Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,504,1221.→G 0.8% 0.7 ‑2.1 ‑0.0372intergenic (‑15/+68)recO/RCAP_rcc03309DNA repair protein RecO/hypothetical protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (180/189):  total (183/189)
Fisher's exact test strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.22e-01

Only 100 of 418 total aligned reads displayed.

TGCGTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGT  >  NC_014034/3504022‑3504222
                                                                                                                                                                                                          
 gCGTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCCTGGTGCCCGTGACGAAg                                                                                                      >  2:6107013/1‑101 (MQ=255)
 gCGTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAg                                                                                                      >  1:4753954/1‑101 (MQ=255)
  cGTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAgg                                                                                                     <  1:5461896/101‑1 (MQ=255)
   gTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGc                                                                                                    >  2:4411298/1‑101 (MQ=255)
   gTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGACGAAAAGAAGGGGCCGCTGTCTTGCCAGCCCATGGTGCCCCCGACCAAGGc                                                                                                    >  1:776463/1‑101 (MQ=255)
    tGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGcc                                                                                                   <  1:4668167/101‑1 (MQ=255)
    tGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGcc                                                                                                   <  1:50311/101‑1 (MQ=255)
     ggCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.g                                                                                                 <  1:6119100/101‑1 (MQ=255)
          tGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.gggggg                                                                                            >  2:5217546/1‑100 (MQ=255)
          tGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGGGCCCGTGACGAAGGGC.gggggg                                                                                            >  2:6047928/1‑100 (MQ=255)
          tGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGGCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGGC.gggggg                                                                                            >  2:3450930/1‑100 (MQ=255)
            aaCACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.gggggggt                                                                                          >  2:2569507/1‑98 (MQ=255)
            aaCACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGGGCCCGTGACGAAGGCC.GGGGGgtt                                                                                          >  1:4538076/1‑101 (MQ=255)
             acacCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGtttt                                                                                         <  2:4862413/101‑1 (MQ=255)
             acacCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGtttt                                                                                         <  1:5122314/101‑1 (MQ=255)
             acacCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.CGGGGgttt                                                                                         >  2:2087588/1‑101 (MQ=255)
              cacCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGGGCCCGTGACGAAGGCC.CGGGGGTTTg                                                                                        >  2:3197176/1‑101 (MQ=255)
                 cTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCAc                                                                                     <  1:2212213/101‑1 (MQ=255)
                  tCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCAcc                                                                                    <  2:2772709/101‑1 (MQ=255)
                    gACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCAcccc                                                                                  <  1:4143557/101‑1 (MQ=255)
                    gACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGGAcccc                                                                                  >  1:5553381/1‑101 (MQ=255)
                     aCAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCAccccc                                                                                 <  1:1358148/101‑1 (MQ=255)
                     aCAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGGAccccc                                                                                 >  2:3961494/1‑101 (MQ=255)
                       aaTCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCgg                                                                               <  2:108974/101‑1 (MQ=255)
                          cGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGGC.GGGGGGTTTGCACCCCCGGGcc                                                                            >  1:1668279/1‑101 (MQ=255)
                           gCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGgcc                                                                           >  1:5382620/1‑101 (MQ=255)
                           gCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGccc                                                                           >  2:6813555/1‑101 (MQ=255)
                            ccGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCCCCCCCGGGcccc                                                                          >  2:3894116/1‑101 (MQ=255)
                              gAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGGGCCCGTGACGAAGGCC.GGGGGCTGTGGACCCCCGGGCCCTCg                                                                        >  2:7075786/1‑101 (MQ=255)
                              gAGCTTTCGCCGTGCAGGCGGGGCGAAAGCATGGGGCCGCTGGCTTGCCAGTCCATGGTGCCCGTGGCCAAGGGC.TGGGGCGTCGCACACCTAGACCCCCg                                                                        >  2:150371/1‑101 (MQ=255)
                               aGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGc                                                                       <  1:5987377/101‑1 (MQ=255)
                               aGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGccccccc                                                                       >  1:6421263/1‑99 (MQ=255)
                                 cTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAg                                                                     <  2:2703792/101‑1 (MQ=255)
                                    tCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGat                                                                  <  1:2953736/101‑1 (MQ=255)
                                     cGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGata                                                                 <  1:3747926/101‑1 (MQ=255)
                                        cGTGCAGGCGGGTCGAAAGGCAGGGGCCGCTGTCTTGCCAGGCCATGGTGCCCGGGACGAAGGCG.GGGGGGTTTTCTCCCCCCGGCCCCCGCGggcttgtt                                                              >  2:1871523/1‑95 (MQ=255)
                                        cGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCCGGATAttt                                                              >  2:240562/1‑101 (MQ=255)
                                         gTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCCGGGCCCCGCAGGGTATTTg                                                             >  2:4992110/1‑101 (MQ=255)
                                             aGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGc                                                         <  1:2087668/101‑1 (MQ=255)
                                             aGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGc                                                         <  1:1790342/101‑1 (MQ=255)
                                             aGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGc                                                         >  2:5068962/1‑101 (MQ=255)
                                             aGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGc                                                         >  2:6567821/1‑101 (MQ=255)
                                              ggCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCa                                                        <  1:3894279/101‑1 (MQ=255)
                                              ggCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCa                                                        <  2:5408403/101‑1 (MQ=255)
                                               gCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCaa                                                       <  2:776439/101‑1 (MQ=255)
                                                    tCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATaa                                                  <  1:4280296/101‑1 (MQ=255)
                                                     cGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATaaa                                                 <  1:669977/101‑1 (MQ=255)
                                                     cGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATaaa                                                 >  1:6882753/1‑101 (MQ=255)
                                                     cGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATaaa                                                 >  1:1637809/1‑101 (MQ=255)
                                                      gAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCCGGGGGGTTTTTCACCCCCGGCGGCCCCCGGGTATTTTGGGGTAAGATaaa                                                 >  2:1390651/1‑101 (MQ=255)
                                                      gAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCCGGCCCCCCCCGGATATTTGAGGCGAGATaaaa                                                >  1:2680065/1‑101 (MQ=255)
                                                         aGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGca                                             >  1:3872015/1‑100 (MQ=255)
                                                          ggATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGGGa                                            >  1:3531474/1‑101 (MQ=255)
                                                          ggATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGa                                            >  2:1186519/1‑101 (MQ=255)
                                                           gATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGaa                                           <  2:2979705/101‑1 (MQ=255)
                                                           gATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGaa                                           <  2:6886167/101‑1 (MQ=255)
                                                           gATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTTCACCCCCCGGCCCCCGCCGGGTATTTTAGGGAAGATAAAAGAGaa                                           >  1:2341245/1‑101 (MQ=255)
                                                            aTGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGaaa                                          >  2:3644344/1‑101 (MQ=255)
                                                            aTGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGaaa                                          >  2:3084429/1‑101 (MQ=255)
                                                             tGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAg                                         <  2:4052068/101‑1 (MQ=255)
                                                             tGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAg                                         <  2:213521/101‑1 (MQ=255)
                                                              ggTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGc                                        <  1:4992362/101‑1 (MQ=255)
                                                                tGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCg                                      >  2:5983231/1‑101 (MQ=255)
                                                                  ccGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCggg                                    <  1:6981274/101‑1 (MQ=255)
                                                                      tGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCa                                <  1:1745813/101‑1 (MQ=255)
                                                                      tGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGGCa                                >  2:1736000/1‑101 (MQ=255)
                                                                       gTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAg                               >  1:4005834/1‑101 (MQ=255)
                                                                        tCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGt                              <  1:4866727/101‑1 (MQ=255)
                                                                         cTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTc                             <  2:22342/101‑1 (MQ=255)
                                                                         cTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTc                             <  2:4149179/101‑1 (MQ=255)
                                                                          ttGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCt                            <  2:3786436/101‑1 (MQ=255)
                                                                          ttGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCt                            <  2:5559992/101‑1 (MQ=255)
                                                                          ttGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCt                            >  1:3059029/1‑101 (MQ=255)
                                                                          ttGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCt                            <  1:2907184/101‑1 (MQ=255)
                                                                           tGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGAAATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCtt                           >  2:6732622/1‑101 (MQ=255)
                                                                           tGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGGTATTTTGGGGCAGATAAAAGCGAAAGCCGGGGGCAGGCtt                           >  1:3365027/1‑101 (MQ=255)
                                                                             ccAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTc                         <  2:6386235/101‑1 (MQ=255)
                                                                              cAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCa                        <  1:1871595/101‑1 (MQ=255)
                                                                              cAGGCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCa                        <  2:6205832/101‑1 (MQ=255)
                                                                               aGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAg                       >  2:2544012/1‑101 (MQ=255)
                                                                                gTCCATGGTGCCCGTGACGAAGGCC.GGGGGGTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGCCAGTCTTTCAGt                      >  1:1031089/1‑101 (MQ=255)
                                                                                 tCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTc                     >  2:5552333/1‑101 (MQ=255)
                                                                                   cATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGGACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCg                   >  1:6196046/1‑101 (MQ=255)
                                                                                    aTGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGCCTTTCAGTCCGt                  >  2:4496792/1‑101 (MQ=255)
                                                                                     tGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTc                 <  1:6603658/101‑1 (MQ=255)
                                                                                     tGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTc                 >  2:736298/1‑101 (MQ=255)
                                                                                        tGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTc              <  1:1406330/101‑1 (MQ=255)
                                                                                         gCCCGTGACGAAGGCC.GGGGGGTTTTCACCCCCCGGCCCCCCCAGGATATTTGAGGCAAGATAAAAGCGAAAACCGGGGTCAGTCTTTCAGTCCGTCTTCa             >  1:2464155/1‑101 (MQ=38)
                                                                                          cccGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGGCAGTCTTTCAGGCCGTCTTCAt            >  1:978878/1‑101 (MQ=255)
                                                                                           ccGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATc           >  2:5816111/1‑101 (MQ=255)
                                                                                            cGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATcc          <  2:3307968/101‑1 (MQ=255)
                                                                                              tGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAg        >  1:6844154/1‑101 (MQ=255)
                                                                                                aCGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGaa      <  2:1637748/101‑1 (MQ=255)
                                                                                                aCGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGaa      <  1:2749623/101‑1 (MQ=255)
                                                                                                  gAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGc    >  1:5849656/1‑101 (MQ=255)
                                                                                                  gAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGc    <  1:5217811/101‑1 (MQ=255)
                                                                                                  gAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGGCAGTCTTTCAGTCCGTCTTCATCCAGAAGc    >  1:2571525/1‑101 (MQ=255)
                                                                                                   aaGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCg   >  1:3246691/1‑101 (MQ=255)
                                                                                                    aGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCgc  >  1:5742326/1‑100 (MQ=255)
                                                                                                    aGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGt  <  1:1186567/101‑1 (MQ=255)
                                                                                                                                                                                                          
TGCGTGGCCGTGAACACCTCGACAATCGCCGAGCTTTCGCCGTGCAGGCGGGTCGAAAGGATGGTGCCGCTGTCTTGCCAGTCCATGGTGCCCGTGACGAAGGCC.GGGGGTTTTGCACCCCCGGGCCCCCGCAGGATATTTGAGGCAAGATAAAAGCGAAAGCCGGGGTCAGTCTTTCAGTCCGTCTTCATCCAGAAGCGT  >  NC_014034/3504022‑3504222

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG