Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 296,280 +T 5.3% intergenic (‑80/‑203) RCAP_rcc00258 ← / → RCAP_rcc00259 lipoprotein/porin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034296,2800.→T 5.3% 54.7 ‑3.5 ‑0.1347intergenic (‑80/‑203)RCAP_rcc00258/RCAP_rcc00259lipoprotein/porin family protein
Reads supporting (aligned to +/- strand):  new base (5/10):  ref base (205/123):  total (211/136)
Fisher's exact test strand distribution p-value = 3.01e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.92e-01

Only 100 of 391 total aligned reads displayed.

CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCG  >  NC_014034/296180‑296379
                                                                                                                                                                                                        
cAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTc                                                                                                     <  2:3428136/101‑1 (MQ=255)
cAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTc                                                                                                     >  1:1525864/1‑101 (MQ=255)
  aaGAGGTTGCTTTCTTCATCGGATCGACTGCCCCCGCGGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTccc                                                                                                   <  1:2182914/101‑1 (MQ=255)
   agagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccc                                                                                                  <  1:2380735/101‑1 (MQ=255)
    gagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTccccc                                                                                                 <  2:122248/101‑1 (MQ=255)
      ggTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgc                                                                                               <  2:3505749/101‑3 (MQ=255)
      ggTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgc                                                                                               <  1:1933410/101‑3 (MQ=255)
          gCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCgggc                                                                                          >  1:402948/1‑100 (MQ=255)
          gCTTGCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGa                                                                                          <  2:2431547/101‑1 (MQ=255)
           cTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAc                                                                                         >  2:174086/1‑101 (MQ=255)
           cTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAc                                                                                         <  2:4309685/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                        <  1:5134784/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                        <  1:3193030/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                        >  2:3771032/1‑101 (MQ=255)
             ttcttcATACGATCGACAGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccg                                                                                       >  1:4236839/1‑101 (MQ=255)
              tcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgc                                                                                      <  2:4538643/101‑1 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                     <  1:5321098/101‑1 (MQ=255)
               cctcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                     <  1:2460941/99‑1 (MQ=255)
                ttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgccc                                                                                    >  2:5622582/1‑101 (MQ=255)
                ttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgccc                                                                                    >  1:664756/1‑101 (MQ=255)
                ttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATATTT.CCCCCGCGGGAccgccc                                                                                    >  1:4367627/1‑101 (MQ=255)
                 tcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccg                                                                                   >  2:1666396/1‑101 (MQ=255)
                 tcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccg                                                                                   <  2:5054986/101‑1 (MQ=255)
                 tcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccg                                                                                   >  1:396299/1‑101 (MQ=255)
                 atctACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccg                                                                                   <  2:3662654/98‑1 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                  >  1:539505/1‑101 (MQ=255)
                    tACGACCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcgg                                                                                <  1:2280904/101‑1 (MQ=255)
                     aCGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgagat                                                                               >  1:3662803/1‑97 (MQ=255)
                     aCGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcggc                                                                               >  2:1835740/1‑101 (MQ=255)
                      cGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgcggcg                                                                              >  1:1293877/1‑101 (MQ=255)
                       gATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGcggcgg                                                                             >  1:6111834/1‑101 (MQ=255)
                         tCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCgggg                                                                           >  1:4475464/1‑101 (MQ=255)
                          cGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgc                                                                          >  2:6629114/1‑101 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcg                                                                         >  2:7038229/1‑101 (MQ=255)
                            aCTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcgc                                                                        >  1:1788875/1‑101 (MQ=255)
                            aCTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcgc                                                                        >  1:75572/1‑101 (MQ=255)
                             cTGGCCCCAGAGGCTGAACCCATTGGATTTTCCTATCTGTAGACGATGGGCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGAGGGGCGCa                                                                       <  2:807466/101‑1 (MQ=255)
                              tGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCaa                                                                      >  1:3530987/1‑101 (MQ=255)
                                   ccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCCcc                                                                 >  2:4715867/1‑101 (MQ=255)
                                     gCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCAACGGCGGGGCGCCACCTcccc                                                               >  2:4191834/1‑100 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             <  2:5846137/101‑5 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             >  1:2506714/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             <  2:519358/101‑5 (MQ=255)
                                        ggCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCgg                                                           >  2:6364829/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                        >  1:3715440/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                        >  1:2826312/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                        >  2:1391647/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                        >  1:1129783/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACC.CCCGCCGGTcc                                                        >  1:4881525/1‑101 (MQ=255)
                                            gAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccc                                                       <  2:922730/101‑1 (MQ=255)
                                            gAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccc                                                       >  1:6758683/1‑101 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      <  2:2674729/101‑2 (MQ=255)
                                               ctcATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccagc                                                    <  1:6449408/99‑4 (MQ=255)
                                                ccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccagcg                                                   >  2:3625515/1‑97 (MQ=255)
                                                 cATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGCCAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGGGCAACC.CCCGCCGGTcccaccgg                                                  >  2:5267668/1‑96 (MQ=255)
                                                   ttGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCgggg                                               >  2:4763347/1‑101 (MQ=255)
                                                   ttGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCgggg                                               >  2:669814/1‑101 (MQ=255)
                                                    tGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                              <  1:6921889/101‑1 (MQ=255)
                                                    tGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                              >  1:4661764/1‑101 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                             >  2:2749150/1‑101 (MQ=255)
                                                     ggCTATTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGGCGAACCCTCCGCCGGTcccgggggggg                                               >  1:926665/1‑93 (MQ=255)
                                                      gCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGAc                                            <  2:2937968/101‑1 (MQ=255)
                                                       tttttCCTGCCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCGGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACt                                           <  1:309442/100‑1 (MQ=255)
                                                        ttttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtg                                          >  1:553255/1‑101 (MQ=255)
                                                              tGTCTCTGGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaa                                    <  1:3285718/101‑1 (MQ=255)
                                                              tGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaa                                    <  1:7038603/101‑1 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                   >  1:5688715/1‑101 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                   <  2:1962034/101‑1 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                   <  1:4559739/101‑1 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                   >  2:6059900/1‑101 (MQ=255)
                                                                ttgctcgCCGATGGCGGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCGGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaatg                                  <  1:5261476/95‑3 (MQ=255)
                                                                 ctctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGc                                 >  2:4027530/1‑101 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGTAACCT.CCGCCGGT.CCCAGCGGGGGACTGAGGCAAAGCTg                               >  2:3182561/1‑101 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTg                               >  1:6042093/1‑101 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTg                               >  2:113704/1‑101 (MQ=255)
                                                                    tAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACC.CCCGCCGGT.CCCAGCGGGGGACTGTGGCACACCTGc                              >  2:6201332/1‑101 (MQ=255)
                                                                     aGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGca                             >  1:169539/1‑101 (MQ=255)
                                                                      gCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcac                            <  1:4735434/101‑1 (MQ=255)
                                                                        cggtGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          <  2:4921808/98‑1 (MQ=255)
                                                                        cGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          <  1:6591983/101‑1 (MQ=255)
                                                                        cGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          >  2:1070663/1‑101 (MQ=255)
                                                                         gATGGCCGGGGGGTCGACAAGA.TTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                         <  2:2206976/101‑1 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                         >  1:1032552/1‑101 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                         >  1:720851/1‑101 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                         >  2:4423664/1‑101 (MQ=255)
                                                                           tGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCAcgc                       <  2:3905171/101‑1 (MQ=255)
                                                                            ggCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCAcgcg                      <  1:2727029/101‑1 (MQ=255)
                                                                               cGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGtt                   <  2:480044/101‑1 (MQ=255)
                                                                                 ggggggCGACCAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTc                 <  1:6288079/101‑1 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                <  2:6807713/101‑1 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt            <  2:6113735/101‑1 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt            <  2:6507615/101‑1 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt            >  2:5403860/1‑101 (MQ=255)
                                                                                       cGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTg           >  1:4950946/1‑101 (MQ=255)
                                                                                         aCAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGc         >  2:5151222/1‑101 (MQ=255)
                                                                                         aCAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGc         >  2:1595054/1‑101 (MQ=255)
                                                                                          cAAGACTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGcc        <  2:232526/101‑1 (MQ=255)
                                                                                           aaGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg       <  2:73114/101‑1 (MQ=255)
                                                                                           aaGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg       <  1:1387944/101‑1 (MQ=255)
                                                                                            aagactttCCCCCCGCGGCACCGCCCGCGGCGGGGCGTATCC.GCCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg       <  2:5432044/96‑1 (MQ=255)
                                                                                                                                                                                                        
CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCG  >  NC_014034/296180‑296379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG