Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 735,233 +C 1.5% intergenic (‑139/‑346) bchF ← / → ppaA 2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034735,2331.→C 1.5% 6.6 ‑0.9 ‑0.6325intergenic (‑139/‑346)bchF/ppaA2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (182/138):  total (186/139)
Fisher's exact test strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.62e-01

Only 100 of 399 total aligned reads displayed.

TAAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCG  >  NC_014034/735133‑735333
                                                                                                                                                                                                         
tAAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCgg                                                                                                      >  1:2147321/1‑101 (MQ=255)
 aaaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGc                                                                                                     <  1:3836328/101‑1 (MQ=255)
 aaaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGc                                                                                                     >  2:5989115/1‑101 (MQ=255)
    aCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcccc                                                                                                  >  2:7013855/1‑101 (MQ=255)
       ggTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccccccc                                                                                               >  2:4188552/1‑101 (MQ=255)
       ggTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccccccc                                                                                               <  2:2532073/101‑1 (MQ=255)
       ggTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccccccc                                                                                               <  1:301190/101‑1 (MQ=255)
        gTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcccccccc                                                                                              >  2:2196340/1‑101 (MQ=255)
        gTCTTGTCACGAGAAAGTGTAAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGcccccccc                                                                                              <  1:5603230/101‑1 (MQ=255)
         gcTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACCCCGCTTCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGccccccccc                                                                                             <  1:7063223/100‑1 (MQ=255)
          cTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCg                                                                                            >  1:5445730/1‑101 (MQ=255)
           ttGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCgg                                                                                           <  2:5883392/101‑1 (MQ=255)
             gTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCt                                                                                         <  1:6728471/101‑1 (MQ=255)
             gTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCt                                                                                         >  1:4200941/1‑101 (MQ=255)
               cACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTaa                                                                                       >  2:3011395/1‑101 (MQ=255)
               cACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTaa                                                                                       <  2:7064327/101‑1 (MQ=255)
                aCGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGCCTaaa                                                                                      >  2:4469981/1‑101 (MQ=255)
                   agaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGGCCCCCCCCGGCTAAAccc                                                                                   >  2:1750301/1‑100 (MQ=255)
                     aaaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACcgcg                                                                                 >  1:6131084/1‑101 (MQ=255)
                     aaaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCACCGGCTAAACcgcg                                                                                 >  2:3888502/1‑101 (MQ=255)
                       aGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGt                                                                               >  1:4595898/1‑101 (MQ=255)
                         tgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCa                                                                             <  1:3994631/101‑1 (MQ=255)
                          gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCAc                                                                            <  1:1482715/101‑1 (MQ=255)
                             aaTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGAc                                                                         <  1:140371/101‑1 (MQ=255)
                             aaTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGAc                                                                         >  2:5589722/1‑101 (MQ=255)
                              aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACt                                                                        >  1:2208555/1‑101 (MQ=255)
                               tGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCGGCTAAACCGCGGTCACGACTCg                                                                      >  1:4664791/1‑101 (MQ=255)
                                gAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCg                                                                      >  1:1135435/1‑101 (MQ=255)
                                gAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCg                                                                      >  1:6042943/1‑101 (MQ=255)
                                 aaaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGt                                                                     <  2:3484467/101‑1 (MQ=255)
                                  aaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTc                                                                    <  2:6142999/101‑1 (MQ=255)
                                  aaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCACGGGCACGACTCggc                                                                    >  2:1220376/1‑99 (MQ=255)
                                   aaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTcc                                                                   >  2:4583989/1‑101 (MQ=255)
                                     cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCgg                                                                 >  1:5243508/1‑101 (MQ=255)
                                     cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCgg                                                                 <  1:750055/101‑1 (MQ=255)
                                     cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCgg                                                                 >  1:3775696/1‑101 (MQ=255)
                                      ttACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCCGGGCCACGACTCGCCCggc                                                                >  2:2669734/1‑100 (MQ=255)
                                      ctACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCggg                                                                <  2:5699200/100‑1 (MQ=255)
                                        acacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCa                                                              >  2:5021714/1‑101 (MQ=255)
                                         cacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAg                                                             >  1:1779268/1‑101 (MQ=255)
                                         cacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGACCCCCCCCGGCTAAACCGCGGTCAAGACTCAACCCGGCAg                                                             >  1:4320286/1‑101 (MQ=255)
                                          acTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCCGCCGGGCAGa                                                            >  2:3312291/1‑101 (MQ=255)
                                           cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           >  2:88751/1‑101 (MQ=255)
                                           cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           >  1:4882284/1‑101 (MQ=255)
                                             cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                         >  2:2785381/1‑101 (MQ=255)
                                             cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                         >  2:3553035/1‑101 (MQ=255)
                                             cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                         <  2:313144/101‑1 (MQ=255)
                                              aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAAGCGCGGCCACGACTCGCCCTGGCAAGAAcc                                                        >  2:4424678/1‑101 (MQ=255)
                                              aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                        >  2:4797239/1‑101 (MQ=255)
                                              aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAcc                                                        <  1:1188768/101‑1 (MQ=255)
                                              aGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCCGGCAGAAAcc                                                        >  2:109997/1‑101 (MQ=255)
                                                  gCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggg                                                    >  2:6640924/1‑100 (MQ=255)
                                                   cGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcg                                                   >  1:6498328/1‑101 (MQ=255)
                                                    gggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcgg                                                  >  2:4105483/1‑101 (MQ=255)
                                                     ggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcggg                                                 <  2:1390909/101‑1 (MQ=255)
                                                         gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                             >  1:2399067/1‑101 (MQ=255)
                                                         gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                             >  2:4896727/1‑101 (MQ=255)
                                                           aaCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGa                                           >  2:767082/1‑101 (MQ=255)
                                                            aCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGAt                                          <  2:1668921/101‑1 (MQ=255)
                                                             cgccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATg                                         <  1:5918521/101‑1 (MQ=255)
                                                              gccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgc                                        <  1:446547/101‑1 (MQ=255)
                                                              gccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgc                                        <  1:4959560/101‑1 (MQ=255)
                                                                cgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgcgc                                      <  2:1469960/101‑1 (MQ=255)
                                                                 gcTGCCCTTGCGTGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGAGGGAGGCGcc                                     >  1:5082513/1‑101 (MQ=255)
                                                                 gcTGCCCTTGCGCGCACGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGCAACCGGgcggggccggcgggcgc                                     >  1:3127018/1‑94 (MQ=255)
                                                                  cTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCt                                    <  1:7105197/101‑1 (MQ=255)
                                                                   tGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCtt                                   <  1:1525971/101‑1 (MQ=255)
                                                                   tGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCtt                                   >  2:4226670/1‑101 (MQ=255)
                                                                    gCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGGCttt                                  >  2:6465429/1‑101 (MQ=255)
                                                                    gCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCttt                                  <  1:1149979/101‑1 (MQ=255)
                                                                      ccTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTtc                                >  1:5699860/1‑101 (MQ=255)
                                                                       cTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtct                               <  2:692935/101‑1 (MQ=255)
                                                                       cTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtcc                               >  2:2940222/1‑100 (MQ=255)
                                                                        ttGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTtctc                              >  1:4025085/1‑101 (MQ=255)
                                                                         tGCGCGCAAGTGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCa                             <  1:6298682/101‑1 (MQ=255)
                                                                           cgcgcAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAAc                           <  2:244198/101‑1 (MQ=255)
                                                                            gcgcAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGCCTAAACCGCGGTCACGACTCGCCCGGGCGGAAACCGGGGGGGGGGGGAGGGGCCTTTTCCCAAcc                          >  1:3262878/1‑101 (MQ=255)
                                                                             cgcAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCt                         <  1:2683433/101‑1 (MQ=255)
                                                                               cAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGGCTTTTCTCAACCTgg                       >  2:4338606/1‑101 (MQ=255)
                                                                                aaGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTTCCCAACCTGGc                      >  1:1942135/1‑101 (MQ=255)
                                                                                  gggCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGccc                    <  2:6858445/101‑1 (MQ=255)
                                                                                  gggCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGccc                    >  2:3779702/1‑101 (MQ=255)
                                                                                   ggCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCg                   <  1:6879737/101‑1 (MQ=255)
                                                                                      gcCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGGGACTTTTCTTAACCTGGCCCGTAt                >  1:6585969/1‑101 (MQ=255)
                                                                                      gcCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTAt                <  1:7003609/101‑1 (MQ=255)
                                                                                       ccTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATc               <  2:1010172/101‑1 (MQ=255)
                                                                                        cTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCTTTTCCCCACCCGGGCCGGATCt              >  2:6722369/1‑101 (MQ=255)
                                                                                        cTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCt              <  2:2024234/101‑1 (MQ=255)
                                                                                          tGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTg            >  1:6627494/1‑101 (MQ=255)
                                                                                          tGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTg            >  1:3550217/1‑101 (MQ=255)
                                                                                             tGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgt         <  2:6223999/101‑1 (MQ=255)
                                                                                              gCAGCGG.CCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcg       <  1:2520518/101‑1 (MQ=255)
                                                                                               cAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcg       <  2:5219828/101‑1 (MQ=255)
                                                                                                ggCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcgt      <  1:914723/100‑1 (MQ=255)
                                                                                                aGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGGCTTTTCTCAACCTGGCCCGTATCTTGcgtcgt      >  2:5442632/1‑101 (MQ=255)
                                                                                                aGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcgt      <  2:2088673/101‑1 (MQ=255)
                                                                                                 gCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTg     >  1:4778263/1‑101 (MQ=255)
                                                                                                   ggCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGc   >  2:189048/1‑101 (MQ=255)
                                                                                                   ggCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGc   <  1:2785483/101‑1 (MQ=255)
                                                                                                    gCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCg  <  1:3435978/101‑1 (MQ=255)
                                                                                                                                                                                                         
TAAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCG  >  NC_014034/735133‑735333

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG