Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,547 C→A 5.9% intergenic (+359/‑572) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5470C→A 5.9% 63.1 0.0 ‑0.0509intergenic (+359/‑572)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/16):  ref base (231/246):  total (246/263)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 538 total aligned reads displayed.

CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCG  >  NC_014034/1395447‑1395647
                                                                                                    |                                                                                                        
cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                                                                                          <  1:644982/101‑1 (MQ=255)
  ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                                                                                                        <  2:1380059/101‑1 (MQ=255)
      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                                   <  1:7136954/101‑1 (MQ=255)
       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                  <  2:219770/101‑1 (MQ=255)
        tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                                 >  1:3452898/1‑101 (MQ=255)
         ttcaagAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAga                                                                                                >  2:2895894/6‑100 (MQ=255)
         ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGCCGCTCG.TCCAGc                                                                                                >  2:3910154/1‑101 (MQ=255)
              gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                          >  2:4270055/1‑101 (MQ=255)
               aaaCATCGCCACTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                         <  1:6294285/101‑1 (MQ=255)
                aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                       >  2:5682041/1‑101 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tc                                                                                   >  1:2880381/1‑101 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tc                                                                                   <  2:1891547/101‑1 (MQ=255)
                     cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCTGGGCCGTCGCTCG.CCCAACCGG.CCT.GG.tcgt                                                                                 >  1:1917222/1‑101 (MQ=37)
                      gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGCGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tcgtc                                                                                >  1:483669/1‑101 (MQ=255)
                         gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCt                                                                             >  2:4336335/1‑101 (MQ=255)
                          cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTc                                                                            <  2:2070953/101‑1 (MQ=255)
                          cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTc                                                                            >  1:6594888/1‑101 (MQ=255)
                          cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTc                                                                            >  1:2404489/1‑101 (MQ=255)
                           tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTcc                                                                           >  2:3252295/1‑101 (MQ=255)
                            ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTccc                                                                          >  2:3450246/1‑101 (MQ=255)
                              ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTccccc                                                                        >  2:2457311/1‑101 (MQ=255)
                                   tCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCgg                                                                   >  1:4334673/1‑101 (MQ=255)
                                        aGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCg                                                              >  1:5749150/1‑101 (MQ=255)
                                         ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                             >  2:6798173/1‑101 (MQ=255)
                                         ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                             >  1:4558014/1‑101 (MQ=255)
                                         ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                             <  1:4187910/101‑1 (MQ=255)
                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTg                                                            >  1:6382314/1‑101 (MQ=255)
                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCCCGGGGGCGTg                                                            >  1:3290636/1‑101 (MQ=255)
                                           gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:1054673/91‑1 (MQ=0)
                                           agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:2388445/91‑1 (MQ=0)
                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  1:4425985/101‑1 (MQ=255)
                                            ccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGa                                                          >  2:47150/10‑101 (MQ=2)
                                             ggccggtggtCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:2786868/93‑1 (MQ=2)
                                             ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:4069645/101‑1 (MQ=255)
                                             cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:1042213/93‑1 (MQ=2)
                                              gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        >  1:5587694/8‑101 (MQ=2)
                                              gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:4640774/94‑1 (MQ=2)
                                              ctcgcgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:496470/94‑1 (MQ=2)
                                               ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:6378271/95‑1 (MQ=2)
                                                aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:2629332/1‑101 (MQ=255)
                                                 gtccgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  2:1726633/5‑101 (MQ=2)
                                                 ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  2:5323126/5‑101 (MQ=2)
                                                 ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  1:534743/5‑101 (MQ=2)
                                                 ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  1:1566753/5‑101 (MQ=2)
                                                 gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  1:6974482/97‑1 (MQ=2)
                                                  gcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  1:8616/98‑1 (MQ=2)
                                                  cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  2:93175/98‑1 (MQ=2)
                                                  cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  2:3371170/98‑1 (MQ=2)
                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  2:7080361/101‑1 (MQ=255)
                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  2:1221654/101‑1 (MQ=255)
                                                   gtgtCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                   <  2:2703251/101‑1 (MQ=255)
                                                   acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                   <  1:6010375/99‑1 (MQ=2)
                                                    tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                  <  1:3529605/101‑1 (MQ=255)
                                                    cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGT.G.TCAGGTTTT.CCA.GCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                  >  1:1622006/2‑101 (MQ=2)
                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAaccc                                                 >  2:6615877/1‑100 (MQ=255)
                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                 <  2:6965040/101‑3 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                >  1:3992433/1‑101 (MQ=255)
                                                      tCGCTCCCCCCGCGGCGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                <  2:5201044/101‑3 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                               >  1:1778433/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                               >  1:2771360/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcg                                               >  2:5437696/1‑97 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                              >  1:1129728/1‑97 (MQ=2)
                                                        ccTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                              <  2:1544939/100‑5 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                             >  2:1982530/1‑95 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                             <  1:6741643/101‑7 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                            >  1:233886/1‑94 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                            >  2:2611488/1‑94 (MQ=2)
                                                           ccccccGCGGGGTCGTCGGCCAAAACATCTCGGTGCCGTCGCTCG.TCCAGTCGG.CCC.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                           <  1:7067804/101‑1 (MQ=25)
                                                           ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                           >  2:3115889/1‑94 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                           <  2:5991747/101‑9 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                           <  2:5519890/101‑9 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                           >  2:1653188/1‑93 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                           <  1:3075950/101‑9 (MQ=2)
                                                              cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                                        <  2:5141672/101‑1 (MQ=255)
                                                               ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                       >  2:6268553/1‑101 (MQ=255)
                                                                cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                      <  1:1224457/101‑1 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                    <  2:6098091/101‑1 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                    >  1:2548852/1‑101 (MQ=255)
                                                                   gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                   >  1:6119420/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                  >  2:6210752/1‑101 (MQ=255)
                                                                      ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                >  2:4078252/1‑101 (MQ=255)
                                                                       gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                               <  1:2073807/101‑1 (MQ=255)
                                                                        cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                              <  1:3882729/101‑1 (MQ=255)
                                                                        cGTGGATCGAAACATCTCGGTGCCGTCCCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                              <  1:2266223/101‑1 (MQ=255)
                                                                            gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                          >  2:1815589/1‑101 (MQ=255)
                                                                                gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                      >  1:92789/1‑101 (MQ=255)
                                                                                  accATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                    <  2:4574962/99‑1 (MQ=255)
                                                                                   aCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa                   <  1:5637064/101‑1 (MQ=255)
                                                                                    cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                  >  2:4464570/1‑101 (MQ=255)
                                                                                     aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                 <  1:51110/101‑1 (MQ=255)
                                                                                     aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                 <  2:767788/101‑1 (MQ=255)
                                                                                       ctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt               <  2:4817058/101‑1 (MQ=255)
                                                                                        tcGGTGCCGTCGCTCG.TCCAGCNGN.CNT.GN.NCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc              <  1:2965011/101‑1 (MQ=255)
                                                                                         cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg             >  1:142237/1‑101 (MQ=255)
                                                                                         cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg             <  2:5875549/101‑1 (MQ=255)
                                                                                           gTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt           >  1:6188748/1‑101 (MQ=255)
                                                                                             gCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc         <  1:54059/101‑1 (MQ=255)
                                                                                              ccGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc        <  1:1739371/101‑1 (MQ=255)
                                                                                                 tCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc     <  1:1935954/101‑1 (MQ=255)
                                                                                                   gCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc   >  1:3696156/1‑101 (MQ=255)
                                                                                                    |                                                                                                        
CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCG  >  NC_014034/1395447‑1395647

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG