Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,817 T→G 11.7% H233Q (CAT→CTG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8170T→G11.7% 130.8 ‑2.6 ‑0.5437H233Q (CAT→CAGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/32):  ref base (197/188):  total (216/221)
Fisher's exact test strand distribution p-value = 7.36e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.51e-01

Only 100 of 445 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCG  >  NC_014034/1396717‑1396917
                                                                                                       |                                                                                                    
cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                      <  2:5626280/101‑1 (MQ=255)
  ggggggGGTGGACCAAAGCCCTTTCCCTCATATGCTGCCACGATGCCCTTGAGTAGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGc                                                                                                    <  1:6937150/100‑1 (MQ=37)
   gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCa                                                                                                   <  1:643808/101‑1 (MQ=255)
      ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGGGGAT...GATCATGCAgcg                                                                                                >  1:2735287/1‑101 (MQ=255)
           ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGAc                                                                                           <  1:4634800/101‑1 (MQ=255)
           ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGAc                                                                                           <  1:5442324/101‑1 (MQ=255)
            gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCaagatcggaa                                                                                          >  2:5087159/1‑92 (MQ=255)
            gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                                          >  1:5300604/1‑101 (MQ=255)
             aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACgg                                                                                         >  1:4239129/1‑101 (MQ=255)
                gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                      <  1:1398313/101‑1 (MQ=255)
                gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                      >  2:1314149/1‑101 (MQ=255)
                gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                      >  2:3377179/1‑101 (MQ=255)
                ctaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                      <  1:6347560/99‑1 (MQ=255)
                       tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGc                                                                               >  1:3938185/1‑101 (MQ=255)
                       tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGc                                                                               >  2:3234073/1‑101 (MQ=255)
                        gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTTGAT...GATCATGCAGCGCGGACGGCTTGGGTCACt                                                                              <  2:3045834/101‑1 (MQ=255)
                            cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccc                                                                          >  1:5715313/1‑101 (MQ=255)
                            cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccc                                                                          >  2:310275/1‑101 (MQ=255)
                             tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                         >  2:4358547/1‑101 (MQ=255)
                             tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccccc                                                                         >  2:6977974/1‑101 (MQ=255)
                              cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGCTgcaccc                                                                        >  2:637669/1‑101 (MQ=255)
                               atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                       >  1:5911399/1‑101 (MQ=255)
                                 atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcgcg                                                                     >  2:6812967/1‑101 (MQ=255)
                                  tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgg                                                                    <  1:6547071/101‑1 (MQ=255)
                                  tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgg                                                                    >  1:3434246/1‑101 (MQ=255)
                                     tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCCGCggggg                                                                 >  2:3627971/1‑101 (MQ=255)
                                      gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                                <  2:2809696/101‑1 (MQ=255)
                                      gcCACGATGCCCTTGAGTCGCTCCCCCCGAGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGc                                                                <  1:4506709/101‑1 (MQ=255)
                                        cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGt                                                              <  2:416096/101‑1 (MQ=255)
                                           gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCTGCCGGCTTGGGTCGCGCCCCCCGCGGGGGCGTGGa                                                           <  2:5282494/101‑1 (MQ=255)
                                           gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                           >  1:1232432/1‑101 (MQ=255)
                                             ttgccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:4433743/98‑1 (MQ=255)
                                               catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  1:1521710/94‑1 (MQ=2)
                                                atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  2:2597386/95‑1 (MQ=2)
                                                 cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     >  2:384876/1‑101 (MQ=255)
                                                 cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                     >  1:2879980/1‑101 (MQ=255)
                                                  cgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                      <  1:2999842/97‑1 (MQ=2)
                                                   tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                   >  1:5471299/1‑101 (MQ=255)
                                                   tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                   >  2:4741055/1‑101 (MQ=255)
                                                   tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                   <  2:462101/101‑1 (MQ=255)
                                                   gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     <  2:4858846/98‑1 (MQ=2)
                                                    gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                  >  1:2467255/1‑101 (MQ=255)
                                                    gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                  >  2:2479959/1‑101 (MQ=255)
                                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                   >  1:2663482/2‑100 (MQ=2)
                                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                 >  2:3439251/1‑101 (MQ=255)
                                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                 >  2:1781184/1‑101 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                  <  2:240349/101‑3 (MQ=2)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                 <  1:1003942/101‑4 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                              >  1:898090/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                              <  2:5052856/101‑1 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                              <  2:761027/101‑1 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                              <  2:2139608/101‑1 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                             >  1:6744218/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                             >  1:7076586/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                             <  1:5668103/101‑1 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                             >  2:188824/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                            <  1:6465869/101‑1 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                            >  1:25325/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAGCCGGTcccg                                            >  1:37655/1‑99 (MQ=255)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                             <  1:3186795/101‑8 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                           >  1:2127778/1‑101 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                            <  1:5153679/101‑9 (MQ=2)
                                                             cccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                         <  1:787954/101‑1 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                         <  1:4694083/101‑1 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                                          <  1:1994961/101‑11 (MQ=0)
                                                              ccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatag                                          >  2:4979702/1‑91 (MQ=0)
                                                              ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                        >  2:1606211/1‑101 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                        <  2:6668032/101‑1 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                        >  2:645213/1‑101 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                       <  1:860382/101‑1 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                       <  1:5982386/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                      <  1:3283448/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                      <  2:7075587/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                      <  1:4131442/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                      <  2:3716249/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                     <  1:2396022/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                     <  1:5023136/101‑1 (MQ=255)
                                                                 cgatctggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                     <  2:5370168/95‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                   >  1:179687/1‑101 (MQ=255)
                                                                     ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                 >  2:2696542/1‑101 (MQ=255)
                                                                         cGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                             >  1:1755494/1‑101 (MQ=255)
                                                                         cGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGATTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                             <  1:4878995/101‑1 (MQ=255)
                                                                            ggATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                          <  1:3599370/101‑1 (MQ=255)
                                                                             gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                         <  2:5471027/101‑1 (MQ=255)
                                                                               tCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcc                       <  1:4358769/101‑1 (MQ=255)
                                                                               tCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcc                       >  1:1305638/1‑101 (MQ=255)
                                                                                cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                      >  1:3657111/1‑101 (MQ=255)
                                                                                cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                      <  1:382701/101‑1 (MQ=255)
                                                                                 gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                     <  2:862590/101‑1 (MQ=255)
                                                                                 gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                     <  2:1527837/101‑1 (MQ=255)
                                                                                    aCCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa                  <  1:6090566/101‑1 (MQ=255)
                                                                                      cGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCCGACg                >  1:2236906/1‑101 (MQ=255)
                                                                                       gtgtGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt               <  2:37654/101‑1 (MQ=255)
                                                                                        tgtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc              >  2:6006660/1‑101 (MQ=255)
                                                                                         gtgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg             >  2:6250356/1‑101 (MQ=255)
                                                                                          tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGc            >  2:1014317/1‑101 (MQ=255)
                                                                                           ggAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGcc           >  2:2886542/1‑100 (MQ=255)
                                                                                             at...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcc         >  2:1965392/1‑101 (MQ=255)
                                                                                                    tCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc     >  1:2509593/1‑101 (MQ=255)
                                                                                                     cATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGGTCCAGAGATAGACCGCGCGGGCCTCCTCAGACGTCGCTCCCCCcg    >  2:2260741/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
CGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCG  >  NC_014034/1396717‑1396917

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG