Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	974,265	+C	100%	intergenic (+145/+114)	RCAP_rcc00884 → / ← RCAP_rcc00885	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	974,265	1	.→C	100.0%	130.3	37	intergenic (+145/+114)	RCAP_rcc00884/RCAP_rcc00885	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/18):  ref base (0/0):  total (19/18)

ACGCTGTCGCGTGGCGACCCGCCG.......TCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCG.TGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAA  >  NC_014034/974175‑974353                                                                                                   |                                                                                             aCGCTGTCGCGTGGCGACCCGCCGGGGGGCTTCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCgctg                                                                                            >  2:3862324/1‑98 (MQ=18)                       cggggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCtt                                                                      <  2:5720147/92‑1 (MQ=25)                        ggggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTTCCttt                                                                     >  1:314418/9‑100 (MQ=25)                        ggggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTg                                                                     <  1:4930154/93‑1 (MQ=37)                        ggggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGGTTTTACCttt                                                                     >  1:3071955/9‑100 (MQ=25)                         gggggcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGc                                                                    <  1:2427360/94‑1 (MQ=37)                             gcttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACa                                                                <  1:5001614/98‑1 (MQ=255)                               ttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCCCCTTGCGGCaaa                                                              >  1:3233851/2‑101 (MQ=38)                               ttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTTTGACaaa                                                              >  1:3109486/2‑101 (MQ=38)                               ttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACaaa                                                              <  1:3319775/100‑1 (MQ=255)                               ttCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGGTTTTACCTTGCGACaaa                                                              >  1:3994729/2‑101 (MQ=38)                                  gCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTTCGCCAAacat                                                           >  1:1176731/1‑101 (MQ=38)                                  gCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAatat                                                           <  2:1996968/101‑1 (MQ=255)                                  gCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAaaat                                                           >  2:2576168/1‑101 (MQ=255)                                   ccccccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTCCCTTGAGACAAATATc                                                          >  2:1640679/1‑101 (MQ=38)                                    cccccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAatatac                                                         >  1:5115881/1‑99 (MQ=255)                                     ccccGGCCCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATccc                                                        <  1:1219392/101‑1 (MQ=255)                                     ccccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATccc                                                        <  1:2389467/101‑1 (MQ=255)                                      cccGGACCCCCCAGCGTATTTGCACCAAGAAACAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.g                                                      <  1:4739259/101‑1 (MQ=255)                                      cccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.g                                                      <  1:547310/101‑1 (MQ=255)                                       tctgACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gg                                                     <  1:2690867/98‑1 (MQ=255)                                       ccGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATccccg                                                      >  1:2497111/1‑99 (MQ=255)                                         ggACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.cggg                                                   >  1:2327845/1‑101 (MQ=255)                                          gACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTTACCTTGCGACAAAAATACC.agggg                                                  >  2:4284499/1‑100 (MQ=38)                                          gACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggga                                                  >  2:2690771/1‑100 (MQ=255)                                           aCCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTTCGACAAATATCCC.gggggg                                                 >  2:1405557/1‑99 (MQ=255)                                           aCCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATTCC.gggggg                                                 >  2:2046590/1‑99 (MQ=255)                                           aCCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggg                                                 >  2:5998656/1‑99 (MQ=255)                                             cccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGTGACAAATATACC.gggggacc                                               >  2:3033084/1‑97 (MQ=38)                                             cccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtcc                                               <  2:1084199/101‑5 (MQ=255)                                             cccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtcc                                               >  1:3648991/1‑97 (MQ=255)                                             cccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.ggggggcn                                               >  1:3910510/1‑97 (MQ=255)                                              ccccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccg                                              <  1:758928/101‑6 (MQ=255)                                                ccAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.cgggggccggg                                            >  2:6348916/1‑94 (MQ=255)                                                 cAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccgggg                                           <  2:4097199/101‑9 (MQ=38)                                                 cAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccgggg                                           <  1:3862487/101‑9 (MQ=38)                                                  aGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.gggggtccggggg                                          <  2:6075685/101‑10 (MQ=38)                                                         ttGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAAATTCCC.GGGG...GTGCg.ggggcggggac                               >  2:5302021/1‑91 (MQ=255)                                                                                    gacccGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCCGGGGGTCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGCGACAGCCg         <  1:5370325/98‑1 (MQ=9)                                                                                    gATCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGCGACAGCCg         <  2:3233735/101‑1 (MQ=14)                                                                                     gtCCGGCATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGa        <  2:2186351/100‑1 (MQ=2)                                                                                           cATGGCCGCTGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGaa  <  2:1176683/101‑1 (MQ=11)                                                                                                   |                                                                                             ACGCTGTCGCGTGGCGACCCGCCG.......TCGCCCCCCGGACCCCCCAGCGTATTTGCACCAAGAAAAAGCAGACGGGCGGGATCCGGCATGGCCG.TGCGCAGCGGCTTGTTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAA  >  NC_014034/974175‑974353 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG