Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,873 T→C 1.3% intergenic (+685/‑246) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8730T→C 1.3% 3.0 ‑0.3 ‑0.0461intergenic (+685/‑246)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (214/240):  total (218/243)
Fisher's exact test strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-01

Only 100 of 485 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCC  >  NC_014034/1395773‑1395973
                                                                                                    |                                                                                                           
  cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                           <  2:73984/101‑1 (MQ=255)
  cgcgGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                           <  2:6515780/101‑1 (MQ=255)
  cgcgGGGGCGTGGAGCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTAt                                                                                                           <  2:4252732/101‑1 (MQ=255)
      ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                                                                                       >  1:1821690/1‑101 (MQ=255)
      ggggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                                                                                                       >  1:4007822/1‑101 (MQ=255)
       gggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCAt                                                                                                      >  2:2985791/1‑101 (MQ=255)
        ggCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                                                                                                     <  2:5320124/101‑1 (MQ=255)
         gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                    <  1:4884305/101‑1 (MQ=255)
         gCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc                                                                                                    <  1:4055940/101‑1 (MQ=255)
           gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                                  >  1:1375471/1‑101 (MQ=255)
           gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                                  >  2:5997785/1‑101 (MQ=255)
           gTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg                                                                                                  >  2:5933265/1‑101 (MQ=255)
            tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...t                                                                                              >  1:3878181/1‑101 (MQ=255)
            gggATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...t                                                                                              >  2:6047636/2‑101 (MQ=255)
              gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCg                                                                                            <  2:5541839/101‑1 (MQ=255)
              gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCg                                                                                            <  2:3370767/101‑1 (MQ=255)
               aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGa                                                                                           <  1:6006397/101‑1 (MQ=255)
                 cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                         >  2:4525853/1‑101 (MQ=255)
                  gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcg                                                                                        >  2:5046733/1‑101 (MQ=255)
                   aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                       >  1:2365769/1‑101 (MQ=255)
                   aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgagc                                                                                       >  2:939758/1‑101 (MQ=255)
                   aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCCAgcgc                                                                                       >  2:5376513/1‑101 (MQ=255)
                      ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGcgc.cgc                                                                                   >  2:1920034/1‑101 (MQ=255)
                         gcggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.a                                                                              >  1:6889404/1‑101 (MQ=255)
                                gAGTTGCCAGTTATAGACGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTc                                                                      <  2:5688779/101‑1 (MQ=255)
                                gAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTc                                                                      <  2:1668703/101‑1 (MQ=255)
                                     gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTcccccc                                                                 <  1:2435171/101‑1 (MQ=255)
                                     gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTcccccc                                                                 <  2:2391465/101‑1 (MQ=255)
                                     gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTcccccc                                                                 >  1:3455718/1‑101 (MQ=255)
                                            aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCggggg                                                          >  2:5188918/1‑101 (MQ=255)
                                               gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGt                                                       >  2:1004433/1‑101 (MQ=255)
                                                aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTg                                                      <  1:4520520/101‑1 (MQ=255)
                                                 gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTgg                                                     <  1:3250742/91‑1 (MQ=9)
                                                 cGCGGTGGACGTCCCTCCCCCCGTGGGGGCGGGGATCGAACCTGCGCCACCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTgg                                                     <  2:4070035/101‑1 (MQ=38)
                                                 cGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTgg                                                     <  1:5341476/101‑1 (MQ=255)
                                                  gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                                    >  2:4661403/1‑101 (MQ=255)
                                                  gCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAGACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGa                                                    <  1:5085521/101‑1 (MQ=255)
                                                  ccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGa                                                    <  1:966489/92‑1 (MQ=9)
                                                   caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCGCC.C.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:1768585/93‑1 (MQ=2)
                                                   caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  1:5442037/9‑101 (MQ=9)
                                                   caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  1:3109116/9‑101 (MQ=9)
                                                    gcccggcGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCT.G.C.GTCGCTCCCCCCGCGGGGGCGTgg                                                     <  2:1118135/95‑1 (MQ=2)
                                                    cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                  >  2:2519661/1‑101 (MQ=255)
                                                     gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  2:802660/1‑101 (MQ=255)
                                                     gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  2:4813314/1‑101 (MQ=255)
                                                     gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  1:1492773/1‑101 (MQ=255)
                                                     gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGAGCg                                                 >  1:6725861/1‑101 (MQ=255)
                                                     gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CCCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  1:3635253/1‑101 (MQ=255)
                                                     acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  2:1310426/95‑1 (MQ=9)
                                                      tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  1:2679016/101‑1 (MQ=255)
                                                      cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGCCCTCGCG.C...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  2:3621060/96‑1 (MQ=9)
                                                       ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  1:1930642/101‑1 (MQ=255)
                                                       ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  2:3868151/101‑1 (MQ=255)
                                                        tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              >  2:5618808/4‑101 (MQ=9)
                                                        tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              >  2:6246849/4‑101 (MQ=9)
                                                        ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  2:5117031/3‑101 (MQ=2)
                                                        ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  1:1100936/3‑101 (MQ=2)
                                                        gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              <  2:1394385/101‑1 (MQ=255)
                                                        gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              >  2:343072/1‑101 (MQ=255)
                                                         gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGC.CGCT.G.C.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  1:1155281/100‑1 (MQ=2)
                                                         atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                             >  1:3583044/3‑101 (MQ=9)
                                                          tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                            <  2:3554508/100‑2 (MQ=9)
                                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                            <  1:5827852/101‑1 (MQ=255)
                                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                            >  2:3250990/1‑101 (MQ=255)
                                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                           >  1:285072/1‑101 (MQ=255)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                          >  2:2632018/1‑101 (MQ=255)
                                                            tCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                          <  1:6178001/101‑1 (MQ=255)
                                                             cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                         >  1:3744026/1‑101 (MQ=9)
                                                              gCTCCCCCCGGGGGGGCGGGGATCGAAACGGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                        <  2:1792732/101‑1 (MQ=255)
                                                               ctcgcgccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.CTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                       <  2:5855268/95‑1 (MQ=2)
                                                               cTCCCCCCGCGGGGGCGTGGATCGAACCTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                       <  2:2026815/101‑1 (MQ=255)
                                                                tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                      <  2:1050617/98‑2 (MQ=9)
                                                                 caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                     <  2:4147385/99‑3 (MQ=9)
                                                                  cccccGCGGGGGCGTGGATCGAAACTGCGCCCCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                    <  1:3309835/101‑1 (MQ=255)
                                                                   ccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgc                                   <  1:2019990/101‑5 (MQ=9)
                                                                     ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                 <  1:3811152/101‑1 (MQ=255)
                                                                      cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                <  2:700176/101‑8 (MQ=2)
                                                                       gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                               >  2:149507/1‑93 (MQ=2)
                                                                         gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.C.CTCG.C.ACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcct                             <  2:6390351/101‑11 (MQ=2)
                                                                         gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                             >  1:558831/1‑101 (MQ=255)
                                                                         gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                             <  2:666427/101‑1 (MQ=255)
                                                                           gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                           <  1:6521361/101‑1 (MQ=255)
                                                                             gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                         <  1:1002962/101‑1 (MQ=255)
                                                                              cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                        <  1:4187258/101‑1 (MQ=255)
                                                                              cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                        <  1:4395873/101‑1 (MQ=255)
                                                                              cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                        <  1:1655529/101‑1 (MQ=255)
                                                                              cGTGGATCGAAACGGCGCCGCCTAGCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                        <  2:2234096/101‑1 (MQ=255)
                                                                               gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                       >  1:2986004/1‑101 (MQ=255)
                                                                               gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                       >  2:2677663/1‑101 (MQ=255)
                                                                                 ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                     >  2:6819212/1‑101 (MQ=255)
                                                                                 ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                     >  1:1436211/1‑101 (MQ=255)
                                                                                 ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                     >  1:6454286/1‑101 (MQ=255)
                                                                                     cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                 <  1:92348/101‑1 (MQ=255)
                                                                                      gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAggg                >  1:4735180/1‑99 (MQ=255)
                                                                                       aaaCTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg               <  1:5723332/101‑1 (MQ=255)
                                                                                            gcgcCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg          >  2:4005149/1‑101 (MQ=255)
                                                                                             cgccgcCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt         <  2:1587478/101‑1 (MQ=255)
                                                                                                cgccTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc      <  1:4661642/101‑1 (MQ=255)
                                                                                                    tATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc  >  2:6867748/1‑101 (MQ=255)
                                                                                                    tATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc  >  1:5918936/1‑101 (MQ=255)
                                                                                                    |                                                                                                           
CCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGC.CGCG.C.A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCC  >  NC_014034/1395773‑1395973

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG