Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,154 G→A 2.4% A12A (GCG→GTA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1540G→A 2.4% 7.0 0.0 ‑0.1289A12A (GCG→GCA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (145/137):  total (149/140)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.85e-01

Only 100 of 373 total aligned reads displayed.

GGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396054‑1396254
                                                                                                    |                                                                                                    
ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTcgcg                                                                                                      >  1:3042038/1‑101 (MQ=255)
  aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGcgcc                                                                                                    <  2:896225/101‑1 (MQ=255)
   tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGcgccc                                                                                                   >  2:1130847/1‑100 (MQ=255)
     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccgcc                                                                                                 <  1:6168175/101‑1 (MQ=255)
     gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccccc                                                                                                 >  2:435996/1‑101 (MQ=255)
       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACTGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCAc                                                                                               >  1:3453850/1‑101 (MQ=255)
        aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCAcc                                                                                              >  2:755757/1‑100 (MQ=255)
        aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACg                                                                                              >  2:956214/1‑101 (MQ=255)
        aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACg                                                                                              >  2:6553864/1‑101 (MQ=255)
         cAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGc                                                                                             >  2:4060873/1‑101 (MQ=255)
          aGGCGCATCTGATGTATATGCCCGGTGAGACGTCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCt                                                                                            <  2:5173014/101‑1 (MQ=255)
          aGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCCCGCt                                                                                            >  2:7091505/1‑101 (MQ=255)
           ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCgccccccccccg                                                                                           >  1:1135623/1‑95 (MQ=255)
           ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTg                                                                                           <  2:211272/101‑1 (MQ=255)
           ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCCCGCCGCCACGCTg                                                                                           >  2:4573815/1‑101 (MQ=255)
              gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCgc                                                                                        >  2:4729984/1‑100 (MQ=255)
               cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTc                                                                                       <  2:678125/101‑1 (MQ=255)
                ctCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCg                                                                                      <  2:3119025/100‑1 (MQ=255)
                aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCg                                                                                      <  1:2255429/101‑1 (MQ=255)
                aTCTGATGTATATGCCCGGTGAGACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTGCGTCATCTGGCAGGCTCGCGCCGCCCCCCTGCGTCg                                                                                      >  1:6280647/1‑101 (MQ=37)
                   tGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGGGCCGCCCCGCTGGGGCGCTc                                                                                   >  2:5962266/1‑101 (MQ=38)
                   tGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTc                                                                                   >  1:4783265/1‑101 (MQ=255)
                    gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTcc                                                                                  <  1:926654/101‑1 (MQ=255)
                       gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTccccc                                                                               <  1:1389788/101‑1 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGGTCGGCCCGCCCCGCTGCGTCGCTcccccc                                                                              >  1:3261377/1‑101 (MQ=38)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                              <  1:849438/101‑1 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACCCTGCGTCGCCcccccc                                                                              >  1:1637497/1‑101 (MQ=255)
                                cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                      >  2:4833908/1‑101 (MQ=255)
                                  gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCgg                                                                    >  1:5152721/1‑100 (MQ=255)
                                  gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCgg                                                                    >  1:5740862/1‑100 (MQ=255)
                                  gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCgg                                                                    >  2:1666135/1‑100 (MQ=255)
                                  gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGt                                                                    <  1:5839017/101‑1 (MQ=255)
                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCggg                                                                   >  1:3987700/1‑99 (MQ=255)
                                   tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCCCCCCCCGCGGGGGCGTg                                                                   >  2:4020239/1‑101 (MQ=255)
                                     agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTggg                                                                 >  1:6513682/1‑100 (MQ=255)
                                     agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGa                                                                 >  2:1548077/1‑101 (MQ=255)
                                       aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:285068/101‑1 (MQ=255)
                                       aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:2230564/101‑1 (MQ=255)
                                       aCGGCTGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  2:6744022/101‑1 (MQ=255)
                                        cGGCTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:2945419/101‑1 (MQ=255)
                                         gccTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  1:54668/99‑1 (MQ=255)
                                            tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAc                                                          >  2:5207223/1‑101 (MQ=255)
                                            tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          <  2:5752907/101‑1 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                         >  1:2757493/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGATCCCCCCGCGGGGGCGTGGATCGAAAcc                                                         >  1:4964151/1‑101 (MQ=255)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGGCGCCACCCCCGCGGGGGCGGGGATCCAAAccc                                                        >  2:2019608/1‑101 (MQ=38)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                                       <  1:1946835/101‑1 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                                       >  1:5301632/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCgg                                                      >  2:6650652/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCgg                                                      >  1:1121218/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCggg                                                     >  2:1391201/1‑100 (MQ=255)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCg                                                    >  2:2568470/1‑101 (MQ=255)
                                                  cccccccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCg                                                    <  1:3625102/100‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACCCGGcca                                                   >  2:4662493/1‑99 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                                                <  1:7063053/101‑1 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                                              <  1:2436491/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                            <  1:6764529/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGCAAAACGGc                                           >  1:2560046/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGc                                           >  1:6620093/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACCGGCGAAAAACGGc                                           >  2:2671521/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                                          >  1:4014712/1‑101 (MQ=255)
                                                             gggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                                         >  1:6070722/1‑101 (MQ=255)
                                                             gggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACCGGCGAAAAACGGCCa                                         >  2:118818/1‑101 (MQ=255)
                                                              ggCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                        <  1:2316481/101‑1 (MQ=255)
                                                               gCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                                       <  1:7416/101‑1 (MQ=255)
                                                               gCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                                       >  1:4569599/1‑101 (MQ=255)
                                                                cGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGGGGGGGTCGAAAACCGGCGAAAAACgcacaagc                                      >  2:3284930/1‑94 (MQ=255)
                                                                 gTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGcc                                     >  2:3022634/1‑101 (MQ=255)
                                                                 gTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGcc                                     >  1:1234484/1‑101 (MQ=255)
                                                                  tGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                                    >  2:633708/1‑101 (MQ=255)
                                                                   ggATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                   >  2:1848273/1‑101 (MQ=255)
                                                                   ggATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                   >  2:3866757/1‑101 (MQ=255)
                                                                   ggATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                   >  2:4212069/1‑101 (MQ=255)
                                                                    gATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                                  <  1:5611811/101‑1 (MQ=255)
                                                                    gATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTcc                                  >  2:6900383/1‑101 (MQ=255)
                                                                     aTCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                                 >  1:6965505/1‑101 (MQ=255)
                                                                     aTCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                                 >  1:6343960/1‑101 (MQ=255)
                                                                         aaaCTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggcag                             >  2:5433234/1‑98 (MQ=255)
                                                                          aaCTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgtgt                            <  2:5506430/101‑1 (MQ=255)
                                                                             ttttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                         <  1:4092422/101‑1 (MQ=255)
                                                                              tttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                        <  2:5134264/101‑1 (MQ=255)
                                                                              tttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                        <  2:5807012/101‑1 (MQ=255)
                                                                               ttCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                       <  2:1009000/101‑1 (MQ=255)
                                                                                   tCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCCTGACTCCGGGAGGCTGGCTGTc                   >  2:4996167/1‑101 (MQ=37)
                                                                                   gcATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                   <  1:1503849/100‑1 (MQ=255)
                                                                                    cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                  >  2:1284293/1‑101 (MQ=255)
                                                                                    cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                  <  1:6678223/101‑1 (MQ=255)
                                                                                    cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                  <  1:5665196/101‑1 (MQ=255)
                                                                                    cATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                  >  1:2819977/1‑101 (MQ=255)
                                                                                       cTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTc               >  2:7123785/1‑101 (MQ=255)
                                                                                       cTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTc               <  1:4326626/101‑1 (MQ=255)
                                                                                       cTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTCGCTc               >  2:2358473/1‑101 (MQ=255)
                                                                                        tGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc              >  1:1834492/1‑101 (MQ=255)
                                                                                        tGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc              <  1:3790629/101‑1 (MQ=255)
                                                                                        tGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc              <  1:3909026/101‑1 (MQ=255)
                                                                                        tGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc              >  1:4691726/1‑101 (MQ=255)
                                                                                        tGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGCCTGGCTGTCGCTcc              >  2:4444238/1‑101 (MQ=255)
                                                                                             ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcg         <  2:3699392/101‑1 (MQ=255)
                                                                                                    gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc  <  2:6202523/101‑1 (MQ=255)
                                                                                                    gccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc  >  1:1024453/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTCATCTGGCAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396054‑1396254

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG