BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,931	G→A	9.3%	intergenic (+743/‑188)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,931	0	G→A	9.3%	89.1 ‑1.8 ‑0.5	402	intergenic (+743/‑188)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (15/22): ref base (186/167): total (204/198)
Fisher's exact test strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.34e-01

Only 100 of 426 total aligned reads displayed.


CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGC  >  NC_014034/1395831‑1396031


cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                                                      <  1:5827852/101‑1 (MQ=255)


 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                                                                     >  2:438729/1‑101 (MQ=255)


   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                                                                   >  2:1068601/1‑101 (MQ=255)


   cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                                                                   >  1:3744026/1‑101 (MQ=9)


     cTCCCCCCGCGGGGGCGTGGATCGAAACTGNGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                                                                 <  1:3192165/101‑1 (MQ=255)


      tCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                                                                                >  1:4816346/1‑101 (MQ=255)


      acaccccGCGGGGGCGTGGATCGAAACTGTTCCGGTTTCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                                                                <  2:822553/98‑2 (MQ=9)


       caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                                                                               >  2:92874/3‑99 (MQ=9)


       caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                                                                               <  1:4098885/99‑3 (MQ=9)


       caccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggc                                                                                               >  1:596005/3‑99 (MQ=9)


        cccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                                                              <  1:1335313/101‑1 (MQ=255)


         ccccGGGGGGGCGGGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                             <  1:4169571/101‑1 (MQ=255)


          cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                            <  2:4970153/101‑1 (MQ=255)


           ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                                                                           <  1:3811152/101‑1 (MQ=255)


           ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                                                                           >  1:1623534/1‑101 (MQ=255)


            cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                          <  2:3635104/101‑1 (MQ=255)


             gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                                                         >  2:149507/1‑93 (MQ=2)


             gcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGc                                                                                         >  1:2533596/1‑101 (MQ=255)


              cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                                                                        >  1:1394390/1‑92 (MQ=2)


              cgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                                                                        <  1:6222779/101‑10 (MQ=2)


               gggggCGTGGATCGAAACTGCGCCGCCTATCGTATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                       <  2:3896832/101‑1 (MQ=255)


               gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                       >  1:5411603/1‑101 (MQ=255)


                ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                                                                      >  1:5566782/1‑101 (MQ=255)


                ggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                                                                      >  1:4641695/1‑101 (MQ=255)


                 gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                     <  1:2713429/101‑1 (MQ=255)


                 gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                     <  1:6521361/101‑1 (MQ=255)


                 gggCGCTGATCGAACCTGCCCCGCCTGCCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCttc                                                                                     <  2:6725519/101‑3 (MQ=25)


                  ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                                                                    <  1:344881/101‑1 (MQ=255)


                  ggCGGGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                                                                    <  1:5361781/101‑1 (MQ=255)


                   gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                                                                   <  1:1002962/101‑1 (MQ=255)


                    cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                  >  2:4003795/1‑101 (MQ=255)


                    cGTGGATCGAAACGGCGCCGCCTAGCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                  <  2:2234096/101‑1 (MQ=255)


                      tGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                                                                >  1:2071116/1‑101 (MQ=255)


                      tGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                                                                >  1:6465995/1‑101 (MQ=255)


                       ggATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                                                               >  2:5116902/1‑101 (MQ=255)


                             aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                                                         >  2:6382178/1‑101 (MQ=255)


                             aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                                                         <  1:6047948/101‑1 (MQ=255)


                             aaaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                                                         <  2:6448291/101‑1 (MQ=255)


                              aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                        <  1:1209070/101‑1 (MQ=255)


                              aaCTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGa                                                                        >  2:1819605/1‑101 (MQ=255)


                                   cgccgcCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                   <  2:2627623/101‑1 (MQ=255)


                                    gccgccTCGCCCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                  <  2:3296919/101‑1 (MQ=255)


                                       gccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCCACGTCGGGCAGTGGACTggccact                                                               >  2:1726214/1‑96 (MQ=255)


                                         cTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                                                             >  2:3061942/1‑101 (MQ=255)


                                          tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                            >  2:6039829/1‑101 (MQ=255)


                                          tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                            <  2:585777/101‑1 (MQ=255)


                                          tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                            >  2:6867748/1‑101 (MQ=255)


                                           aTCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                                                           >  1:5727693/1‑101 (MQ=255)


                                             cGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGTGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                         <  1:186387/101‑1 (MQ=255)


                                             cGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                         <  2:3706883/101‑1 (MQ=255)


                                             cGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                         <  1:4250508/101‑1 (MQ=255)


                                               cATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc                                                       >  2:6636909/1‑101 (MQ=255)


                                               cATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc                                                       >  1:2303630/1‑101 (MQ=255)


                                                aTCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg                                                      <  1:1068636/101‑1 (MQ=255)


                                                aTCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg                                                      >  1:5774336/1‑101 (MQ=255)


                                                aTCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgcg                                                      >  2:1351248/1‑101 (MQ=255)


                                                   cGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg                                                   >  1:6750848/1‑101 (MQ=255)


                                                   cGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg                                                   >  2:3428840/1‑101 (MQ=255)


                                                    ggTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCCGTGGACCGGTCCCTCCCCCCGCggggg                                                  >  2:7005212/1‑101 (MQ=255)


                                                     gTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                 >  1:1470041/1‑101 (MQ=255)


                                                     gTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                 <  1:987545/101‑1 (MQ=255)


                                                     gTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                 <  2:558819/101‑1 (MQ=255)


                                                     gTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc                                                 <  2:5783929/101‑1 (MQ=255)


                                                       cGAGCGCCGCGCA.GTCGCTCACCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                               <  1:6835152/101‑1 (MQ=255)


                                                        gAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCggg                                              >  2:4143612/1‑99 (MQ=255)


                                                        gAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg                                              >  2:3550750/1‑101 (MQ=255)


                                                         aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:2678021/101‑1 (MQ=255)


                                                         aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTgg                                             <  1:4003970/101‑1 (MQ=255)


                                                           cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:1621360/101‑1 (MQ=255)


                                                           cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCCCGGGGGCGTGGAt                                           >  1:5112668/1‑101 (MQ=255)


                                                            gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:2009557/1‑101 (MQ=255)


                                                            gccgcGCA.GTCACTCCCCCCGCGGGGGCGTGGATCGAAACGTTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGAGGATc                                          >  2:1651092/1‑101 (MQ=255)


                                                             ccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGCCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  2:2893731/1‑101 (MQ=255)


                                                              cgcgCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:313041/1‑101 (MQ=255)


                                                              cgcgCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  1:2632114/101‑1 (MQ=255)


                                                               gcgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  1:4526092/101‑1 (MQ=255)


                                                                cgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  2:3376186/101‑1 (MQ=255)


                                                                 ggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      <  1:2983940/97‑1 (MQ=2)


                                                                   a.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTc                                   <  1:3389266/101‑1 (MQ=255)


                                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCg                                  >  2:1939928/1‑101 (MQ=255)


                                                                           ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                            >  2:7120563/1‑101 (MQ=255)


                                                                             ccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGAt                          <  1:6630343/101‑1 (MQ=255)


                                                                                 gcgGGGGCTTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgc                      >  1:2856754/1‑101 (MQ=255)


                                                                                 gcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgc                      >  1:3056853/1‑101 (MQ=255)


                                                                                      ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                 <  1:3885462/101‑1 (MQ=255)


                                                                                      ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                 <  2:5411331/101‑1 (MQ=255)


                                                                                        cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc               >  2:5664906/1‑101 (MQ=255)


                                                                                        cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGGCCCCCGATCCGCGGttcgc               >  2:6938977/1‑98 (MQ=38)


                                                                                         gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCt              >  1:4718238/1‑101 (MQ=255)


                                                                                          tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc             <  1:364378/101‑1 (MQ=255)


                                                                                          tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc             >  2:4453177/1‑101 (MQ=255)


                                                                                          tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc             <  1:802684/101‑1 (MQ=255)


                                                                                            gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCAc           <  1:1659987/101‑1 (MQ=255)


                                                                                             aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg          >  2:2029636/1‑101 (MQ=255)


                                                                                             aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg          >  1:95994/1‑101 (MQ=255)


                                                                                              tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc         <  1:4377407/101‑1 (MQ=255)


                                                                                                gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt       <  2:2303535/101‑1 (MQ=255)


                                                                                                gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCt       >  2:472833/1‑101 (MQ=255)


                                                                                                 aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg      <  1:3251106/101‑1 (MQ=255)


                                                                                                     gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCcgc  >  1:6987788/1‑101 (MQ=255)


CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGC  >  NC_014034/1395831‑1396031

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG