BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,532,987	+G	89.8%	intergenic (‑6/‑19)	RCAP_rcc03340 ← / → RCAP_rcc03341	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,532,987	1	.→G	89.8%	100.0 ‑2.7 ‑1.0	325	intergenic (‑6/‑19)	RCAP_rcc03340/RCAP_rcc03341	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (139/153): ref base (10/23): total (149/176)
Fisher's exact test strand distribution p-value = 6.65e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.03e-01

Only 100 of 359 total aligned reads displayed.


AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.GGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.GGCCC..GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.CTGCGCGCCCGGCAGGGCCGCCAGATCGTGC  >  NC_014034/3532888‑3533080


aGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGc                                                                                                    <  1:840880/101‑1 (MQ=255)


 gCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.g                                                                                                  >  1:6226679/1‑101 (MQ=255)


 gCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.g                                                                                                  <  1:4751525/101‑1 (MQ=255)


  cGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.gg                                                                                                 >  1:6678252/1‑101 (MQ=255)


  cGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.gg                                                                                                 >  1:3112277/1‑101 (MQ=255)


  cGGGGAAAGCGCCGCTTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.gg                                                                                                 <  2:2085740/101‑1 (MQ=255)


    gggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.gggc                                                                                               >  1:5191307/1‑99 (MQ=255)


     ggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcc                                                                                              >  2:2505314/1‑101 (MQ=255)


       aaaGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcccg                                                                                            <  1:4111200/101‑3 (MQ=255)


          gcggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCCGCGCCATCGCGGCGCAAAGCTGC.Gggcccggcc                                                                                         <  1:6371354/101‑6 (MQ=255)


           cggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcccggccc                                                                                        <  2:4835013/101‑7 (MQ=255)


             gcgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.Gggcccggccctt                                                                                      <  1:6833880/101‑9 (MQ=255)


              cgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTc                                                                                     >  1:2066998/1‑101 (MQ=255)


              cgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTc                                                                                     <  1:2332924/101‑1 (MQ=255)


                 cTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGGGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGAt                                                                                  >  1:5096665/1‑101 (MQ=255)


                   gTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATcc                                                                                <  1:1109564/101‑1 (MQ=255)


                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAg                                                                              <  2:2023888/101‑1 (MQ=255)


                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGt                                                                             >  1:5456756/1‑101 (MQ=255)


                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGt                                                                             >  2:5614446/1‑101 (MQ=255)


                       gatccgCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTg                                                                            >  2:6575580/6‑101 (MQ=255)


                       gTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTg                                                                            >  2:18680/1‑101 (MQ=255)


                        tCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGc                                                                           >  1:2457140/1‑101 (MQ=255)


                        cccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGc                                                                           <  1:4918620/100‑1 (MQ=255)


                          cAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAg                                                                         <  1:1568340/101‑1 (MQ=255)


                           aGCCGCCCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGc                                                                        <  2:1418561/101‑1 (MQ=255)


                            gCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGcc                                                                       <  1:2797983/101‑1 (MQ=255)


                             ccgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCa                                                                      <  1:6479302/101‑1 (MQ=255)


                             ccgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCa                                                                      <  2:2844469/101‑1 (MQ=255)


                              cgcccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCag                                                                     <  2:2675547/98‑2 (MQ=255)


                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCag                                                                     <  1:2107845/101‑2 (MQ=255)


                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCag                                                                     >  1:1825561/1‑100 (MQ=255)


                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCagc                                                                    >  2:3771378/1‑99 (MQ=255)


                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCagc                                                                    <  2:3592079/101‑3 (MQ=255)


                                accgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGcc                                                                   <  1:4580969/101‑1 (MQ=255)


                                   gaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTc                                                                >  2:5335210/1‑101 (MQ=255)


                                   gaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTc                                                                <  1:3912205/101‑1 (MQ=255)


                                    aaaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTcc                                                               >  2:2972083/1‑101 (MQ=255)


                                    aaaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTcc                                                               <  2:4626806/101‑1 (MQ=255)


                                    aaaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTcc                                                               <  1:6922871/101‑1 (MQ=255)


                                     aaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCt                                                              <  1:2895010/101‑1 (MQ=255)


                                     aaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCt                                                              <  2:6215610/101‑1 (MQ=255)


                                      agcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTc                                                             <  1:1361011/101‑1 (MQ=255)


                                       gcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCg                                                            >  1:4312198/1‑101 (MQ=255)


                                       gcagcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCg                                                            <  1:4845466/101‑1 (MQ=255)


                                        tcggcctgcatCGGCCAGCCGGGGGGCGGGGGCGATCTGCGCCATCCCGGCGCAAAGCTGCGGGCCCG.GGCCTTCGGTCCACGTCAGGCAACCGTCCCCGa                                                           >  2:3658627/11‑101 (MQ=255)


                                         agcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAc                                                          <  2:3875866/101‑1 (MQ=255)


                                          gcCGCGCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAcc                                                         <  1:610513/101‑1 (MQ=255)


                                            cgcgCCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGc                                                       <  1:1888569/101‑1 (MQ=255)


                                              cgcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCaa                                                     <  1:1984696/101‑1 (MQ=255)


                                               gcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAc                                                    <  1:152004/101‑1 (MQ=255)


                                               gcCTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAc                                                    <  1:365750/101‑1 (MQ=255)


                                               cccTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAc                                                    <  2:1907947/100‑1 (MQ=255)


                                                ccTCGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                   <  2:1593376/101‑1 (MQ=255)


                                                   cGGCCAGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                                <  2:570564/101‑1 (MQ=255)


                                                        aGCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcccc                                           <  2:3056830/101‑1 (MQ=255)


                                                         gCCGGGGGTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCg                                          <  1:2569536/101‑1 (MQ=255)


                                                            gggggTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcac                                       >  2:5039449/1‑101 (MQ=255)


                                                            gggggTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcac                                       <  1:1703955/101‑1 (MQ=255)


                                                             ggggTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcaca                                      >  2:747247/1‑101 (MQ=255)


                                                              gggTCGGGGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAg                                     >  2:3425404/1‑101 (MQ=255)


                                                               ggtcgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGa                                    >  2:6723723/6‑101 (MQ=255)


                                                                 tcgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.c                                <  2:4570193/98‑1 (MQ=255)


                                                                  cgggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cc                               <  1:5282204/99‑2 (MQ=255)


                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cct                              >  2:278687/2‑99 (MQ=255)


                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cct                              <  2:6853412/100‑3 (MQ=255)


                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cct                              <  2:5133005/100‑3 (MQ=255)


                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cct                              >  1:380457/2‑99 (MQ=255)


                                                                   gggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cct                              >  2:6403591/2‑99 (MQ=255)


                                                                    ggggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cctg                             <  2:86212/101‑4 (MQ=255)


                                                                     gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cctgc                            <  2:3357790/101‑5 (MQ=255)


                                                                     gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cctgc                            <  2:2419317/101‑5 (MQ=255)


                                                                      ggCGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.cctgcg                           <  2:1496792/101‑6 (MQ=255)


                                                                        cGATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTgcgcg                          >  1:3356390/1‑101 (MQ=255)


                                                                         gATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTgcgcgc                         >  2:4292614/1‑101 (MQ=255)


                                                                         gATCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTgcgcgc                         >  1:935124/1‑101 (MQ=255)


                                                                          aTCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGcc                        >  2:402486/1‑101 (MQ=255)


                                                                          aTCTGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGcc                        <  2:2243652/101‑1 (MQ=255)


                                                                             tGCGCCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCgg                     >  1:5954590/1‑101 (MQ=255)


                                                                              gcgcCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGc                    <  1:1002733/101‑1 (MQ=255)


                                                                              gcgcCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGc                    <  2:5956628/101‑1 (MQ=255)


                                                                              gcgcCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGc                    >  2:4121790/1‑101 (MQ=255)


                                                                               cgcCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCa                   >  2:5272874/1‑101 (MQ=255)


                                                                               cgcCATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCa                   <  1:5667280/101‑1 (MQ=255)


                                                                                 ccATCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAgg                 <  2:5229565/101‑1 (MQ=255)


                                                                                   aTCGCGGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGgc               <  1:3521626/101‑1 (MQ=255)


                                                                                    tCGCGGCGCAAAGCGGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGgcc              <  2:2732018/101‑1 (MQ=255)


                                                                                     cgcgGCGCAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGgccg             >  2:7095274/1‑101 (MQ=255)


                                                                                     cgcgGCGCAAAGCTGC.GGGCCGGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGgccg             <  2:1101367/101‑1 (MQ=255)


                                                                                         gcgcAAAGGTGC.AGCCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAg         <  2:4506772/101‑1 (MQ=255)


                                                                                          cgcAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGa        >  1:741249/1‑101 (MQ=255)


                                                                                          cgcAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGa        <  2:6046567/101‑1 (MQ=255)


                                                                                            cAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGATc      >  1:3616093/1‑101 (MQ=255)


                                                                                            cAAAGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGACAGGGCCGCCAGATc      <  2:3538827/101‑1 (MQ=255)


                                                                                            cAAAGCTGCGGGCCCG.GCCCTTCGAACCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTAGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGATc      >  2:2374993/1‑101 (MQ=255)


                                                                                             aaaGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGATCg     >  1:5018160/1‑101 (MQ=255)


                                                                                             aaaGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGATCg     >  1:3771081/1‑101 (MQ=255)


                                                                                             aaaGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGATCg     <  2:5455735/101‑1 (MQ=255)


                                                                                             aaaGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGATCg     >  2:182505/1‑101 (MQ=255)


                                                                                             aaaGCTGCGGGCCCG.GCCCTTCGAGCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCAGAGACC.CTGCGCCCCCGGCAGGGCCGCCAGATCg     <  2:5838880/101‑1 (MQ=255)


                                                                                              aaGCTGCGGGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.TCCTGCGCGCCCGGCAGGGCCGCCAGATCGt    >  2:6366778/1‑101 (MQ=255)


AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.GGGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.GGCCC..GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGA.T.CTGCGCGCCCGGCAGGGCCGCCAGATCGTGC  >  NC_014034/3532888‑3533080

Base quality scores:


ATCG

< 3 ≤


ATCG

< 21 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG