Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,328,528 +G 100% intergenic (‑49/+11) entB ← / ← argF isochorismatase/ornithine carbamoyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,328,5281.→G100.0% 1121.9 336intergenic (‑49/+11)entB/argFisochorismatase/ornithine carbamoyltransferase
Reads supporting (aligned to +/- strand):  new base (205/114):  ref base (0/3):  total (206/130)

Only 100 of 388 total aligned reads displayed.

GCGCCTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.A.CCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGCCC  >  NC_014034/3328428‑3328622
                                                                                                      |                                                                                               
gcgcCTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCaa.a                                                                                                  <  1:4601960/101‑1 (MQ=255)
gcgcCTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCaa.a                                                                                                  <  1:6401796/101‑1 (MQ=255)
  gcCTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCaa.agc                                                                                                >  1:4184091/1‑99 (MQ=255)
   ccTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.A.gcc                                                                                              >  2:6402337/1‑101 (MQ=255)
   ccTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.A.gcc                                                                                              >  1:1059758/1‑101 (MQ=255)
      gCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGCGTCTGGGGGCAA.A.gccccc                                                                                           >  2:1170571/1‑100 (MQ=255)
       cATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTCCCCATCGCCGTCCCTTTCATCTTGCCATAAATATCCGGGGGGTCTGGGGGCAA.A.gccccca                                                                                          <  2:6877867/101‑3 (MQ=37)
        aTCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.Ag                                                                                         <  2:5082565/101‑1 (MQ=255)
        aTCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCCTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGTCAA.AGCCCCC.Ag                                                                                         <  1:5054418/101‑1 (MQ=255)
            cGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCtt                                                                                     <  1:1188005/101‑1 (MQ=255)
            cGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCtt                                                                                     >  1:2822057/1‑101 (MQ=255)
              cTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCa                                                                                   <  1:4443326/101‑1 (MQ=255)
              cTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCa                                                                                   >  1:360511/1‑101 (MQ=255)
              cTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCa                                                                                   <  1:762945/101‑1 (MQ=255)
              cTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCa                                                                                   >  2:6725278/1‑101 (MQ=255)
               tttGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCAc                                                                                  >  2:1508895/1‑101 (MQ=255)
               tttGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCAc                                                                                  >  1:1608234/1‑101 (MQ=255)
                   cATGTGGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGc                                                                              >  2:3516822/1‑101 (MQ=255)
                   cATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGc                                                                              >  1:3098787/1‑101 (MQ=255)
                   cATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTCCTTTTCATCTTGCCATAAATATCCGGGGGGTCGGGGGGCAA.AGCCCCC.AGCCTTCACTTGc                                                                              <  2:1728463/101‑1 (MQ=37)
                    aTGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGcc                                                                             >  1:6014268/1‑101 (MQ=255)
                     tGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGccc                                                                            <  2:4192529/101‑1 (MQ=255)
                     tGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGccc                                                                            >  2:5674627/1‑101 (MQ=255)
                      gTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCa                                                                           <  1:6795694/101‑1 (MQ=255)
                        gaaagatCAAAAGCGGGGCTTTTCCCTTCGCCAGGCCTTTAACCTTGCTATAAATTTCCTGGGGCTCTGGGGGAAA.ACCCCCC.AGCCTTCACTTGCCCaaa                                                                         <  2:4816392/97‑1 (MQ=255)
                        cGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTTGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCaaa                                                                         >  1:3536393/1‑101 (MQ=255)
                        cGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCaaa                                                                         >  1:5778413/1‑101 (MQ=255)
                         gatgatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAg                                                                        >  2:3560653/1‑101 (MQ=255)
                          atgatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGc                                                                       >  1:1420623/1‑101 (MQ=255)
                            gatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGccc                                                                     >  1:7090327/1‑101 (MQ=255)
                            gatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGccc                                                                     <  2:4942550/101‑1 (MQ=255)
                            gatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGccc                                                                     <  2:6520888/101‑1 (MQ=255)
                            gatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGccc                                                                     >  2:3637348/1‑101 (MQ=255)
                               cAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAgc                                                                  >  2:5576496/1‑101 (MQ=255)
                                 gAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAgcgc                                                                >  2:180306/1‑101 (MQ=255)
                                  aaGCGCGGTCTTGCCCACCGCCGTGCTTTTCTTCTTCCCAAAAATACCGTGGGGGGCGGGGGGCAA.ACCCCCC.GGCCTTCACTTGCCCAAAGCCCAGCGCa                                                               <  2:6015342/101‑1 (MQ=255)
                                   aGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCAt                                                              <  2:5352392/101‑1 (MQ=255)
                                    gcgcGTTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.ACCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATg                                                             <  2:5924651/101‑1 (MQ=255)
                                    gcccgGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATg                                                             <  2:3826131/98‑1 (MQ=255)
                                     cgcgGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGa                                                            >  1:6214589/1‑101 (MQ=255)
                                      gcgGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGAt                                                           <  2:4002005/101‑1 (MQ=255)
                                        ggTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCg                                                         >  1:2240248/1‑101 (MQ=255)
                                        ggTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCACC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCg                                                         >  1:4874152/1‑101 (MQ=255)
                                          tCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGcc                                                       >  2:1125252/1‑101 (MQ=255)
                                          tCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGcc                                                       >  2:2129692/1‑101 (MQ=255)
                                            ttccccATCCCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCtt                                                     <  2:565422/98‑1 (MQ=39)
                                             tGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCttt                                                    <  2:6154788/101‑1 (MQ=255)
                                              gCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCtttt                                                   <  2:5503334/101‑1 (MQ=255)
                                                 cATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTgcg                                                >  1:3790915/1‑101 (MQ=255)
                                                     gCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATg                                            <  1:4513960/101‑1 (MQ=255)
                                                      ccttGCTTTTCATCTTGCAATAAATATCCGGGGGGTCGGGGGGCAACC.CCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGc                                           <  1:2472207/98‑1 (MQ=255)
                                                       cgtccttttCATCTTGCCATAAATATCCGGGGGGTCGGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCa                                          <  2:1625441/95‑1 (MQ=37)
                                                       cGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCa                                          >  1:6691842/1‑101 (MQ=255)
                                                          gCCTTTCATCTTGCCATAACTATCCTGGGGGTCTGGGGGCAA.ACCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGcc                                       <  2:2234631/101‑1 (MQ=255)
                                                          gCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGcc                                       >  2:1090675/1‑101 (MQ=255)
                                                           ccTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCg                                      <  2:6277127/101‑1 (MQ=255)
                                                           ccTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCg                                      >  1:5368090/1‑101 (MQ=255)
                                                           ccTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCg                                      <  1:4336887/101‑1 (MQ=255)
                                                             tttCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCACGAGCTCCTTTTGCGCATGCAGCCgat                                    >  2:4595167/1‑99 (MQ=38)
                                                              ttCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGtt                                   >  1:5503467/1‑101 (MQ=255)
                                                               tCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTtc                                  <  2:6691025/101‑1 (MQ=255)
                                                               tCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTtc                                  >  2:4738057/1‑101 (MQ=255)
                                                               tCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTtc                                  <  1:4240484/101‑1 (MQ=255)
                                                                  tcgtcccATAAATCTCCTGGGGGTCGGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTcc                               <  2:3291575/96‑1 (MQ=255)
                                                                  tCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTcc                               <  2:5426829/101‑1 (MQ=255)
                                                                  tCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTcc                               <  1:6310934/101‑1 (MQ=255)
                                                                  tCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTcc                               >  1:2778065/1‑101 (MQ=255)
                                                                   cTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCg                              >  2:86032/1‑101 (MQ=255)
                                                                    ttGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGc                             >  2:2725229/1‑101 (MQ=255)
                                                                    ttGCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGc                             >  1:1787749/1‑101 (MQ=255)
                                                                      gCCATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCt                           >  1:2407693/1‑101 (MQ=255)
                                                                       ccATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtc                          <  2:7114235/101‑1 (MQ=255)
                                                                       ccATAAATATCCGGGGGGTCTGGGGGCAA.ACCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGAATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtc                          <  1:2591964/101‑1 (MQ=255)
                                                                        cATAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcg                         >  2:2955612/1‑101 (MQ=255)
                                                                          tAAATATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtc                       >  2:4111672/1‑101 (MQ=255)
                                                                           aaaTATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtcg                      >  1:3276291/1‑101 (MQ=255)
                                                                           aaaTATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtcg                      >  2:1418464/1‑101 (MQ=255)
                                                                           aaaTATCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtcg                      >  2:1347131/1‑101 (MQ=255)
                                                                               ttCCTGGGGGACAGGGGGGAA.AGCTCCC.GGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGa                  <  2:4260906/100‑1 (MQ=255)
                                                                               atCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGa                  >  2:524173/1‑101 (MQ=255)
                                                                               atCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGa                  >  2:1029863/1‑101 (MQ=255)
                                                                               atCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGa                  >  1:5647466/1‑101 (MQ=255)
                                                                               atCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGa                  >  1:6654465/1‑101 (MQ=255)
                                                                                tCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGAt                 >  1:1124958/1‑101 (MQ=255)
                                                                                tCCTGGGGGTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGAt                 >  1:6947412/1‑101 (MQ=255)
                                                                                   gcggggTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCAc              <  1:3885895/99‑1 (MQ=255)
                                                                                    gggggTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCTTGCAGCCGGTTCTCCGCCTCGTCGAAGATCAcc             <  1:3700136/101‑1 (MQ=255)
                                                                                    gggggTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCAcc             >  1:3971921/1‑101 (MQ=255)
                                                                                     ggggTCTGGGGGCAA.ATCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCg            >  1:921559/1‑101 (MQ=255)
                                                                                      gggTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGa           >  1:3450025/1‑101 (MQ=255)
                                                                                       ggTCTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGaa          >  2:4415381/1‑101 (MQ=255)
                                                                                        gTCGGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAAt         <  2:576270/101‑1 (MQ=255)
                                                                                        gTCGGGGGCCAA.A.CCCCCGAGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAAt         <  2:1985184/101‑1 (MQ=255)
                                                                                          cTGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGc       >  2:6849304/1‑101 (MQ=255)
                                                                                           tGGGGGCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCg      >  1:5276381/1‑101 (MQ=255)
                                                                                            gggggCAA.AGCCCCC.AGCCTTCACTTGCCCAAAACCCACCGCGCGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCTTCGCCGATCACCGAATGCgg     >  2:3457170/1‑101 (MQ=255)
                                                                                             ggggCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGc    <  2:2688531/101‑1 (MQ=255)
                                                                                              gggCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGcc   >  1:2007923/1‑101 (MQ=255)
                                                                                              gggCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGcc   <  2:3677442/101‑1 (MQ=255)
                                                                                               ggCAA.AGCCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGccc  <  2:2407599/101‑1 (MQ=255)
                                                                                                      |                                                                                               
GCGCCTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.A.CCCCC.AGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGCCC  >  NC_014034/3328428‑3328622

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG