Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,557 C→T 13.8% intergenic (+369/‑562) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5570C→T13.8% 185.2 ‑1.2 ‑0.5507intergenic (+369/‑562)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (30/40):  ref base (218/219):  total (248/259)
Fisher's exact test strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.44e-01

Only 100 of 530 total aligned reads displayed.

GATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395457‑1395657
                                                                                                     |                                                                                                       
gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGcc                                                                                                         <  2:2126430/101‑1 (MQ=255)
gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGcc                                                                                                         <  1:3896971/101‑1 (MQ=255)
   cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.c                                                                                                     >  2:5541647/1‑101 (MQ=255)
     aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                                   <  1:1241700/101‑1 (MQ=255)
      aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                                 <  1:2240420/101‑1 (MQ=255)
      aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.g                                                                                                 >  2:5682041/1‑101 (MQ=255)
       aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.Gc                                                                                                <  2:4771977/101‑1 (MQ=255)
         aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tc                                                                                             >  2:192184/1‑101 (MQ=255)
          tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tcg                                                                                            <  1:644849/101‑1 (MQ=255)
           cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCTGGGCCGTCGCTCG.CCCAACCGG.CCT.GG.tcgt                                                                                           >  1:1917222/1‑101 (MQ=37)
            tctgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tcgtc                                                                                          <  1:6156252/98‑1 (MQ=255)
            gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.tcgtc                                                                                          <  2:1277977/101‑1 (MQ=255)
             ccgcTTTGGCGGTCGTCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCTG.CCT.GC.tcgtcg                                                                                         <  2:5201031/101‑1 (MQ=255)
                  ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTccc                                                                                    >  2:3450246/1‑101 (MQ=255)
                  ttGGCGGTCGACAGGCTGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCCGCCGG.CCT.GC.TCGTCGCTccc                                                                                    >  2:2725445/1‑101 (MQ=255)
                    ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTccccc                                                                                  >  2:2457311/1‑101 (MQ=255)
                     gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTcccccc                                                                                 <  2:5440726/101‑1 (MQ=255)
                     gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTcccccc                                                                                 <  2:1012228/101‑1 (MQ=255)
                        gTCGACGGGCTGGTAACGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCcgcg                                                                              <  2:1470264/101‑1 (MQ=255)
                        gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTGGCTCCCCCcgcg                                                                              <  1:5087399/101‑1 (MQ=255)
                          cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCggg                                                                            >  2:1822824/1‑101 (MQ=255)
                               ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                                       <  2:3134303/101‑1 (MQ=255)
                               ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                                       <  1:1146808/101‑1 (MQ=255)
                               ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGt                                                                       >  2:6798173/1‑101 (MQ=255)
                                 gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  2:6214746/91‑1 (MQ=0)
                                 gccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  1:901757/91‑1 (MQ=0)
                                 aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  2:6628474/91‑1 (MQ=2)
                                  aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGa                                                                    <  1:238321/92‑1 (MQ=0)
                                   ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   >  2:2983831/9‑101 (MQ=2)
                                   cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCTGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  1:4205476/93‑1 (MQ=2)
                                   cctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  1:1042213/93‑1 (MQ=2)
                                    ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:2614646/94‑1 (MQ=2)
                                     taccgcgtCGCTCCCCCCGCTGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:5593017/95‑1 (MQ=2)
                                     ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  2:6378271/95‑1 (MQ=2)
                                     ccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  1:2334469/95‑1 (MQ=2)
                                      cgcacggcGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                <  1:5350875/94‑1 (MQ=2)
                                      aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                >  1:6272257/1‑101 (MQ=255)
                                      aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                >  2:7090388/1‑101 (MQ=255)
                                       gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  1:6071332/5‑101 (MQ=2)
                                       aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               <  2:2661266/101‑1 (MQ=255)
                                        cgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               <  2:93175/98‑1 (MQ=2)
                                         tggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             >  1:159788/3‑101 (MQ=2)
                                         gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             >  1:6999408/1‑101 (MQ=255)
                                         gtgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             <  1:4346602/101‑1 (MQ=255)
                                          tgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                            >  1:5244157/1‑101 (MQ=255)
                                          ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                            <  1:5989108/100‑1 (MQ=2)
                                           gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                                           >  1:1655920/1‑101 (MQ=255)
                                           gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                           <  2:6965040/101‑3 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                         <  1:770332/101‑5 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                         >  1:3514241/1‑97 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                         >  2:1570366/1‑97 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCt                                                        >  1:2517397/1‑101 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgg                                                        >  1:1432997/1‑96 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                                        >  2:4196589/1‑96 (MQ=2)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G..GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                                       >  2:5370035/1‑96 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                      <  1:3763561/101‑1 (MQ=255)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                      <  1:1762482/101‑8 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                                     >  1:4608792/1‑101 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                                     >  2:1102029/1‑101 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                     <  1:3075950/101‑9 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                     >  2:1653188/1‑93 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GG.ATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                     <  2:5991747/101‑9 (MQ=2)
                                                   ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                                   <  2:1745152/101‑1 (MQ=255)
                                                   ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                                   <  1:5682331/101‑1 (MQ=255)
                                                    cccGCGGGGGCGTGGATCGAAACATGGCTGTGGCGGCAGT.G.TCAGGTTTT.CCA.GCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccgccacgtct                                                  <  2:3386594/101‑11 (MQ=0)
                                                     ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                                 >  2:6268553/1‑101 (MQ=255)
                                                      cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                                <  1:3388437/101‑1 (MQ=255)
                                                      cgcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                                <  1:4477286/101‑1 (MQ=255)
                                                         gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                             >  2:2405878/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                             <  2:2798846/101‑1 (MQ=255)
                                                         gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                             <  1:5541937/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                            >  2:3924870/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGACACCACCTTGCGGATTGTCaat                                          >  2:4659673/1‑99 (MQ=255)
                                                             gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                         <  1:2073807/101‑1 (MQ=255)
                                                              cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                        <  1:4767449/101‑1 (MQ=255)
                                                              cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                        <  1:6359709/101‑1 (MQ=255)
                                                              cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                        <  1:4116248/101‑1 (MQ=255)
                                                               gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                       >  2:201579/1‑101 (MQ=255)
                                                                tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                      >  1:6846739/1‑101 (MQ=255)
                                                                  gATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                                    >  2:6155441/1‑101 (MQ=255)
                                                                   aTCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                                   >  2:4061178/1‑101 (MQ=255)
                                                                      gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                                >  2:5121289/1‑101 (MQ=255)
                                                                      gAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                                >  2:2237029/1‑101 (MQ=255)
                                                                        accATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                              <  2:4574962/99‑1 (MQ=255)
                                                                           aTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                           <  2:767788/101‑1 (MQ=255)
                                                                              tcGGTGCCGTCGCTCG.TCCAGCNGN.CNT.GN.NCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                        <  1:2965011/101‑1 (MQ=255)
                                                                               cGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                       <  2:5875549/101‑1 (MQ=255)
                                                                                 gTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt                     <  1:3680633/101‑1 (MQ=255)
                                                                                 gTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt                     <  1:2847139/101‑1 (MQ=255)
                                                                                   gCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcc                   <  2:4334451/101‑1 (MQ=255)
                                                                                    ccGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGCAGCGCCGCTccc                  >  2:4853174/1‑101 (MQ=255)
                                                                                       tCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc               <  1:1935954/101‑1 (MQ=255)
                                                                                       gcGCCCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc               <  2:4425603/100‑1 (MQ=255)
                                                                                        atcTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcg              <  1:6560224/99‑1 (MQ=255)
                                                                                         gCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc             >  1:4035712/1‑101 (MQ=255)
                                                                                                ccAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGc       >  2:4967936/1‑101 (MQ=255)
                                                                                                   gCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTg    <  1:6717906/101‑1 (MQ=255)
                                                                                                    ccGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg   <  1:7090762/101‑1 (MQ=255)
                                                                                                    ccGG.CCC.GC.CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg   <  2:3290520/101‑1 (MQ=255)
                                                                                                     cGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGa  <  1:2688015/101‑1 (MQ=255)
                                                                                                     |                                                                                                       
GATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GC.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395457‑1395657

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG