Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,640	+C	100%	intergenic (‑94/+26)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,640	1	.→C	100.0%	135.2	37	intergenic (‑94/+26)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (33/3):  ref base (1/0):  total (34/3)

CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GGT.C..GA.CG.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGC  >  NC_014034/774595‑774730                                                |                                                                                               cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg                                       >  2:904313/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACg                                       >  1:888017/11‑101 (MQ=255) cgcaggcccggcgcCCCCCCGCCCGGGGCGGACCGGCCCCGCC.GGTCC..GACCC.TACGGTCTCCTATCCTGCGATCACTTCGCCCCCCACCCGTCGGCCACg                                       >  1:3432203/11‑101 (MQ=255)  gcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGt                                      >  2:5550855/10‑101 (MQ=255)  gcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGt                                      >  1:1684666/10‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGTTc                                    >  2:6371066/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                    >  2:3251495/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                    >  1:4138066/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                    >  1:7027323/8‑101 (MQ=255)    aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                    >  1:688960/8‑101 (MQ=255)     ggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTcc                                   >  2:2832121/7‑101 (MQ=255)      gcccggcgcCCCCCCGCCCGGGGCGGACCGGCCCTGCCGGGC.C..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCCGTCGGCCACGGTccc                                  >  2:109810/6‑100 (MQ=255)         cggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTa                               >  1:4070306/3‑101 (MQ=255)          ggcgcCCCCCCGCCCGGGTGGGACCGGCCCTGCC.GGT.CCGGC.CC.TACGGTCTCTTGTCCTGCGATCACTTCGCCCGCGACCACGCGGCCACGATCCAGTAt                              >  2:6639767/2‑101 (MQ=255)          ggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAt                              >  1:7134034/2‑101 (MQ=255)            cgcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTc                            >  1:1015695/1‑101 (MQ=255)             gcCCCCCCGCCCGGGTCGGACCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCACTATTcc                           >  1:1654780/1‑100 (MQ=255)                      cccgggtcggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAg                  >  2:3545276/11‑101 (MQ=255)                       ccgggtcggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAgg                 <  2:7133660/92‑1 (MQ=255)                       ccgggtcggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAgg                 >  1:1730766/10‑101 (MQ=255)                        cgggtcggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc                >  1:6796145/9‑101 (MQ=255)                        cgggtcggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc                >  1:5932333/9‑101 (MQ=255)                         gggtcggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCg               >  1:6999546/8‑101 (MQ=255)                         gggtcggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCg               >  2:1724507/8‑101 (MQ=255)                            tcggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc            >  1:5309479/5‑101 (MQ=255)                             cggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  2:1521598/4‑101 (MQ=255)                             cggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:1064162/4‑101 (MQ=255)                              ggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCCGGCGTTCAt          >  2:1836604/3‑101 (MQ=255)                              ggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          >  1:4021938/3‑101 (MQ=255)                              ggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          >  2:5834067/3‑101 (MQ=255)                              ggaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          >  2:624581/3‑101 (MQ=255)                              ggaCCGCCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          <  2:1015660/99‑1 (MQ=255)                               gaCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc         >  2:6809239/2‑101 (MQ=255)                                aCCGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct        >  1:2826196/1‑101 (MQ=255)                                 ccGGCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc       <  1:4104004/101‑1 (MQ=255)                                   ggCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa     >  2:5565622/1‑101 (MQ=255)                                    gCCCTGCC.GGTCC..GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    >  1:1487437/1‑101 (MQ=255)                                      ccTGCC.GGTCC..GA.CCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGc  >  2:2908050/1‑101 (MQ=255)                                      ccTGCC.GGTCC..GA.CCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGc  >  1:5118524/1‑101 (MQ=255)                                                |                                                                                               CCGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCC.GGT.C..GA.CG.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGC  >  NC_014034/774595‑774730 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG