Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,866 C→T 19.2% intergenic (+678/‑253) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8660C→T19.2% 235.9 ‑1.5 0.0443intergenic (+678/‑253)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (36/49):  ref base (178/180):  total (214/229)
Fisher's exact test strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGT  >  NC_014034/1395766‑1395966
                                                                                                    |                                                                                                              
cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgc                                                                                                                >  2:1379938/1‑101 (MQ=255)
      ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCACCCGCGGGGGGGTGGATCGAAACTGCg.ccgc.c                                                                                                        >  1:5173274/1‑101 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTa                                                                                                      <  1:590738/101‑1 (MQ=255)
             ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCa                                                                                                 >  1:4007822/1‑101 (MQ=255)
              gggCGTTGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCTCTCCCCCCGCGGGGGCGTGGATCGAAGCTGCG.CCGC.CTATCGCAt                                                                                                <  1:6723444/101‑1 (MQ=255)
              gggCGTGGATCGAAACGTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                <  2:4559426/101‑1 (MQ=255)
              gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                <  2:714684/101‑1 (MQ=255)
              gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.ACGC.CTATCGCAt                                                                                                <  1:4542491/101‑1 (MQ=255)
               ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                               <  2:534464/101‑1 (MQ=255)
                 cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                             >  1:4431916/1‑101 (MQ=255)
                 cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                             >  1:275691/1‑101 (MQ=255)
                   tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                                                        >  1:3878181/1‑101 (MQ=255)
                   tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                                                        >  2:3591833/1‑101 (MQ=255)
                    ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                       <  1:7086572/101‑1 (MQ=255)
                     gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCg                                                                                      <  2:3370767/101‑1 (MQ=255)
                      aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGa                                                                                     <  1:4091486/101‑1 (MQ=255)
                      aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGa                                                                                     <  1:6006397/101‑1 (MQ=255)
                       tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                    <  1:1461541/101‑1 (MQ=255)
                       tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                    >  1:351732/1‑101 (MQ=255)
                         gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcg                                                                                  >  2:4209174/1‑101 (MQ=255)
                         gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcg                                                                                  >  2:5046733/1‑101 (MQ=255)
                         gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcg                                                                                  >  2:1450736/1‑101 (MQ=255)
                          aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                                 >  2:6606208/1‑101 (MQ=255)
                          aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgagc                                                                                 >  2:939758/1‑101 (MQ=255)
                             ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgc.c.gc                                                                            <  2:5394707/101‑1 (MQ=255)
                             ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgc.c.gc                                                                            >  2:1920034/1‑101 (MQ=255)
                                  ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.Gt                                                                    <  2:4048292/101‑1 (MQ=255)
                                       gAGTTGCCAGTTATAGACGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTc                                                               <  2:5688779/101‑1 (MQ=255)
                                        aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTcc                                                              <  2:1272347/101‑1 (MQ=255)
                                           tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTccccc                                                           >  1:1153256/1‑101 (MQ=255)
                                             ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcg                                                         <  1:3736609/101‑1 (MQ=255)
                                             ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcg                                                         <  2:557807/101‑1 (MQ=255)
                                             ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcg                                                         <  2:2349701/101‑1 (MQ=255)
                                                  gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCgggg                                                    >  2:2864232/1‑101 (MQ=255)
                                                   aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCggggg                                                   >  2:5188918/1‑101 (MQ=255)
                                                    tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCTGGGGc                                                  <  1:5637625/101‑1 (MQ=255)
                                                    tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGc                                                  >  2:2481263/1‑101 (MQ=255)
                                                     aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCg                                                 >  2:6834798/1‑101 (MQ=255)
                                                      gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGt                                                >  2:1004433/1‑101 (MQ=255)
                                                        gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGCCCTC.GCG.C...GTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:6316965/91‑1 (MQ=9)
                                                        gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTgg                                              >  2:2665653/11‑101 (MQ=9)
                                                         gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                             >  1:1726573/1‑101 (MQ=255)
                                                         gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                             >  2:4661403/1‑101 (MQ=255)
                                                         gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                             >  1:2419733/1‑101 (MQ=255)
                                                         gCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAGACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:5085521/101‑1 (MQ=255)
                                                         cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGt                                                >  1:1967288/9‑101 (MQ=2)
                                                           gcccggcGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:1118135/95‑1 (MQ=2)
                                                           aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATc                                           >  2:1563305/8‑101 (MQ=9)
                                                            gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  2:4813314/1‑101 (MQ=255)
                                                            gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.CCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  1:3635253/1‑101 (MQ=255)
                                                             cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGCCCTC.GCG.C...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  2:3621060/96‑1 (MQ=9)
                                                             ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  2:6999633/97‑1 (MQ=2)
                                                              ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:3014685/97‑1 (MQ=9)
                                                              cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:4892822/98‑1 (MQ=2)
                                                               tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCGCC.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  1:1899076/4‑101 (MQ=2)
                                                               tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:5618808/4‑101 (MQ=9)
                                                               tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:3828038/4‑101 (MQ=9)
                                                               tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCACCGCGGGGGCGTGGATCGaaa                                       >  2:3183540/4‑101 (MQ=9)
                                                               ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  1:1100936/3‑101 (MQ=2)
                                                               ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  2:5117031/3‑101 (MQ=2)
                                                               cccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGC.CATCGAG.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         >  2:6074197/3‑101 (MQ=2)
                                                                 tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                     >  1:4688879/2‑100 (MQ=9)
                                                                 tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                     >  2:5975960/2‑100 (MQ=9)
                                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                     <  1:1287205/101‑1 (MQ=255)
                                                                  gTCGCTCCCCTCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  2:4146182/101‑1 (MQ=2)
                                                                    cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                  <  2:1659801/101‑1 (MQ=255)
                                                                     gCTCCCCCCGGGGGGGCGGGGATCGAAACGGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                 <  2:1792732/101‑1 (MQ=255)
                                                                      ctcgcgccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.CTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                <  2:5855268/95‑1 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAACCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                <  2:2026815/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACTGNG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                <  1:3192165/101‑1 (MQ=255)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCCCGGGGGCGCGGATCGAAACGAGGCg                                >  1:1793627/1‑101 (MQ=255)
                                                                       tcaccccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                               <  2:1050617/98‑2 (MQ=9)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                               <  1:2533538/101‑2 (MQ=9)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                               >  1:4816346/1‑101 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                               >  2:2631060/1‑101 (MQ=255)
                                                                         cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                             >  1:3796049/1‑101 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                            <  1:3267562/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                            <  2:6411699/101‑1 (MQ=255)
                                                                          ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                            <  1:4209369/101‑1 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                          >  1:1623534/1‑101 (MQ=255)
                                                                            ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                             <  2:6021689/101‑10 (MQ=2)
                                                                             cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTTGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                         <  2:3442887/101‑1 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGc                        >  1:978833/1‑101 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                       >  1:1394390/1‑92 (MQ=2)
                                                                               cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                       <  1:6222779/101‑10 (MQ=2)
                                                                                gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                      >  1:5411603/1‑101 (MQ=255)
                                                                                 ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                     >  1:5566782/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                    <  1:6521361/101‑1 (MQ=255)
                                                                                  gggCGCTGATCGAACCTGCC.CCGC.CTGCCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCttc                    <  2:6725519/101‑3 (MQ=25)
                                                                                   ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                   >  2:4402061/1‑101 (MQ=255)
                                                                                   ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                   <  1:344881/101‑1 (MQ=255)
                                                                                    gCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                  <  1:1002962/101‑1 (MQ=255)
                                                                                     cGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCGTTCATGGCCTACGTc                 >  1:6844405/1‑101 (MQ=255)
                                                                                     cGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                 >  2:4003795/1‑101 (MQ=255)
                                                                                     cGTGGATCGAAACGGCG.CCGC.CTAGCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                 <  2:2234096/101‑1 (MQ=255)
                                                                                       tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg               >  1:6465995/1‑101 (MQ=255)
                                                                                       tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg               >  1:5784231/1‑101 (MQ=255)
                                                                                       tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg               >  1:2071116/1‑101 (MQ=255)
                                                                                                 cTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGCCt     >  2:788534/1‑101 (MQ=255)
                                                                                                    cg.ccgc.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt  <  1:298472/101‑1 (MQ=255)
                                                                                                    |                                                                                                              
CGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGT  >  NC_014034/1395766‑1395966

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG