Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,828 G→C 2.7% intergenic (+640/‑291) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8280G→C 2.7% 11.2 ‑3.3 ‑0.2330intergenic (+640/‑291)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/1):  ref base (165/156):  total (173/157)
Fisher's exact test strand distribution p-value = 3.85e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.24e-01

Only 100 of 345 total aligned reads displayed.

ACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395729‑1395928
                                                                                                     |                                                                                                     
  tGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                                                                                   <  2:3928366/101‑1 (MQ=255)
  tGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                                                                                   <  1:6992403/101‑1 (MQ=255)
   ggTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTTATAGA.CGCCGTGGA.Cg                                                                                                  >  1:3443011/1‑101 (MQ=255)
   ggTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.Cg                                                                                                  <  2:2140568/101‑1 (MQ=255)
   ggTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.Cg                                                                                                  >  1:4970405/1‑101 (MQ=255)
   ggTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.Cg                                                                                                  >  1:1621421/1‑101 (MQ=255)
        ggATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                                                                                             >  1:5542129/1‑101 (MQ=255)
           gatGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccc                                                                                          >  2:1096564/3‑101 (MQ=255)
            cTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                                                         >  1:5093472/1‑101 (MQ=255)
            cTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                                                                                         <  1:3502798/101‑1 (MQ=255)
             tGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccccc                                                                                        <  1:279064/101‑1 (MQ=255)
              ggCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                                                                                       <  1:6838086/101‑1 (MQ=255)
              ggCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                                                                                       >  2:3414095/1‑101 (MQ=255)
              ggCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                                                                                       >  2:2502166/1‑101 (MQ=255)
                cgcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgc                                                                                     <  1:1004213/101‑1 (MQ=255)
                 gcAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgcg                                                                                    >  1:534476/1‑101 (MQ=255)
                   aaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                                                                                  >  2:4520281/1‑101 (MQ=255)
                   aaaCGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                                                                                  <  1:4853418/101‑1 (MQ=255)
                      cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                                               <  2:2791213/101‑1 (MQ=255)
                      cGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                                                               >  2:4658427/1‑101 (MQ=255)
                       ggAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCg                                                                              >  1:3676897/1‑101 (MQ=255)
                        cggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCg                                                                              >  2:2715775/11‑101 (MQ=0)
                        cggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCg                                                                              >  2:3077558/11‑101 (MQ=0)
                         aaaGACTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                            <  1:3326216/101‑1 (MQ=255)
                          aaGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                           >  2:4266537/1‑101 (MQ=255)
                            gTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         <  1:932287/101‑1 (MQ=255)
                            gTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGCGGA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                         >  2:4194370/1‑101 (MQ=255)
                               tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                      >  1:4385331/1‑101 (MQ=255)
                               tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                      <  1:6261863/101‑1 (MQ=255)
                                gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  2:1929666/101‑1 (MQ=255)
                                gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     >  2:1287157/1‑101 (MQ=255)
                                gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     >  2:6578231/1‑101 (MQ=255)
                                gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  2:3595203/101‑1 (MQ=255)
                                gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     >  1:5769435/1‑101 (MQ=255)
                                gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                     <  1:2596658/101‑1 (MQ=255)
                                 tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                    <  2:5138307/101‑1 (MQ=255)
                                  cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   >  1:2067203/1‑101 (MQ=255)
                                  cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   >  1:1103502/1‑101 (MQ=255)
                                  cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                   <  1:6019221/101‑1 (MQ=255)
                                   gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGC.CGGCGCTCCCCCCGCGGGGCCGGGGATCCACACt                                                                  >  1:2286003/1‑101 (MQ=37)
                                   gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                  >  1:3049530/1‑101 (MQ=255)
                                     cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                                >  1:1272395/1‑101 (MQ=255)
                                     cgCCCCCCCCGCGGGGGCGTGGATCGAAACCCGCC.GCGTGAGTTGCCAGTGATAGA.CGGCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                                <  2:2286733/101‑1 (MQ=37)
                                           ccccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCACCCGCGGGGGGGTGGATCGAAACTGCgccgcc                                                          >  1:5173274/1‑101 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                        <  1:590738/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTa                                                        <  1:1922053/101‑1 (MQ=255)
                                              cgcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                       <  2:73984/101‑1 (MQ=255)
                                              cgcgGGGGCGGGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                       <  2:6369910/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACGTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                  <  2:4559426/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                  >  2:2985791/1‑101 (MQ=255)
                                                   gggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGACGCCTATCGCAt                                                  <  1:4542491/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                 <  2:534464/101‑1 (MQ=255)
                                                     gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                <  1:4055940/101‑1 (MQ=255)
                                                      cGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                               >  1:4431916/1‑101 (MQ=255)
                                                      cGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                               <  2:5069139/101‑1 (MQ=255)
                                                       gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                              >  2:5933265/1‑101 (MQ=255)
                                                       gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                              >  2:5997785/1‑101 (MQ=255)
                                                        tGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGt                                             <  1:1791002/101‑1 (MQ=255)
                                                        tGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGt                                             >  2:1655468/1‑101 (MQ=255)
                                                        gggATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGt                                             >  2:6047636/2‑101 (MQ=255)
                                                         ggATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTc                                            <  2:1537139/101‑1 (MQ=255)
                                                         ggATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTc                                            <  2:1126056/101‑1 (MQ=255)
                                                          gATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCg                                           <  2:3370767/101‑1 (MQ=255)
                                                           aTCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGa                                          >  1:666447/1‑101 (MQ=255)
                                                             cGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgc                                        >  1:1421257/1‑101 (MQ=255)
                                                               aaaCCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgcgc                                      <  2:1425726/101‑1 (MQ=255)
                                                               aaaCCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGGCGAgcac                                      >  2:965544/1‑99 (MQ=255)
                                                                  ccTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGcgccgc                                   <  2:5394707/101‑1 (MQ=255)
                                                                   cTGCG.GCGTGAGTTGCCAGTCATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCcgcg                                  <  1:5624248/101‑1 (MQ=255)
                                                                    tGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCgcgc                                 >  2:1008266/1‑101 (MQ=255)
                                                                       g.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGt                              >  2:5723030/1‑101 (MQ=255)
                                                                       g.gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGt                              <  2:4048292/101‑1 (MQ=255)
                                                                         gcgTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTc                             >  2:3808890/1‑101 (MQ=255)
                                                                           gTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGc                           >  2:7073200/1‑101 (MQ=255)
                                                                           gTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGc                           >  1:1968097/1‑101 (MQ=255)
                                                                            tGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCt                          <  2:1894351/101‑1 (MQ=255)
                                                                              aGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcc                        <  2:6626703/101‑1 (MQ=255)
                                                                              aGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcc                        >  2:2604770/1‑101 (MQ=255)
                                                                                ttGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccc                      <  1:3591969/101‑1 (MQ=255)
                                                                                 tGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTccccc                     >  1:1153256/1‑101 (MQ=255)
                                                                                  gCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccccc                    <  2:2391465/101‑1 (MQ=255)
                                                                                  gCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccccc                    >  2:4153204/1‑101 (MQ=255)
                                                                                   ccAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcg                   <  1:3814721/101‑1 (MQ=255)
                                                                                   ccAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcg                   <  2:557807/101‑1 (MQ=255)
                                                                                    cAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTcccccccc                  >  1:391893/1‑99 (MQ=255)
                                                                                    cAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcgc                  <  1:5121549/101‑1 (MQ=255)
                                                                                    cAGTGATAGA.CGCCGTGGA.CGTCGCTCCACCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCcgc                  >  1:5070080/1‑101 (MQ=255)
                                                                                      gTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCgg                <  1:4678799/101‑1 (MQ=255)
                                                                                      gTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCgg                <  2:5313179/101‑1 (MQ=255)
                                                                                         aTAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCggggg             <  2:6824660/101‑1 (MQ=255)
                                                                                             a.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTg         <  2:6837355/101‑1 (MQ=255)
                                                                                                gCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGa       >  1:2419733/1‑101 (MQ=255)
                                                                                                gCCGTGGA.CGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGa       >  2:1707508/1‑101 (MQ=255)
                                                                                                 ccGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt      <  2:777134/101‑1 (MQ=255)
                                                                                                   gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  1:1492773/1‑101 (MQ=255)
                                                                                                   gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  2:4813314/1‑101 (MQ=255)
                                                                                                   gTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGAGCg    >  1:6725861/1‑101 (MQ=255)
                                                                                                    tGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  1:2679016/101‑1 (MQ=255)
                                                                                                     ggA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  >  1:4734872/1‑101 (MQ=255)
                                                                                                     ggA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  2:3868151/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
ACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395729‑1395928

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG