Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	900,802	+CC	100%	intergenic (‑131/‑156)	ldh ← / → ftsW	L‑lactate dehydrogenase/cell division protein FtsW

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	900,802	1	.→C	100.0%	201.7	86	intergenic (‑131/‑156)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (61/24):  ref base (0/1):  total (61/25)
*	NC_014034	900,802	2	.→C	100.0%	203.9	87	intergenic (‑131/‑156)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (61/25):  ref base (0/1):  total (61/26)

AACCCCGTTTCCG.CGCGG..CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGC...CG...CGGA..G.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGAG  >  NC_014034/900725‑900875                                                                                             ||                                                                             aaCCCCGTTTCCGCCGCGGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGTTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCg                                                           >  1:2856047/1‑101 (MQ=16) aaCCCCGTTTCCGCCGCGGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCg                                                           >  1:5437026/1‑101 (MQ=16)   ccccGTTTCCGCCGCGGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCTCCGGc                                                         >  2:4269828/1‑101 (MQ=16)      cttttCCG.CGCGGCCCGCCGCTTTGCCGCAGCCCCGTTTTTCCTTGGTGCAAATACGCCGGGGGGGGGGGG...GG...CGGA..GCC...CCCGCGGGGCGCCCCGGCCCt                                                      <  1:6293383/99‑1 (MQ=9)      ctttccggccgcgGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCt                                                      <  2:2623100/92‑1 (MQ=12)      cGTTTCCGCCGCGGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGcccc                                                      >  2:5825527/1‑100 (MQ=16)      cGTTGCCGCCGCGGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCt                                                      <  1:6817797/101‑1 (MQ=12)      cGTTCCCGCCGCGGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCt                                                      <  1:559006/101‑1 (MQ=12)      cGTTCCCGCCGCGGC.CGCCGCTTGGCCGCGGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCt                                                      <  1:4070045/101‑1 (MQ=12)       gTTTCCGCCGCGGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTg                                                     >  2:1479131/1‑101 (MQ=16)         ttCCGCCGCGGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGcc                                                   >  1:3147339/1‑101 (MQ=16)          tccgccgcgGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCg                                                  >  2:882130/6‑101 (MQ=16)          tccgccgcgGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGGCGCTCCGGCCCTGCCg                                                  >  1:7079879/6‑101 (MQ=16)           ccgccgcgGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCgg                                                 <  1:2190201/97‑1 (MQ=18)            ccgcggcggccgccgcTTCGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...GGGGGGGGCGGGGG...GGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCgg                                                 <  1:1853127/91‑1 (MQ=9)             gccgcgGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCgggg                                               >  1:2239862/3‑101 (MQ=18)              ccgcgGC.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGc                                              >  2:3549340/2‑101 (MQ=18)                 gcggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGa                                            >  2:3882391/6‑101 (MQ=18)                  cggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaa                                           >  1:544915/5‑101 (MQ=18)                   ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                          >  2:2347923/4‑101 (MQ=18)                   ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                          <  1:5851762/98‑1 (MQ=18)                   ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                          >  2:3171835/4‑101 (MQ=18)                   ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                          >  1:3782076/4‑101 (MQ=18)                     ccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGc                                        <  1:659956/100‑1 (MQ=21)                      cgctgcTTTGCCGCAGCCCCGGTTGTCCTTGGTGCAAATCCGCC...CGGGGGGGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGcc                                       <  2:3328059/101‑1 (MQ=16)                        ccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGcccc                                     >  2:775562/1‑101 (MQ=18)                         ctcTTTGCCGCGGCCCCGGTTTTCCTTGGTGCAAATCCGCC...CGGGGGGGCGGGGG...CGGA..GCC...CCCGCCGGTCGCCCCGGCCCTGCCGGGGCGAAAGccccc                                    <  1:3697440/99‑1 (MQ=16)                         cgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGccccc                                    <  2:998348/101‑1 (MQ=18)                         cgcTTGGCCGCAGCCCCGTTTTTCCTTGGGGCAAATCCGCC...CGGGGGTGCGGGGG...CGGACCC.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGccccc                                    <  2:4881650/101‑1 (MQ=12)                         cgcTTGGCCGCAGCCCCGGTTTTCCTTGTTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGccccc                                    <  1:4443294/101‑1 (MQ=18)                          gcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcg                                   >  2:5557613/1‑101 (MQ=18)                          cgtttGGCGCACCCCCGGTGTTCCGGGGTGCAAATCCGCC...CGGGGGTGG...GGGGCCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcg                                   <  2:309216/99‑1 (MQ=9)                            tttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGACAGCCCCcgcg                                 >  1:511116/1‑101 (MQ=18)                            tttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcg                                 >  1:6446392/1‑101 (MQ=18)                            tttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcg                                 >  2:4547465/1‑101 (MQ=18)                              tGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcgcc                               >  2:799907/1‑100 (MQ=18)                               gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCgcgcgc                              >  2:6194784/1‑101 (MQ=18)                               gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCgcgcgc                              >  2:3218316/1‑101 (MQ=18)                               gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCgcgcgc                              >  1:4850977/1‑101 (MQ=18)                               gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCgcgcgc                              >  1:3143785/1‑101 (MQ=18)                                 cGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccg                            >  1:1848645/1‑101 (MQ=18)                                 cGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccg                            >  1:2074279/1‑101 (MQ=18)                                 cGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccg                            >  1:4862569/1‑101 (MQ=18)                                  gCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccgc                           >  2:5843200/1‑101 (MQ=18)                                  gCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccgc                           >  2:3577385/1‑101 (MQ=18)                                   cAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCcgcg                          >  1:1190666/1‑101 (MQ=18)                                   cAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCcgcg                          >  1:2992321/1‑101 (MQ=18)                                    aGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCgg                         >  2:715281/1‑101 (MQ=18)                                     gCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGc                        >  1:4329805/1‑101 (MQ=18)                                     gCCCCGGTTTTCCTGGGTGCAAGTCCGCC...GGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGc                        <  2:4462669/101‑1 (MQ=12)                                     gCCCCGGTTTTCCTGGGTCCAAATACGCC...GGGGGGTGG...GG...GGGC..GGGCCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGc                        <  1:1634280/101‑1 (MQ=2)                                      ccccGGTTTTCCTGGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCTCCGCGGcc                       <  2:5084591/101‑1 (MQ=17)                                       ccgggTTTCCCTGGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGccc                      <  2:3143672/98‑1 (MQ=17)                                       cccGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGccc                      >  2:5967817/1‑101 (MQ=18)                                       cccGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGACAAAGCCCCCGCGCGCCGCGGccc                      >  2:490492/1‑101 (MQ=18)                                        ccGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCg                     >  1:1363075/1‑101 (MQ=18)                                          ggTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc                   <  2:6149993/101‑1 (MQ=18)                                          ggTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc                   >  2:3185358/1‑101 (MQ=18)                                           gTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa                  <  1:4436424/101‑1 (MQ=18)                                            ttttCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaa                 >  2:5791778/1‑101 (MQ=18)                                            ttttCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaa                 >  1:4684553/1‑101 (MQ=18)                                            ttttCCTTGGTGCAAATACGCC...CGGGGGGGCGGGGG...CGGA..GCC...CCCGCGGGCCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaa                 >  1:5804958/1‑101 (MQ=21)                                              ttCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaaa               >  2:4374049/1‑101 (MQ=18)                                              ttCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaaa               >  1:7117962/1‑101 (MQ=18)                                               tCCTTGGTGCAAATGCGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg              <  1:5499016/101‑1 (MQ=18)                                               tCCTTGGTGCAAATCCGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg              <  1:4158396/101‑1 (MQ=18)                                               tCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg              <  2:445700/101‑1 (MQ=18)                                               tCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg              >  1:1307250/1‑101 (MQ=18)                                               tCCTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg              >  2:4935341/1‑101 (MQ=18)                                                ccTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCCAAAGc             <  2:6446062/101‑1 (MQ=18)                                                ccTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGc             >  1:3748615/1‑101 (MQ=18)                                                 cTTGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGct            >  1:5817736/1‑101 (MQ=18)                                                  ttGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctc           >  2:2124779/1‑101 (MQ=18)                                                  ttGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctc           >  1:63645/1‑101 (MQ=18)                                                   tGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctct          >  1:6956855/1‑101 (MQ=18)                                                   tGGTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctct          >  2:4971111/1‑101 (MQ=18)                                                     gTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTgg        >  2:5618877/1‑101 (MQ=18)                                                     gTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTgg        >  2:5487546/1‑101 (MQ=18)                                                     gTGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTgg        >  2:2027548/1‑101 (MQ=18)                                                      tGCAAATCCGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGt       <  2:2074199/101‑1 (MQ=18)                                                      tGCAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGt       >  1:4164999/1‑101 (MQ=18)                                                        cAAATACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCa     >  1:5131320/1‑101 (MQ=18)                                                         aaaTACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCCGGTCag    >  1:1288539/1‑101 (MQ=18)                                                          aaTACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAACGCTCTGGTCaga   >  1:7087496/1‑101 (MQ=18)                                                          aaTACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCaga   <  1:2860570/101‑1 (MQ=18)                                                          aaTACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCaga   <  2:6392420/101‑1 (MQ=18)                                                          aaTACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCaga   >  2:741643/1‑101 (MQ=18)                                                           aTACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCagag  >  1:1452729/1‑101 (MQ=18)                                                           aTACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCagag  >  1:6977716/1‑101 (MQ=18)                                                           aTACGCC...CGGGGGTGCGGGGG...CGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCagag  >  2:4319489/1‑101 (MQ=18)                                                                                             ||                                                                             AACCCCGTTTCCG.CGCGG..CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGC...CG...CGGA..G.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGAG  >  NC_014034/900725‑900875 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG