Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,201 +T 100% coding (1344/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2011.→T100.0% 787.0 207coding (1344/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (96/110):  ref base (0/1):  total (96/111)

Only 100 of 244 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCA.G.TGGA.A.CCGCGATGG.AA.GGC.T.GCCGA.G.TGTTCGCCCGGCA..GGGCGC.G.GCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256108‑3256293
                                                                                                         |                                                                                                 
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.tt                                                                                                 <  2:5784712/101‑2 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttg                                                                                                >  2:1770343/1‑99 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGG.CACGGC.TGGCCGA.g.ttg                                                                                                <  1:415225/101‑3 (MQ=255)
   cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgc                                                                                               >  1:64125/1‑98 (MQ=255)
   cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGG.CACGGC.TGGCCGA.g.ttgc                                                                                               <  2:3631918/101‑4 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgct                                                                                              >  1:300238/1‑97 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgcc                                                                                              >  2:3286377/1‑97 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGTA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgctc                                                                                             >  1:5102091/1‑96 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgctc                                                                                             >  1:5012577/1‑96 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgctcgc                                                                                           >  1:7097490/1‑94 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgctcgc                                                                                           <  2:1221529/101‑8 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgctcgcc                                                                                          >  2:4743530/1‑93 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.g.ttgctcgcc                                                                                          >  2:5490081/1‑93 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGG.CACGGC.TGGCCGA.g.ttgctcgcc                                                                                          <  1:4191480/101‑9 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGG.CACGGC.TGGCCGA.GTTGCTCGCCCg                                                                                         <  2:4008791/101‑1 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCgg                                                                                        >  2:444503/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCgg                                                                                        >  2:5129561/1‑101 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGc                                                                                       <  2:2211948/101‑1 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCggga                                                                                      >  1:4614787/1‑99 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCa                                                                                      <  1:1732741/101‑1 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCa                                                                                      <  1:1955535/101‑1 (MQ=255)
              tGCTCAAGGGCGGCGGGCGCGGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..g                                                                                   <  2:1024542/101‑1 (MQ=255)
              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGccg                                                                                     >  1:1579198/1‑99 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGG.CACGGC.TGGCCGA.GTTGCTCGCCCGGCA..gg                                                                                  <  1:1306687/101‑1 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCC..ggg                                                                                 >  2:3199312/1‑101 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..ggg                                                                                 <  2:83071/101‑1 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..ggg                                                                                 >  2:620040/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGAGG.TGCTCGcccccccgggg                                                                                  >  1:3758389/1‑93 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..gggg                                                                                >  2:5646655/1‑100 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGgc                                                                                <  1:5873863/101‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGgcg                                                                               >  2:4428744/1‑101 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGgcg                                                                               >  1:2788651/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGGTCGCCCGGCA..GGgcgc                                                                              >  2:2064687/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCC..GGgggc                                                                              >  2:4251784/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGgcgc                                                                              >  1:1874415/1‑101 (MQ=255)
                      ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGgggccgg                                                                           >  1:1439007/1‑98 (MQ=255)
                        cggcggGGACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCCGCA..GGGCGC.g.ggga                                                                       >  2:6238865/1‑98 (MQ=255)
                        cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGA.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.g.ggta                                                                       <  1:6255886/101‑4 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.g.gggcc                                                                      >  2:3702563/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.g.ggcac                                                                      <  2:5279246/101‑5 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.g.ggcac                                                                      <  1:2425670/101‑5 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCAc.gg                                                                    <  1:5776530/101‑1 (MQ=255)
                            gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCAc.ggg                                                                   >  1:6572999/1‑100 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCAc.gggc                                                                  <  2:1497878/101‑3 (MQ=255)
                             cgatctgCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCAc.gggc                                                                  <  1:7075943/95‑3 (MQ=255)
                                gccGCATGGCGGCGGGCCTGCCCCTGGCCGAGGCGCA.GCTGGAGC.CCGCGATGGCAC.GGCGG.GCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCAc.gggcggc                                                               <  2:1358166/100‑6 (MQ=255)
                                aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCAc.gggcggc                                                               <  2:4656725/101‑6 (MQ=255)
                                 ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCCGCA..GGGCGCGG.GCACGGGcggcc                                                               >  2:5419697/1‑101 (MQ=255)
                                  ctcATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggccc                                                              <  2:4392502/99‑1 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggccc                                                              <  1:5146439/101‑1 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggccc                                                              <  1:1513943/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggcccgg                                                            <  2:6862623/101‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggcccggc                                                           <  2:300234/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCATC.TGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggcccggcc                                                          <  1:6330146/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCAGGGGGCGG.G.GCCG.GGcggcccggcc                                                          >  2:2680569/1‑101 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggcccggcc                                                          <  1:1027345/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggcccggcc                                                          <  1:3987319/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGcggcccggcc                                                          <  2:6387128/101‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCg                                                         <  1:3559572/101‑1 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTT.GCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCt                                                     <  2:4962909/101‑1 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCt                                                     <  2:2302195/101‑1 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTg                                                    >  1:4470165/1‑101 (MQ=255)
                                             gggTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GGACGGGCGGGCCGGCCGGTCTgg                                                   >  2:6305736/1‑100 (MQ=255)
                                              ggTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGGCCCGCCGacacgcc                                                  >  2:6922646/1‑95 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGc                                                >  1:4795129/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGc                                                >  1:6223041/1‑101 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGGCCGGCCGATCTGCGGcc                                               >  1:1148302/1‑100 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGGTCTGCgggc                                               >  1:6938435/1‑99 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCt                                               <  1:99969/101‑1 (MQ=255)
                                                  tgcccctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTg                                              <  2:4614548/93‑1 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTg                                              <  1:2683900/101‑1 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCCGCCGGGCTGCGGGTg                                              >  2:3855382/1‑101 (MQ=255)
                                                    aCCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGa                                            <  2:6279196/101‑1 (MQ=255)
                                                     ccctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                           <  2:3239562/96‑1 (MQ=255)
                                                      cctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCg                                          >  1:5490030/5‑101 (MQ=255)
                                                       ctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                                         >  1:1833956/4‑101 (MQ=255)
                                                       ctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                                         >  1:319917/4‑101 (MQ=255)
                                                              gaggcgcagctGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  1:2174913/91‑1 (MQ=255)
                                                              gaggcgcagctGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  1:5422004/91‑1 (MQ=255)
                                                               aggcgcagctGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                  <  2:1833892/92‑1 (MQ=255)
                                                                    cagctGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  1:6605459/97‑1 (MQ=255)
                                                                    cagctGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  1:2321876/97‑1 (MQ=255)
                                                                      gctGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCgg                           >  1:2546281/3‑101 (MQ=255)
                                                                      gctGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcccg                           >  2:5226298/3‑99 (MQ=255)
                                                                       ctGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGGTGGCggg                          >  2:1904900/2‑101 (MQ=255)
                                                                        tGGA.ACCCGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCcggg                         >  2:4300938/1‑100 (MQ=255)
                                                                          ggaacccGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg                        >  1:2550395/6‑101 (MQ=255)
                                                                            aacccGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      <  1:365937/98‑1 (MQ=255)
                                                                            aacccGCGATGG.CACGGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGGTCTGCGGCTGGACGGGGTCCCGATGCCGGGcgcc                      >  1:2901961/4‑101 (MQ=255)
                                                                               ccGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  2:7097116/101‑1 (MQ=255)
                                                                               ccGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  2:2473274/101‑1 (MQ=255)
                                                                               ccGCGATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.GCGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  2:1124075/101‑1 (MQ=255)
                                                                                cgcgATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCCGGGGCCCCGa                  >  1:1070471/1‑101 (MQ=255)
                                                                                 gcgATGGCAC.GGC.TGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                 <  1:4287288/101‑1 (MQ=255)
                                                                                            cggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt        <  2:2901852/95‑1 (MQ=255)
                                                                                             ggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGATg       <  2:884232/96‑1 (MQ=255)
                                                                                              gctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGa      <  1:820241/97‑1 (MQ=255)
                                                                                                tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    >  2:6693539/3‑101 (MQ=255)
                                                                                                tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    >  1:4902351/3‑101 (MQ=255)
                                                                                                 ggCCGA.GTTGCTCGCCCGGCA..GGGCGCGG.GCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCCCGCTGATCg   >  1:617467/2‑101 (MQ=255)
                                                                                                         |                                                                                                 
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCA.G.TGGA.A.CCGCGATGG.AA.GGC.T.GCCGA.G.TGTTCGCCCGGCA..GGGCGC.G.GCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256108‑3256293

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG