Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,746 G→C 14.6% intergenic (+558/‑373) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7460G→C14.6% 176.8 ‑0.6 ‑0.0501intergenic (+558/‑373)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (33/40):  ref base (211/217):  total (244/257)
Fisher's exact test strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 524 total aligned reads displayed.

CGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCG  >  NC_014034/1395646‑1395846
                                                                                                       |                                                                                                        
cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..gcg                                                                                                          >  2:6053276/1‑101 (MQ=255)
 gggggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..gcgc                                                                                                         >  2:4899301/1‑101 (MQ=255)
     gggtagATCGAAACCTCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAAc                                                                                                     <  2:1221864/96‑1 (MQ=255)
     gCGGGGATCGAAACATCTGCAGGTAGTCGGCGGGCAGTGCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAAc                                                                                                     <  2:2029938/101‑1 (MQ=255)
         ggATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..aa                                                                                               <  2:1831479/101‑1 (MQ=255)
         ggATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..aa                                                                                               <  1:4514480/101‑1 (MQ=255)
         ggATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..aa                                                                                               >  2:4091292/1‑101 (MQ=255)
             cGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTc                                                                                           <  1:1316622/101‑1 (MQ=255)
                 aCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tc                                                                                     <  2:2916430/101‑1 (MQ=255)
                   aTCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                                   >  1:6627045/1‑101 (MQ=255)
                    tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcgtc                                                                                  <  1:2349569/101‑1 (MQ=255)
                     cggCATGTAGCCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.tcgtcg                                                                                 <  2:3642552/99‑1 (MQ=255)
                     cTGCAGGTAGGCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.tcgtcg                                                                                 <  2:3415184/101‑1 (MQ=255)
                       gCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tagatcgg                                                                               >  1:1195874/1‑94 (MQ=255)
                       gCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                               >  2:1930570/1‑101 (MQ=255)
                        cATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTc                                                                              <  2:3992258/101‑1 (MQ=255)
                         aTGTGGGCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcc                                                                             <  1:670441/101‑1 (MQ=255)
                          tGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccc                                                                            <  1:1896641/101‑1 (MQ=255)
                          tGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccc                                                                            >  1:5242305/1‑101 (MQ=255)
                            tAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccccc                                                                          <  2:6461011/101‑1 (MQ=255)
                              gTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcg                                                                        >  1:387934/1‑101 (MQ=255)
                                  gcggGCAGTGCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggg                                                                    <  2:6271934/101‑1 (MQ=255)
                                   cggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgggg                                                                   >  2:3157127/1‑101 (MQ=255)
                                    gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggggg                                                                  >  1:3264346/1‑101 (MQ=255)
                                    gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggggg                                                                  >  2:2806136/1‑101 (MQ=255)
                                       cAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGt                                                               >  1:4569271/1‑101 (MQ=255)
                                            cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  1:2678117/1‑101 (MQ=255)
                                            cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  1:602253/1‑101 (MQ=255)
                                             gAGCCGCAGTCGCTCCGCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:4821515/1‑101 (MQ=255)
                                             gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  2:4187063/1‑101 (MQ=255)
                                             gAGCCGCAGTCGCTCCCCCCGCGGGGGCGCGGGTCGAAACT.GGTAGGGATCTG..GCGCAAACGG..CAAGGCT.G.CCGTCGCTCCCCCCGCGGGGGGGTGGATCg                                                         >  2:2375224/1‑101 (MQ=37)
                                              cgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  2:5342170/94‑1 (MQ=0)
                                              agattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  2:707062/6‑101 (MQ=2)
                                               gtgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  2:2132830/7‑101 (MQ=0)
                                               gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  1:4204290/5‑101 (MQ=2)
                                                 gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  1:3948060/5‑101 (MQ=2)
                                                   cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  2:6142818/101‑1 (MQ=2)
                                                   cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  2:1128454/101‑1 (MQ=2)
                                                   cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  2:4640535/1‑101 (MQ=2)
                                                   cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                   <  1:2541077/101‑1 (MQ=255)
                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:2217488/101‑1 (MQ=2)
                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:371749/101‑1 (MQ=2)
                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:5130720/101‑1 (MQ=2)
                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                  <  2:5771389/101‑1 (MQ=255)
                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                  <  2:1521156/101‑1 (MQ=255)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                 <  2:6582176/101‑1 (MQ=255)
                                                     gTCGCGCCCCCCGCGGGGGCGTGGATCGAAACT.TGAAGACCAGAT..CCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                   <  1:1518778/101‑1 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcac                                                   <  1:1265737/101‑3 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                                  <  1:6216562/101‑2 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                                 >  1:5673175/1‑97 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                <  1:1805483/101‑4 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                <  1:877093/101‑4 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                >  2:2443219/1‑98 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                >  2:4768348/1‑98 (MQ=2)
                                                         cTCCCCCCTCTGGGCCGTAGATCGAAACC.GGTATGGATCTG..GCGCAAACGG..AAAGGCT.G.CCGTCGCTCCCCCCGCGGGGTCCTGGATCGCAACCTagggcg                                             >  2:662478/1‑101 (MQ=25)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                >  2:5639260/1‑96 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcacccg                                                >  1:4047009/1‑96 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatga                                               >  1:591717/1‑97 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggcg                                             >  2:1272343/1‑101 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                               >  2:2660423/1‑95 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                              >  2:6854855/1‑94 (MQ=0)
                                                           ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                              >  1:6378595/1‑94 (MQ=0)
                                                           ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                           >  2:3771917/1‑101 (MQ=255)
                                                             ccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgc                                            <  1:732974/101‑10 (MQ=2)
                                                              cccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                           <  1:2784332/101‑11 (MQ=2)
                                                                cgcgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTg                                      >  1:2018385/1‑101 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                                    >  1:7034299/1‑101 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                                    >  1:1126104/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                                  <  2:1718481/101‑1 (MQ=255)
                                                                        gggggATCGAAAGT.GGTATGGCTCTG..GCGCAAACGG..AAAGTCT.G.TCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                              <  2:3304804/98‑1 (MQ=38)
                                                                        cGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                              >  2:590718/1‑101 (MQ=255)
                                                                        cGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGCGaa                              >  1:3445344/1‑100 (MQ=255)
                                                                         gTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                             >  2:6006092/1‑101 (MQ=255)
                                                                          tGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                            <  1:3158795/101‑1 (MQ=255)
                                                                          tGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                            <  2:4400942/101‑1 (MQ=255)
                                                                           ggATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGa                           >  2:4353734/1‑101 (MQ=255)
                                                                            gATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                          <  1:6922313/101‑1 (MQ=255)
                                                                            gATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                          <  1:2974921/101‑1 (MQ=255)
                                                                             aTCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                         >  2:3559720/1‑101 (MQ=255)
                                                                             aTCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg                         >  2:6926651/1‑101 (MQ=255)
                                                                              tCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                        >  2:849414/1‑101 (MQ=255)
                                                                              tCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                        <  1:162333/101‑1 (MQ=255)
                                                                              tCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc                        >  1:4400616/1‑101 (MQ=255)
                                                                               cGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc                       >  2:6704799/1‑101 (MQ=255)
                                                                                 aaaCT.GGTATGGCTCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGCGGCGTGAGTTGCCAGTGATAGACGCCgg                     >  1:6337471/1‑100 (MQ=255)
                                                                                    cT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa                  >  2:3924652/1‑101 (MQ=255)
                                                                                    cT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa                  <  2:2554479/101‑1 (MQ=255)
                                                                                       ggTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg                >  1:4970405/1‑101 (MQ=255)
                                                                                       ggTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGGggcag                >  2:1652860/1‑98 (MQ=255)
                                                                                        gTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGt               >  1:3370890/1‑101 (MQ=255)
                                                                                             gATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTc          >  1:6820530/1‑101 (MQ=255)
                                                                                              aTCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcc         >  1:145698/1‑101 (MQ=255)
                                                                                               tCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTccc        <  2:5133283/101‑1 (MQ=255)
                                                                                                cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccc       <  1:3580072/101‑1 (MQ=255)
                                                                                                  g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc     <  1:470451/101‑1 (MQ=255)
                                                                                                  g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc     <  1:5573648/101‑1 (MQ=255)
                                                                                                  g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTcccccc     <  2:105770/101‑1 (MQ=255)
                                                                                                     gcgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcg    <  1:6102956/101‑1 (MQ=255)
                                                                                                      cgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcgc   <  1:1004213/101‑1 (MQ=255)
                                                                                                       gcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCcgcg  >  1:534476/1‑101 (MQ=255)
                                                                                                       |                                                                                                        
CGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCG  >  NC_014034/1395646‑1395846

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG