <  1:5348892/101‑1 (MQ=255)
                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 <  1:6347319/101‑1 (MQ=255)
                  gTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCACAACTATAGGGGGAAAA.CCATGAATGCCACTTGaaa                                                                                >  1:6301922/1‑101 (MQ=255)
                    cACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAaga                                                                              <  2:5802431/101‑1 (MQ=255)
                    cACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAaga                                                                              <  2:4441066/101‑1 (MQ=255)
                      cGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAgg                                                                            >  2:932912/1‑101 (MQ=255)
                       ggAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGa                                                                           >  2:1578355/1‑101 (MQ=255)
                       ggAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGa                                                                           >  2:5565338/1‑101 (MQ=255)
                       ggAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGa                                                                           >  1:3903825/1‑101 (MQ=255)
                        gAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAc                                                                          <  2:2873456/101‑1 (MQ=255)
                         aaCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAcc                                                                         <  2:5992042/101‑1 (MQ=255)
                          aCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCg                                                                        >  1:3122775/1‑101 (MQ=255)
                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  1:4672779/1‑101 (MQ=255)
                              gTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTcc                                                                    <  1:4005745/101‑1 (MQ=255)
                              gTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTcc                                                                    <  2:4359650/101‑1 (MQ=255)
                                gggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGa                                                                  >  1:820007/1‑101 (MQ=255)
                                 ggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGAt                                                                 <  1:3821370/101‑1 (MQ=255)
                                  gAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATg                                                                <  1:2465705/101‑1 (MQ=255)
                                   aaCGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGt                                                               <  1:5291985/101‑1 (MQ=255)
                                      ggCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            >  2:4095638/1‑101 (MQ=255)
                                      ggCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            >  2:5186335/1‑101 (MQ=255)
                                         tttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACg                                                         >  2:2100551/1‑101 (MQ=255)
                                          ttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGc                                                        <  2:6964692/101‑1 (MQ=255)
                                            cGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCa                                                      >  1:4949525/1‑101 (MQ=255)
                                             gTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGTACCGTTCCGATGTCGAACGCCAt                                                     >  2:7110157/1‑101 (MQ=255)
                                             gTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCAt                                                     >  1:1253372/1‑101 (MQ=255)
                                             gTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGCTCCGATGTCGAACGCCAt                                                     >  2:665446/1‑101 (MQ=255)
                                              tCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATg                                                    <  2:654624/101‑1 (MQ=255)
                                                tttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCt                                                  <  2:2382220/101‑1 (MQ=255)
                                                tttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCt                                                  <  2:6094305/101‑1 (MQ=255)
                                                tttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCt                                                  >  1:764737/1‑101 (MQ=255)
                                                   cATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGCAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGa                                               >  1:3318911/1‑101 (MQ=255)
                                                   cATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGa                                               >  1:6800030/1‑101 (MQ=255)
                                                   cATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGa                                               >  2:6896553/1‑101 (MQ=255)
                                                    atatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGaa                                              >  1:2026213/1‑101 (MQ=255)
                                                     tatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAg                                             <  2:6799676/101‑1 (MQ=255)
                                                     tatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAg                                             <  1:408584/101‑1 (MQ=255)
                                                      atCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGc                                            >  2:5848038/1‑101 (MQ=255)
                                                      atCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGc                                            >  2:3474551/1‑101 (MQ=255)
                                                       tCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGGCGAACGCCATGCTCGAAGCt                                           >  2:1029125/1‑101 (MQ=255)
                                                          tGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGcc                                        <  2:2665552/101‑1 (MQ=255)
                                                          tGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGcc                                        <  1:5854883/101‑1 (MQ=255)
                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    <  2:3122662/101‑1 (MQ=255)
                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    >  2:5398913/1‑101 (MQ=255)
                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    >  1:1690059/1‑101 (MQ=255)
                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    >  1:5181070/1‑101 (MQ=255)
                                                                 tGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAg                                 >  2:5977749/1‑101 (MQ=255)
                                                                  gcgcGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgc                                >  2:6486015/1‑101 (MQ=255)
                                                                  gcgcGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgc                                <  1:1000311/101‑1 (MQ=255)
                                                                  gcgcGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgc                                <  1:1888936/101‑1 (MQ=255)
                                                                  gcgcGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgc                                <  1:2118252/101‑1 (MQ=255)
                                                                  gcgcGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgc                                <  1:2254565/101‑1 (MQ=255)
                                                                     cgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccgc                             <  1:923745/101‑1 (MQ=255)
                                                                       aGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCt                           >  1:2923905/1‑101 (MQ=255)
                                                                         gggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGATGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTgg                         >  1:2636038/1‑101 (MQ=255)
                                                                         gggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTgg                         <  2:6243213/101‑1 (MQ=255)
                                                                           gCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGa                       <  2:260472/101‑1 (MQ=255)
                                                                            cTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAg                      <  1:2609833/101‑1 (MQ=255)
                                                                                ccccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTc                  <  2:1108823/101‑1 (MQ=255)
                                                                                cccaAACTATAAGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGTCGCCTGGGAgggct                  >  2:334366/1‑98 (MQ=255)
                                                                                    aaCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTACGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGa              >  2:4864229/1‑101 (MQ=255)
                                                                                      cTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGaca            <  2:6392547/101‑1 (MQ=255)
                                                                                      cTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGaaa            >  1:4759414/1‑99 (MQ=255)
                                                                                       tataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGacac           <  1:5565628/101‑1 (MQ=255)
                                                                                        ataGGGGGAAAA.CCATGAATGTCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAcc          <  2:5729024/101‑1 (MQ=255)
                                                                                          aGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCt        <  1:4344757/101‑1 (MQ=255)
                                                                                          aGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCt        >  2:3991367/1‑101 (MQ=255)
                                                                                          aGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCt        >  2:4697643/1‑101 (MQ=255)
                                                                                           gggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGCCACCCTc       >  2:3600890/1‑101 (MQ=255)
                                                                                           gggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTc       <  1:3018517/101‑1 (MQ=255)
                                                                                           gggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTc       <  1:2710921/101‑1 (MQ=255)
                                                                                             gggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCAc     <  2:3201555/101‑1 (MQ=255)
                                                                                                 aaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGcc  >  2:417226/1‑101 (MQ=255)
                                                                                                                                                                                                      
TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG