BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,552	C→G	30.9%	intergenic (+364/‑567)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,552	0	C→G	30.9%	0.0 ‑0.5 ‑0.3	506	intergenic (+364/‑567)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (71/80): ref base (171/174): total (247/259)
Fisher's exact test strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.08e-01

Only 100 of 537 total aligned reads displayed.


CGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395453‑1395652


cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.Tcc                                                                                                        <  1:7095824/101‑1 (MQ=255)


 gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCa                                                                                                       <  2:6398578/101‑1 (MQ=255)


  tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAg                                                                                                      >  1:4167159/1‑101 (MQ=255)


        gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c.c                                                                                              >  2:4270055/1‑101 (MQ=255)


        gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c.c                                                                                              <  1:5350350/101‑1 (MQ=255)


         aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.Ct                                                                                             <  1:1241700/101‑1 (MQ=255)


         aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.Ct                                                                                             <  2:5671973/101‑1 (MQ=255)


         aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGATCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.Ct                                                                                             >  2:3388313/1‑101 (MQ=255)


          aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.g                                                                                           <  1:2240420/101‑1 (MQ=255)


             agcgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCtc                                                                                        <  2:4670891/99‑1 (MQ=255)


             aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCtc                                                                                        >  2:1107579/1‑101 (MQ=255)


               cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCtcgt                                                                                      >  2:2540983/1‑101 (MQ=255)


                   gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCtcgtcaga                                                                                  >  2:6155933/1‑98 (MQ=255)


                   gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCt                                                                                  <  2:6306192/101‑1 (MQ=255)


                   gcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCt                                                                                  >  2:4336335/1‑101 (MQ=255)


                    cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTc                                                                                 >  1:2404489/1‑101 (MQ=255)


                         gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTcccccc                                                                            >  2:1846228/1‑101 (MQ=255)


                          cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCACCCGG.C.CT.GCTCGTCGCTCCCCCcg                                                                           >  1:1464763/1‑101 (MQ=255)


                               gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCgggg                                                                      >  2:5378170/1‑101 (MQ=255)


                                 cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGc                                                                    <  1:6698532/101‑1 (MQ=255)


                                   ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                                  >  1:4558014/1‑101 (MQ=255)


                                   ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                                  >  2:6798173/1‑101 (MQ=255)


                                   ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                                  <  1:1146808/101‑1 (MQ=255)


                                     agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.C.CC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                <  1:2524607/91‑1 (MQ=0)


                                     aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGG.CACA.GGATGTCGCTCCCCCCGCGGGGGCGTgg                                                                >  2:1944692/11‑101 (MQ=2)


                                      tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:2766275/101‑1 (MQ=255)


                                      tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:4425985/101‑1 (MQ=255)


                                       ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  2:2983831/9‑101 (MQ=2)


                                       ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  2:3156785/101‑1 (MQ=255)


                                       ggTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:4069645/101‑1 (MQ=255)


                                       cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  2:2739487/93‑1 (MQ=2)


                                       cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:1042213/93‑1 (MQ=2)


                                        gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             >  1:5587694/8‑101 (MQ=2)


                                        gccggtgatCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:6061953/93‑1 (MQ=2)


                                        ctcgcacgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:7026419/94‑1 (MQ=2)


                                         tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  1:305985/95‑1 (MQ=2)


                                         taccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.G.CTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:1040140/95‑1 (MQ=2)


                                         tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  1:3809706/101‑1 (MQ=255)


                                          cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           >  2:1762418/6‑101 (MQ=2)


                                          aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           >  1:2629332/1‑101 (MQ=255)


                                           ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:3328944/97‑1 (MQ=2)


                                           gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  1:5190702/97‑1 (MQ=2)


                                           aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:2661266/101‑1 (MQ=255)


                                            gtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  1:5866552/4‑101 (MQ=2)


                                            cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.G.CTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:93175/98‑1 (MQ=2)


                                            cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  2:7080361/101‑1 (MQ=255)


                                            cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:4910321/1‑101 (MQ=255)


                                             tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                        >  1:159788/3‑101 (MQ=2)


                                             cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.C.CC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                        <  1:1847456/99‑1 (MQ=2)


                                             acgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                        >  2:5278950/3‑101 (MQ=2)


                                              tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                       <  1:3529605/101‑1 (MQ=255)


                                              cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                       >  2:1613089/2‑100 (MQ=2)


                                               gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                                      >  1:1655920/1‑101 (MQ=255)


                                               gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAaccc                                                      >  2:6615877/1‑100 (MQ=255)


                                               gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGACactg                                                      >  2:549264/1‑99 (MQ=2)


                                               gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                      <  2:6965040/101‑3 (MQ=2)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                    <  1:770332/101‑5 (MQ=2)


                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                    <  1:4870860/101‑5 (MQ=2)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATccgaactgagt                                                   >  2:5012884/1‑91 (MQ=2)


                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                                   >  2:204731/1‑96 (MQ=2)


                                                    tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TTCAGGCGG.C.CC.GCTCGTCGATCCCCCCGCGGGGGCGGGGATCGAAACCACCTTg                                                 >  2:4585460/1‑101 (MQ=37)


                                                    tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGAC.CC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                 >  1:233886/1‑94 (MQ=2)


                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                 <  1:1762482/101‑8 (MQ=2)


                                                     ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                >  2:5162555/1‑93 (MQ=2)


                                                     ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAA.CA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                >  2:3625954/1‑93 (MQ=2)


                                                      cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.C.CC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                               >  1:6059472/1‑93 (MQ=0)


                                                       ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGGCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                              <  1:6328157/101‑1 (MQ=255)


                                                          cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                           <  1:3388437/101‑1 (MQ=255)


                                                           gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                          >  2:5802507/1‑101 (MQ=255)


                                                           gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                          <  1:1932213/101‑1 (MQ=255)


                                                            cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                         <  2:5927532/101‑1 (MQ=255)


                                                             gggggCGTGGATCGAAACCTCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                        >  2:2804551/1‑101 (MQ=255)


                                                              ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                       >  2:3924870/1‑101 (MQ=255)


                                                               gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                      >  1:347809/1‑101 (MQ=255)


                                                                 gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                    <  1:2073807/101‑1 (MQ=255)


                                                                   gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                  >  2:6228524/1‑101 (MQ=255)


                                                                   gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                  <  1:6404467/101‑1 (MQ=255)


                                                                    tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                 >  1:6846739/1‑101 (MQ=255)


                                                                     ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                                >  1:2867793/1‑101 (MQ=255)


                                                                      gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                               >  1:465423/1‑101 (MQ=255)


                                                                       aTCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                              >  1:6263921/1‑101 (MQ=255)


                                                                          gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                           >  1:916201/1‑101 (MQ=255)


                                                                          gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                           <  1:6560217/101‑1 (MQ=255)


                                                                           aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                          >  2:2427966/1‑101 (MQ=255)


                                                                             aCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa                        <  1:5637064/101‑1 (MQ=255)


                                                                             aCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa                        >  2:128720/1‑101 (MQ=255)


                                                                              cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                       >  2:4464570/1‑101 (MQ=255)


                                                                               aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                      <  2:1708703/101‑1 (MQ=255)


                                                                               aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                      <  2:767788/101‑1 (MQ=255)


                                                                                tctcGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                     <  1:6146174/101‑1 (MQ=255)


                                                                                  tcGGTGCCGTCGCTCG.TCCAGCNGN.C.NT.GNNCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                   <  1:2965011/101‑1 (MQ=255)


                                                                                       gCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc              <  1:54059/101‑1 (MQ=255)


                                                                                        ccGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc             >  2:2177386/1‑101 (MQ=255)


                                                                                            cGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcg         <  1:4297956/101‑1 (MQ=255)


                                                                                              cTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgcg       >  2:2332692/1‑101 (MQ=255)


                                                                                                cG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCggg     >  2:1502684/1‑101 (MQ=255)


                                                                                                 g.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg    >  2:4079752/1‑101 (MQ=255)


                                                                                                    ccAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGc  <  1:3925045/101‑1 (MQ=255)


                                                                                                    ccAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGc  >  1:3714188/1‑101 (MQ=255)


                                                                                                    ccAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGc  >  2:4809057/1‑101 (MQ=255)


CGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.C.CT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395453‑1395652

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG