Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,275,790 +G 1.7% intergenic (‑66/+90) nrdR ← / ← RCAP_rcc03082 transcriptional regulator NrdR/lipoprotein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,275,7901.→G 1.7% 7.4 ‑0.4 ‑0.1290intergenic (‑66/+90)nrdR/RCAP_rcc03082transcriptional regulator NrdR/lipoprotein
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (155/130):  total (157/133)
Fisher's exact test strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.16e-01

Only 100 of 376 total aligned reads displayed.

TGCGTATCGACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCTTTGG  >  NC_014034/3275690‑3275890
                                                                                                     |                                                                                                     
 gCGTATCGACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.g                                                                                                      <  2:6142283/101‑1 (MQ=255)
 gCGTATCGACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.g                                                                                                      <  2:2423870/101‑1 (MQ=255)
 gCGTATCGACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.g                                                                                                      >  2:4835230/1‑101 (MQ=255)
  cGTATCGACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.gg                                                                                                     >  1:1390890/1‑101 (MQ=255)
  cGTATCGACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.gg                                                                                                     >  2:235742/1‑101 (MQ=255)
   gTATCGACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.ggg                                                                                                    <  2:4707713/101‑1 (MQ=255)
       cGACATTTCCGCAAAACGGCCAGCGCATGGCATCCCCCCTCTCATGTTCCCGCCTCATTTGGGGCCTGTCGCCCCGGTTATCCACAGGACTATA.gcggggg                                                                                                >  2:3315042/1‑101 (MQ=255)
        gACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.gggggggg                                                                                               <  1:6748571/101‑1 (MQ=255)
        gACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.gggggggg                                                                                               <  2:1398986/101‑1 (MQ=255)
         gcctttCCGCAAAACGGGCAGCGCATGGCCTCCCCCCTCTCATGTTTCCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.ggggggggg                                                                                              <  2:2053753/98‑1 (MQ=255)
           ctttCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCg                                                                                            <  1:5702963/100‑1 (MQ=255)
           aTTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCg                                                                                            <  1:1458966/101‑1 (MQ=255)
            tttCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGa                                                                                           <  1:304761/101‑1 (MQ=255)
              tCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAgg                                                                                         <  1:3879832/101‑1 (MQ=255)
               ccGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAggg                                                                                        >  2:5734868/1‑101 (MQ=255)
                cGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGa                                                                                       <  2:2722148/101‑1 (MQ=255)
                cGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGa                                                                                       <  2:6992937/101‑1 (MQ=255)
                 gCAAAACGGGCAGCGCATGGCATCCCCCTCCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGAt                                                                                      <  1:6183295/101‑1 (MQ=255)
                  cAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATc                                                                                     >  2:5622651/1‑101 (MQ=255)
                  cAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATc                                                                                     <  2:7065080/101‑1 (MQ=255)
                   aaaaCGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCg                                                                                    >  2:5660900/1‑101 (MQ=255)
                     aaCGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGGTCggg                                                                                  >  1:1885070/1‑101 (MQ=255)
                     aaCGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCggg                                                                                  >  2:3871040/1‑101 (MQ=255)
                      aCGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGt                                                                                 >  1:1671074/1‑101 (MQ=255)
                       cGGGCGGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTCTCCACAGGAGTGTA.GGGGGGGGGCGAGGGCTTgggga                                                                                >  2:3081901/1‑99 (MQ=255)
                       cGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGCGGGATCGGGTa                                                                                >  1:4228352/1‑101 (MQ=255)
                            aGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGcaca                                                                           >  1:1920693/1‑101 (MQ=255)
                            aGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGcaca                                                                           >  2:2567174/1‑101 (MQ=255)
                             gcgcATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACaa                                                                          <  2:5495714/101‑1 (MQ=255)
                              cgcATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACaaa                                                                         <  1:6796673/101‑1 (MQ=255)
                              cgcATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACaaa                                                                         >  1:4108669/1‑101 (MQ=255)
                               gcATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAAt                                                                        <  2:789498/101‑1 (MQ=255)
                               gcATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGCGGGGGGCGAGGGATCGGGTAGCCCACAt                                                                        >  1:6030863/1‑101 (MQ=255)
                                cATGGCATCCCCCCTCTCATGTTTCCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAAtt                                                                       <  2:1314495/101‑1 (MQ=255)
                                 aTGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTg                                                                      >  2:6237579/1‑101 (MQ=255)
                                 aTGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGGAGCACAAATTg                                                                      >  2:1735596/1‑101 (MQ=255)
                                  tGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTgg                                                                     >  1:754648/1‑101 (MQ=255)
                                           cccTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCa                                                           >  1:1742861/1‑101 (MQ=255)
                                              cctcATGTTACCGCCTTATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGAt                                                        <  2:5193429/100‑1 (MQ=255)
                                               ctcATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATa                                                       <  2:3160910/101‑1 (MQ=255)
                                               ctcATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATa                                                       >  2:6702194/1‑101 (MQ=255)
                                                tcATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGGAGCACAAATTGGGGG.CCCCCAGAtcc                                                      >  2:34748/1‑99 (MQ=255)
                                                 cATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACg                                                     <  1:1987876/101‑1 (MQ=255)
                                                  aTGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.cccagatcggaag                                                    <  1:5443417/101‑11 (MQ=255)
                                                   tGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGCAGCACAAATTGGCGG.CCCCCACATACggg                                                   >  1:2164791/1‑101 (MQ=255)
                                                    gTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACgggg                                                  >  2:6533457/1‑101 (MQ=255)
                                                     ttACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGc                                                 <  2:4043606/101‑1 (MQ=255)
                                                      tACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGGGGGATCGGGTAGGACCAAATGGGGG.GCCCCAGGTACgggggc                                                >  1:5636457/1‑99 (MQ=255)
                                                       tccGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCg                                               <  2:6907420/100‑1 (MQ=255)
                                                       aCCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCg                                               >  1:283759/1‑101 (MQ=255)
                                                         cGCCTCATTTGGGTTCTGTCTCCCCGGTTATCCACAGTACTATA.GGGGTGGGGCGAGGGATCTGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGa                                             <  2:1470689/101‑1 (MQ=255)
                                                          gCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGaa                                            <  2:7127199/101‑1 (MQ=255)
                                                           ccTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAAc                                           >  2:2193686/1‑101 (MQ=255)
                                                            cTCATTTGGGGGCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGGTCGGGTAGCACAAATTGGCGGACCCGCAG.TACGGGGCCGGGACt                                          >  1:5854050/1‑101 (MQ=255)
                                                              cATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACtgg                                        >  2:4216504/1‑100 (MQ=255)
                                                               aTTGGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACtgtg                                       <  2:4122161/101‑1 (MQ=255)
                                                                 ttGGGGGCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTggc                                     <  1:4898404/101‑1 (MQ=255)
                                                                      gTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGgcgc                                >  1:6373615/1‑101 (MQ=255)
                                                                        cTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGACGGATCGGGGAGCACAAATTGGCGG.CCCCCGGATACGGAGCCGGTACTCTGGCGGcgcgc                              >  2:4668086/1‑100 (MQ=255)
                                                                         tGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGa                             <  1:5898165/101‑1 (MQ=255)
                                                                          gTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGAc                            <  2:1671013/101‑1 (MQ=255)
                                                                          gTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGAc                            <  2:7003310/101‑1 (MQ=255)
                                                                            cGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTc                          <  1:3162437/101‑1 (MQ=255)
                                                                            cGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTc                          <  1:2508185/101‑1 (MQ=255)
                                                                               cccGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTGAGc                       >  1:799366/1‑101 (MQ=255)
                                                                                ccGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGcc                      >  2:5339764/1‑101 (MQ=255)
                                                                                  ggTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCcgc                    <  1:4669700/101‑1 (MQ=255)
                                                                                   gTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCcgcg                   >  1:3790589/1‑101 (MQ=255)
                                                                                   gTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCcgcg                   >  1:979906/1‑101 (MQ=255)
                                                                                   gTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCcgcg                   <  2:4471178/101‑1 (MQ=255)
                                                                                   gTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCcgcg                   >  2:4384474/1‑101 (MQ=255)
                                                                                   gTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCcgcg                   >  2:2153999/1‑101 (MQ=255)
                                                                                     tATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGAc                 >  1:1319654/1‑101 (MQ=255)
                                                                                      aTCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGAcc                >  1:7001363/1‑101 (MQ=255)
                                                                                         cacaGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGc             >  1:5239825/1‑101 (MQ=255)
                                                                                          acaGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCt            <  2:6014112/101‑1 (MQ=255)
                                                                                           caGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTTGCGGCGCGGACTCAGCCGCGACCGGCTc           <  1:5066877/101‑1 (MQ=255)
                                                                                            aGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCa          <  2:5857297/101‑1 (MQ=255)
                                                                                            aGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCa          <  2:1907995/101‑1 (MQ=255)
                                                                                             ggACTATAGGGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCa          <  1:4590785/101‑1 (MQ=255)
                                                                                             ggACTATA.GGGGGGTGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAg         <  2:460093/101‑1 (MQ=255)
                                                                                             ggACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAg         >  1:2876844/1‑101 (MQ=255)
                                                                                             ggACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAg         <  2:3576051/101‑1 (MQ=255)
                                                                                             ggACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAg         >  1:3680007/1‑101 (MQ=255)
                                                                                              gACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAgg        <  1:514305/101‑1 (MQ=255)
                                                                                              gACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAgg        <  1:2642979/101‑1 (MQ=255)
                                                                                              gACTATA.GGGGGGGGGCGAGGAATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAgg        <  2:3790428/101‑1 (MQ=255)
                                                                                               aCTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGc       <  2:6313383/101‑1 (MQ=255)
                                                                                                cTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCt      >  1:1090309/1‑101 (MQ=255)
                                                                                                 tata.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGGCTCAGCCGCGACCGGCTCAGGCtt     >  2:3691493/1‑101 (MQ=255)
                                                                                                 tata.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCtt     <  1:1015358/101‑1 (MQ=255)
                                                                                                 tata.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCtt     >  1:629819/1‑101 (MQ=255)
                                                                                                 tata.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCtt     >  1:926297/1‑101 (MQ=255)
                                                                                                  ata.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCttt    <  1:3977105/101‑1 (MQ=255)
                                                                                                  ata.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCttt    <  1:1714328/101‑1 (MQ=255)
                                                                                                  ata.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCttt    >  2:4934186/1‑101 (MQ=255)
                                                                                                  ata.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCttt    <  2:5066988/101‑1 (MQ=255)
                                                                                                   ta.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCTTTg   >  2:1228463/1‑101 (MQ=255)
                                                                                                    a.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCTTTgg  >  1:4737739/1‑101 (MQ=255)
                                                                                                    a.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCTTTgg  <  1:3390113/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
TGCGTATCGACATTTCCGCAAAACGGGCAGCGCATGGCATCCCCCCTCTCATGTTACCGCCTCATTTGGGGTCTGTCGCCCCGGTTATCCACAGGACTATA.GGGGGGGGGCGAGGGATCGGGTAGCACAAATTGGCGG.CCCCCAGATACGGGGCCGGAACTGTGGCGGCGCGGACTCAGCCGCGACCGGCTCAGGCTTTGG  >  NC_014034/3275690‑3275890

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG