Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,763 C→G 16.0% intergenic (+575/‑356) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7630C→G16.0% 167.5 ‑0.6 ‑0.0453intergenic (+575/‑356)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (33/40):  ref base (189/190):  total (223/230)
Fisher's exact test strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 511 total aligned reads displayed.

ACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1395663‑1395863
                                                                                                           |                                                                                                       
aCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tc                                                                                                         <  2:2916430/101‑1 (MQ=255)
  aTCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                                                       <  2:5259916/101‑1 (MQ=255)
   tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcgtc                                                                                                      >  1:1394435/1‑101 (MQ=255)
    cTGCAGGTAGGCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.tcgtcg                                                                                                     <  2:3415184/101‑1 (MQ=255)
      gCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                                                   >  1:6718741/1‑101 (MQ=255)
           tAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccccc                                                                                              >  1:3595339/1‑101 (MQ=255)
            aggcggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccccc                                                                                             <  2:2801481/98‑1 (MQ=255)
               cggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgcg                                                                                          >  2:5002472/1‑101 (MQ=255)
                 gcggGCAGTGCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggg                                                                                        <  2:6271934/101‑1 (MQ=255)
                  cggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgggg                                                                                       <  1:3424807/101‑1 (MQ=255)
                   gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggggg                                                                                      >  2:3620416/1‑101 (MQ=255)
                   gggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggggg                                                                                      >  1:3264346/1‑101 (MQ=255)
                    ggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGc                                                                                     <  1:995056/101‑1 (MQ=255)
                      cAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCgg                                                                                   >  2:566021/1‑100 (MQ=255)
                       aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                                                  >  1:6601325/1‑101 (MQ=255)
                         ttCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                                <  1:2118871/101‑1 (MQ=255)
                           ggAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              <  1:1739635/100‑1 (MQ=255)
                           caagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTgg                                                                                 <  2:5916752/94‑1 (MQ=2)
                            gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  2:137465/101‑1 (MQ=255)
                              gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  1:4801360/95‑1 (MQ=0)
                              gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  1:4093980/95‑1 (MQ=0)
                              gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  1:275251/95‑1 (MQ=0)
                              gattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              <  2:7033313/97‑1 (MQ=2)
                              gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           <  1:5240914/101‑1 (MQ=255)
                               attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  1:623513/98‑1 (MQ=2)
                                ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                            <  2:3126426/99‑1 (MQ=2)
                                gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           >  1:3948060/5‑101 (MQ=2)
                                cGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                         <  2:2559698/101‑1 (MQ=255)
                                 tcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           <  2:80669/100‑1 (MQ=2)
                                 tcAGTCGCTCCCACCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           >  2:6607103/2‑101 (MQ=2)
                                 gCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                        <  2:2060898/101‑1 (MQ=255)
                                 ctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          >  2:5847287/4‑101 (MQ=2)
                                  tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                         <  2:6093098/99‑1 (MQ=2)
                                  cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          >  1:4446939/1‑101 (MQ=2)
                                  cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          >  2:4640535/1‑101 (MQ=2)
                                  cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                                       <  1:2541077/101‑1 (MQ=255)
                                   aGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                                      >  1:5133543/1‑101 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTGGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                                                       >  1:6331264/1‑101 (MQ=2)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                                                      <  2:16025/101‑2 (MQ=2)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg                                                                    >  1:1872538/1‑101 (MQ=255)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                                                      >  2:2375691/1‑98 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                                     <  2:6912027/101‑3 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                                    >  2:110316/1‑98 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                                    <  1:877093/101‑4 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                                    >  2:4768348/1‑98 (MQ=2)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                                  >  1:1265393/1‑96 (MQ=2)
                                          ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                                  >  1:6378595/1‑94 (MQ=0)
                                          ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                                  >  1:1145656/1‑94 (MQ=0)
                                          ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTg                                                              >  2:3771917/1‑101 (MQ=255)
                                            ccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgc                                                                <  1:732974/101‑10 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACT.GGTATGGAGCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAg                                                            >  1:3707032/1‑101 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGtt                                                          <  1:4289269/101‑1 (MQ=255)
                                              ccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGtt                                                          <  1:1502744/101‑1 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGc                                                        <  1:1296225/101‑1 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGc                                                        <  2:4040931/101‑1 (MQ=255)
                                                 cgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGcc                                                       >  1:7034299/1‑101 (MQ=255)
                                                  gggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCa                                                      >  2:4377185/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAg                                                     >  1:2391559/1‑101 (MQ=255)
                                                   ggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAg                                                     <  2:1673277/101‑1 (MQ=255)
                                                    gggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACCTGCG.GCGTAAGTTGCCacc                                                    >  2:1955142/1‑99 (MQ=255)
                                                       gggggATCGAAAGT.GGTATGGCTCTG..GCGCAAACGG..AAAGTCT.G.TCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGAt                                                 <  2:3304804/98‑1 (MQ=38)
                                                       cGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGAt                                                 >  1:1983506/1‑101 (MQ=255)
                                                       cGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGAt                                                 <  1:4661102/101‑1 (MQ=255)
                                                        gTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                >  2:6006092/1‑101 (MQ=255)
                                                        gTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                <  2:1237886/101‑1 (MQ=255)
                                                           gATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.c                                            <  1:2974921/101‑1 (MQ=255)
                                                            aTCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.Cg                                           >  2:3559720/1‑101 (MQ=255)
                                                             tCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGc                                          >  1:3116538/1‑101 (MQ=255)
                                                                  aCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTgg                                     >  2:4169376/1‑101 (MQ=255)
                                                                   cT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                    >  2:3924652/1‑101 (MQ=255)
                                                                   cT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                    <  1:5872485/101‑1 (MQ=255)
                                                                    t.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                  >  1:2315831/1‑101 (MQ=255)
                                                                    t.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                  <  1:6992403/101‑1 (MQ=255)
                                                                      ggTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.Cg                                 >  1:2627719/1‑101 (MQ=255)
                                                                           ggATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                            <  2:4236364/101‑1 (MQ=255)
                                                                           ggATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                            >  1:5542129/1‑101 (MQ=255)
                                                                               cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                        >  2:2678826/1‑101 (MQ=255)
                                                                               cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                        >  1:5093472/1‑101 (MQ=255)
                                                                               cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                        <  1:3502798/101‑1 (MQ=255)
                                                                                 g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                      <  2:668253/101‑1 (MQ=255)
                                                                                 g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                      <  1:6838086/101‑1 (MQ=255)
                                                                                    gcgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcg                     <  1:6102956/101‑1 (MQ=255)
                                                                                     cgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgc                    <  1:1004213/101‑1 (MQ=255)
                                                                                      gcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgcg                   >  1:534476/1‑101 (MQ=255)
                                                                                        aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                 >  2:4520281/1‑101 (MQ=255)
                                                                                        aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                 <  1:4853418/101‑1 (MQ=255)
                                                                                        aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                 >  2:6825699/1‑101 (MQ=255)
                                                                                         aaCGG..AAAGTCT.G.TCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgggg                <  2:1872466/101‑1 (MQ=255)
                                                                                          aCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggggg               >  2:2435077/1‑101 (MQ=255)
                                                                                           cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc              >  1:1541508/1‑101 (MQ=255)
                                                                                            gg..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCg             >  1:4584742/1‑101 (MQ=255)
                                                                                             g..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCgg            >  2:5520865/1‑100 (MQ=255)
                                                                                                  ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt            >  1:3855375/10‑101 (MQ=0)
                                                                                                   gTCT.C.TCGGCGCGCCCGACGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCACCCCCCGCGGGGGCGTGGAt        <  2:2163924/101‑1 (MQ=38)
                                                                                                    tCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc       >  2:6452920/1‑101 (MQ=255)
                                                                                                    tCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc       >  2:1800232/1‑101 (MQ=255)
                                                                                                        g.tcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    >  1:5769435/1‑101 (MQ=255)
                                                                                                        g.tcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    >  2:6578231/1‑101 (MQ=255)
                                                                                                          tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   <  2:5138307/101‑1 (MQ=255)
                                                                                                           cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  1:6019221/101‑1 (MQ=255)
                                                                                                           |                                                                                                       
ACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1395663‑1395863

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG