Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,013 C→T 12.4% intergenic (+825/‑106) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0130C→T12.4% 130.0 ‑1.2 ‑0.2402intergenic (+825/‑106)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/30):  ref base (172/180):  total (192/210)
Fisher's exact test strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.97e-01

Only 100 of 426 total aligned reads displayed.

GGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCG  >  NC_014034/1395913‑1396113
                                                                                                    |                                                                                                         
   ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                                                                                                        <  1:2037872/101‑1 (MQ=255)
     cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGGCCCCCGATCCGCGGttcgc                                                                                                      >  2:6938977/1‑98 (MQ=38)
      gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..t                                                                                                   <  2:2364364/101‑1 (MQ=255)
      gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..t                                                                                                   >  1:4718238/1‑101 (MQ=255)
      gTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..t                                                                                                   >  2:6637038/1‑101 (MQ=255)
       tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..Tc                                                                                                  <  1:802684/101‑1 (MQ=255)
       tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..Tc                                                                                                  <  1:1351299/101‑1 (MQ=255)
       tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..Tc                                                                                                  >  1:1515790/1‑101 (MQ=255)
        gaaTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCCGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCa                                                                                                 <  2:3445220/99‑1 (MQ=255)
         gATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCAc                                                                                                <  1:1659987/101‑1 (MQ=255)
          aTCGAAACTATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACg                                                                                               >  1:3153788/1‑101 (MQ=255)
           tCGAGACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCttagatcggaa                                                                                                >  2:3937966/1‑92 (MQ=38)
           tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGc                                                                                              <  1:3809051/101‑1 (MQ=255)
            cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGcc                                                                                             >  2:7095377/1‑101 (MQ=255)
            cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGcc                                                                                             >  2:6022462/1‑101 (MQ=255)
            cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTAGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGcc                                                                                             >  2:1460058/1‑101 (MQ=255)
                aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGc.c                                                                                        <  1:1004468/101‑1 (MQ=255)
                aCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGc.c                                                                                        >  2:3342293/1‑101 (MQ=255)
                 cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.cg                                                                                       >  1:3960918/1‑101 (MQ=255)
                  gATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.cgc                                                                                      >  1:5782165/1‑101 (MQ=255)
                   aTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.cgc..g                                                                                   >  2:1925780/1‑101 (MQ=255)
                     gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTc                                                                                 <  1:5874747/101‑1 (MQ=255)
                     gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTc                                                                                 >  2:1478174/1‑101 (MQ=255)
                         tCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTc                                                                             >  2:4524200/1‑101 (MQ=255)
                          cATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTcc                                                                            <  1:5960634/101‑1 (MQ=255)
                           aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCCCGCCTGC.CGC..GGCGCGccc                                                                           >  2:5710632/1‑101 (MQ=255)
                           aTGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTccc                                                                           >  1:3612376/1‑101 (MQ=255)
                             ggCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTccccc                                                                         >  2:3624953/1‑101 (MQ=255)
                              gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTcccccc                                                                        <  1:5732239/101‑1 (MQ=255)
                              gCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTcccccc                                                                        <  2:2533502/101‑1 (MQ=255)
                                cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                      <  1:3062055/101‑1 (MQ=255)
                                    gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                  >  2:2811551/1‑101 (MQ=255)
                                    gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                  >  1:921094/1‑101 (MQ=255)
                                       gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                               <  2:4436603/101‑1 (MQ=255)
                                         gCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTg                                                             <  1:1754565/101‑1 (MQ=255)
                                          cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                            <  1:3550886/101‑1 (MQ=255)
                                            gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                          <  2:4176549/101‑1 (MQ=255)
                                             tGGGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:461633/101‑1 (MQ=255)
                                             tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:5753437/101‑1 (MQ=255)
                                             tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:5552977/101‑1 (MQ=255)
                                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:862212/96‑1 (MQ=9)
                                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         >  1:3361709/6‑101 (MQ=9)
                                              tgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:6568922/96‑1 (MQ=9)
                                                aCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  1:343076/101‑1 (MQ=255)
                                                 gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  1:4229873/99‑1 (MQ=9)
                                                 gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  1:2729651/99‑1 (MQ=2)
                                                 gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  2:5381853/99‑1 (MQ=9)
                                                  tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    <  2:6520297/101‑1 (MQ=255)
                                                  cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     <  2:5817256/100‑1 (MQ=9)
                                                  cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     >  1:5021249/2‑101 (MQ=9)
                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCNGGGGCGTGGATCGAGAc                                                    >  2:2953439/1‑101 (MQ=9)
                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    >  2:4070645/1‑101 (MQ=9)
                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                   >  1:3242319/1‑101 (MQ=255)
                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                   <  2:1386936/101‑1 (MQ=255)
                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                   <  1:4402283/101‑1 (MQ=255)
                                                   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                   >  2:4377690/1‑101 (MQ=255)
                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                 >  2:516018/1‑98 (MQ=2)
                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgc                                                <  2:5593326/101‑1 (MQ=255)
                                                      cGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCCCGTATCGCGCGC.TG...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                 <  2:3007283/101‑4 (MQ=2)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcg                                               >  1:6099417/1‑101 (MQ=255)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTACCCCCGCGGGGGCGTGGATCGAAACAGgcg                                               >  1:5101054/1‑101 (MQ=255)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                              >  1:540394/1‑101 (MQ=255)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                             <  1:6826053/101‑1 (MQ=255)
                                                          ccacccGCGGGGGCGTGGATAGAAACTCGACCTCGATCCGCGCTTGCC..TCACGCCTGA.CGC..GTGGCTCCCCCGGCGGGGGGGTGGATCGAAACGGGGGCAt                                            >  2:2276958/4‑101 (MQ=255)
                                                            ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCC..TC.GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                           >  2:6139268/1‑92 (MQ=2)
                                                             cccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTg                                         <  1:4153399/101‑1 (MQ=255)
                                                                 cgGGGGCGGGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGAttt                                     <  2:1155289/101‑3 (MQ=255)
                                                                  gggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                    >  2:783064/1‑101 (MQ=255)
                                                                   ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                   <  1:6637251/101‑1 (MQ=255)
                                                                      gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                <  2:327490/101‑1 (MQ=255)
                                                                       cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                               <  2:6804137/101‑1 (MQ=255)
                                                                       cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                               >  1:703666/1‑101 (MQ=255)
                                                                        gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                              >  2:6374261/1‑101 (MQ=255)
                                                                        gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                              >  1:1170616/1‑101 (MQ=255)
                                                                          ggATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                            >  1:710324/1‑101 (MQ=255)
                                                                          ggATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGAGCATCCGCTGGATACGCCCg                            >  2:5434433/1‑101 (MQ=37)
                                                                           gATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCgg                           >  2:6266280/1‑101 (MQ=255)
                                                                            aTCGAAGCCCGCCCTCGGTCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGt                          <  1:1955214/101‑1 (MQ=38)
                                                                               gAACCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                       <  1:983391/101‑1 (MQ=255)
                                                                               gAACCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                       <  2:1134257/101‑1 (MQ=255)
                                                                               gAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                       >  1:4311971/1‑101 (MQ=255)
                                                                               gAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                       >  2:4945005/1‑101 (MQ=255)
                                                                               gAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                       <  1:2911656/101‑1 (MQ=255)
                                                                                aaaCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                      <  1:4524439/101‑1 (MQ=255)
                                                                                aaaCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgaga                      >  1:5219885/1‑101 (MQ=255)
                                                                                 accTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                     <  2:3795888/99‑1 (MQ=255)
                                                                                 aaCTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                     >  2:2683769/1‑101 (MQ=255)
                                                                                    tCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGc                  >  1:6302235/1‑101 (MQ=255)
                                                                                      gCCCCCGGTCCGCGTTTGCC..TCTCGCCTGC.CTC..GTCGCGCCCCCCGCGGGGGCGTGGATCTAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTg                <  1:2277053/101‑1 (MQ=37)
                                                                                       cccTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt               <  2:2071036/101‑1 (MQ=255)
                                                                                       cccTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt               <  2:3153675/101‑1 (MQ=255)
                                                                                         cTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCg             >  2:1634525/1‑101 (MQ=255)
                                                                                         cTCGAGCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCg             <  2:4725299/101‑1 (MQ=255)
                                                                                            gATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc          <  2:4734632/101‑1 (MQ=255)
                                                                                             aTCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCTGGTGAGACGGCTGTCGCTcc         <  1:5491887/101‑1 (MQ=255)
                                                                                               ccGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc       <  2:3712472/101‑1 (MQ=255)
                                                                                                 gcgcTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc     >  2:4668194/1‑101 (MQ=255)
                                                                                                  cgcTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcg    <  1:2519755/101‑1 (MQ=255)
                                                                                                    cTTGCC..TCCCGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg  <  1:6939350/101‑1 (MQ=255)
                                                                                                    cTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgcg  >  1:2348665/1‑101 (MQ=255)
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GGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCC..TCACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCG  >  NC_014034/1395913‑1396113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG