Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,398,0312.→C 0.8% 0.5 0.0 ‑0.3398intergenic (+335/+1563)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (194/201):  total (195/203)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.61e-01

Only 100 of 443 total aligned reads displayed.

AAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATT  >  NC_014034/1397931‑1398131
                                                                                                                                                                                                          
aaaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCgg                                                                                                      <  2:3129089/101‑1 (MQ=255)
aaaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCgg                                                                                                      >  2:3200524/1‑101 (MQ=255)
aaaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCgg                                                                                                      >  1:1646685/1‑101 (MQ=255)
 aaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGa                                                                                                     <  2:3838261/101‑1 (MQ=255)
 aaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGa                                                                                                     <  1:7058060/101‑1 (MQ=255)
 aaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGa                                                                                                     <  1:794816/101‑1 (MQ=255)
  aCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAg                                                                                                    >  2:5062746/1‑101 (MQ=255)
     ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCaa                                                                                                 >  2:2141517/1‑101 (MQ=255)
      tATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGCGCAAg                                                                                                >  2:411119/1‑101 (MQ=255)
      tATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAg                                                                                                <  2:4588024/101‑1 (MQ=255)
       aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAgg                                                                                               >  2:472736/1‑100 (MQ=255)
       aTGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGt                                                                                               <  1:2623861/101‑1 (MQ=255)
             ggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTcc                                                                                         <  1:2833264/101‑1 (MQ=255)
               ctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTcccc                                                                                       <  2:2729769/101‑1 (MQ=255)
                tagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTccccc                                                                                      >  1:4951659/1‑101 (MQ=255)
                 agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTcccccc                                                                                     <  2:4272375/101‑1 (MQ=255)
                 agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTcccccc                                                                                     <  1:6275820/101‑1 (MQ=255)
                   ggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCcgc                                                                                   >  1:4047740/1‑101 (MQ=255)
                   ggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCcgc                                                                                   >  2:1490541/1‑101 (MQ=255)
                     aggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTcccccagatc                                                                                 >  1:1477791/1‑96 (MQ=255)
                      ggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGACCGCCACAGAAGCGGAGCAAGTCGCTCCCCCCCCgg                                                                                 >  1:6937616/1‑101 (MQ=37)
                       gcttCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGACACCGCCGGGCAACAAGTCCGCCA.AGAAGGCGAGCAAGTCACTCTCCCCGCggac                                                                               >  2:258344/1‑99 (MQ=25)
                       gctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCgggg                                                                               <  1:6042751/101‑1 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                                             <  1:5984033/101‑1 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                                             >  1:1407642/1‑101 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                                             <  2:6300342/101‑1 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                                             <  2:1782344/101‑1 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGc                                                                             >  2:5450639/1‑101 (MQ=255)
                           cGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGt                                                                           >  2:2331354/1‑101 (MQ=255)
                           cGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGt                                                                           <  1:5655982/101‑1 (MQ=255)
                            gCACCTTCAAAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                          <  2:6724355/101‑1 (MQ=255)
                             cACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:4342797/101‑1 (MQ=255)
                              aCCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                        >  2:6977077/1‑101 (MQ=255)
                                 ttCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  2:6990707/1‑101 (MQ=255)
                                  tCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGt                                                                    <  2:5666069/101‑1 (MQ=255)
                                    aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                                                  >  1:2455946/1‑101 (MQ=255)
                                    aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                                                  >  2:4851225/1‑101 (MQ=255)
                                    aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                                                  <  1:4851469/101‑1 (MQ=255)
                                     aaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAg                                                                 >  2:361438/1‑101 (MQ=255)
                                      aaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCAGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGc                                                                >  2:755113/1‑101 (MQ=255)
                                       aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCg                                                               >  1:5044948/1‑101 (MQ=255)
                                       aGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCg                                                               <  1:6578700/101‑1 (MQ=255)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGc                                                           >  1:6121119/1‑101 (MQ=255)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGc                                                           <  2:5733293/101‑1 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                         >  1:5210063/1‑101 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                         >  2:1130848/1‑101 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                                                        >  1:5151612/1‑101 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                                                        <  2:4018765/101‑1 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                                                        <  2:4404645/101‑1 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                                                        >  2:5317729/1‑101 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCg                                                       <  1:333622/101‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGccc                                                   <  2:849976/101‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGccc                                                   <  1:3503197/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                <  2:5182058/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaaa                                             <  2:2455852/101‑1 (MQ=255)
                                                          gCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAc                                            <  2:4664298/101‑1 (MQ=255)
                                                           cGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcc                                           >  2:5372326/1‑101 (MQ=255)
                                                           cGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGGGCATCGGCCCGATAAAcc                                           >  2:5624580/1‑101 (MQ=255)
                                                            gTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAccc                                          >  1:5587454/1‑101 (MQ=255)
                                                             tGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGCTACAaccc                                         >  2:3019870/1‑101 (MQ=255)
                                                             tGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcccc                                         >  2:3340616/1‑101 (MQ=255)
                                                              ggATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                        >  2:1375109/1‑101 (MQ=255)
                                                               caTCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGa                                       <  1:3150190/100‑1 (MQ=255)
                                                                accGACACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAt                                      <  1:2200591/99‑1 (MQ=255)
                                                                aTCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAt                                      <  1:6694534/101‑1 (MQ=255)
                                                                 tCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtt                                     >  2:3707593/1‑101 (MQ=255)
                                                                  cGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAttt                                    <  1:361442/101‑1 (MQ=255)
                                                                   gAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt                                   <  1:4715500/101‑1 (MQ=255)
                                                                     aaCCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                 <  2:6837605/101‑1 (MQ=255)
                                                                     aaCCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                 <  1:125646/101‑1 (MQ=255)
                                                                      aCCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCgg                                >  1:2289756/1‑101 (MQ=255)
                                                                      aCCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCgg                                >  2:6617629/1‑101 (MQ=255)
                                                                        cgccgcGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGGTAAACCCCGATTTTCGGcc                              >  2:2012535/1‑101 (MQ=255)
                                                                        cgccgcGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGcc                              >  1:4522192/1‑101 (MQ=255)
                                                                            gcgcAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                          >  2:598955/1‑101 (MQ=255)
                                                                               cAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                       >  1:322900/1‑101 (MQ=255)
                                                                               cAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                       <  1:1036538/101‑1 (MQ=255)
                                                                                aaaaaGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCTGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAggg                      <  1:5366671/101‑1 (MQ=255)
                                                                                aaaaaGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAggg                      >  2:1713032/1‑101 (MQ=255)
                                                                                aaaaaGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAggg                      >  2:192181/1‑101 (MQ=255)
                                                                                aaaaaGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGCGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGGTTTTCGTCCCCTGAggg                      >  2:2213703/1‑101 (MQ=255)
                                                                                 aaaaGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgggg                     <  1:4276218/101‑1 (MQ=255)
                                                                                 aaaaGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgggg                     <  2:6179457/101‑1 (MQ=255)
                                                                                   aaGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGaa                   <  2:2629867/101‑1 (MQ=255)
                                                                                      cccGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGa                <  1:1144970/101‑1 (MQ=255)
                                                                                         gCCA.AGAGGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTc             <  1:3928875/101‑1 (MQ=255)
                                                                                         gCCA.AGAAGCGGCGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTc             >  1:6405624/1‑101 (MQ=255)
                                                                                          ccA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAACCCCCGATTTTCGGCCCCTGAGGGGAAAGATTCt            <  1:5006301/101‑1 (MQ=255)
                                                                                          ccA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCt            >  1:1902838/1‑101 (MQ=255)
                                                                                           cA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCtt           >  1:6748112/1‑101 (MQ=255)
                                                                                            a.agaagCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTg          <  1:302222/101‑1 (MQ=255)
                                                                                              agaagGGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTgg         >  2:3514939/1‑101 (MQ=255)
                                                                                              agaagCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTgg         >  2:1317205/1‑101 (MQ=255)
                                                                                              agaagCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTgg         <  1:2100059/101‑1 (MQ=255)
                                                                                                  gCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcaca     <  1:6991080/101‑1 (MQ=255)
                                                                                                  gCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcaca     <  1:1819421/101‑1 (MQ=255)
                                                                                                   cGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACaa    <  1:2025855/101‑1 (MQ=255)
                                                                                                    ggAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAt   >  1:3794490/1‑101 (MQ=255)
                                                                                                     gAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAtt  <  2:1034840/101‑1 (MQ=255)
                                                                                                     gAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAtt  >  1:5823578/1‑101 (MQ=255)
                                                                                                                                                                                                          
AAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCA.AGAAGCGGAGCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATT  >  NC_014034/1397931‑1398131

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG