Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,543 G→A 1.8% intergenic (+355/‑576) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5430G→A 1.8% 11.8 0.0 ‑0.3509intergenic (+355/‑576)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/5):  ref base (244/256):  total (248/261)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.50e-01

Only 100 of 534 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCC  >  NC_014034/1395443‑1395643
                                                                                                    |                                                                                                       
    cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                                                                                     <  1:644982/101‑1 (MQ=255)
      ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                                                                                                   <  2:1380059/101‑1 (MQ=255)
        ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.t                                                                                                <  2:447364/101‑1 (MQ=255)
          cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                              <  1:7136954/101‑1 (MQ=255)
           gTGGATCGAAACCTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                             >  1:6306516/1‑101 (MQ=255)
           gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                             <  1:2818699/101‑1 (MQ=255)
           gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                             <  2:5348981/101‑1 (MQ=255)
            tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                            >  1:4167159/1‑101 (MQ=255)
             ttcaagAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAga                                                                                           >  2:2895894/6‑100 (MQ=255)
             ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGCCGCTCG.TCCAGc                                                                                           >  2:3910154/1‑101 (MQ=255)
             ggATCGAAACATCGCCGCTTTGGCGGTCAACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.GCCCGc                                                                                           >  1:3448379/1‑101 (MQ=255)
              gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGcc                                                                                          >  2:758049/1‑101 (MQ=255)
                 cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACATCTCGGTGCCGTCGCTCG.TCCAGCCTG.c                                                                                      >  2:7067430/1‑101 (MQ=255)
                 cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c                                                                                      >  2:5541647/1‑101 (MQ=255)
                  gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                     <  1:5350350/101‑1 (MQ=255)
                  gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                     >  1:4575201/1‑101 (MQ=255)
                  gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                     >  2:4270055/1‑101 (MQ=255)
                    aaCATCGCCGCTTTGGCGGTCGACTGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTg                                                                                   <  2:1813697/101‑1 (MQ=255)
                    aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTg                                                                                   >  2:5682041/1‑101 (MQ=255)
                    aaCAGCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTg                                                                                   <  1:559098/101‑1 (MQ=255)
                      cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTGGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.t                                                                                <  2:2443034/101‑1 (MQ=255)
                       aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.tc                                                                               <  2:1891547/101‑1 (MQ=255)
                          gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.tcgtc                                                                            <  1:3918751/101‑1 (MQ=255)
                          gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGCGCCGTCGCTCG.TCCAGCCGG.CCTGC.tcgtc                                                                            >  1:483669/1‑101 (MQ=255)
                             gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCt                                                                         >  2:4336335/1‑101 (MQ=255)
                             gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCt                                                                         >  1:2473712/1‑101 (MQ=255)
                                ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTccc                                                                      >  2:3450246/1‑101 (MQ=255)
                                    cGGGCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCcg                                                                  <  1:6767823/101‑1 (MQ=255)
                                       tCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCgg                                                               >  1:4334673/1‑101 (MQ=255)
                                            aGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCg                                                          >  1:5749150/1‑101 (MQ=255)
                                              gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTg                                                        >  2:217766/1‑101 (MQ=255)
                                              gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTg                                                        >  1:6382314/1‑101 (MQ=255)
                                              gCTGGCAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGCTGCCGTCGCTCG.TCCAGCCGG.CCCGC.GCGTCGCCACCCCCCCGGCGGCCTg                                                        >  2:6723102/1‑101 (MQ=25)
                                               gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                       <  2:1054673/91‑1 (MQ=0)
                                               agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG..GCGTCGCTCCCCCCGCGGGGGCGTgg                                                       >  2:5993045/11‑101 (MQ=0)
                                               agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG..GCGTCGCTCCCCCCGCGGGGGCGTgg                                                       <  2:2388445/91‑1 (MQ=0)
                                                tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTTCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                      <  2:4999435/101‑1 (MQ=255)
                                                ccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGa                                                      >  2:47150/10‑101 (MQ=2)
                                                 ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGG.ATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  1:2375785/93‑1 (MQ=2)
                                                 ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     >  1:4400659/1‑101 (MQ=255)
                                                 cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  1:1042213/93‑1 (MQ=2)
                                                  gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                    >  2:2687919/1‑101 (MQ=255)
                                                   tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:3627124/95‑1 (MQ=2)
                                                   tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:3203866/101‑1 (MQ=255)
                                                    accGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  <  2:3228460/99‑1 (MQ=255)
                                                    aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  >  2:6592185/1‑101 (MQ=255)
                                                     gtccgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 >  2:1726633/5‑101 (MQ=2)
                                                     ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 >  1:1566753/5‑101 (MQ=2)
                                                     ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 >  1:2595/5‑101 (MQ=2)
                                                     gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                 <  1:5190702/97‑1 (MQ=2)
                                                      gcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                <  1:8616/98‑1 (MQ=2)
                                                      cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                >  2:5234102/4‑101 (MQ=2)
                                                      cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                <  2:1221654/101‑1 (MQ=255)
                                                       tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                               <  1:2939934/99‑1 (MQ=2)
                                                       tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                               <  2:2756846/99‑1 (MQ=2)
                                                       cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                               <  1:1847456/99‑1 (MQ=2)
                                                       ccgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                               <  2:3045167/99‑1 (MQ=2)
                                                        cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGT.G.TCAGGTTTT.CCAGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                              >  1:1622006/2‑101 (MQ=2)
                                                         gtCGCTCCCCCCGCGGGGGCGTGGAGCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                             <  2:886510/101‑1 (MQ=255)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                           >  1:1778433/1‑101 (MQ=255)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                           >  1:2671184/1‑101 (MQ=255)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcg                                           >  2:5437696/1‑97 (MQ=2)
                                                           cGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcg                                           <  1:3503571/101‑5 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                          >  2:6096200/1‑96 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                          >  2:204731/1‑96 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                          >  1:1129728/1‑97 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                          >  1:6976770/1‑96 (MQ=2)
                                                            ccTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                          <  2:1544939/100‑5 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                         >  2:1982530/1‑95 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TTCAGGCGG.CCCGC.TCGTCGATCCCCCCGCGGGGGCGGGGATCGAAACCACCTTg                                        >  2:4585460/1‑101 (MQ=37)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                        <  1:406586/101‑8 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                        >  2:2611488/1‑94 (MQ=2)
                                                               ccccccGCGGGGTCGTCGGCCAAAACATCTCGGTGCCGTCGCTCG.TCCAGTCGG.CCCGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                       <  1:7067804/101‑1 (MQ=25)
                                                               ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                       >  1:6055715/1‑93 (MQ=2)
                                                               ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCCGC.CCGTCGCGCCCCCCCCGGGGGCGGGGATCGAAactgggtgcg                                       >  1:2290816/1‑93 (MQ=2)
                                                               ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACAGG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                       >  2:1653188/1‑93 (MQ=2)
                                                                cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCCG..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                      <  1:204734/101‑9 (MQ=0)
                                                                  cccGCGGGGGCGTGGATCGAAACATGGCTGTGGCGGCAGT.G.TCAGGTTTT.CCAGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccgccacgtct                                    <  2:3386594/101‑11 (MQ=0)
                                                                  cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                                    <  2:5141672/101‑1 (MQ=255)
                                                                   ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                   >  2:6268553/1‑101 (MQ=255)
                                                                    cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                  <  1:1224457/101‑1 (MQ=255)
                                                                     gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                 <  1:1932213/101‑1 (MQ=255)
                                                                       gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                               >  1:6119420/1‑101 (MQ=255)
                                                                       gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                               <  2:483657/101‑1 (MQ=255)
                                                                          ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                            >  2:4078252/1‑101 (MQ=255)
                                                                             gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                         >  2:6921959/1‑101 (MQ=255)
                                                                             gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                         <  1:3025115/101‑1 (MQ=255)
                                                                             gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                         <  2:6179459/101‑1 (MQ=255)
                                                                               ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                       >  2:6359760/1‑101 (MQ=255)
                                                                                gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                      >  1:6663528/1‑101 (MQ=255)
                                                                                gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                      >  1:465423/1‑101 (MQ=255)
                                                                                   cGAAACATCGCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg                   <  1:3800053/101‑1 (MQ=255)
                                                                                    gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                  >  1:92789/1‑101 (MQ=255)
                                                                                       aCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa               <  1:5637064/101‑1 (MQ=255)
                                                                                          tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg            <  1:218681/101‑1 (MQ=255)
                                                                                             cGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg         <  2:5875549/101‑1 (MQ=255)
                                                                                             cGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg         >  1:142237/1‑101 (MQ=255)
                                                                                               gTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt       >  1:6188748/1‑101 (MQ=255)
                                                                                                 gCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc     <  1:54059/101‑1 (MQ=255)
                                                                                                  ccGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc    <  1:1739371/101‑1 (MQ=255)
                                                                                                    |                                                                                                       
CCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCTGC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCC  >  NC_014034/1395443‑1395643

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG