Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,180 +C 100% coding (1323/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1801.→C100.0% 862.3 232coding (1323/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (119/113):  ref base (0/0):  total (119/113)

Only 100 of 261 total aligned reads displayed.

GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGG.AA.CCGCGATGG.AA.GGC.T.GCCGAG.T.GTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270
                                                                                                     |                                                                                                    
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AA.c                                                                                                     >  2:2497424/1‑101 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AA.c                                                                                                     <  2:6310497/101‑1 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AA.cccgcg                                                                                                <  2:790076/101‑5 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AA.cccgcg                                                                                                <  2:6386589/101‑5 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AA.cccgcg                                                                                                >  2:3212549/1‑97 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AA.cccgcga                                                                                               >  1:175175/1‑96 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AA.cccgcga                                                                                               >  1:2812783/1‑96 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggc                                                                                            >  1:5178163/1‑100 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggc                                                                                            >  2:6692025/1‑100 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggc                                                                                            >  1:1659836/1‑100 (MQ=255)
           tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggca                                                                                           <  1:839814/101‑3 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGCAC.CCGCGATggcac                                                                                          >  2:6208707/1‑98 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcac                                                                                          <  2:4819350/101‑4 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcac                                                                                          <  2:5551756/101‑4 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacg                                                                                         <  1:5859982/101‑5 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacg                                                                                         <  2:3032995/101‑5 (MQ=255)
               gcgcGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggc                                                                                       >  2:1550919/1‑95 (MQ=255)
                cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggct                                                                                      <  2:3191098/101‑8 (MQ=255)
                cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggct                                                                                      <  2:6636247/101‑8 (MQ=255)
                cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggct                                                                                      <  1:5693265/101‑8 (MQ=255)
                cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggct                                                                                      >  2:4453042/1‑94 (MQ=255)
                        ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.t                                                                           <  2:4075793/101‑1 (MQ=255)
                         gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.tt                                                                          <  2:5784712/101‑2 (MQ=255)
                         gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.tt                                                                          >  2:5989897/1‑100 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.ttg                                                                         >  2:1770343/1‑99 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGG.CACGGC.TGGCCGAg.ttg                                                                         <  1:415225/101‑3 (MQ=255)
                           caccgggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.ttgc                                                                        >  2:3559436/5‑98 (MQ=255)
                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.ttgct                                                                       >  1:5440643/1‑97 (MQ=255)
                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.ttgct                                                                       >  2:922705/1‑97 (MQ=255)
                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTAG.AACCCGCGATGGCAC.GGC.TGGCCGAg.ttgct                                                                       >  2:5254916/1‑97 (MQ=255)
                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.ttgctc                                                                      >  1:5012577/1‑96 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.ttgctcgc                                                                    >  1:7097490/1‑94 (MQ=255)
                                ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAg.ttgctcgcc                                                                   >  2:4743530/1‑93 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGG.CACGGC.TGGCCGAGTT.GCTCGCCCgg                                                                <  1:5530528/101‑1 (MQ=255)
                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCggga                                                              >  1:4614787/1‑99 (MQ=255)
                                      tGCTCAAGGGCGGCGGGCGCGGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..g                                                           <  2:1024542/101‑1 (MQ=255)
                                      tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGccg                                                             >  1:1579198/1‑99 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGG.CACGGC.TGGCCGAGTT.GCTCGCCCGGCA..gg                                                          <  1:1306687/101‑1 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..ggg                                                         >  2:4165839/1‑101 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCC..gggg                                                        >  2:3332036/1‑100 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGgc                                                        <  1:2516932/101‑1 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGgcg                                                       >  2:4428744/1‑101 (MQ=255)
                                                cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCCC.gggca                                                >  1:513910/1‑98 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGC.ggggcc                                               >  2:3702563/1‑97 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGC.gggcac                                               <  1:2425670/101‑5 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGC.gggcac                                               <  2:4461673/101‑5 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGC.gggcac                                               <  2:1659775/101‑5 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGC.gggcacg                                              <  2:5719663/101‑6 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGC.gggcacg                                              <  1:3832559/101‑6 (MQ=255)
                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCAc.gg                                             >  1:2304350/1‑101 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCAc.ggg                                            >  1:6572999/1‑100 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCAc.gggc                                           <  2:1497878/101‑3 (MQ=255)
                                                     cgatctgCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCAc.gggc                                           <  1:7075943/95‑3 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                        <  2:2231129/101‑6 (MQ=255)
                                                          ctcATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                       <  2:4392502/99‑1 (MQ=255)
                                                          cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                       <  2:206261/101‑1 (MQ=255)
                                                          cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                       >  1:884256/1‑101 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCCGCA..GGGCGCGGGCAc.gggcagatcg                                     >  2:7075569/1‑93 (MQ=255)
                                                            cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccag                                     >  1:4392724/1‑99 (MQ=255)
                                                              tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCAGGGGGCGG.GGCCG.GGcggcccggcc                                   >  2:2680569/1‑101 (MQ=255)
                                                              tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggcc                                   <  1:1027345/101‑1 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGGGGCCCGGCCggt                                >  1:6036971/1‑99 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTT.GCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                              <  2:4962909/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                              >  2:5421732/1‑101 (MQ=255)
                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCggcctg                             >  1:6627288/1‑96 (MQ=255)
                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTg                             >  1:4470165/1‑101 (MQ=255)
                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGGACGGGCGGCCCGGGCGGTCTGCg                           >  1:1092477/1‑101 (MQ=255)
                                                                        tCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGc                         >  1:4795129/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGGTCTGCgggc                        >  1:6938435/1‑99 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                        >  2:1303242/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                        <  1:3988161/101‑1 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                        <  2:5378002/101‑1 (MQ=255)
                                                                          tgcccctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                       <  2:4614548/93‑1 (MQ=255)
                                                                          tGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                       >  1:627363/1‑101 (MQ=255)
                                                                          tGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                       <  2:1792899/101‑1 (MQ=255)
                                                                          tGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                       <  1:2683900/101‑1 (MQ=255)
                                                                          tGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                       <  2:3258976/101‑1 (MQ=255)
                                                                           gACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                      <  1:192576/101‑1 (MQ=255)
                                                                           gACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                      <  2:1358999/101‑1 (MQ=255)
                                                                           gACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                      <  2:2788549/101‑1 (MQ=255)
                                                                            aCCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                     <  2:6279196/101‑1 (MQ=255)
                                                                             ccctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                    <  1:3332152/96‑1 (MQ=255)
                                                                              cctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACg                   <  2:513898/97‑1 (MQ=255)
                                                                               ctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                  >  1:1833956/4‑101 (MQ=255)
                                                                               ctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                  >  1:319917/4‑101 (MQ=255)
                                                                                 ggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGc                >  1:1186274/2‑101 (MQ=255)
                                                                                     gaggcgcagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt            <  2:117851/91‑1 (MQ=255)
                                                                                     gaggcgcagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt            <  1:2174913/91‑1 (MQ=255)
                                                                                      aggcgcagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg           <  1:147724/92‑1 (MQ=255)
                                                                                      aggcgcagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg           <  1:6239184/92‑1 (MQ=255)
                                                                                        gcgcagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAG.TCGCTCGCCCGGCA..GGGCGCGGGCACGGGTGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt         <  1:6414638/94‑1 (MQ=255)
                                                                                         cgcagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg        <  2:210820/95‑1 (MQ=255)
                                                                                          gcagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGAGGc       >  2:6870533/6‑101 (MQ=255)
                                                                                           cagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc      <  1:2942168/97‑1 (MQ=255)
                                                                                           cagctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc      <  1:6213902/97‑1 (MQ=255)
                                                                                            agctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCGCCTGCTGCCg     >  2:1837629/4‑101 (MQ=255)
                                                                                             gctGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGCCCTGGTGCCgg    >  2:3743834/3‑101 (MQ=255)
                                                                                             g.TGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg   <  2:2304255/101‑1 (MQ=255)
                                                                                               tGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCCCGGGCCGCCCCGCCGAACTGCGGCTGGACGGGCTCCCGATTCCgggg  >  1:3659866/1‑100 (MQ=255)
                                                                                               tGG.AACCCGCGATGGCAC.GGC.TGGCCGAGTT.GCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCcggg  >  2:4300938/1‑100 (MQ=255)
                                                                                                     |                                                                                                    
GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGG.AA.CCGCGATGG.AA.GGC.T.GCCGAG.T.GTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG