BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,868	C→G	1.3%	intergenic (+680/‑251)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,868	0	C→G	1.3%	3.1 0.0 ‑0.0	444	intergenic (+680/‑251)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (3/3): ref base (211/226): total (214/230)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.82e-01

Only 100 of 478 total aligned reads displayed.


CTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTC  >  NC_014034/1395768‑1395967


 tCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcg.cc                                                                                                             <  1:6705141/101‑1 (MQ=255)


     cccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.Ct                                                                                                        <  2:1103467/101‑1 (MQ=255)


       cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                      <  1:2421494/101‑1 (MQ=255)


       cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTAt                                                                                                      <  1:4760016/101‑1 (MQ=255)


           ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCa                                                                                                  >  1:4007822/1‑101 (MQ=255)


           ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCa                                                                                                  >  1:1821690/1‑101 (MQ=255)


            gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCAt                                                                                                 <  2:714684/101‑1 (MQ=255)


             ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATc                                                                                                <  2:534464/101‑1 (MQ=255)


              gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                                               <  1:4055940/101‑1 (MQ=255)


              gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcc                                                                                               <  1:4884305/101‑1 (MQ=255)


               cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCg                                                                                              <  2:5069139/101‑1 (MQ=255)


                gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATcccg                                                                                             >  2:1002927/1‑99 (MQ=255)


                gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCgg                                                                                             >  1:1375471/1‑101 (MQ=255)


                 tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...t                                                                                         >  1:3878181/1‑101 (MQ=255)


                  ggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...Tc                                                                                        <  2:2766850/101‑1 (MQ=255)


                     tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAg                                                                                     >  1:351732/1‑101 (MQ=255)


                      cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgc                                                                                    <  2:2018305/101‑1 (MQ=255)


                       gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcg                                                                                   >  2:1450736/1‑101 (MQ=255)


                        aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgcgc                                                                                  >  2:6606208/1‑101 (MQ=255)


                        aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAgagc                                                                                  >  2:939758/1‑101 (MQ=255)


                           ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGcgc.c.gc                                                                             <  2:5394707/101‑1 (MQ=255)


                            cTGCGGCGTGAGTTGCCAGTCATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.c.gcg                                                                            <  1:5624248/101‑1 (MQ=255)


                                ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.Gt                                                                     <  2:3049417/101‑1 (MQ=255)


                                ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.Gt                                                                     >  2:5723030/1‑101 (MQ=255)


                                 gcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTc                                                                    >  2:3808890/1‑101 (MQ=255)


                                   gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGc                                                                  >  1:1968097/1‑101 (MQ=255)


                                           ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcg                                                          <  2:557807/101‑1 (MQ=255)


                                            cAGTGATAGACGCCGTGGACGTCGCTCCACCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCcgc                                                         >  1:5070080/1‑101 (MQ=255)


                                              gTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCgg                                                       <  1:4678799/101‑1 (MQ=255)


                                                gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCgggg                                                     >  2:265428/1‑101 (MQ=255)


                                                    gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGt                                                 <  1:2985900/101‑1 (MQ=255)


                                                     aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTg                                                <  1:4520520/101‑1 (MQ=255)


                                                     aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTg                                                <  2:6837355/101‑1 (MQ=255)


                                                      gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGCCCTC.GCG.C...GTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:6316965/91‑1 (MQ=9)


                                                      gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:3250742/91‑1 (MQ=9)


                                                      cGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:4086532/101‑1 (MQ=255)


                                                        ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  2:4169039/101‑1 (MQ=255)


                                                        caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCGCC.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:1768585/93‑1 (MQ=2)


                                                         gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:4712033/95‑1 (MQ=2)


                                                         cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATTC.A...TCGAGCGC.CATCGAG.C.GTCGCTCCCCCCGCGGGGGCGTGGa                                              >  2:4040267/7‑101 (MQ=0)


                                                         cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:351728/101‑1 (MQ=255)


                                                          gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  2:4813314/1‑101 (MQ=255)


                                                          gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.CCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:3635253/1‑101 (MQ=255)


                                                           tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  1:5753739/1‑101 (MQ=255)


                                                           ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  2:5117400/5‑101 (MQ=2)


                                                           ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  2:6999633/97‑1 (MQ=2)


                                                            ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:1930642/101‑1 (MQ=255)


                                                            cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:1357672/98‑1 (MQ=2)


                                                            cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGGGGAta                                            >  2:1703380/4‑100 (MQ=2)


                                                             ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:1100936/3‑101 (MQ=2)


                                                             ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:5879403/3‑101 (MQ=2)


                                                              gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  1:1155281/100‑1 (MQ=2)


                                                              atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       >  1:700196/3‑101 (MQ=9)


                                                              atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:3108723/99‑1 (MQ=9)


                                                              aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  2:4400394/101‑1 (MQ=255)


                                                              aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:6453250/101‑1 (MQ=255)


                                                               tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                      <  2:1266167/100‑2 (MQ=9)


                                                               tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                      >  2:5975960/2‑100 (MQ=9)


                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                     >  1:5553267/1‑101 (MQ=255)


                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                     >  1:6804491/1‑101 (MQ=255)


                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:2340287/101‑1 (MQ=2)


                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  1:2772260/101‑1 (MQ=2)


                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                    >  2:2445926/1‑101 (MQ=255)


                                                                 ccGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                    <  1:2110095/100‑1 (MQ=255)


                                                                  cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                   >  1:3744026/1‑101 (MQ=9)


                                                                  cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                   <  2:3951784/101‑1 (MQ=9)


                                                                    ctcgcgccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.CTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                 <  2:5855268/95‑1 (MQ=2)


                                                                       cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                              >  1:3796049/1‑101 (MQ=255)


                                                                       cccccGCGGGGGCGTGGATCGAAACTGCG.CCCC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                              <  1:3309835/101‑1 (MQ=255)


                                                                       accccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                              >  2:1042178/2‑98 (MQ=9)


                                                                        ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                             <  1:4209369/101‑1 (MQ=255)


                                                                        ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                             <  2:6411699/101‑1 (MQ=255)


                                                                         cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                            <  1:1480303/101‑1 (MQ=255)


                                                                          ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGACGGTCTTCTGGTGC.C.GCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                              <  2:6021689/101‑10 (MQ=2)


                                                                              gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                       <  2:6124289/101‑11 (MQ=2)


                                                                              gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                       <  2:1899004/101‑11 (MQ=2)


                                                                              gggggCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGACGC...TGGGGC.C.C.TCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAAATGCggcgcgggcct                       >  2:4569629/1‑91 (MQ=2)


                                                                              gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                       <  1:6053588/101‑1 (MQ=255)


                                                                               ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                      <  1:965578/101‑1 (MQ=255)


                                                                                gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                     <  1:2534940/101‑1 (MQ=255)


                                                                                gggCGCTGATCGAACCTGCC.CCGC.CTGCCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCttc                     <  2:6725519/101‑3 (MQ=25)


                                                                                 ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                    <  1:344881/101‑1 (MQ=255)


                                                                                 ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                    <  1:1652063/101‑1 (MQ=255)


                                                                                 ggCGGGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                    <  1:5361781/101‑1 (MQ=255)


                                                                                  gCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                   <  1:1002962/101‑1 (MQ=255)


                                                                                   cGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                  <  1:4395873/101‑1 (MQ=255)


                                                                                    gTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTAGATCGAAACGATGCGTTCATGGCCTACGTCg                 >  1:5123556/1‑101 (MQ=255)


                                                                                     tGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                >  1:650511/1‑101 (MQ=255)


                                                                                      ggATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg               >  1:1436211/1‑101 (MQ=255)


                                                                                       gATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGc              >  2:1649804/1‑101 (MQ=255)


                                                                                           gAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg          <  1:5998090/101‑1 (MQ=255)


                                                                                           gAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg          >  2:3422711/1‑101 (MQ=255)


                                                                                            aaaCTGCT.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg         <  2:5621019/101‑1 (MQ=255)


                                                                                            aaaCTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg         <  2:2189770/101‑1 (MQ=255)


                                                                                               cTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGCCt      >  2:788534/1‑101 (MQ=255)


                                                                                               cTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt      <  2:3606678/101‑1 (MQ=255)


                                                                                                tGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg     >  2:5217067/1‑101 (MQ=255)


                                                                                                  cg.ccgc.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt   <  2:2627623/101‑1 (MQ=255)


                                                                                                   g.ccgc.cTCGCCCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc  <  2:3296919/101‑1 (MQ=255)


                                                                                                   g.ccgc.cTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGCTc  <  2:1541448/101‑1 (MQ=255)


CTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATCCGG...TCGAGCGC.C.GCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTC  >  NC_014034/1395768‑1395967

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG