Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 925,909 +C 92.9% intergenic (+162/+12) RCAP_rcc00838 → / ← glcB DnaJ domain‑containing protein/malate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034925,9091.→C92.9% 57.0 ‑1.4 ‑1.4297intergenic (+162/+12)RCAP_rcc00838/glcBDnaJ domain‑containing protein/malate synthase
Reads supporting (aligned to +/- strand):  new base (158/118):  ref base (9/12):  total (167/130)
Fisher's exact test strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.55e-02

Only 100 of 337 total aligned reads displayed.

ACCCCCCGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGAA  >  NC_014034/925809‑926004
                                                                                                     |                                                                                               
cccccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAgcg                                                                                                  <  2:317976/100‑1 (MQ=255)
cccccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAgcg                                                                                                  <  1:6518461/100‑1 (MQ=255)
aCCCCCCGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAgcg                                                                                                  >  1:2692973/1‑101 (MQ=255)
   ccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.ccc                                                                                              >  1:274561/1‑101 (MQ=255)
   ccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.ccc                                                                                              <  2:4164696/101‑1 (MQ=255)
     ccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.ccccc                                                                                            >  2:5069798/1‑101 (MQ=255)
      cGGGATCTTGGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.cccccc                                                                                           <  2:207846/101‑1 (MQ=255)
      cGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.cccccc                                                                                           >  2:1764342/1‑101 (MQ=255)
      cGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.cccccc                                                                                           <  2:598004/101‑1 (MQ=255)
       gggATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.ccccccc                                                                                          <  1:1949119/101‑1 (MQ=255)
        ggATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.cccccccc                                                                                         >  1:749272/1‑100 (MQ=255)
         gATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.cccccccct                                                                                        <  1:902592/101‑3 (MQ=255)
           tatTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.CCCCCCCcttt                                                                                      <  1:2352419/101‑2 (MQ=255)
            atTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.CCCCCCCctttc                                                                                     >  1:3408136/1‑99 (MQ=255)
            atTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.CCCCCCCctttc                                                                                     <  1:4886017/101‑3 (MQ=255)
            atTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.CCCCCCCctttc                                                                                     <  1:2997926/101‑3 (MQ=255)
             tttGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCt                                                                                     <  2:6056536/101‑1 (MQ=255)
             tttGGGGCAAGGTAAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCt                                                                                     <  2:1078665/101‑1 (MQ=255)
             tttGGGCCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCt                                                                                     >  1:369342/1‑101 (MQ=255)
               tGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTa                                                                                   <  2:7135361/101‑1 (MQ=255)
                ggggCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTAt                                                                                  <  1:2768805/101‑1 (MQ=255)
                  ggCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGc                                                                                <  1:4096261/101‑1 (MQ=255)
                      aGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTg                                                                            <  2:4387862/101‑1 (MQ=255)
                      aGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTg                                                                            <  1:6727385/101‑1 (MQ=255)
                      aGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATAACCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTg                                                                            <  2:4036486/101‑1 (MQ=255)
                        gTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTggc                                                                          <  2:2132310/101‑1 (MQ=255)
                         tGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTggcg                                                                         >  1:3519101/1‑101 (MQ=255)
                         tGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTggcg                                                                         >  1:6060817/1‑101 (MQ=255)
                            aaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGcc                                                                      <  1:3865652/101‑1 (MQ=255)
                            aaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGcc                                                                      >  2:1569834/1‑101 (MQ=255)
                            aaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGcc                                                                      <  1:1513808/101‑1 (MQ=255)
                            aaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGcc                                                                      >  1:1106595/1‑101 (MQ=255)
                              gCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCtt                                                                    <  2:229092/101‑1 (MQ=255)
                               catgCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTc                                                                   <  2:6288630/98‑1 (MQ=255)
                               cAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTc                                                                   <  1:4132801/101‑1 (MQ=255)
                               cAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTc                                                                   <  1:5168991/101‑1 (MQ=255)
                                 gtcttcctcgCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAc                                                                 >  2:3486917/10‑101 (MQ=18)
                                 ggCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAc                                                                 <  1:6964506/101‑1 (MQ=255)
                                  gCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCAcc                                                                >  1:6901675/1‑101 (MQ=255)
                                   ccGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCt                                                               >  2:3847099/1‑101 (MQ=255)
                                    cGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTg                                                              <  2:6736495/101‑1 (MQ=255)
                                      cTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTgcg                                                            >  1:6513974/1‑101 (MQ=255)
                                       tttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTgcgc                                                           <  1:6007982/101‑1 (MQ=255)
                                       tttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTgcgc                                                           >  1:4497318/1‑101 (MQ=255)
                                        ttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGcc                                                          >  2:1395372/1‑101 (MQ=255)
                                        ttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGcc                                                          <  2:1312129/101‑1 (MQ=255)
                                        ttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGcc                                                          >  2:6219292/1‑101 (MQ=255)
                                         tGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGccc                                                         >  2:270993/1‑101 (MQ=255)
                                         tGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGccc                                                         <  1:6147443/101‑1 (MQ=255)
                                          tcccTTACATTTTGCCCCAAATATTCTGGGGGGAGTCCGCTTGCGGACTGTGGGCTCCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGAGCCCg                                                        <  1:1207130/100‑1 (MQ=21)
                                          gCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCg                                                        >  1:5241410/1‑101 (MQ=255)
                                          gCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCg                                                        <  2:4534899/101‑1 (MQ=255)
                                           cccTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCgg                                                       >  1:5458310/1‑101 (MQ=255)
                                            ccTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgc                                                      >  2:368974/1‑101 (MQ=255)
                                             cTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgcg                                                     >  2:775519/1‑101 (MQ=255)
                                             cTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGACCGgcg                                                     >  2:5761592/1‑101 (MQ=255)
                                              tttCATCTTGCCCCAAAAATCCCGGGGGGGGTCCGCTTGCGGACGGGGGGCAGGGCCCCCCCCTTTTTTTTGCTTTGGGGGCCTTCACCTTCGCCCGgccc                                                    >  2:5234226/1‑99 (MQ=18)
                                                tCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTg                                                  <  2:2878317/101‑1 (MQ=255)
                                                tCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTg                                                  >  2:4926585/1‑101 (MQ=255)
                                                  aTCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTggg                                                >  1:5338881/1‑100 (MQ=255)
                                                  aTCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGt                                                >  1:2031079/1‑101 (MQ=255)
                                                   tCTTGCCCAAAATTGCCCGGGGGGATTCCGCTTGCGGACGGGGTCCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTg                                               <  2:1862862/101‑1 (MQ=25)
                                                    cTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgc                                              <  1:3169707/101‑1 (MQ=255)
                                                      tGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgcag                                            >  2:2529568/1‑101 (MQ=255)
                                                           cAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGGGGCCTTCACCTGCCCCCGGCGCTGGTGCAGCACCg                                       >  1:3945578/1‑101 (MQ=25)
                                                           cAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCg                                       >  2:3889856/1‑101 (MQ=255)
                                                            aaaTATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCgg                                      >  2:1708600/1‑101 (MQ=255)
                                                             aaTATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGc                                     >  1:2432729/1‑101 (MQ=255)
                                                             aaTATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGc                                     <  2:1963328/101‑1 (MQ=255)
                                                              atatCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCt                                    <  1:1814853/101‑1 (MQ=255)
                                                               tatCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTc                                   >  2:4796671/1‑101 (MQ=255)
                                                                atCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCg                                  >  2:3050332/1‑101 (MQ=255)
                                                                 tCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCgg                                 >  1:1006557/1‑101 (MQ=255)
                                                                  cccGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCggc                                >  2:6523326/1‑100 (MQ=255)
                                                                  cccGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGt                                <  1:5823772/101‑1 (MQ=255)
                                                                   cgggggggAGCCGGCTGGCGGACGGGGGGCACCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGta                               <  1:1674521/99‑1 (MQ=37)
                                                                   ccGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCggga                               >  1:4830101/1‑99 (MQ=255)
                                                                     ggggggAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtata                             <  2:639515/101‑1 (MQ=255)
                                                                      gggggAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAg                            >  1:4764783/1‑101 (MQ=255)
                                                                       ggggAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGCATAgt                           >  2:2223629/1‑100 (MQ=255)
                                                                        gggAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGcc                          <  1:6365476/101‑1 (MQ=255)
                                                                         ggAGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGccc                         <  1:2546324/101‑1 (MQ=255)
                                                                           aGTCCGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGa                       <  2:5296006/101‑1 (MQ=255)
                                                                              ccGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGCATAGCCCCAGGa                    >  1:446358/1‑101 (MQ=37)
                                                                               cGCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGAc                   <  1:576903/101‑1 (MQ=255)
                                                                                gCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACt                  <  1:520299/101‑1 (MQ=255)
                                                                                gCTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTCGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACt                  >  2:3460325/1‑101 (MQ=255)
                                                                                 cTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTg                 >  1:1871880/1‑101 (MQ=255)
                                                                                 cTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTg                 >  1:4280007/1‑101 (MQ=255)
                                                                                 cTTGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGGATAGCCCGAGGACTg                 >  2:6647970/1‑101 (MQ=255)
                                                                                  ttGCGGACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGGATAGCCCGAGGACCgg                >  1:1455171/1‑101 (MQ=38)
                                                                                      ggACGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGGATAGCCCGAGGACTGGATcc            >  2:2441743/1‑101 (MQ=255)
                                                                                         cGGGGGGCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCggg         >  2:2393102/1‑101 (MQ=255)
                                                                                            ggggCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGGATAGCCCGAGGACTGGATCCCGGCGa      >  1:5669862/1‑101 (MQ=255)
                                                                                             gggCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAg     >  1:6253548/1‑101 (MQ=255)
                                                                                              ggCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAgg    <  1:6075086/101‑1 (MQ=255)
                                                                                               gCAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGa   >  1:3486003/1‑101 (MQ=255)
                                                                                                cAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGaa  <  2:5153919/101‑1 (MQ=255)
                                                                                                cAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGaa  <  2:5567932/101‑1 (MQ=255)
                                                                                                cAGCGCCCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGaa  >  1:2200748/1‑101 (MQ=255)
                                                                                                     |                                                                                               
ACCCCCCGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCAGCG.CCCCCCCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGAA  >  NC_014034/925809‑926004

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG