Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,549 C→A 24.0% intergenic (+361/‑570) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5490C→A24.0% 0.0 ‑0.3 ‑1.3506intergenic (+361/‑570)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (57/64):  ref base (187/196):  total (245/261)
Fisher's exact test strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.80e-02

Only 100 of 533 total aligned reads displayed.

GGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGG  >  NC_014034/1395449‑1395649
                                                                                                    |                                                                                                        
  ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.t                                                                                                       <  2:447364/101‑1 (MQ=255)
    cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                                     <  1:4589218/101‑1 (MQ=255)
     gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tccc                                                                                                    >  2:1739307/1‑100 (MQ=255)
     gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                    <  1:2818699/101‑1 (MQ=255)
     gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                    <  2:6398578/101‑1 (MQ=255)
        gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGcc                                                                                                 <  1:3896971/101‑1 (MQ=255)
        gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGcc                                                                                                 <  2:2126430/101‑1 (MQ=255)
            gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.cc                                                                                            >  2:4270055/1‑101 (MQ=255)
             aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                           <  1:1576947/101‑1 (MQ=255)
             aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                           <  1:1241700/101‑1 (MQ=255)
              aaCAGCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                         <  1:559098/101‑1 (MQ=255)
                 aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tc                                                                                     >  2:1107579/1‑101 (MQ=255)
                       gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCt                                                                               <  2:6306192/101‑1 (MQ=255)
                       gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCt                                                                               >  1:2473712/1‑101 (MQ=255)
                       gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCt                                                                               <  1:174218/101‑1 (MQ=255)
                          ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTccc                                                                            >  2:3450246/1‑101 (MQ=255)
                           tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTcccc                                                                           >  2:5492601/1‑101 (MQ=255)
                             gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTcccccc                                                                         <  2:1012228/101‑1 (MQ=255)
                              cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCcg                                                                        <  2:5916546/101‑1 (MQ=255)
                              cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCcg                                                                        >  2:1605993/1‑101 (MQ=255)
                               ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCcgc                                                                       >  1:2665382/1‑101 (MQ=255)
                                gTCGACGGGCTGGTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCcgcg                                                                      <  2:1470264/101‑1 (MQ=255)
                                   gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCgggg                                                                   >  1:7095050/1‑101 (MQ=255)
                                       ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                               >  2:6798173/1‑101 (MQ=255)
                                       ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                               <  1:1146808/101‑1 (MQ=255)
                                         gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:901757/91‑1 (MQ=0)
                                         cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:2289802/101‑1 (MQ=255)
                                         aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:6628474/91‑1 (MQ=2)
                                          tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTTCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  2:4999435/101‑1 (MQ=255)
                                           cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:1042213/93‑1 (MQ=2)
                                            gccggtgatCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  2:6061953/93‑1 (MQ=2)
                                             tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:3627124/95‑1 (MQ=2)
                                             tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  2:5758856/7‑101 (MQ=2)
                                             tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:3203866/101‑1 (MQ=255)
                                              aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  2:7090388/1‑101 (MQ=255)
                                              aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  1:6665508/1‑101 (MQ=255)
                                              aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  1:523037/1‑101 (MQ=255)
                                               gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  2:2930367/5‑101 (MQ=2)
                                               aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.TCT.GC.TGGGCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  1:1792796/1‑101 (MQ=255)
                                                cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  2:93175/98‑1 (MQ=2)
                                                cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  2:5234102/4‑101 (MQ=2)
                                                cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  1:6312412/98‑1 (MQ=2)
                                                cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  2:1817711/101‑1 (MQ=255)
                                                 gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     >  2:5159559/1‑101 (MQ=255)
                                                 gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     <  1:4346602/101‑1 (MQ=255)
                                                 gtgtCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     <  2:2703251/101‑1 (MQ=255)
                                                  ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                    <  1:4188608/100‑2 (MQ=2)
                                                  cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                     >  1:2363647/2‑101 (MQ=2)
                                                   gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                                   >  1:2180544/1‑101 (MQ=255)
                                                   gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                   <  2:6965040/101‑3 (MQ=2)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                  <  2:2520841/101‑3 (MQ=2)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                  <  1:3392406/101‑3 (MQ=2)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGAAACCGG.CCC.G..GCGTCGCTACCCCCGCGGGGGCGTGGATCGAAaccccg                                                 >  1:3490078/1‑98 (MQ=2)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                 <  2:128664/101‑5 (MQ=2)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                 <  1:770332/101‑5 (MQ=2)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                               >  2:5370035/1‑96 (MQ=2)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TTCAGGCGG.CCC.GC.TCGTCGATCCCCCCGCGGGGGCGGGGATCGAAACCACCTTg                                              >  2:4585460/1‑101 (MQ=37)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                              <  1:3763561/101‑1 (MQ=255)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                              >  2:4767209/1‑101 (MQ=255)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcgg                                              >  2:1659927/1‑95 (MQ=2)
                                                         ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                             <  1:591387/101‑1 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                            >  1:6059472/1‑93 (MQ=0)
                                                          cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                            <  1:3011482/101‑10 (MQ=2)
                                                           ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                           <  1:5682331/101‑1 (MQ=255)
                                                           ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                           <  1:4897728/101‑1 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACATGGCTGTGGCGGCAGT.G.TCAGGTTTT.CCA.GCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccgccacgtct                                          <  2:3386594/101‑11 (MQ=0)
                                                             ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                         <  1:1991890/101‑1 (MQ=255)
                                                             ccGCGGGGGCGCGTCGCGAACCATCTCGCTGCCGTCGCTCG.CCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                         <  2:3305169/101‑1 (MQ=25)
                                                              cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                        <  1:4477286/101‑1 (MQ=255)
                                                               gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                       <  2:1900335/101‑1 (MQ=255)
                                                                 gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                     >  2:2405878/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                     <  2:3910860/101‑1 (MQ=255)
                                                                 gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                     <  2:483657/101‑1 (MQ=255)
                                                                  ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                    <  2:19898/101‑1 (MQ=255)
                                                                    ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                  >  2:4078252/1‑101 (MQ=255)
                                                                      cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                <  1:4116248/101‑1 (MQ=255)
                                                                       gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                               >  1:2901007/1‑101 (MQ=255)
                                                                       gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                               <  1:6404467/101‑1 (MQ=255)
                                                                       gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                               >  2:6228524/1‑101 (MQ=255)
                                                                        tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                              >  1:6846739/1‑101 (MQ=255)
                                                                         ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                             >  2:279060/1‑101 (MQ=255)
                                                                          gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                            >  2:6155441/1‑101 (MQ=255)
                                                                           aTCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                           >  1:6263921/1‑101 (MQ=255)
                                                                              gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                        <  1:6560217/101‑1 (MQ=255)
                                                                              gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                        >  2:2237029/1‑101 (MQ=255)
                                                                              gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                        >  1:4668579/1‑101 (MQ=255)
                                                                                aaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                      <  2:5636286/101‑1 (MQ=255)
                                                                                  cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                    >  1:2590641/1‑101 (MQ=255)
                                                                                   aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                   <  2:767788/101‑1 (MQ=255)
                                                                                    tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                  <  1:6146174/101‑1 (MQ=255)
                                                                                    tctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                  <  2:2629236/101‑1 (MQ=255)
                                                                                     ctcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt                 >  1:164769/1‑101 (MQ=255)
                                                                                      tcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                <  1:1926568/101‑1 (MQ=255)
                                                                                      tcGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                <  1:5364239/101‑1 (MQ=255)
                                                                                       cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg               >  1:142237/1‑101 (MQ=255)
                                                                                           gCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGGAGCGTCGCTcc           >  2:4351170/1‑101 (MQ=255)
                                                                                              gTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc        <  1:7021904/101‑1 (MQ=255)
                                                                                               tCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc       <  1:5493327/101‑1 (MQ=255)
                                                                                                gccgcG.TCCAGCCGG.CCT.GC.TCGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcg      <  2:3128355/97‑1 (MQ=255)
                                                                                                 gCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc     >  1:4035712/1‑101 (MQ=255)
                                                                                                    |                                                                                                        
GGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGG  >  NC_014034/1395449‑1395649

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG