BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,550	G→T	10.6%	intergenic (+362/‑569)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,550	0	G→T	10.6%	142.0 ‑0.6 ‑1.3	507	intergenic (+362/‑569)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (28/25): ref base (212/233): total (244/263)
Fisher's exact test strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.81e-02

Only 100 of 532 total aligned reads displayed.


GGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGG  >  NC_014034/1395450‑1395651


 ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.t                                                                                                         >  1:6088906/1‑101 (MQ=255)


 ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGCTGCCGTCGCTCgg                                                                                                          >  1:5701936/1‑100 (MQ=255)


    gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                      <  2:6398578/101‑1 (MQ=255)


     tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                                     >  2:4308110/1‑101 (MQ=255)


        aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCg                                                                                                  <  1:1931453/101‑1 (MQ=255)


           gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c.c                                                                                             <  1:6728613/101‑1 (MQ=255)


            aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.Ct                                                                                            <  1:1241700/101‑1 (MQ=255)


            aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.Ct                                                                                            <  1:1576947/101‑1 (MQ=255)


            aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.Ct                                                                                            >  1:1817775/1‑101 (MQ=255)


             aaCATCGCCGCTTTGGCGGTCGACTGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.g                                                                                          <  2:1813697/101‑1 (MQ=255)


              aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.Gc                                                                                         <  2:4771977/101‑1 (MQ=255)


               cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTGGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCt                                                                                        <  2:2443034/101‑1 (MQ=255)


                aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCtc                                                                                       <  2:2364148/101‑1 (MQ=255)


                   tctgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCtcgtc                                                                                    <  1:6156252/98‑1 (MQ=255)


                   gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCtcgtc                                                                                    <  1:3918751/101‑1 (MQ=255)


                   gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GGtcgtc                                                                                    >  2:2564652/1‑101 (MQ=255)


                      gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCt                                                                                 <  2:6306192/101‑1 (MQ=255)


                      gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCt                                                                                 <  1:174218/101‑1 (MQ=255)


                          tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTcccc                                                                             >  2:5492601/1‑101 (MQ=255)


                            gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTcccccc                                                                           >  2:1846228/1‑101 (MQ=255)


                             cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCcg                                                                          <  2:5916546/101‑1 (MQ=255)


                                     aGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCg                                                                  >  2:4053940/1‑101 (MQ=255)


                                      ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                                 <  1:1146808/101‑1 (MQ=255)


                                      ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                                 >  2:6798173/1‑101 (MQ=255)


                                       gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                >  1:4622481/1‑101 (MQ=255)


                                       gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                >  2:217766/1‑101 (MQ=255)


                                       gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGCCGCTCCCCCCGCGGGGGCgcg                                                                >  2:1114043/1‑99 (MQ=255)


                                        cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  1:789563/101‑1 (MQ=255)


                                        agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.C.CC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  2:4357589/91‑1 (MQ=0)


                                        aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  2:6628474/91‑1 (MQ=2)


                                        aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                                               <  2:1053672/91‑1 (MQ=2)


                                        aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGG.CACA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                                               >  2:1944692/11‑101 (MQ=2)


                                         tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTTCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  2:4999435/101‑1 (MQ=255)


                                          ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  1:6973718/9‑101 (MQ=2)


                                          ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  1:2375785/93‑1 (MQ=2)


                                          cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  1:1042213/93‑1 (MQ=2)


                                           gccggtgatCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  2:6061953/93‑1 (MQ=2)


                                           ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  2:3397077/94‑1 (MQ=2)


                                            tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           >  2:5758856/7‑101 (MQ=2)


                                            tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           >  1:7128520/7‑101 (MQ=2)


                                             aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          >  1:6272257/1‑101 (MQ=255)


                                             aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          >  2:7090388/1‑101 (MQ=255)


                                             aaCGTGCCCCTCCCCCCGCGGGGGCGTGGAAAGTAACGTCTCGGTGCCGTGGCGCG.ACCCGCCAG.C.CT.GCACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          <  1:7034951/101‑1 (MQ=21)


                                              ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  1:2595/5‑101 (MQ=2)


                                              gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  2:2843722/5‑101 (MQ=2)


                                              aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  1:920314/101‑1 (MQ=255)


                                              aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  1:3450370/101‑1 (MQ=255)


                                               cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.G.CTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  2:93175/98‑1 (MQ=2)


                                               cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  2:5234102/4‑101 (MQ=2)


                                                tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                       <  1:2939934/99‑1 (MQ=2)


                                                tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                       <  2:2756846/99‑1 (MQ=2)


                                                gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                       >  2:5159559/1‑101 (MQ=255)


                                                gcgtCGCTCCCCCCGCGGCGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.G.CTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  1:3693752/3‑101 (MQ=2)


                                                ccgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                       <  2:3045167/99‑1 (MQ=2)


                                                 ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                      <  1:6566372/100‑1 (MQ=255)


                                                 ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                      <  1:6439162/100‑2 (MQ=2)


                                                 ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                      <  2:3226790/100‑2 (MQ=2)


                                                 cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.G.CTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                       >  1:4541045/2‑101 (MQ=2)


                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                                     >  2:3680484/1‑101 (MQ=255)


                                                  gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                     <  2:6965040/101‑3 (MQ=2)


                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                    <  1:5571122/101‑1 (MQ=255)


                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                   >  1:2671184/1‑101 (MQ=255)


                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                   >  1:3514241/1‑97 (MQ=2)


                                                     gccccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.C.CC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                  <  1:4244025/98‑5 (MQ=2)


                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                                  >  1:2517397/1‑101 (MQ=255)


                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                                  >  1:4163831/1‑101 (MQ=255)


                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                                 >  1:5258459/1‑95 (MQ=2)


                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.C.CC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                                 >  2:5370035/1‑96 (MQ=2)


                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                <  1:406586/101‑8 (MQ=2)


                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                >  2:4165219/1‑94 (MQ=2)


                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGACGTGGATCGAAactgcgtgc                                                >  1:4357811/1‑94 (MQ=2)


                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.C.CC.G.GCGGCGCTCCCCCCGCGGGGGCGTGGATCGGAaccccgcgg                                                >  1:201884/1‑95 (MQ=2)


                                                        ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCGCCCCCCCCGGGGGCGGGGATCGAAactgggtgcg                                               >  1:2290816/1‑93 (MQ=2)


                                                          ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                             >  1:6331641/1‑101 (MQ=255)


                                                          ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                             >  2:4489887/1‑91 (MQ=2)


                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                        >  1:5999843/1‑101 (MQ=255)


                                                                gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                       <  2:2064902/101‑1 (MQ=255)


                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                  <  1:5388419/101‑1 (MQ=255)


                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                  <  1:4767449/101‑1 (MQ=255)


                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACCCCCTGGCGGATTGCCACttcg                                 >  1:3284432/1‑99 (MQ=38)


                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                 >  2:6228524/1‑101 (MQ=255)


                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                 <  1:3025115/101‑1 (MQ=255)


                                                                       tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                >  2:1503706/1‑101 (MQ=255)


                                                                         gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                              >  2:6155441/1‑101 (MQ=255)


                                                                            cGAAACATCGCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg                           <  1:3800053/101‑1 (MQ=255)


                                                                               aaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                        <  2:5636286/101‑1 (MQ=255)


                                                                                aCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa                       >  2:128720/1‑101 (MQ=255)


                                                                                 cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATAGTCACTTTCGCGGGGCGAAg                      >  1:5631713/1‑101 (MQ=255)


                                                                                  aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                     <  1:51110/101‑1 (MQ=255)


                                                                                  aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                     <  2:1708703/101‑1 (MQ=255)


                                                                                  aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                     <  2:767788/101‑1 (MQ=255)


                                                                                   tctcGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                    <  2:2665286/101‑1 (MQ=255)


                                                                                   tctcGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                    <  1:218681/101‑1 (MQ=255)


                                                                                    ctcGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGt                   >  1:164769/1‑101 (MQ=255)


                                                                                      cGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                 >  2:162330/1‑101 (MQ=255)


                                                                                      cGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                 >  1:142237/1‑101 (MQ=255)


                                                                                        gTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt               <  1:2847139/101‑1 (MQ=255)


                                                                                           ccGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGCAGCGCCGCTccc            >  2:4853174/1‑101 (MQ=255)


                                                                                             gTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccccc          <  2:4640409/101‑1 (MQ=255)


                                                                                                gCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc       >  2:3332906/1‑101 (MQ=255)


GGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGG  >  NC_014034/1395450‑1395651

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG