Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,685 C→A 5.6% intergenic (+497/‑434) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6850C→A 5.6% 38.3 0.0 ‑0.0374intergenic (+497/‑434)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/9):  ref base (193/159):  total (206/168)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.02e-01

Only 100 of 429 total aligned reads displayed.

GCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395585‑1395785
                                                                                                       |                                                                                                     
gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..Gc                                                                                                       <  2:4445359/101‑1 (MQ=255)
  gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAg                                                                                                     >  1:116810/1‑101 (MQ=255)
   tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGt                                                                                                    >  1:3016505/1‑101 (MQ=255)
    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGtt                                                                                                   >  2:2118791/1‑101 (MQ=255)
    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGtt                                                                                                   >  1:2364458/1‑101 (MQ=255)
     gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTc                                                                                                  <  1:1351721/101‑1 (MQ=255)
     gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTc                                                                                                  <  1:2178424/101‑1 (MQ=255)
      aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCg                                                                                                 <  2:2617516/101‑1 (MQ=255)
      aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCg                                                                                                 <  1:1870073/101‑1 (MQ=255)
       tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGCAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTTCATGTAGTCGGCG.G..GCAGTTCGa                                                                                                >  2:832486/1‑101 (MQ=255)
       tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGa                                                                                                >  1:4743419/1‑101 (MQ=255)
        cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCTAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.g                                                                                              <  1:6228843/101‑1 (MQ=255)
         gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.Gc                                                                                             <  1:6592525/101‑1 (MQ=255)
           aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCg                                                                                           <  1:6678770/101‑1 (MQ=255)
           aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCg                                                                                           >  2:4010374/1‑101 (MQ=255)
            tcagtcatGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGc                                                                                          >  2:3267065/8‑101 (MQ=255)
            accaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGc                                                                                          >  1:5997708/1‑101 (MQ=255)
             ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCa                                                                                         >  2:6783074/1‑101 (MQ=255)
              caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAg                                                                                        >  2:3810991/1‑101 (MQ=255)
                ccTTGCGGATTGTCACTGGCGCGGGGCAAATCGTCGCACCCCACGCGGGGACGAGAATCGAAACATCTGAATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTc                                                                                      <  2:1269876/101‑1 (MQ=255)
                 cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCg                                                                                     >  2:4309167/1‑101 (MQ=255)
                   tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCt                                                                                   <  2:1808573/101‑1 (MQ=255)
                    gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                  <  1:4569561/101‑1 (MQ=255)
                    gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                  <  2:2548758/101‑1 (MQ=255)
                    gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTc                                                                                  >  1:5771691/1‑101 (MQ=255)
                     gggATTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcc                                                                                 <  2:5892530/100‑1 (MQ=255)
                     cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcc                                                                                 >  2:1004178/1‑101 (MQ=255)
                        aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGG.G..GCAGTTCGA.GCCGCAGTCGCTccccc                                                                              >  1:4977694/1‑101 (MQ=255)
                        aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTccccc                                                                              >  2:1520442/1‑101 (MQ=255)
                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                             <  2:3452774/101‑1 (MQ=255)
                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                             <  2:476159/101‑1 (MQ=255)
                         ttGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTcccccc                                                                             <  2:1891281/101‑1 (MQ=255)
                          tGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                            <  2:5631423/101‑1 (MQ=255)
                           gTCGCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                           >  1:6825014/1‑101 (MQ=255)
                           gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                           >  2:6723088/1‑101 (MQ=255)
                           gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                                           <  2:6088594/101‑1 (MQ=255)
                             cACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgg                                                                         >  1:6461341/1‑101 (MQ=255)
                               cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGTgggg                                                                       >  1:3200085/1‑101 (MQ=255)
                               cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                                       >  2:1408783/1‑101 (MQ=255)
                                 ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                                     >  1:1844897/1‑101 (MQ=255)
                                    tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                                   >  1:2251706/11‑101 (MQ=2)
                                     ggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                  >  2:1700774/10‑101 (MQ=2)
                                      ggggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                >  1:2488067/1‑101 (MQ=255)
                                      gggccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:1446459/93‑1 (MQ=2)
                                       gggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  2:2771258/101‑1 (MQ=255)
                                        tcactcgagccGCTCCCCCCGGGGGGGCGTGGTGCAAAACATCGGCTTGTCCGCTTCGAA..CCTGGTCGC.GTCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  2:2322139/91‑1 (MQ=2)
                                        ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              >  1:5195755/1‑101 (MQ=255)
                                          cGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  1:1221912/1‑101 (MQ=255)
                                          cGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  1:436388/101‑1 (MQ=255)
                                           gctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  2:2845132/4‑101 (MQ=2)
                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  2:6177682/1‑101 (MQ=255)
                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  2:1940605/1‑101 (MQ=255)
                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  1:105771/1‑101 (MQ=255)
                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  1:673089/1‑101 (MQ=255)
                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  1:461645/1‑101 (MQ=255)
                                            aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          >  2:5418038/1‑101 (MQ=255)
                                             tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GC.GAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          >  2:3942573/2‑101 (MQ=2)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGTG.GCAGGTTTTCCA.GCGGC.GTCGCTCCCCCCGCGGGGGCGTGGAGCGAAAcc                                                        >  2:6877772/1‑100 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                                      <  1:6899952/101‑1 (MQ=255)
                                                gTCGCCCCCCTCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACTgg                                                      <  1:5082973/101‑1 (MQ=255)
                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                                      <  1:2600059/101‑1 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGCGGATCGGAACTGGTAt                                                   >  2:5088065/1‑101 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACACCTGCATGTAGTGGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                                   <  2:5748848/101‑1 (MQ=255)
                                                     tCCCCCCGGGGGGGCGTGGATCGAAACATCTGCATGTAGCCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                 <  1:832510/101‑1 (MQ=255)
                                                       cccccGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                                               <  2:523025/101‑1 (MQ=255)
                                                         cccGCGGGGGCGTGGACCGAACCACCTGTTTGTAGCCGCCG.G..CAGGTACGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                                             <  1:976290/101‑1 (MQ=21)
                                                          ccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTg                                            >  2:1213817/1‑101 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                                           <  2:4579664/101‑1 (MQ=255)
                                                              gggggCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                        >  2:4899301/1‑101 (MQ=255)
                                                                gggGGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGGTATGGATCTGGCGCaa                                      >  1:5621309/1‑101 (MQ=255)
                                                                    gTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                  >  1:4252927/1‑101 (MQ=255)
                                                                    gTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                  >  1:2791315/1‑101 (MQ=255)
                                                                     gggATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                 <  2:6298452/100‑1 (MQ=255)
                                                                       gATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATGGAAACTGGTATGGATCTGGCGCAAACGGaaa                               >  1:4557292/1‑101 (MQ=255)
                                                                        acaaaacgctCTGCCTGTCGTGGCGG.G..GAAGTGGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                              <  2:1640120/92‑1 (MQ=21)
                                                                          cGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                            >  2:6994369/1‑101 (MQ=255)
                                                                             aaCATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                         <  1:3987715/101‑1 (MQ=255)
                                                                             aaCATCTGCATGTAGGCGGGG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                         <  1:1107614/101‑1 (MQ=255)
                                                                             aaCATCTGCATGTAGGCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgt                         <  1:4309389/101‑1 (MQ=255)
                                                                              aCATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtc                        <  2:2916430/101‑1 (MQ=255)
                                                                               cATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg                       <  1:6327812/101‑1 (MQ=255)
                                                                                aTCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTNTGGATCTGGCGCAAACGGAAAGTCTgtcgt                      >  1:1984913/1‑101 (MQ=255)
                                                                                aTCTGCATGTAGGCGGGG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt                      <  1:6077143/101‑1 (MQ=255)
                                                                                  cTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtcc                    >  1:1668764/1‑100 (MQ=255)
                                                                                  cTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGGCTGtcgtcg                    >  2:4103895/1‑101 (MQ=255)
                                                                                  cTGCATGGAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                    <  1:5368326/101‑1 (MQ=255)
                                                                                  cTGCAGGTAGGCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg                    <  2:3415184/101‑1 (MQ=255)
                                                                                      aTGTGGGCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcc                <  1:670441/101‑1 (MQ=255)
                                                                                         tAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccc             <  2:6461011/101‑1 (MQ=255)
                                                                                         tAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccc             >  1:3595339/1‑101 (MQ=255)
                                                                                          agccgggg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc            <  2:6594548/98‑1 (MQ=255)
                                                                                           ggcggcg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg           <  2:4673463/99‑1 (MQ=255)
                                                                                           gTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCcg           >  1:387934/1‑101 (MQ=255)
                                                                                              ggcg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg        >  2:5031797/1‑101 (MQ=255)
                                                                                                cg.g..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgggg      >  2:3157127/1‑101 (MQ=255)
                                                                                                 g.g..gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg     >  2:3620416/1‑101 (MQ=255)
                                                                                                 g.g..gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggggg     >  1:3264346/1‑101 (MQ=255)
                                                                                                   g..gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGc    <  1:1536426/101‑1 (MQ=255)
                                                                                                      gCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCg   <  1:4054116/101‑1 (MQ=255)
                                                                                                       cAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGt  <  1:3440553/101‑1 (MQ=255)
                                                                                                       |                                                                                                     
GCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G..GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395585‑1395785

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG