Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,864 T→C 7.9% intergenic (+676/‑255) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8640T→C 7.9% 72.3 ‑1.2 ‑0.8452intergenic (+676/‑255)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/22):  ref base (203/211):  total (217/235)
Fisher's exact test strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.43e-01

Only 100 of 480 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTG  >  NC_014034/1395764‑1395964
                                                                                                    |                                                                                                              
gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                                <  1:5032050/101‑1 (MQ=255)
gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                                <  2:4476674/101‑1 (MQ=255)
     tCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.cc                                                                                                          >  1:5096791/1‑101 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                                                     <  2:1103467/101‑1 (MQ=255)
            gcgGGGGCGTGGATTGAAAGCCGCGGGGACGGTGGGCAGTGAAAGCGGCGGTGGACGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.CCGC.CTATc                                                                                                  <  2:1917150/101‑1 (MQ=16)
            gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATc                                                                                                  <  1:6775150/101‑1 (MQ=255)
                gggCGTGGATCGAAACGTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                              <  2:4559426/101‑1 (MQ=255)
                gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                              <  2:714684/101‑1 (MQ=255)
                 ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                             <  2:534464/101‑1 (MQ=255)
                  gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                                            <  1:3924827/101‑1 (MQ=255)
                   cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                           <  2:5069139/101‑1 (MQ=255)
                    gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcccg                                                                                          >  2:1002927/1‑99 (MQ=255)
                     tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                                                      >  2:3591833/1‑101 (MQ=255)
                      ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                     <  2:1537139/101‑1 (MQ=255)
                       gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCg                                                                                    <  2:3370767/101‑1 (MQ=255)
                        aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGa                                                                                   >  1:666447/1‑101 (MQ=255)
                         tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                  <  1:1461541/101‑1 (MQ=255)
                            aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                               >  1:2365769/1‑101 (MQ=255)
                            aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                               <  2:1425726/101‑1 (MQ=255)
                            aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                               >  2:6606208/1‑101 (MQ=255)
                            aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgagc                                                                               >  2:939758/1‑101 (MQ=255)
                               ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgc.c.gc                                                                          <  2:5394707/101‑1 (MQ=255)
                                         gAGTTGCCAGTTATAGACGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTc                                                             <  2:5688779/101‑1 (MQ=255)
                                             tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTccccc                                                         >  1:1153256/1‑101 (MQ=255)
                                              gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTcccccc                                                        <  2:2963274/101‑1 (MQ=255)
                                               ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcg                                                       <  2:557807/101‑1 (MQ=255)
                                               ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcg                                                       <  2:2349701/101‑1 (MQ=255)
                                                     aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCggggg                                                 >  2:5188918/1‑101 (MQ=255)
                                                         aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTg                                             <  1:3772078/101‑1 (MQ=255)
                                                          gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGCCCTC.GCG.C...GTCGCTCCCCCCGCGGGGGCGTgg                                            <  2:6316965/91‑1 (MQ=9)
                                                          gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTgg                                            <  1:3254366/91‑1 (MQ=9)
                                                          gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTgg                                            >  2:2665653/11‑101 (MQ=9)
                                                          ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCg                                               >  1:4052645/10‑101 (MQ=2)
                                                          cGCGGTGGACGTCCCTCCCCCCGTGGGGGCGGGGATCGAACCTGCG.CCAC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTgg                                            <  2:4070035/101‑1 (MQ=38)
                                                           gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                           >  1:1726573/1‑101 (MQ=255)
                                                           gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                           >  2:4661403/1‑101 (MQ=255)
                                                           gCCGTGGACGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                           >  2:1707508/1‑101 (MQ=255)
                                                           cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGt                                              >  1:1967288/9‑101 (MQ=2)
                                                           ccatcctaggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGa                                           <  1:3586215/92‑1 (MQ=9)
                                                            ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  1:4839583/101‑1 (MQ=255)
                                                            caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGAt                                          >  1:5442037/9‑101 (MQ=9)
                                                            caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGAt                                          >  1:3109116/9‑101 (MQ=9)
                                                             gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTgg                                            <  1:3399308/95‑1 (MQ=2)
                                                             cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  1:6020280/101‑1 (MQ=255)
                                                             aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  1:4456625/8‑101 (MQ=9)
                                                             aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGGGGATc                                         >  1:5350814/8‑101 (MQ=9)
                                                              gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                        >  2:4813314/1‑101 (MQ=255)
                                                               ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                          >  2:5117400/5‑101 (MQ=2)
                                                               ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  2:6999633/97‑1 (MQ=2)
                                                                ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                      >  1:4734872/1‑101 (MQ=255)
                                                                ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                      <  2:3868151/101‑1 (MQ=255)
                                                                cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  2:1357672/98‑1 (MQ=2)
                                                                ccccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGC.CATCGAG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                        <  1:6492509/98‑1 (MQ=2)
                                                                 tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCGCC.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                     >  1:1899076/4‑101 (MQ=2)
                                                                 ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                        <  1:96252/99‑1 (MQ=2)
                                                                 ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                        >  1:1100936/3‑101 (MQ=2)
                                                                  atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                    <  1:5839722/99‑1 (MQ=9)
                                                                  atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                    <  1:6392523/99‑1 (MQ=9)
                                                                   tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                   <  2:1266167/100‑2 (MQ=9)
                                                                   tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                   >  2:5975960/2‑100 (MQ=9)
                                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                   >  2:3250990/1‑101 (MQ=255)
                                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                   <  1:1287205/101‑1 (MQ=255)
                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGC.CATCGAC.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                    <  1:3702897/101‑1 (MQ=2)
                                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                  >  2:438729/1‑101 (MQ=255)
                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                 >  2:2445926/1‑101 (MQ=255)
                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                >  1:29932/1‑101 (MQ=255)
                                                                      cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                <  2:1659801/101‑1 (MQ=255)
                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                               <  1:4658666/101‑1 (MQ=255)
                                                                        ctcgcgccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.CTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                              <  2:5855268/95‑1 (MQ=2)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAACCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                              <  2:2026815/101‑1 (MQ=255)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCCCGGGGGCGCGGATCGAAACGAGGCg                              >  1:1793627/1‑101 (MQ=255)
                                                                        cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                              >  1:3513174/1‑101 (MQ=9)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                             >  2:2631060/1‑101 (MQ=255)
                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                            >  1:6938221/1‑101 (MQ=255)
                                                                          caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                            <  2:4147385/99‑3 (MQ=9)
                                                                          caccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                            >  1:596005/3‑99 (MQ=9)
                                                                           accccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                           >  2:1042178/2‑98 (MQ=9)
                                                                            ccccGGGGGGGCGGGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                          <  1:4169571/101‑1 (MQ=255)
                                                                            ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                          <  2:6411699/101‑1 (MQ=255)
                                                                              ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                        >  1:1623534/1‑101 (MQ=255)
                                                                              ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                           <  2:6021689/101‑10 (MQ=2)
                                                                               cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTTGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                       <  2:3442887/101‑1 (MQ=255)
                                                                               cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                       <  2:3635104/101‑1 (MQ=255)
                                                                                 cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                     >  1:1394390/1‑92 (MQ=2)
                                                                                 cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                     >  1:3851113/1‑101 (MQ=255)
                                                                                  gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                    <  2:6124289/101‑11 (MQ=2)
                                                                                  gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                    <  2:6875624/101‑11 (MQ=2)
                                                                                  gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                    >  1:558831/1‑101 (MQ=255)
                                                                                    gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                  <  1:288914/101‑1 (MQ=255)
                                                                                    gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                  <  1:2713429/101‑1 (MQ=255)
                                                                                    gggCGCTGATCGAACCTGCC.CCGC.CTGCCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCttc                  <  2:6725519/101‑3 (MQ=25)
                                                                                      gCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                <  1:1002962/101‑1 (MQ=255)
                                                                                       cGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc               >  2:4003795/1‑101 (MQ=255)
                                                                                       cGTGGATCGAAACGGCG.CCGC.CTAGCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc               <  2:2234096/101‑1 (MQ=255)
                                                                                         tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg             >  1:2071116/1‑101 (MQ=255)
                                                                                                aaaCTGCT.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg      <  2:5621019/101‑1 (MQ=255)
                                                                                                aaaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGGAGGgg      >  1:250248/1‑101 (MQ=255)
                                                                                                aaaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg      >  1:4298060/1‑101 (MQ=255)
                                                                                                aaaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg      <  1:5723332/101‑1 (MQ=255)
                                                                                                   cTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGCCt   >  2:788534/1‑101 (MQ=255)
                                                                                                    |                                                                                                              
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTG  >  NC_014034/1395764‑1395964

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG