Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,5260T→C100.0% 2.5 6intergenic (‑305/‑653)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (0/0):  total (3/3)

CTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCCGCGGCGTTTGACCTGGTCGCGG..GCAGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1434474‑1434579
                                                    |                                                       
ctcgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGAt         >  1:3976481/8‑101 (MQ=0)
  cgatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  1:2537149/6‑101 (MQ=2)
    gtcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:6803068/98‑1 (MQ=2)
    atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  1:3864319/98‑1 (MQ=2)
      cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac   >  2:6380551/2‑101 (MQ=2)
       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTATGAGGGCTGGCGGCCCGGTCGTGGATCCCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca  <  1:3463995/101‑2 (MQ=2)
                                                    |                                                       
CTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCCGCGGCGTTTGACCTGGTCGCGG..GCAGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1434474‑1434579

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG