Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 408,497 +A 83.5% intergenic (+115/+33) glnA1 → / ← dapA glutamine synthetase/dihydrodipicolinate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034408,4975.→A83.5% 131.9 ‑3.4 ‑0.0248intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (134/73):  ref base (19/22):  total (153/95)
Fisher's exact test strand distribution p-value = 3.45e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.15e-01

Only 100 of 261 total aligned reads displayed.

CTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGCAAAGACGGGGG.....GCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408396‑408571
                                                                                                        |                                                                              
ctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggggg                                                                                 >  1:2640611/1‑101 (MQ=255)
ctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggggg                                                                                 >  2:6310348/1‑101 (MQ=255)
 tGCCCCCAGACCCCCGGGATATTTGGGGCGAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATAGCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggggg.....g                                                                           <  2:848055/101‑1 (MQ=25)
 tGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggggg.....g                                                                           >  1:3350268/1‑101 (MQ=255)
 cgCCCCCAGCCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggggg.....g                                                                           <  2:6135088/100‑1 (MQ=255)
  gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGGAAG.AAAGACGGGGG.....Gc                                                                          >  2:671804/1‑101 (MQ=255)
   tcgcctcaCCCCCGGGATATTTGGGGCAAGATAAACCCCAT.CCTTTCACCTTG.CCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGgggggca                                                                               >  1:516903/8‑99 (MQ=25)
   cccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gca                                                                         >  2:326961/1‑100 (MQ=255)
   cccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gca                                                                         <  2:2029465/101‑2 (MQ=255)
     cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGAGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcaga                                                                       >  2:5253205/1‑98 (MQ=255)
      ccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagag                                                                      <  2:222551/101‑5 (MQ=255)
      ccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAA.CCAAGACGGGGG.....Gcagag                                                                      >  1:5636483/1‑97 (MQ=25)
       cAGACCCCCGGGATATTTGCGGCGAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagc                                                                     <  2:6873001/101‑6 (MQ=25)
       cAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagc                                                                     >  2:5060305/1‑96 (MQ=255)
       cAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagc                                                                     >  2:3091056/1‑96 (MQ=255)
        aGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcc                                                                    <  2:2529362/101‑7 (MQ=255)
        aGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcc                                                                    >  1:2945423/1‑95 (MQ=255)
         gACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagccc                                                                   >  1:1314756/1‑94 (MQ=25)
          aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcccc                                                                  >  2:4378259/1‑93 (MQ=25)
          aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcccc                                                                  <  1:542630/101‑9 (MQ=25)
            ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                <  1:1134826/101‑11 (MQ=25)
            ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                <  2:3884264/101‑11 (MQ=25)
            ccccGGGATATTTGCGGAAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                <  1:6086529/101‑11 (MQ=25)
                        tGCGGCAAGATGAAGCCGATGTCCTTCCTCTTG.CCGCAAATCTCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGcc                                                         <  1:814934/101‑1 (MQ=25)
                        tGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGcc                                                         <  2:2945314/101‑1 (MQ=21)
                        tGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGcc                                                         <  1:4251529/101‑1 (MQ=21)
                           ggCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCaaa                                                      >  1:5978074/1‑101 (MQ=21)
                            gCAAGATTAAGCCGATGTCCTTCATCTTGTCCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCaaa                                                      <  1:6936557/101‑1 (MQ=18)
                            gCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCCCAGGTCGCCaaaa                                                     >  2:2305125/1‑101 (MQ=18)
                             cAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAg                                                    <  2:3645712/101‑1 (MQ=21)
                             cAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAg                                                    <  1:1616334/101‑1 (MQ=21)
                             cAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAg                                                    >  1:3016681/1‑101 (MQ=21)
                              aaGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAgg                                                   >  2:2732030/1‑101 (MQ=21)
                              aaGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAgg                                                   <  2:4246633/101‑1 (MQ=21)
                              aaGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAgg                                                   <  1:6216805/101‑1 (MQ=21)
                                gATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgac                                                 >  2:6995036/1‑101 (MQ=21)
                                 aTGAATCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgacc                                                <  2:1101483/101‑1 (MQ=18)
                                  tGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccg                                               <  2:3434564/101‑1 (MQ=21)
                                  tGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccg                                               >  2:737363/1‑101 (MQ=21)
                                  tGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccg                                               >  2:1609819/1‑101 (MQ=21)
                                   gAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccga                                              >  2:412829/1‑101 (MQ=21)
                                   gAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccga                                              <  1:5858726/101‑1 (MQ=21)
                                       ccGATGTCCTTCATCTTG.CCGCAAATGTCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTc                                          <  1:3209906/101‑1 (MQ=21)
                                       ccGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTc                                          >  2:2042277/1‑101 (MQ=21)
                                        cGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         >  1:6404774/1‑101 (MQ=21)
                                        cGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         >  1:3093088/1‑101 (MQ=21)
                                         gATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        >  1:6834083/1‑101 (MQ=21)
                                         gATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        <  2:5527921/101‑1 (MQ=21)
                                         gATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        <  1:4812370/101‑1 (MQ=21)
                                          aTGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       >  1:6119807/1‑101 (MQ=21)
                                          aTGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       >  2:171199/1‑101 (MQ=21)
                                           tGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                      <  1:277050/101‑1 (MQ=21)
                                            gTCCTTCCTCTTG.CCGCAAATATCCGGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                     <  1:5836458/101‑1 (MQ=21)
                                            gTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                     >  2:930409/1‑101 (MQ=21)
                                             tCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                    <  2:6526032/101‑1 (MQ=21)
                                               cTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                  >  2:4663598/1‑101 (MQ=21)
                                               cTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                  <  1:1017520/101‑1 (MQ=21)
                                                ttCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                                 >  1:3477933/1‑101 (MQ=21)
                                                 tCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAGAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  2:5924683/1‑101 (MQ=21)
                                                 tCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:2316788/1‑101 (MQ=21)
                                                 tCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                <  1:6310672/101‑1 (MQ=21)
                                                 tCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                <  2:3908494/101‑1 (MQ=21)
                                                 tCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:4535031/1‑101 (MQ=21)
                                                  cATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               <  1:1382532/101‑1 (MQ=21)
                                                  cATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               >  1:1845856/1‑101 (MQ=21)
                                                  cATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               <  1:4359335/101‑1 (MQ=21)
                                                  cATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCACATCAGcccc                               >  2:1046768/1‑100 (MQ=21)
                                                    tCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                             >  2:1328572/1‑101 (MQ=21)
                                                    tCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                             >  2:3727454/1‑101 (MQ=21)
                                                       tG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCCGCCCGGCgtg                          >  2:486179/1‑101 (MQ=21)
                                                        g.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgagatcg                         >  2:5679336/1‑95 (MQ=21)
                                                           cGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTg                       <  2:6225871/101‑1 (MQ=25)
                                                             cAAATATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                     <  2:1803424/101‑1 (MQ=255)
                                                              aaaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCCCCCCGGCGTGTTGCAt                    >  1:5727151/1‑101 (MQ=25)
                                                              aaaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  1:2097738/1‑101 (MQ=37)
                                                              aaaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    <  2:3350145/101‑1 (MQ=255)
                                                              aaaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  2:5437342/1‑101 (MQ=37)
                                                              aaaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  2:5171300/1‑101 (MQ=37)
                                                              aaaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGGGTTGCag                    >  2:5716214/1‑100 (MQ=25)
                                                               aaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  1:3184210/1‑101 (MQ=255)
                                                               aaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  1:3525852/1‑101 (MQ=255)
                                                               aaTATCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  1:477492/1‑101 (MQ=255)
                                                                atatCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcg                  <  2:2993424/101‑1 (MQ=255)
                                                                atatCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcg                  >  2:2812664/1‑101 (MQ=255)
                                                                 tatCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgc                 <  2:1669694/101‑1 (MQ=255)
                                                                  atCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgcg                >  1:6746051/1‑101 (MQ=255)
                                                                    cccGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc              <  1:5048936/101‑1 (MQ=255)
                                                                      cGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGt            >  2:5292494/1‑101 (MQ=255)
                                                                      cGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGt            >  1:3811354/1‑101 (MQ=255)
                                                                       ggggggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  1:1304438/1‑101 (MQ=255)
                                                                       ggggggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           <  1:3924674/101‑1 (MQ=255)
                                                                        gggggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa          <  2:6050374/101‑1 (MQ=255)
                                                                        gggggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa          >  2:4039831/1‑101 (MQ=255)
                                                                        gggggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa          >  1:4021208/1‑101 (MQ=255)
                                                                         ggggAGTCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa         >  1:1537645/1‑101 (MQ=255)
                                                                          gggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAg        >  1:1978145/1‑101 (MQ=255)
                                                                           ggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgc       >  1:1754575/1‑101 (MQ=255)
                                                                            gAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  1:7067165/1‑101 (MQ=255)
                                                                            gAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  2:5978308/1‑101 (MQ=255)
                                                                                cTT.GCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc  >  2:1904300/1‑101 (MQ=255)
                                                                                                        |                                                                              
CTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTG.CCGCAAATATCCCGGGGGGAGGCTT.GCTGAAAGCAAAGACGGGGG.....GCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408396‑408571

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG