Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,808 G→A 12.1% V230V (GTG→GTA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8080G→A12.1% 127.2 ‑2.3 ‑0.7420V230V (GTG→GTARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/33):  ref base (178/189):  total (197/223)
Fisher's exact test strand distribution p-value = 9.89e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.23e-01

Only 100 of 449 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCT  >  NC_014034/1396708‑1396908
                                                                                                    |                                                                                                        
cgCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGtgtg                                                                                                          <  2:3597779/101‑1 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTgg                                                                                                         >  1:3514641/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTgg                                                                                                         <  2:5355827/101‑1 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGga                                                                                                        >  1:2824741/1‑101 (MQ=255)
  cTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGga                                                                                                        >  1:6399563/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGgat...g                                                                                                   >  2:787930/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGgat...g                                                                                                   <  1:4604564/101‑1 (MQ=255)
     cccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGgat...ga                                                                                                  <  1:1193687/101‑1 (MQ=255)
      ccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGgat...gat                                                                                                 <  1:6338603/101‑1 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATc                                                                                                >  2:2195983/1‑101 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATc                                                                                                <  1:6142529/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCa                                                                                               >  2:3927238/1‑101 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCa                                                                                               >  1:2139688/1‑101 (MQ=255)
         cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                              <  2:5626280/101‑1 (MQ=255)
          gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATg                                                                                             <  2:1639152/101‑1 (MQ=255)
               ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGGGGAT...GATCATGCAgcg                                                                                        >  1:2735287/1‑101 (MQ=255)
                  gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.gg                                                                                    <  2:3310714/101‑1 (MQ=255)
                    ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGAc                                                                                  <  1:5442324/101‑1 (MQ=255)
                     gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACg                                                                                 >  1:5300604/1‑101 (MQ=255)
                      aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACgg                                                                                >  2:1794341/1‑101 (MQ=255)
                        cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCt                                                                              >  2:7090942/1‑101 (MQ=255)
                        cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCt                                                                              >  2:3665316/1‑101 (MQ=255)
                         gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACNNNNNGGAT...GATCATGCAGCGC.GGACGGCtt                                                                             >  1:3780948/1‑101 (MQ=255)
                         gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCtt                                                                             <  1:1398313/101‑1 (MQ=255)
                         gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCtt                                                                             >  1:1883686/1‑101 (MQ=255)
                         gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCtt                                                                             <  1:3319268/101‑1 (MQ=255)
                         gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCtt                                                                             >  1:2477787/1‑101 (MQ=255)
                          aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTg                                                                            >  2:2640464/1‑101 (MQ=255)
                             cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGt                                                                         <  2:471235/101‑1 (MQ=255)
                              ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTc                                                                        >  1:2407623/1‑101 (MQ=255)
                                 gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTTGAT...GATCATGCAGCGC.GGACGGCTTGGGTCACt                                                                     <  2:3045834/101‑1 (MQ=255)
                                 gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCt                                                                     >  2:12073/1‑101 (MQ=255)
                                   cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGCCGCTcc                                                                   >  1:862614/1‑101 (MQ=255)
                                    ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGGGGCTccc                                                                  >  2:3386721/1‑101 (MQ=255)
                                     cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTcccc                                                                 >  1:5715313/1‑101 (MQ=255)
                                      tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTccccc                                                                >  2:4358547/1‑101 (MQ=255)
                                      tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTccccc                                                                <  2:6043037/101‑1 (MQ=255)
                                       cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTcccccc                                                               <  2:2030636/101‑1 (MQ=255)
                                        atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCcg                                                              <  2:6815936/101‑1 (MQ=255)
                                         tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCcgc                                                             <  1:4693901/101‑1 (MQ=255)
                                          atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACCGTGTGGAA...GAGCATGCAGCGCGGGCCGGCTGGGCGGGCCCCCCCcgc                                                             >  1:6939150/1‑101 (MQ=21)
                                              tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCggggg                                                        >  1:2655938/1‑101 (MQ=255)
                                               gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCgggggg                                                       >  2:4165758/1‑100 (MQ=255)
                                               gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                       <  2:2809696/101‑1 (MQ=255)
                                               gcCACGATGCCCTTGAGTCGCTCCCCCCGAGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                       <  1:4506709/101‑1 (MQ=255)
                                                   cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                   <  1:5926912/101‑1 (MQ=255)
                                                    gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                  >  2:3441515/1‑101 (MQ=255)
                                                    gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.TGCCGGCTTGGGTCGCGCCCCCCGCGGGGGCGTGGa                                                  <  2:5282494/101‑1 (MQ=255)
                                                      tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                >  1:4035492/1‑101 (MQ=255)
                                                      cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                  >  2:6253356/10‑101 (MQ=0)
                                                        cccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  2:382697/1‑101 (MQ=255)
                                                        cccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              <  1:3252620/101‑1 (MQ=255)
                                                        catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:1521710/94‑1 (MQ=2)
                                                         ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  1:1619525/1‑101 (MQ=255)
                                                         ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  1:2011016/1‑101 (MQ=255)
                                                         ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  2:5465890/1‑101 (MQ=255)
                                                         atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  2:2597386/95‑1 (MQ=2)
                                                          tcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  1:3899652/6‑101 (MQ=2)
                                                           ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           <  2:1534799/101‑1 (MQ=255)
                                                           cgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  2:7034239/5‑101 (MQ=2)
                                                            tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                          >  1:5991069/1‑101 (MQ=255)
                                                            tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                          >  1:5471299/1‑101 (MQ=255)
                                                            gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:4858846/98‑1 (MQ=2)
                                                             gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                         <  2:6399239/101‑1 (MQ=255)
                                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                          <  1:4396926/100‑2 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                        <  1:1003942/101‑4 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                      >  2:3099625/1‑101 (MQ=255)
                                                                 ggCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                     <  1:4288006/100‑1 (MQ=255)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                       >  1:308688/1‑97 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                     <  2:3732564/101‑1 (MQ=255)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                     <  2:5052856/101‑1 (MQ=255)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                     <  1:6457391/101‑1 (MQ=255)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                      <  2:5067592/101‑6 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                      >  1:3676504/1‑96 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                    >  2:188824/1‑101 (MQ=255)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                    <  1:5668103/101‑1 (MQ=255)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                    >  1:7076586/1‑101 (MQ=255)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                   >  1:25325/1‑101 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGC.TCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                   <  1:5153679/101‑9 (MQ=2)
                                                                      cccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                <  1:787954/101‑1 (MQ=255)
                                                                       ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                               <  1:416817/101‑1 (MQ=255)
                                                                       ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                               <  1:4831467/101‑1 (MQ=255)
                                                                       ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                               <  2:6668032/101‑1 (MQ=255)
                                                                        cccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                              >  1:2944104/1‑101 (MQ=255)
                                                                          cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                            <  1:901294/101‑1 (MQ=255)
                                                                          cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                            <  1:5023136/101‑1 (MQ=255)
                                                                             gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                         <  2:4562740/101‑1 (MQ=255)
                                                                              ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                        <  1:6559327/101‑1 (MQ=255)
                                                                                     ggATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                 <  1:3599370/101‑1 (MQ=255)
                                                                                         cGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct             <  2:1323700/101‑1 (MQ=255)
                                                                                         cGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct             <  2:2477693/101‑1 (MQ=255)
                                                                                          gAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc            <  1:1786359/101‑1 (MQ=255)
                                                                                          gAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc            <  2:862590/101‑1 (MQ=255)
                                                                                          gAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc            <  2:2324225/101‑1 (MQ=255)
                                                                                           aaaCCGTGGGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa           <  2:648004/101‑1 (MQ=255)
                                                                                             aCCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa         <  1:1288213/101‑1 (MQ=255)
                                                                                               cGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCCGACg       >  1:2236906/1‑101 (MQ=255)
                                                                                               cGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg       <  2:4903310/101‑1 (MQ=255)
                                                                                                gtgtGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt      >  1:6670677/1‑101 (MQ=255)
                                                                                                    ggAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGcc  >  2:2886542/1‑100 (MQ=255)
                                                                                                    |                                                                                                        
CGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGC.GGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCT  >  NC_014034/1396708‑1396908

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG