Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,872 C→T 19.8% intergenic (+684/‑247) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8720C→T19.8% 231.2 ‑1.5 ‑0.0455intergenic (+684/‑247)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (36/49):  ref base (167/167):  total (217/238)
Fisher's exact test strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 478 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCC  >  NC_014034/1395772‑1395972
                                                                                                    |                                                                                                         
ccccGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCACCCGCGGGGGGGTGGATCGAAACTGCgccgcc                                                                                                           >  1:5173274/1‑101 (MQ=255)
   cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                        <  2:73984/101‑1 (MQ=255)
   cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                        <  1:2421494/101‑1 (MQ=255)
   cgcgGGGGCGGGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                        <  2:6369910/101‑1 (MQ=255)
    gcgGGGGCGTGGATTGAAAGCCGCGGGGACGGTGGGCAGTGAAAGCGGCGGTGGACGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGCCGCCTATc                                                                                                       <  2:1917150/101‑1 (MQ=16)
    gcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                                                                                                       >  2:5361509/1‑101 (MQ=255)
       ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                                                                                    >  1:4007822/1‑101 (MQ=255)
       ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                                                                                    >  1:1821690/1‑101 (MQ=255)
        gggCGTTGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCTCTCCCCCCGCGGGGGCGTGGATCGAAGCTGCGCCGCCTATCGCAt                                                                                                   <  1:6723444/101‑1 (MQ=255)
          gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                 <  1:4055940/101‑1 (MQ=255)
           cGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCg                                                                                                >  2:5857493/1‑101 (MQ=255)
            gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                               >  1:1375471/1‑101 (MQ=255)
             tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...t                                                                                           >  1:3878181/1‑101 (MQ=255)
                 tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAg                                                                                       >  1:2124028/1‑101 (MQ=255)
                 tCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAg                                                                                       >  1:351732/1‑101 (MQ=255)
                  cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                      <  2:5093212/101‑1 (MQ=255)
                   gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                     >  2:4209174/1‑101 (MQ=255)
                   gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                     <  2:1381072/101‑1 (MQ=255)
                    aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgagc                                                                                    >  2:939758/1‑101 (MQ=255)
                     aaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgcc                                                                                   <  2:602233/101‑1 (MQ=255)
                            ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.Gt                                                                          <  2:4048292/101‑1 (MQ=255)
                              cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCg                                                                        >  2:5312474/1‑101 (MQ=255)
                               gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGc                                                                       >  1:1968097/1‑101 (MQ=255)
                                tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCt                                                                      >  2:4783940/1‑101 (MQ=255)
                                  aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcc                                                                    <  2:6626703/101‑1 (MQ=255)
                                  aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcc                                                                    <  2:1272347/101‑1 (MQ=255)
                                      gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                                <  2:2963274/101‑1 (MQ=255)
                                      gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                                >  2:5992330/1‑101 (MQ=255)
                                        cAGTGATAGACGCCGTGGACGTCGCTCCACCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCcgc                                                              >  1:5070080/1‑101 (MQ=255)
                                              tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCTGGGGc                                                        <  1:5637625/101‑1 (MQ=255)
                                              tAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGc                                                        >  2:413786/1‑101 (MQ=255)
                                                gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                      <  1:2985900/101‑1 (MQ=255)
                                                gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                      >  2:1004433/1‑101 (MQ=255)
                                                 aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                     <  1:4520520/101‑1 (MQ=255)
                                                  gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                    <  1:3250742/91‑1 (MQ=9)
                                                   cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGt                                                      <  2:5580573/93‑1 (MQ=2)
                                                   ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                                   <  2:6134033/92‑1 (MQ=9)
                                                   ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                                   <  1:966489/92‑1 (MQ=9)
                                                    ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                     >  2:5462037/8‑101 (MQ=2)
                                                    ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                  <  1:4839583/101‑1 (MQ=255)
                                                    caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCCC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                  <  1:1768585/93‑1 (MQ=2)
                                                    caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                  <  2:2379022/93‑1 (MQ=9)
                                                     gcccggcGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                    <  2:1118135/95‑1 (MQ=2)
                                                     gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                    <  2:4605778/95‑1 (MQ=2)
                                                     cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                 >  2:2519661/1‑101 (MQ=255)
                                                     aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                 <  2:5118312/94‑1 (MQ=9)
                                                      gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  2:802660/1‑101 (MQ=255)
                                                      gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCCCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  1:3635253/1‑101 (MQ=255)
                                                       tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                               >  1:5753739/1‑101 (MQ=255)
                                                        ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                              <  1:1930642/101‑1 (MQ=255)
                                                         tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             >  2:3828038/4‑101 (MQ=9)
                                                         tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCACCGCGGGGGCGTGGATCGaaa                                             >  2:3183540/4‑101 (MQ=9)
                                                         ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  1:5879403/3‑101 (MQ=2)
                                                         ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                >  1:1100936/3‑101 (MQ=2)
                                                         gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             >  2:343072/1‑101 (MQ=255)
                                                          gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                               <  1:1155281/100‑1 (MQ=2)
                                                          atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            <  1:3108723/99‑1 (MQ=9)
                                                          aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                            <  1:6453250/101‑1 (MQ=255)
                                                           tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                           <  2:3554508/100‑2 (MQ=9)
                                                           cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                           <  2:1488475/101‑1 (MQ=255)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAACCGa                                          >  2:5731937/1‑101 (MQ=255)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                             <  1:2772260/101‑1 (MQ=2)
                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                         >  2:2632018/1‑101 (MQ=255)
                                                              cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                        >  2:5626397/1‑101 (MQ=9)
                                                              cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                        >  1:3744026/1‑101 (MQ=9)
                                                              cGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                        <  2:1659801/101‑1 (MQ=255)
                                                                cTCCCCCCGCGGGGGCGTGGATCGAACCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                      <  2:2026815/101‑1 (MQ=255)
                                                                 tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                     <  2:1050617/98‑2 (MQ=9)
                                                                   cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                   >  1:3796049/1‑101 (MQ=255)
                                                                   cccccGCGGGGGCGTGGATCGAAACTGCGCCCCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                   <  1:3309835/101‑1 (MQ=255)
                                                                    ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                  <  1:4209369/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                 <  2:3609009/101‑6 (MQ=9)
                                                                     cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                 <  1:1480303/101‑1 (MQ=255)
                                                                       cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                               <  2:700176/101‑8 (MQ=2)
                                                                        gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                              <  1:7043461/101‑9 (MQ=2)
                                                                          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                            <  2:6875624/101‑11 (MQ=2)
                                                                          gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                            <  1:6053588/101‑1 (MQ=255)
                                                                          gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                            >  1:5411603/1‑101 (MQ=255)
                                                                           ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                           >  1:5566782/1‑101 (MQ=255)
                                                                            gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                          <  1:2534940/101‑1 (MQ=255)
                                                                             ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                         <  1:344881/101‑1 (MQ=255)
                                                                             ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                         >  2:4402061/1‑101 (MQ=255)
                                                                             ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                         <  1:1652063/101‑1 (MQ=255)
                                                                              gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                        <  1:1002962/101‑1 (MQ=255)
                                                                               cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCGTTCATGGCCTACGTc                       >  1:6844405/1‑101 (MQ=255)
                                                                               cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                       <  1:4395873/101‑1 (MQ=255)
                                                                               cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                       >  2:4003795/1‑101 (MQ=255)
                                                                               cGTGGATCGAAACGGCGCCGCCTAGCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                       <  2:2234096/101‑1 (MQ=255)
                                                                                gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                      >  2:2677663/1‑101 (MQ=255)
                                                                                 tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                     >  1:650511/1‑101 (MQ=255)
                                                                                  ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                    >  1:1436211/1‑101 (MQ=255)
                                                                                       gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAggg               >  1:4735180/1‑99 (MQ=255)
                                                                                       gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg               <  1:5998090/101‑1 (MQ=255)
                                                                                          aCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGGGGAc            >  2:3361285/1‑101 (MQ=255)
                                                                                            tGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg          >  2:6440369/1‑101 (MQ=255)
                                                                                            cgcgcCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg          <  2:673069/100‑1 (MQ=255)
                                                                                              cgccgcCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt        <  2:1587478/101‑1 (MQ=255)
                                                                                                 cgccTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc     <  1:4661642/101‑1 (MQ=255)
                                                                                                  gccTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCCACGTCGGGCAGTGGACTggccact    >  2:1726214/1‑96 (MQ=255)
                                                                                                   ccTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc   <  2:4818445/101‑1 (MQ=255)
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CCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCC  >  NC_014034/1395772‑1395972

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG