Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,535 T→C 18.4% intergenic (+347/‑584) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5350T→C18.4% 168.8 ‑1.5 ‑1.7509intergenic (+347/‑584)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/39):  ref base (177/170):  total (253/256)
Fisher's exact test strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.84e-02

Only 100 of 576 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTC  >  NC_014034/1395434‑1395635
                                                                                                     |                                                                                                       
 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                                                                                         >  1:5659688/1‑101 (MQ=255)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                                                       <  2:6248931/101‑1 (MQ=255)
    cgccccgcGCGGGGGCGTGGATCGCACCATCGCCGCTTTGGCCGTCGACAGGCTCGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                                                                                      <  2:65015/99‑1 (MQ=37)
    cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                                                                                     >  1:6738877/1‑97 (MQ=2)
     tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                                                                                    <  2:3034344/101‑6 (MQ=2)
      ccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                                                                                   >  2:3693916/1‑95 (MQ=0)
       cccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatg                                                                                                  <  2:335451/101‑8 (MQ=2)
        ccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                                                                                 <  2:3421401/101‑9 (MQ=2)
          ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                                                                                >  1:1891619/1‑101 (MQ=255)
          ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                                                                                >  1:219773/1‑101 (MQ=255)
              gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                                                                                            <  1:1727795/101‑1 (MQ=255)
               ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                                                                                           <  2:1380059/101‑1 (MQ=255)
                gggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCg                                                                                          >  1:3928541/1‑101 (MQ=255)
                    gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tccc                                                                                     >  2:1739307/1‑100 (MQ=255)
                     tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                    >  2:4308110/1‑101 (MQ=255)
                      ttcaagAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAga                                                                                   >  2:2895894/6‑100 (MQ=255)
                      ggATCGAAACATCGCCGCTTTGGCGGTCAACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.GCCCGc                                                                                   >  1:3448379/1‑101 (MQ=255)
                          cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACATCTCGGTGCCGTCGCTCG.TCCAGCCTG.c                                                                              >  2:7067430/1‑101 (MQ=255)
                           gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                             >  1:4575201/1‑101 (MQ=255)
                           gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                             <  1:5350350/101‑1 (MQ=255)
                             aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                          >  1:6098403/1‑101 (MQ=255)
                              aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.Gc                                                                         <  2:4771977/101‑1 (MQ=255)
                               cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTGGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCt                                                                        <  2:2443034/101‑1 (MQ=255)
                                aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtc                                                                       <  2:2364148/101‑1 (MQ=255)
                                aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtc                                                                       >  1:2880381/1‑101 (MQ=255)
                                 tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcg                                                                      <  1:644849/101‑1 (MQ=255)
                                   tctgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcgtc                                                                    <  1:6156252/98‑1 (MQ=255)
                                      gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCt                                                                 <  2:6306192/101‑1 (MQ=255)
                                       cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTc                                                                >  1:6594888/1‑101 (MQ=255)
                                       cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTc                                                                >  1:2404489/1‑101 (MQ=255)
                                            gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcccccc                                                           <  2:5440726/101‑1 (MQ=255)
                                              ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgc                                                         <  1:1780708/101‑1 (MQ=255)
                                               gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TGCCGCCGG.CCT.GCTCGTCGCTCCCCCcccg                                                        >  2:6163043/1‑101 (MQ=255)
                                                     aGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCg                                                  >  2:4053940/1‑101 (MQ=255)
                                                      ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                 <  1:4187910/101‑1 (MQ=255)
                                                       gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                >  2:217766/1‑101 (MQ=255)
                                                       gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCCCGGGGGCGTg                                                >  1:3290636/1‑101 (MQ=255)
                                                       gCTGGCAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGCTGCCGTCGCTCG.TCCAGCCGG.CCC.GCGCGTCGCCACCCCCCCGGCGGCCTg                                                >  2:6723102/1‑101 (MQ=25)
                                                        aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:1053672/91‑1 (MQ=2)
                                                          ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:4069645/101‑1 (MQ=255)
                                                          cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:1042213/93‑1 (MQ=2)
                                                           gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:2687919/1‑101 (MQ=255)
                                                           ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:1563365/94‑1 (MQ=2)
                                                           ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:2614646/94‑1 (MQ=2)
                                                            tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:3627124/95‑1 (MQ=2)
                                                            taccgcgtCGCTCCCCCCGCTGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:5593017/95‑1 (MQ=2)
                                                            tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:3809706/101‑1 (MQ=255)
                                                             cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  2:576294/6‑101 (MQ=2)
                                                             accGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  2:3228460/99‑1 (MQ=255)
                                                              gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:6974482/97‑1 (MQ=2)
                                                              gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:5190702/97‑1 (MQ=2)
                                                               cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:1817711/101‑1 (MQ=255)
                                                                tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                       <  2:2756846/99‑1 (MQ=2)
                                                                gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                       <  1:4346602/101‑1 (MQ=255)
                                                                ccgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                       <  1:6267312/99‑1 (MQ=2)
                                                                acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                       >  2:5278950/3‑101 (MQ=2)
                                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAaccc                                     >  2:6615877/1‑100 (MQ=255)
                                                                  gtCGCTCCCCCCGCGGGGGCGTGGAGCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                     <  2:886510/101‑1 (MQ=255)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                    >  1:5742484/1‑99 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                   >  1:2771360/1‑101 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                   <  2:6071020/101‑4 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                   >  1:3514241/1‑97 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                   <  1:4870860/101‑5 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                  <  1:3006157/101‑1 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATccgaactgagt                                  >  2:5012884/1‑91 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                  >  1:1432997/1‑96 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                  >  2:204731/1‑96 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                  >  1:1129728/1‑97 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                  >  2:4196589/1‑96 (MQ=2)
                                                                     ccTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                  <  2:1544939/100‑5 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                 <  2:749328/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                 >  2:5370035/1‑96 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TTCAGGCGG.CCC.GCTCGTCGATCCCCCCGCGGGGGCGGGGATCGAAACCACCTTg                                >  2:4585460/1‑101 (MQ=37)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                >  1:233886/1‑94 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcgg                                >  2:1659927/1‑95 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                >  2:2611488/1‑94 (MQ=2)
                                                                        ccccccGCGGGGTCGTCGGCCAAAACATCTCGGTGCCGTCGCTCG.TCCAGTCGG.CCC.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                               <  1:7067804/101‑1 (MQ=25)
                                                                        ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                               >  2:5162555/1‑93 (MQ=2)
                                                                        ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                               <  1:3075950/101‑9 (MQ=2)
                                                                         cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                              <  1:204734/101‑9 (MQ=0)
                                                                          ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                             >  1:6331641/1‑101 (MQ=255)
                                                                             cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                          <  1:1224457/101‑1 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                         <  2:1900335/101‑1 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                         <  1:1932213/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                        >  1:5999843/1‑101 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                        >  1:2548852/1‑101 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                        <  2:5927532/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                       <  1:721727/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                       <  1:5541937/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                       <  2:483657/101‑1 (MQ=255)
                                                                                 ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                      <  2:19898/101‑1 (MQ=255)
                                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                 >  2:1125884/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                 <  1:1606054/101‑1 (MQ=255)
                                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                 >  2:6921959/1‑101 (MQ=255)
                                                                                         gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg              >  1:854342/1‑101 (MQ=255)
                                                                                         gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg              >  1:465423/1‑101 (MQ=255)
                                                                                           tCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg            >  1:4445581/1‑101 (MQ=255)
                                                                                             gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc          >  1:916201/1‑101 (MQ=255)
                                                                                               aaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa        <  2:5636286/101‑1 (MQ=255)
                                                                                                 cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg      >  2:4464570/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTC  >  NC_014034/1395434‑1395635

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG