Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,171 +C 100% coding (1314/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1711.→C100.0% 844.2 226coding (1314/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (120/106):  ref base (0/0):  total (120/106)

Only 100 of 257 total aligned reads displayed.

GGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.G.CCGAGG.GCA.G.TGGAA.CCGCGATGG.AA.GGC.T.GCCGAG.TGTTCGCCCGGCAGGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGC  >  NC_014034/3256072‑3256256
                                                                                                      |                                                                                               
ggtggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAgg                                                                                                  <  2:7056327/98‑1 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcg                                                                                                <  1:2843451/101‑3 (MQ=255)
    gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcgca                                                                                              >  1:4656964/1‑97 (MQ=255)
    gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcgca                                                                                              >  1:4201002/1‑97 (MQ=255)
    gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcgca                                                                                              <  2:4509198/101‑5 (MQ=255)
      cGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcgcagc                                                                                            <  2:2943674/101‑7 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcgcagct                                                                                           >  2:3233967/1‑94 (MQ=255)
       gATCTGGGCGCGGCCGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcgcagct                                                                                           <  1:5715157/101‑8 (MQ=255)
        aTCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcgcagctg                                                                                          >  2:1003029/1‑93 (MQ=255)
         tCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAggcgcagctgg                                                                                         <  1:5203964/101‑10 (MQ=255)
            gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAA.c                                                                                    >  2:1534948/1‑101 (MQ=255)
            gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAA.c                                                                                    >  2:2497424/1‑101 (MQ=255)
             ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAA.cc                                                                                   <  2:5685889/101‑1 (MQ=255)
                gctgcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAA.cccgc                                                                                <  2:5725431/98‑4 (MQ=255)
                 cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAA.cccgcg                                                                               <  2:790076/101‑5 (MQ=255)
                  ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAA.cccgcga                                                                              >  1:6862977/1‑96 (MQ=255)
                     ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATgg                                                                            >  2:1688053/1‑101 (MQ=255)
                      gTGCAGCGCGTCGCGGCCGGCGGGGTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggc                                                                           <  2:5815899/101‑2 (MQ=255)
                      gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggc                                                                           >  1:1659836/1‑100 (MQ=255)
                       tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggca                                                                          <  1:839814/101‑3 (MQ=255)
                        gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcac                                                                         <  1:6203696/101‑4 (MQ=255)
                         cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacg                                                                        <  2:3032995/101‑5 (MQ=255)
                          aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacgg                                                                       >  1:4539176/1‑96 (MQ=255)
                            cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacggct                                                                     <  2:3191098/101‑8 (MQ=255)
                            cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacggct                                                                     >  2:4453042/1‑94 (MQ=255)
                            cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacggct                                                                     <  2:4840956/101‑8 (MQ=255)
                             gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacggctg                                                                    <  1:3378238/101‑9 (MQ=255)
                             gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacggctg                                                                    >  1:2381646/1‑93 (MQ=255)
                              cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacggctgg                                                                   >  2:1775963/1‑92 (MQ=255)
                              cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATggcacggctgg                                                                   <  1:6447483/101‑10 (MQ=255)
                                    ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAg.t                                                          <  2:4075793/101‑1 (MQ=255)
                                      ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGC.TGGCCGAg.ttg                                                        <  1:415225/101‑3 (MQ=255)
                                       caccgggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAg.ttgc                                                       >  2:3559436/5‑98 (MQ=255)
                                        gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAg.ttgct                                                      >  2:922705/1‑97 (MQ=255)
                                         aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAg.ttgctc                                                     >  2:6639808/1‑96 (MQ=255)
                                            ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAg.ttgctcgcc                                                  >  2:4307736/1‑93 (MQ=255)
                                            ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAg.ttgctcgcc                                                  >  2:4743530/1‑93 (MQ=255)
                                            ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAg.ttgctcgcc                                                  >  2:5490081/1‑93 (MQ=255)
                                               ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGccccg                                                >  2:1194063/1‑99 (MQ=255)
                                               ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCgg                                                >  2:2208552/1‑101 (MQ=255)
                                               ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGC.TGGCCGAGTTGCTCGCCCgg                                                <  1:5530528/101‑1 (MQ=255)
                                                 ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGcc                                              >  1:2707928/1‑100 (MQ=255)
                                                 ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCa                                              <  1:1732741/101‑1 (MQ=255)
                                                  tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGTAC.GGC.TGGCCGAGTTGCTCGCCCGGCAg                                             <  1:5056162/101‑1 (MQ=255)
                                                  tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGC.TGGCCGAGTTGCTCGCCCGGCAg                                             <  2:1387151/101‑1 (MQ=255)
                                                   gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGG.CACGGC.TGGCCGAGTTGCTCGCCCGGCAgg                                            <  1:1306687/101‑1 (MQ=255)
                                                    cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAggg                                           <  2:83071/101‑1 (MQ=255)
                                                    cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAggg                                           >  2:4165839/1‑101 (MQ=255)
                                                    cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAggg                                           >  2:620040/1‑101 (MQ=255)
                                                     tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCCgggg                                          >  2:3332036/1‑100 (MQ=255)
                                                     tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAcggg                                          >  2:1811227/1‑100 (MQ=255)
                                                     tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGgc                                          <  1:5873863/101‑1 (MQ=255)
                                                      cAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGgcg                                         >  1:7095951/1‑101 (MQ=255)
                                                        aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCCGCAGGGggc.g                                      >  1:3757777/1‑101 (MQ=255)
                                                          ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGgggccgg                                     >  1:1439007/1‑98 (MQ=255)
                                                            cggcggGGACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCCGCAGGGCGC.gggga                                  >  2:6238865/1‑98 (MQ=255)
                                                            cggcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCCC.gggca                                  >  1:513910/1‑98 (MQ=255)
                                                            cggcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGA.TGGCCGAGTTGCTCGCCCGGCAGGGCGC.gggta                                  <  1:6255886/101‑4 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCCGGGGGC.ggggac                                 >  2:4429733/1‑97 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGC.gggcac                                 <  2:5279246/101‑5 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGC.gggcac                                 <  2:1659775/101‑5 (MQ=255)
                                                              gcggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGC.gggcacg                                <  1:3832559/101‑6 (MQ=255)
                                                               cggGCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCAc.gg                               <  1:5776530/101‑1 (MQ=255)
                                                                gggCACCGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCAc.ggg                              >  2:2654077/1‑100 (MQ=255)
                                                                 cgatctgCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCAc.gggc                             <  1:7075943/95‑3 (MQ=255)
                                                                     ccGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCCGCAGGGCGCGGGCACGGGcggcc                          >  2:5419697/1‑101 (MQ=255)
                                                                      ctcATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggccc                         <  2:4392502/99‑1 (MQ=255)
                                                                      cGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggccc                         >  1:884256/1‑101 (MQ=255)
                                                                      cGCATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggccc                         <  2:206261/101‑1 (MQ=255)
                                                                        cATGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccgg                       <  1:1535008/101‑1 (MQ=255)
                                                                         aTGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggc                      >  2:365933/1‑101 (MQ=255)
                                                                         aTGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggccccgc                      >  2:6789965/1‑101 (MQ=255)
                                                                          tGGCCGCGGGTCTGACCCTGG.CCGAGGCGCATC.TGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggcc                     <  1:6330146/101‑1 (MQ=255)
                                                                          tGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggcc                     <  1:1027345/101‑1 (MQ=255)
                                                                          tGGCCGCGGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggcc                     <  2:6387128/101‑1 (MQ=255)
                                                                          tGGCCGCGGGTCTGACCCT.GTCCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggcc                     <  1:5609008/101‑1 (MQ=255)
                                                                               gcgGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGCTT.GCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCt                <  2:4962909/101‑1 (MQ=255)
                                                                               gcgGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCt                <  2:2302195/101‑1 (MQ=255)
                                                                                cgGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCggcctg               >  1:6627288/1‑96 (MQ=255)
                                                                                cgGGTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTg               >  1:4470165/1‑101 (MQ=255)
                                                                                 gggTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGGACGGGCGGGCCGGCCGGTCTgg              >  2:6305736/1‑100 (MQ=255)
                                                                                  ggTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGGACGGGCGGCCCGGGCGGTCTGCg             >  1:1092477/1‑101 (MQ=255)
                                                                                  ggTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGGCCCGCCGacacgcc             >  2:6922646/1‑95 (MQ=255)
                                                                                   gTCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCgg            >  1:2432378/1‑101 (MQ=255)
                                                                                    tCTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGc           >  1:4795129/1‑101 (MQ=255)
                                                                                     cTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt          >  2:1303242/1‑101 (MQ=255)
                                                                                     cTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt          <  1:2556754/101‑1 (MQ=255)
                                                                                     cTGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt          <  1:2082665/101‑1 (MQ=255)
                                                                                      tgcccctgg.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg         <  2:4614548/93‑1 (MQ=255)
                                                                                      tGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg         <  1:4038349/101‑1 (MQ=255)
                                                                                      tGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg         <  2:3258976/101‑1 (MQ=255)
                                                                                      tGACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCCGCCGGGCTGCGGGTg         >  2:3855382/1‑101 (MQ=255)
                                                                                       gACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg        <  2:2788549/101‑1 (MQ=255)
                                                                                       gACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg        <  2:1358999/101‑1 (MQ=255)
                                                                                       gACCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg        <  1:192576/101‑1 (MQ=255)
                                                                                        aCCCTGG.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa       <  2:6279196/101‑1 (MQ=255)
                                                                                          cctgg.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACg     <  2:513898/97‑1 (MQ=255)
                                                                                           ctgg.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg    >  1:1833956/4‑101 (MQ=255)
                                                                                           ctgg.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg    >  1:319917/4‑101 (MQ=255)
                                                                                             gg.CCGAGGCGCA.GCTGGAACCCGCGATGGCAC.GGC.TGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGc  >  1:1186274/2‑101 (MQ=255)
                                                                                                      |                                                                                               
GGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.G.CCGAGG.GCA.G.TGGAA.CCGCGATGG.AA.GGC.T.GCCGAG.TGTTCGCCCGGCAGGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGC  >  NC_014034/3256072‑3256256

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG