Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 586,917 +G 100% intergenic (‑145/+37) RCAP_rcc00548 ← / ← purL BolA family protein/phosphoribosylformylglycinamidine synthase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034586,9171.→G100.0% 48.3 271intergenic (‑145/+37)RCAP_rcc00548/purLBolA family protein/phosphoribosylformylglycinamidine synthase II
Reads supporting (aligned to +/- strand):  new base (126/127):  ref base (2/16):  total (128/143)
Fisher's exact test strand distribution p-value = 1.22e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.70e-01

Only 100 of 325 total aligned reads displayed.

GGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGC  >  NC_014034/586817‑587011
                                                                                                      |                                                                                               
ggCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.c                                                                                                  >  2:3985456/1‑101 (MQ=255)
 gCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..g                                                                                               >  2:6973759/1‑101 (MQ=255)
   tctaaGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggg                                                                                             <  2:2211046/98‑1 (MQ=255)
   gACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggg                                                                                             <  2:3052602/101‑1 (MQ=255)
    aCAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..gggg                                                                                            <  1:4640341/101‑1 (MQ=255)
    aCAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..gggg                                                                                            >  1:3628683/1‑101 (MQ=255)
    aCAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..gggg                                                                                            >  1:3017258/1‑101 (MQ=255)
     cAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggggg                                                                                           >  2:6208473/1‑101 (MQ=255)
       agagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggggggg                                                                                         >  2:1714226/1‑101 (MQ=255)
       agagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggggggg                                                                                         >  2:4174231/1‑101 (MQ=255)
           ccAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggggggggcgc                                                                                     >  1:5538313/1‑97 (MQ=255)
           ccAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggggggggcgc                                                                                     >  2:6115013/1‑97 (MQ=255)
            cAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggggggggcgct                                                                                    >  2:5069693/1‑96 (MQ=255)
             aggcgccGGTTGGGTGTGCCAGGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCtt                                                                                    <  2:1078147/96‑1 (MQ=255)
             aaGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggggggggcgttt                                                                                   >  1:2888590/1‑95 (MQ=255)
              aGCCCCGGTTGCGTGTGCCAAGTGACGCAGCGGGGGGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTc                                                                                   <  1:6466973/101‑1 (MQ=255)
              aGCCCCGGTTGCGTGGGCCAAGTGACGGAGCGGGCAGGGGCCGCGGCGAGGGCGGGGGTCAGGGCGGGGGCAAGTGTCGCCGAGCT.GG.GGGGGGGCGCTTc                                                                                   <  1:134277/101‑1 (MQ=255)
              aGCACGGGTGGCGTGTGCCAAGTGCCGCAGCGGGCGGGGGCCGCGGCGAGGAGGGGGGGCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTc                                                                                   <  2:5146100/101‑1 (MQ=255)
               gccccGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCt                                                                                  <  1:3825557/98‑1 (MQ=255)
                cgcgggTTGCGTGGGCCAAGTGACGGAGCGGGCGGGGGCCGCGGCGGGGACGGGGGTCAGGAGGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTg                                                                                 <  2:3765140/97‑1 (MQ=255)
                cACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTg                                                                                 >  1:895502/1‑101 (MQ=255)
                cACCGGTGCCGTGGGCGAAGTGACGCGGCGGGCAGGGGCCGCGGCGGGGACGGGGGTCGGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTg                                                                                 <  2:1936318/101‑1 (MQ=255)
                 aCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgc                                                                                >  1:6951015/1‑101 (MQ=255)
                 aCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..ggggggggggctccgtc                                                                               >  2:2298354/1‑91 (MQ=255)
                   cGGTTGCGTGTGCCAAGTGACGCGGCGGGCGGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgccc                                                                              <  1:2024537/101‑1 (MQ=255)
                   cGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgccc                                                                              >  1:3589049/1‑101 (MQ=255)
                   cGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgccc                                                                              <  1:2338813/101‑1 (MQ=255)
                    ggTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgcccg                                                                             >  1:4308141/1‑101 (MQ=255)
                    ggTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGAAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgcccg                                                                             >  1:6312878/1‑101 (MQ=255)
                    ggTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGCGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGGTTCTgcccg                                                                             >  2:5874430/1‑101 (MQ=255)
                     gTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgcccgc                                                                            >  2:648853/1‑101 (MQ=255)
                     gTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgcccgc                                                                            >  2:4512004/1‑101 (MQ=255)
                       tGCGTGTGCCAAGTGGCGCAGCGGGCAGGGGCCGCGGCGAGGAGGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCTGG..GGGGGGGCGCTTCTgcccgccc                                                                          <  1:1436749/101‑1 (MQ=255)
                       tGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTgcccgccc                                                                          >  2:770255/1‑101 (MQ=255)
                        gCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGGTTCTGGCCGcccc                                                                         >  1:2213054/1‑101 (MQ=255)
                        gCGTGTGCCAAGGGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGcccc                                                                         <  2:5422050/101‑1 (MQ=255)
                          gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCgg                                                                       >  2:521994/1‑101 (MQ=255)
                            gtgCCAAGTGCGGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTGAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAg                                                                     <  1:1008808/101‑1 (MQ=255)
                             tgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGc                                                                    >  2:1417051/1‑101 (MQ=255)
                              gCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCt                                                                   >  1:1154003/1‑101 (MQ=255)
                               ccAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..GGGGGGGCGCCTCTGCCCGCCCCGGAGCTTg                                                                 >  1:724968/1‑101 (MQ=255)
                                cgaGTGCCGCGGCGGGCAGGGGCGGCGGCGAGGGCGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCTGG..GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTg                                                                 <  1:3148144/99‑1 (MQ=255)
                                cAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTg                                                                 <  2:1298867/101‑1 (MQ=255)
                                 aaGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGc                                                                >  1:599960/1‑101 (MQ=255)
                                   gTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGt                                                              <  1:4213797/101‑1 (MQ=255)
                                    tGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGtt                                                             <  2:6892054/101‑1 (MQ=255)
                                     gACGCAGCGGGCAGGGTCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGttt                                                            >  1:5123936/1‑101 (MQ=255)
                                     gACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGttt                                                            >  1:1056480/1‑101 (MQ=255)
                                      gcGCAGCGGGGAGGGGCCGCGGCGAGGGCGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGtttt                                                           <  2:1594190/100‑1 (MQ=255)
                                      aCGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGtttt                                                           >  2:2132073/1‑101 (MQ=255)
                                       gtctgCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                          <  2:862347/97‑1 (MQ=255)
                                       cgcggggggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                          <  2:2839610/95‑1 (MQ=255)
                                         cAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAg                                                        >  2:6346084/1‑101 (MQ=255)
                                         cAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAg                                                        >  2:3843089/1‑101 (MQ=255)
                                          ggCGGGCGGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGc                                                       <  1:1916823/100‑1 (MQ=255)
                                          aGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGGTTTCAGc                                                       >  1:1724115/1‑101 (MQ=255)
                                             gggggggggCGGCGGGGGGGGGGGGGGTCAGGAGGGGGGCAAGGGTCGCCGAGCTGG..GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCtt                                                    <  1:3424271/96‑1 (MQ=255)
                                              ggggggggCCGGGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTc                                                   <  1:6507836/97‑1 (MQ=255)
                                              ggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCATTc                                                   >  2:5610453/1‑101 (MQ=255)
                                               gcgggggCCGCGGGGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGGGCTGG..GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                  <  1:2826446/98‑1 (MQ=255)
                                               gCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                  <  2:4772145/101‑1 (MQ=255)
                                                ggggggCCGCGGAGAGGACGGGGGTCAGGAGGGGGGCAAGTGTCGCCGAGCTGG..GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGa                                                 <  2:267679/99‑1 (MQ=255)
                                                cAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGa                                                 >  1:2633055/1‑101 (MQ=255)
                                                  ggggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                               >  2:6268224/1‑101 (MQ=255)
                                                  ggggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                               <  2:3290339/101‑1 (MQ=255)
                                                           gcgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGgcggcggcg                                      >  2:3556429/1‑101 (MQ=255)
                                                            ggAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGa                                     <  2:2924353/100‑1 (MQ=255)
                                                            cgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CGGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGgcggcggcg                                      >  2:6603808/1‑101 (MQ=255)
                                                              aGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAg                                   >  1:3383438/1‑101 (MQ=255)
                                                               ggAGGGGGGTCAGGACGGGGGCAGGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAgg                                  <  2:5004827/101‑1 (MQ=255)
                                                                gACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgc                                 >  2:3590009/1‑101 (MQ=255)
                                                                  ggggggTCGGGGCGGGGGCAGGTGCCGCGGAGCT.G..GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggt                              <  2:724948/100‑1 (MQ=255)
                                                                  cGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcgg                               >  1:815545/1‑101 (MQ=255)
                                                                     gggTCAGGACGGGGGCAAGTGTCGCCGAGCT.CGGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtg                             >  2:1197216/1‑101 (MQ=255)
                                                                     gggTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTAGACGGCGGCGGCGAAGgcggtgc                            <  2:1026858/101‑1 (MQ=255)
                                                                      ggTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcg                           <  2:5310972/101‑1 (MQ=255)
                                                                       gTCAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcgg                          <  2:2017270/101‑1 (MQ=255)
                                                                        tCAGGGCGGGGGCAGGTGTCGCCGGGCTGG..GGGGGGGCGCTTTTGCCCGCCCCGGAGCTTGCGTTTTCAGCC.TCGACGGCGGCGGCGAAGGCGGTGCGGTa                        <  1:5775073/101‑1 (MQ=255)
                                                                         cAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                        <  1:522006/101‑1 (MQ=255)
                                                                         cAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                        >  2:6023271/1‑101 (MQ=255)
                                                                         cAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                        >  2:5487870/1‑101 (MQ=255)
                                                                         cAGGACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGGGCGGTa                        >  2:4050554/1‑101 (MQ=255)
                                                                          gggACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaa                       <  1:6258003/100‑1 (MQ=255)
                                                                           ggACGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaaa                      >  2:4731541/1‑101 (MQ=255)
                                                                              cGGGGGCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCg                   >  1:7017808/1‑101 (MQ=255)
                                                                               gggggCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGt                  >  2:2395065/1‑101 (MQ=255)
                                                                                ggggCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTg                 <  1:6115325/101‑1 (MQ=255)
                                                                                 gggCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGc                <  2:2981320/101‑1 (MQ=255)
                                                                                  ggCAAGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCt               <  2:672700/101‑1 (MQ=255)
                                                                                   gCAAGTGTCGCCGGGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTg              <  1:3542990/101‑1 (MQ=255)
                                                                                     aaGTGTCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg            <  2:7060478/101‑1 (MQ=255)
                                                                                         gtCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct        <  1:4547869/101‑1 (MQ=255)
                                                                                         gtCGCCGAGCG.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct        <  2:250955/101‑1 (MQ=255)
                                                                                         gtCCCCGGGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct        <  1:2125828/101‑1 (MQ=255)
                                                                                          tCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTg       <  1:5873156/101‑1 (MQ=255)
                                                                                          tCGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTg       <  1:117246/101‑1 (MQ=255)
                                                                                           cGCCGGGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGc      >  1:6864574/1‑101 (MQ=255)
                                                                                           cGCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGc      <  2:5731677/101‑1 (MQ=255)
                                                                                           cGCCGAGCT.CG.GGGGGGGCGCTGCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGc      <  1:2543697/101‑1 (MQ=255)
                                                                                            gCCGAGCT.CG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGcc     >  1:5586700/1‑101 (MQ=255)
                                                                                                      |                                                                                               
GGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGCT.C..GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGC  >  NC_014034/586817‑587011

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG