Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,887 G→T 7.8% intergenic (+699/‑232) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8870G→T 7.8% 81.1 ‑1.2 ‑0.3463intergenic (+699/‑232)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/22):  ref base (207/220):  total (221/242)
Fisher's exact test strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.42e-01

Only 100 of 487 total aligned reads displayed.

GATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395787‑1395987
                                                                                                       |                                                                                                      
 aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACTGCGCCGCCTATCGCATCCGG...TCGa                                                                                                       >  1:3409231/1‑101 (MQ=255)
 aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGa                                                                                                       >  1:666447/1‑101 (MQ=255)
 aTCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGa                                                                                                       <  1:4091486/101‑1 (MQ=255)
   cGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgc                                                                                                     >  2:4525853/1‑101 (MQ=255)
     aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                                   <  2:1425726/101‑1 (MQ=255)
     aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAgcgc                                                                                                   >  2:6606208/1‑101 (MQ=255)
     aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCCAgcgc                                                                                                   >  2:5376513/1‑101 (MQ=255)
          tGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCgcg.c                                                                                             <  1:421279/101‑1 (MQ=255)
                gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGc                                                                                      >  2:7073200/1‑101 (MQ=255)
                   aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcc                                                                                   >  2:2604770/1‑101 (MQ=255)
                      tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTccccc                                                                                >  1:1153256/1‑101 (MQ=255)
                       gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                                               <  2:2391465/101‑1 (MQ=255)
                       gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                                               >  2:5992330/1‑101 (MQ=255)
                       gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTcccccc                                                                               <  2:1683754/101‑1 (MQ=255)
                           gTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCgg                                                                           <  1:4678799/101‑1 (MQ=255)
                              aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCggggg                                                                        <  2:6824660/101‑1 (MQ=255)
                              aTAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCggggg                                                                        >  2:5188918/1‑101 (MQ=255)
                                aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                      >  2:1959954/1‑101 (MQ=255)
                                aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                      <  2:387930/101‑1 (MQ=255)
                                 gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                                     <  1:2985900/101‑1 (MQ=255)
                                  aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:4520520/101‑1 (MQ=255)
                                   gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  1:3782613/91‑1 (MQ=9)
                                   cGCGGTGGACGTCCCTCCCCCCGTGGGGGCGGGGATCGAACCTGCGCCACCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:4070035/101‑1 (MQ=38)
                                   cGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:4007647/101‑1 (MQ=255)
                                    gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  2:4661403/1‑101 (MQ=255)
                                    gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:1726573/1‑101 (MQ=255)
                                    gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  >  1:2419733/1‑101 (MQ=255)
                                    gCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAGACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                  <  1:5085521/101‑1 (MQ=255)
                                    cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGt                                                                     >  1:1967288/9‑101 (MQ=2)
                                     caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 >  2:2986494/9‑101 (MQ=9)
                                      cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  2:1627207/1‑101 (MQ=255)
                                      cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  2:351728/101‑1 (MQ=255)
                                       gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  2:4813314/1‑101 (MQ=255)
                                        cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  1:3514869/6‑101 (MQ=9)
                                        ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:6999633/97‑1 (MQ=2)
                                         ggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             >  1:4734872/1‑101 (MQ=255)
                                         ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  2:6255118/97‑1 (MQ=9)
                                         cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:4892822/98‑1 (MQ=2)
                                          tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCGCCC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  1:1899076/4‑101 (MQ=2)
                                          tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCACCGCGGGGGCGTGGATCGaaa                                                            >  2:3183540/4‑101 (MQ=9)
                                          ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  2:5117031/3‑101 (MQ=2)
                                          ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  1:5879403/3‑101 (MQ=2)
                                          ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  2:6689810/99‑1 (MQ=2)
                                           atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           <  1:3731231/99‑1 (MQ=9)
                                             gTCGCTCCCCTCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTCTGGTGCCGCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  2:4146182/101‑1 (MQ=2)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                         >  1:285072/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                         >  1:3103248/1‑101 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                        >  2:2632018/1‑101 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                        >  1:3979374/1‑101 (MQ=255)
                                              tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCAGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                        <  1:361773/101‑1 (MQ=2)
                                              tCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                        <  1:6178001/101‑1 (MQ=255)
                                              ccGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                        <  1:2110095/100‑1 (MQ=255)
                                               cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                       >  2:5626397/1‑101 (MQ=9)
                                               cGCACCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGCGGGCGTGGATCGAAACGATg                                                       >  1:5593616/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCCCGGGGGCGCGGATCGAAACGAGGCg                                                     >  1:1793627/1‑101 (MQ=255)
                                                  tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                    <  1:322104/98‑2 (MQ=9)
                                                   caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                                   >  2:92874/3‑99 (MQ=9)
                                                    cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                  <  1:1335313/101‑1 (MQ=255)
                                                    accccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcg                                                  >  2:1042178/2‑98 (MQ=9)
                                                     ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                 <  2:6411699/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                 <  1:3267562/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TGGGGC.CCTCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                                <  2:3609009/101‑6 (MQ=9)
                                                      cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                <  1:1100342/101‑1 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                               >  1:1623534/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGc                                             >  1:2533596/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                            >  1:3851113/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                          <  1:965578/101‑1 (MQ=255)
                                                              ggCGGGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                        <  1:5361781/101‑1 (MQ=255)
                                                               gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                       <  1:1002962/101‑1 (MQ=255)
                                                                cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCGTTCATGGCCTACGTc                                      >  1:6844405/1‑101 (MQ=255)
                                                                cGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                      <  1:4395873/101‑1 (MQ=255)
                                                                cGTGGATCGAAACGGCGCCGCCTAGCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                      <  2:2234096/101‑1 (MQ=255)
                                                                 gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                     >  2:2677663/1‑101 (MQ=255)
                                                                  tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                    >  1:5784231/1‑101 (MQ=255)
                                                                  tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                    >  1:650511/1‑101 (MQ=255)
                                                                   ggATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                   >  1:6454286/1‑101 (MQ=255)
                                                                       cGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                               <  1:5521155/101‑1 (MQ=255)
                                                                        gAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                              <  1:5998090/101‑1 (MQ=255)
                                                                          aaCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                            >  2:1819605/1‑101 (MQ=255)
                                                                           aCTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGGGGAc                           >  2:3361285/1‑101 (MQ=255)
                                                                            cTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGCCt                          >  2:788534/1‑101 (MQ=255)
                                                                              gcgcCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                        >  1:990790/1‑101 (MQ=255)
                                                                                gccgccTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGCTc                      <  2:1541448/101‑1 (MQ=255)
                                                                                   gccTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                   >  1:5724373/1‑101 (MQ=255)
                                                                                      tATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                <  2:585777/101‑1 (MQ=255)
                                                                                      tATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                >  2:6039829/1‑101 (MQ=255)
                                                                                       aTCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccag               >  1:862994/1‑99 (MQ=255)
                                                                                        tCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc              <  2:1153208/101‑1 (MQ=255)
                                                                                        tCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc              <  2:551582/101‑1 (MQ=255)
                                                                                         cGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc             <  2:4385109/101‑1 (MQ=255)
                                                                                         cGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc             <  2:6874831/101‑1 (MQ=255)
                                                                                            aTCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg          >  2:2037792/1‑101 (MQ=255)
                                                                                            aTCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg          >  2:1351248/1‑101 (MQ=255)
                                                                                               cGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg       >  1:1394517/1‑101 (MQ=255)
                                                                                               cGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg       >  2:3428840/1‑101 (MQ=255)
                                                                                                 g...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc     <  2:5783929/101‑1 (MQ=255)
                                                                                                 g...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc     <  2:558819/101‑1 (MQ=255)
                                                                                                 g...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc     >  1:1470041/1‑101 (MQ=255)
                                                                                                 g...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCCCcggggg     >  2:5029284/1‑100 (MQ=255)
                                                                                                 g...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCGCCCCCCGCGGGGGc     <  2:3696398/101‑1 (MQ=255)
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GATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TCGAGCGCCGCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395787‑1395987

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG