Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,536 C→A 7.8% intergenic (+348/‑583) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5360C→A 7.8% 89.9 ‑0.1 ‑0.0511intergenic (+348/‑583)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/21):  ref base (234/237):  total (253/258)
Fisher's exact test strand distribution p-value = 8.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 546 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCG  >  NC_014034/1395436‑1395636
                                                                                                    |                                                                                                        
cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                                                                                          <  1:6217824/101‑1 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                                                         >  1:767812/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                                                         <  2:6248931/101‑1 (MQ=255)
  cgccccgcGCGGGGGCGTGGATCGCACCATCGCCGCTTTGGCCGTCGACAGGCTCGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                                                                                        <  2:65015/99‑1 (MQ=37)
   tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                                                                                       <  1:30720/101‑1 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                                                                                      >  1:4401164/1‑101 (MQ=255)
      ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGcc                                                                                                    <  2:2004257/101‑1 (MQ=255)
      ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGcc                                                                                                    <  2:4378739/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                                                                                  >  1:1891619/1‑101 (MQ=255)
            gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                                                                                              <  1:1727795/101‑1 (MQ=255)
             ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                                                                                             >  2:1677370/1‑101 (MQ=255)
                 cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                        <  1:7095824/101‑1 (MQ=255)
                   tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                      >  1:4167159/1‑101 (MQ=255)
                    ggATCGAAACATCGCCGCTTTGGCGGTCAACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.GCCCGc                                                                                     >  1:3448379/1‑101 (MQ=255)
                     gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGcc                                                                                    >  2:758049/1‑101 (MQ=255)
                         gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                               <  1:5350350/101‑1 (MQ=255)
                          aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGATCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                              >  2:3388313/1‑101 (MQ=255)
                           aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                            <  1:2240420/101‑1 (MQ=255)
                              agcgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tc                                                                        <  2:4670891/99‑1 (MQ=255)
                              aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tc                                                                        >  1:2880381/1‑101 (MQ=255)
                                cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tcgt                                                                      <  1:1409203/101‑1 (MQ=255)
                                 gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tcgtc                                                                     <  1:3918751/101‑1 (MQ=255)
                                 gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGCGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tcgtc                                                                     >  1:483669/1‑101 (MQ=255)
                                     cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTc                                                                 >  1:2404489/1‑101 (MQ=255)
                                     cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTc                                                                 <  2:2070953/101‑1 (MQ=255)
                                          gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTcccccc                                                            <  2:1012228/101‑1 (MQ=255)
                                            ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCcgc                                                          <  1:1780708/101‑1 (MQ=255)
                                             gTCGACGGGCTGGTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCcgcg                                                         <  2:1470264/101‑1 (MQ=255)
                                             gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TGCCGCCGG.CCT.GC.TCGTCGCTCCCCCcccg                                                         >  2:6163043/1‑101 (MQ=255)
                                              tCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCgg                                                        >  1:4334673/1‑101 (MQ=255)
                                               cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCggg                                                       >  1:1221702/1‑101 (MQ=255)
                                               cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCggg                                                       >  2:1822824/1‑101 (MQ=255)
                                                gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCgggg                                                      >  2:6327832/1‑101 (MQ=255)
                                                  cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGc                                                    <  2:1753228/101‑1 (MQ=255)
                                                    ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                  >  1:4558014/1‑101 (MQ=255)
                                                     gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCCCGGGGGCGTg                                                 >  1:3290636/1‑101 (MQ=255)
                                                     gCTGGCAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGCTGCCGTCGCTCG.TCCAGCCGG.CCC.GC.GCGTCGCCACCCCCCCGGCGGCCTg                                                 >  2:6723102/1‑101 (MQ=25)
                                                      gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                <  1:901757/91‑1 (MQ=0)
                                                      cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:5493055/1‑101 (MQ=255)
                                                      aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTgg                                                <  2:6628474/91‑1 (MQ=2)
                                                      aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTgg                                                <  2:5672885/91‑1 (MQ=2)
                                                      aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTgg                                                >  1:6493731/11‑101 (MQ=2)
                                                       tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                               <  1:2766275/101‑1 (MQ=255)
                                                       tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCG.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                               >  2:54058/1‑101 (MQ=255)
                                                        ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  2:192284/93‑1 (MQ=2)
                                                        ggccggtggtCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  2:2786868/93‑1 (MQ=2)
                                                        cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  1:1042213/93‑1 (MQ=2)
                                                         ctcgcgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:496470/94‑1 (MQ=2)
                                                         ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:1563365/94‑1 (MQ=2)
                                                         ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:2614646/94‑1 (MQ=2)
                                                         ctcgcacgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:7026419/94‑1 (MQ=2)
                                                          tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            <  1:3627124/95‑1 (MQ=2)
                                                          taccgcgtCGCTCCCCCCGCTGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:5593017/95‑1 (MQ=2)
                                                          tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            <  1:3809706/101‑1 (MQ=255)
                                                          ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            <  2:5811267/95‑1 (MQ=2)
                                                           aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           >  1:6665508/1‑101 (MQ=255)
                                                            ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          >  2:5323126/5‑101 (MQ=2)
                                                            gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          >  2:2930367/5‑101 (MQ=2)
                                                            gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:5190702/97‑1 (MQ=2)
                                                             gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         >  1:5866552/4‑101 (MQ=2)
                                                             cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  1:6312412/98‑1 (MQ=2)
                                                             cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  2:7080361/101‑1 (MQ=255)
                                                              gtgtCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                        <  2:2703251/101‑1 (MQ=255)
                                                               cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGT.G.TCAGGTTTT.CCA.GCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                       >  1:1622006/2‑101 (MQ=2)
                                                               cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                       >  2:1613089/2‑100 (MQ=2)
                                                                gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                      >  2:3680484/1‑101 (MQ=255)
                                                                gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                      <  2:6965040/101‑3 (MQ=2)
                                                                gtCGCTCCCCCCGCGGGGGCGTGGAGCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                      <  2:886510/101‑1 (MQ=255)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                     <  2:2520841/101‑3 (MQ=2)
                                                                 tCGCTCCCCCCGCGGCGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                     <  2:5201044/101‑3 (MQ=2)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                    <  2:6071020/101‑4 (MQ=2)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                    <  2:128664/101‑5 (MQ=2)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                    >  1:3514241/1‑97 (MQ=2)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                   <  1:3006157/101‑1 (MQ=255)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaacctgcgg                                   >  2:5974205/1‑94 (MQ=2)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgg                                   >  2:4299365/1‑97 (MQ=2)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                   >  1:1129728/1‑97 (MQ=2)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                 <  1:406586/101‑8 (MQ=2)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                 >  1:233886/1‑94 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                >  2:1102029/1‑101 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                >  1:6055715/1‑93 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                >  2:4559050/1‑94 (MQ=2)
                                                                      ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                >  2:3115889/1‑94 (MQ=2)
                                                                        ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                              <  1:5682331/101‑1 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                              <  1:1944764/101‑11 (MQ=2)
                                                                         cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                             <  2:5141672/101‑1 (MQ=255)
                                                                          ccGCGGGGGCGCGTCGCGAACCATCTCGCTGCCGTCGCTCG.CCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                            <  2:3305169/101‑1 (MQ=25)
                                                                             cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                         >  1:2548852/1‑101 (MQ=255)
                                                                              gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                        >  2:2405878/1‑101 (MQ=255)
                                                                              gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                        <  1:5541937/101‑1 (MQ=255)
                                                                               ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                       >  2:3924870/1‑101 (MQ=255)
                                                                                  gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                    <  1:2073807/101‑1 (MQ=255)
                                                                                     tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                 >  2:4173523/1‑101 (MQ=255)
                                                                                     tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                 >  1:6846739/1‑101 (MQ=255)
                                                                                       gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg               >  1:465423/1‑101 (MQ=255)
                                                                                           gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc           >  2:2237029/1‑101 (MQ=255)
                                                                                             aaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa         >  1:6124817/1‑101 (MQ=255)
                                                                                             aaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa         <  1:6400747/101‑1 (MQ=255)
                                                                                                 tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg     <  2:2629236/101‑1 (MQ=255)
                                                                                                  ctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt    <  2:4817058/101‑1 (MQ=255)
                                                                                                    |                                                                                                        
CGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCG  >  NC_014034/1395436‑1395636

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG