Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,354,5820C→A 1.1% 0.5 0.0 ‑0.1443F515L (TTC→TTA) RCAP_rcc01274RAMP family CRISPR‑associated protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (219/219):  total (222/221)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.60e-01

Only 100 of 464 total aligned reads displayed.

TGGTGTGGCATGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCTTGCAATCA  >  NC_014034/1354482‑1354682
                                                                                                    |                                                                                                    
tGGTGTGGCATGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGNAGATCGGAGATACCTTc                                                                                                      >  2:1880710/1‑101 (MQ=255)
tGGTGTGGCATGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTc                                                                                                      >  1:4770047/1‑101 (MQ=255)
    gtgGCATGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCa                                                                                                  <  1:699012/101‑1 (MQ=255)
    gtgGCATGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCa                                                                                                  <  1:2424322/101‑1 (MQ=255)
     tgGCATGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCAt                                                                                                 <  1:780600/101‑1 (MQ=255)
      ggCATGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCAtt                                                                                                >  2:1719746/1‑101 (MQ=255)
          tGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCg                                                                                            <  2:1421112/101‑1 (MQ=255)
            tCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGt                                                                                          >  1:6311802/1‑101 (MQ=255)
            tCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGt                                                                                          <  1:4909497/101‑1 (MQ=255)
              gAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTAGCCATTCCCGGTTc                                                                                        >  2:2602774/1‑101 (MQ=255)
               aaaCCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCt                                                                                       <  1:2529389/101‑1 (MQ=255)
                aaCCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTa                                                                                      <  1:933845/101‑1 (MQ=255)
                 aCCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTAc                                                                                     <  2:3716669/101‑1 (MQ=255)
                   cccGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGa                                                                                   <  2:5917641/101‑1 (MQ=255)
                      ggTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCa                                                                                >  1:5248082/1‑101 (MQ=255)
                      ggTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCa                                                                                <  2:2145058/101‑1 (MQ=255)
                      ggTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCa                                                                                <  1:558372/101‑1 (MQ=255)
                       gTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCaa                                                                               >  1:1970111/1‑101 (MQ=255)
                         tCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAgg                                                                             >  1:1384700/1‑101 (MQ=255)
                           tGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGc                                                                           >  2:5157111/1‑101 (MQ=255)
                           tGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGc                                                                           <  1:1604629/101‑1 (MQ=255)
                            ggTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCa                                                                          >  2:1013807/1‑101 (MQ=255)
                            ggTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCa                                                                          >  2:1069470/1‑101 (MQ=255)
                              tCGCGGCGCAGACGCATGACCATACCAAAGCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATg                                                                        <  1:4509015/101‑1 (MQ=255)
                                tcggcgCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGtt                                                                      <  2:6468331/100‑1 (MQ=255)
                                 cggcgCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTAGCCATTCCCGGTTCTACGATCAAGGGCATGTTg                                                                     >  2:6060807/1‑101 (MQ=255)
                                   gcgcAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTgcg                                                                   >  1:6174158/1‑101 (MQ=255)
                                     gcAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTgcgcg                                                                 >  2:4315038/1‑101 (MQ=255)
                                      cAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTgcgcgc                                                                >  1:2805080/1‑101 (MQ=255)
                                        gACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCgg                                                              <  2:6272176/101‑1 (MQ=255)
                                            cATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACt                                                          >  1:2221718/1‑101 (MQ=255)
                                              tGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTgg                                                        >  2:4561839/1‑101 (MQ=255)
                                               gACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGa                                                       <  1:3096438/101‑1 (MQ=255)
                                               gACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGa                                                       >  1:1147105/1‑101 (MQ=255)
                                                aCCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTggcg                                                      >  2:4181709/1‑99 (MQ=255)
                                                aCCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAg                                                      <  1:3383509/101‑1 (MQ=255)
                                                   aTACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAgcgc                                                   >  2:3783233/1‑101 (MQ=255)
                                                   aTACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAgcgc                                                   >  2:3127337/1‑101 (MQ=255)
                                                    tACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGcc                                                  >  1:3905402/1‑101 (MQ=255)
                                                     aCCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCg                                                 <  2:5362495/101‑1 (MQ=255)
                                                      ccAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCgg                                                >  1:2892649/1‑101 (MQ=255)
                                                      ccAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCgg                                                >  1:2606538/1‑101 (MQ=255)
                                                        aaaCCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGt                                              <  1:1275559/101‑1 (MQ=255)
                                                        aaaCCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGt                                              <  2:1970039/101‑1 (MQ=255)
                                                        aaaCCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGt                                              <  2:668717/101‑1 (MQ=255)
                                                         aaCCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTa                                             >  2:2921494/1‑101 (MQ=255)
                                                         aaCCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTa                                             >  2:3280518/1‑101 (MQ=255)
                                                          aCCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTaa                                            >  1:624833/1‑101 (MQ=255)
                                                          aCCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTaa                                            <  1:3242694/101‑1 (MQ=255)
                                                            cGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAAt                                          <  1:1546794/101‑1 (MQ=255)
                                                             gCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATg                                         >  2:6512163/1‑101 (MQ=255)
                                                             gCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATg                                         <  2:230093/101‑1 (MQ=255)
                                                               ccAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGcc                                       <  1:596063/101‑1 (MQ=255)
                                                               ccAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGcc                                       <  2:4068121/101‑1 (MQ=255)
                                                                cAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTCAATGccc                                      >  2:3559502/1‑101 (MQ=255)
                                                                cAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGccc                                      >  2:1632180/1‑101 (MQ=255)
                                                                cAAGGATGTGCCTTTCCAGAAGATCGGAGATAACTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGccc                                      <  2:3896386/101‑1 (MQ=255)
                                                                  tggATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCcgc                                    <  1:1732291/100‑1 (MQ=255)
                                                                  aGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCcgc                                    >  1:2709307/1‑101 (MQ=255)
                                                                      tgtgCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCt                                >  2:4603920/1‑101 (MQ=255)
                                                                      tgtgCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCt                                <  1:2659047/101‑1 (MQ=255)
                                                                        tgCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCtat                              <  1:1451495/101‑1 (MQ=255)
                                                                        tgCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCtaa                              >  2:623179/1‑100 (MQ=255)
                                                                         gCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCtata                             <  2:13105/101‑1 (MQ=255)
                                                                         gCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCtata                             <  2:5538020/101‑1 (MQ=255)
                                                                         gCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCtata                             <  2:2333916/101‑1 (MQ=255)
                                                                            tttCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGaa                          <  1:494791/101‑1 (MQ=255)
                                                                              tCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAAtt                        <  1:4315260/101‑1 (MQ=255)
                                                                              tCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAAtt                        <  1:6491687/101‑1 (MQ=255)
                                                                               ccAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTa                       >  1:6772457/1‑101 (MQ=255)
                                                                               ccAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTa                       >  1:6662380/1‑101 (MQ=255)
                                                                                cAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCGAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTAt                      >  2:3439885/1‑101 (MQ=255)
                                                                                cAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTAt                      >  2:819503/1‑101 (MQ=255)
                                                                                cAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTAt                      <  2:2680419/101‑1 (MQ=255)
                                                                                cAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTAt                      >  1:4599273/1‑101 (MQ=255)
                                                                                 agaagaTCGGAGATGCCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATc                     <  2:6830897/101‑1 (MQ=255)
                                                                                 agaagaTCGGAGATACCTTCGCCATTCCCGGTTGTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATc                     >  1:3705708/1‑101 (MQ=255)
                                                                                 agaagaTCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATc                     >  1:5921967/1‑101 (MQ=255)
                                                                                 agaagaTCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATc                     <  1:316047/101‑1 (MQ=255)
                                                                                  gaaggTCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCg                    <  2:5633234/101‑1 (MQ=255)
                                                                                  gaagaTCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCg                    >  2:4929226/1‑101 (MQ=255)
                                                                                    agaTCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGaa                  <  2:2789723/101‑1 (MQ=255)
                                                                                     gaTCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGaaa                 <  1:1886427/101‑1 (MQ=255)
                                                                                     gaTCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGaaa                 >  2:5789514/1‑101 (MQ=255)
                                                                                      aTCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGaaaa                >  1:5371321/1‑101 (MQ=255)
                                                                                        cGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAAcc              <  2:1495420/101‑1 (MQ=255)
                                                                                        cGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAAcc              >  1:5650655/1‑101 (MQ=255)
                                                                                         ggAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCt             <  1:2115417/101‑1 (MQ=255)
                                                                                          gagaTACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTg            >  1:3441209/1‑101 (MQ=255)
                                                                                            gaTACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCt          >  1:5739578/1‑101 (MQ=255)
                                                                                            gaTACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCt          <  2:303307/101‑1 (MQ=255)
                                                                                            gaTACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGAGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCt          >  1:5100273/1‑101 (MQ=255)
                                                                                             aTACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCC.CGCCTAAAGAATTATCGAAAACCCGCtta        >  1:1043384/1‑100 (MQ=255)
                                                                                             aTACCTTAGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCtt         <  1:4143515/101‑1 (MQ=255)
                                                                                              tgccTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCTTg        <  2:7024546/99‑1 (MQ=255)
                                                                                              tACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCTTg        <  2:484638/101‑1 (MQ=255)
                                                                                                ccTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCTTGCa      >  2:1367978/1‑101 (MQ=255)
                                                                                                  ttCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCTTGCAAt    <  1:2351355/101‑1 (MQ=255)
                                                                                                   tCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCTTGCAATc   <  2:495960/101‑1 (MQ=255)
                                                                                                   tCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCTTGCAATc   <  1:2093047/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
TGGTGTGGCATGTCGAAACCCCGGTTCTGGTCGCGGCGCAGACGCATGACCATACCAAACCGCCCAAGGATGTGCCTTTCCAGAAGATCGGAGATACCTTCGCCATTCCCGGTTCTACGATCAAGGGCATGTTGCGCGCGGAACTGGAGCGCCGGGTAAATGCCCGCGCCTATAGAATTATCGAAAACCTGCTTGCAATCA  >  NC_014034/1354482‑1354682

Base quality scores:  ATCG  < 3 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG