Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,801 +C 1.6% intergenic (‑97/+69) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8011.→C 1.6% 4.6 ‑1.2 ‑1.1250intergenic (‑97/+69)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (155/91):  total (159/91)
Fisher's exact test strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.53e-02

Only 100 of 360 total aligned reads displayed.

TCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGC  >  NC_014034/568701‑568901
                                                                                                     |                                                                                                     
 cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.c                                                                                                      >  2:2634546/1‑101 (MQ=255)
  aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cc                                                                                                     <  1:4004940/101‑1 (MQ=255)
  aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cc                                                                                                     >  2:3253449/1‑101 (MQ=255)
  aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cc                                                                                                     >  1:5219787/1‑101 (MQ=255)
   ttCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccc                                                                                                    >  1:287609/1‑101 (MQ=255)
    tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                   <  1:1779147/101‑1 (MQ=255)
    tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                   >  2:6544427/1‑101 (MQ=255)
        gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccc                                                                                               <  1:2822001/101‑1 (MQ=255)
        gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccc                                                                                               <  1:4399011/101‑1 (MQ=255)
          cTCCAGTTTCTTTGTGTGAACCGGGGCTTTCGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccccc                                                                                             <  2:1293182/101‑1 (MQ=255)
          cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccccc                                                                                             <  1:253691/101‑1 (MQ=255)
          cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccccc                                                                                             <  2:4162261/101‑1 (MQ=255)
           tCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccccccccc                                                                                            >  2:4422862/1‑100 (MQ=255)
            ccAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTT.GCCCGCCCCCgg                                                                                           >  2:3337483/1‑100 (MQ=255)
            ccAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccccccccca                                                                                           >  1:3441991/1‑99 (MQ=255)
            caaGTTGCTGTAGCTGAACCGGGTCTTACGCCTGTGGCTGGGGTGGGGCAATAACCCGGCCGGGCTTGGGAAGGCCCTGCGGCACCCTG.CCCCCCCCCCGa                                                                                           <  2:2481910/99‑1 (MQ=255)
             cAGTTTCTGTAGGGAAACCGGGGCTTCCCCCTGTGGCGGCGGGGGGGCAATGCCCCGCACGGGCTGGTGAAGGCGCTGCGGCATCCTC.CCCCCCCCCCGA.c                                                                                         <  2:1236741/101‑1 (MQ=255)
              aGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCGG.CCCCCCCCCCCA.Cg                                                                                        >  2:6677782/1‑101 (MQ=255)
               gTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.Cgg                                                                                       >  1:5655432/1‑101 (MQ=255)
                   cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGggg                                                                                   >  2:5265862/1‑101 (MQ=255)
                   cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCA.CCGGggg                                                                                   >  1:3005583/1‑101 (MQ=255)
                     gTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCC.CGGGggggc                                                                                 >  2:2355811/1‑101 (MQ=255)
                      tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGggggcg                                                                                >  2:4097850/1‑100 (MQ=255)
                      tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCCCCCGA.CGGGagggcag                                                                               >  2:5031877/1‑101 (MQ=255)
                        tgtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGagggcagg                                                                              <  1:3203790/101‑1 (MQ=255)
                         gtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGG.CGGGggggccggg                                                                             >  1:6439469/1‑101 (MQ=255)
                           gAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGgcggcaggggt                                                                           >  1:6035473/1‑99 (MQ=255)
                             aCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGGAGGGCGgg                                                                         >  1:3391788/1‑101 (MQ=255)
                             aCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTgg                                                                         >  1:3760390/1‑101 (MQ=255)
                              ccGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGt                                                                        >  1:2227316/1‑101 (MQ=255)
                               cGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CCGGAGGGCAGGGCTGGtt                                                                       >  2:2854452/1‑101 (MQ=255)
                               cGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTgggt                                                                       >  2:6142337/1‑99 (MQ=255)
                                ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGGTGGGGCATCCTG.CCCCCCCCCGGC.CGGGCGGGACGGGCGggggt                                                                      >  2:2284904/1‑98 (MQ=255)
                                ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGttt                                                                      <  1:6624588/101‑1 (MQ=255)
                                ggggCTTACGCCTGGGGCTGGGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGttt                                                                      <  1:6132829/101‑1 (MQ=255)
                                ggggCTTACGCCTGGGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGttt                                                                      <  1:1904407/101‑1 (MQ=255)
                                 gggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTc                                                                     >  1:6390421/1‑101 (MQ=255)
                                    cTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCACCGGGGGGGCGGGGCGGGTTTGgg                                                                   >  2:6952412/1‑101 (MQ=255)
                                     ttACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGGGGGCAGGGCTGGTTTCGggc                                                                 >  2:5532322/1‑101 (MQ=255)
                                      tACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.AGGGAGGGGAGGGCTGGGTTCggggg                                                                >  2:3990585/1‑99 (MQ=255)
                                        cGCCTGGGGCGGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGggcggc                                                              <  1:1029183/101‑1 (MQ=255)
                                         gCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGAGGGCAGGGCTGGTTTCGGGGGGCa                                                             >  2:5347112/1‑101 (MQ=255)
                                         gCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCa                                                             <  2:1683419/101‑1 (MQ=255)
                                             gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGgc                                                         <  1:4518194/101‑1 (MQ=255)
                                             gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGgc                                                         >  2:4667358/1‑101 (MQ=255)
                                              tgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGgcg                                                        >  2:1870609/1‑101 (MQ=255)
                                                gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGGGGGCAGGGCTGGGTTCGGGCGGCAAGGCGcc                                                      >  2:3478816/1‑101 (MQ=255)
                                                gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                      <  2:4838881/101‑1 (MQ=255)
                                                gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                      >  1:1651249/1‑101 (MQ=255)
                                                 cggCGGGGGGGCACTGACCCGCACGGGCTTGTGCAGGCCCTGCGGCCCCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                     <  1:2117353/99‑1 (MQ=255)
                                                    cGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcg                                                  >  1:4225502/1‑101 (MQ=255)
                                                    cGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcg                                                  >  2:2074630/1‑101 (MQ=255)
                                                     ggTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCcgcgg                                                 >  1:6876009/1‑101 (MQ=255)
                                                        ggggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgc                                              >  1:1225948/1‑101 (MQ=255)
                                                         gggCAATGCCCCGCACGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcg                                             <  2:2956018/101‑1 (MQ=255)
                                                         gggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTGCCCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcccggcgc                                              >  1:988142/1‑101 (MQ=255)
                                                          ggCAATGCCCCGCAGGGGCTTGTGAAGGCGCTGCGGCACCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcgg                                            <  1:2660321/101‑1 (MQ=255)
                                                          ggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcgg                                            >  2:5432664/1‑101 (MQ=255)
                                                          ggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcgg                                            >  2:6212049/1‑101 (MQ=255)
                                                            cAATGCCCCGCACGGGGTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                          <  2:4908992/101‑1 (MQ=255)
                                                            cAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGC.CGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGGGGcc                                          >  1:4361927/1‑101 (MQ=255)
                                                            cAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                          <  1:5909197/101‑1 (MQ=255)
                                                             aaTGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGGAAGGCGCCCGCGGCGCGGCCg                                         >  2:1206575/1‑101 (MQ=255)
                                                               tGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTg                                       >  2:1540334/1‑101 (MQ=255)
                                                                 aCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGcc                                     <  2:3598373/101‑1 (MQ=255)
                                                                  cccGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                    <  1:1542794/101‑1 (MQ=255)
                                                                   ccgctggggCTGGGGAAGGCGGTGCGGCACCCGG.CCCCGCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTg                                   <  2:5803611/95‑1 (MQ=255)
                                                                   ccGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTTGGGCGGCAAGGCGCCCGCGGCGCGGCCGCGCCTg                                   >  2:4725179/1‑101 (MQ=255)
                                                                    cGCAGGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGc                                  <  2:978887/101‑1 (MQ=255)
                                                                     gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGGCGGGCCCGCt                                 >  1:2328507/1‑101 (MQ=255)
                                                                     gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGcc                                 >  1:4969987/1‑100 (MQ=255)
                                                                      cgtgggCTGGGGAAGGCGCTGCGCCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCTGCCGTGCCTGCtt                                <  1:1392365/98‑1 (MQ=255)
                                                                      cccGGGCTTGGGAAGGCGCCGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                <  1:5035544/99‑1 (MQ=255)
                                                                      cACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                <  1:1991516/101‑1 (MQ=255)
                                                                      cACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                <  1:76640/101‑1 (MQ=255)
                                                                       cggggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                               <  2:6850598/99‑1 (MQ=255)
                                                                       aCGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCCGGGCTGGGTTCGGGGGGGAGGGCGCCCGCGGCGCGGGCGGGCGCGCttc                               >  1:3293064/1‑100 (MQ=255)
                                                                       aCGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                               <  2:5140764/101‑1 (MQ=255)
                                                                         gggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCCCCGGGGCCGCCGGGCCTggctaac                             >  2:5195176/1‑95 (MQ=255)
                                                                          gggttGTGAAGGCGCTGCGGCATCCGG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGc                            <  1:1002783/98‑1 (MQ=255)
                                                                            gttGTGAGGGCGCGGCGGCCTCCTGCCCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGcc                           <  2:2901530/100‑1 (MQ=255)
                                                                            cTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCt                          >  2:1398514/1‑101 (MQ=255)
                                                                              tgtgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGc                        >  2:5686486/1‑101 (MQ=255)
                                                                               gtgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCa                       >  1:4895833/1‑101 (MQ=255)
                                                                               gtcaaGGCGCTGAGGCATCGTC.CCCCCCCCCCGA.CCGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGTCCTTGCCTGCTTACGCCTGCa                       <  2:4486875/98‑1 (MQ=255)
                                                                                 gAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGCCGTGCCTGCTTACGCCTGGAgg                     >  1:4134869/1‑100 (MQ=255)
                                                                                 gAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGGGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGc                     >  1:5860405/1‑101 (MQ=255)
                                                                                     gcgcTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGGCGTGCCTGCTTACGCCTGCCGCgggc                 >  2:754053/1‑99 (MQ=255)
                                                                                      cgcTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCg                <  1:3110975/101‑1 (MQ=255)
                                                                                            ggCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCGGACCgg          >  1:4746484/1‑100 (MQ=255)
                                                                                             gCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCgag         <  1:924990/101‑1 (MQ=255)
                                                                                             gCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCGGACCgag         >  1:624350/1‑101 (MQ=255)
                                                                                              cctCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCgaga        <  1:6771098/99‑1 (MQ=255)
                                                                                              cATCCTG.CCCCCCCCCCGA.CGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCgaga        >  1:2019352/1‑101 (MQ=255)
                                                                                               aTCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt       <  2:1993506/101‑1 (MQ=255)
                                                                                               aTCCTG..CCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCGGaaccggatt      >  1:5392178/1‑93 (MQ=255)
                                                                                                  cTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc    <  2:1109022/101‑1 (MQ=255)
                                                                                                   tG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttttg   >  2:6464461/1‑99 (MQ=255)
                                                                                                    g.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGGCGGACCGAGATTTccc  >  2:70333/1‑99 (MQ=255)
                                                                                                    g.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGGTTTCGc  >  2:601846/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
TCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGA.CGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGC  >  NC_014034/568701‑568901

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG