Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,820 A→T 11.9% A234A (GCA→GGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8200A→T11.9% 141.3 ‑3.7 ‑0.0427A234A (GCA→GCT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/33):  ref base (197/179):  total (215/212)
Fisher's exact test strand distribution p-value = 2.51e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.69e-01

Only 100 of 442 total aligned reads displayed.

GGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGG  >  NC_014034/1396720‑1396920
                                                                                                        |                                                                                                    
      gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCgg                                                                                                >  2:4791196/1‑101 (MQ=255)
       tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGa                                                                                               >  1:5780952/1‑101 (MQ=255)
        ggATCGAAACCCTGTCCCTCATAGGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGAc                                                                                              <  1:2762818/101‑1 (MQ=255)
         gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCaagatcggaa                                                                                             >  2:5087159/1‑92 (MQ=255)
          aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                                            >  2:1794341/1‑101 (MQ=255)
          aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                                            >  1:4239129/1‑101 (MQ=255)
            cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCt                                                                                          >  2:7090942/1‑101 (MQ=255)
             gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                         >  2:1314149/1‑101 (MQ=255)
             gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                         >  1:7075961/1‑101 (MQ=255)
             gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                                         >  2:3377179/1‑101 (MQ=255)
              aaaCCCTGTCCCTCATGTGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                                        <  1:1320387/101‑1 (MQ=255)
                  ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                    >  1:2407623/1‑101 (MQ=255)
                  ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                    <  1:6221987/101‑1 (MQ=255)
                  ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                                    >  2:261994/1‑101 (MQ=255)
                   cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGACCCCGGG.CGGAT...GATCATGCAGCGCGGGCGGCTTggggcc                                                                                   >  1:5282760/1‑98 (MQ=37)
                    tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGc                                                                                  >  2:3234073/1‑101 (MQ=255)
                    tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGc                                                                                  >  1:3938185/1‑101 (MQ=255)
                     gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TTGAT...GATCATGCAGCGCGGACGGCTTGGGTCACt                                                                                 <  2:3045834/101‑1 (MQ=255)
                      tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTc                                                                                >  2:5054731/1‑101 (MQ=255)
                       cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGCCGCTcc                                                                               >  1:862614/1‑101 (MQ=255)
                        ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTccc                                                                              <  1:4473970/101‑1 (MQ=255)
                            atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                          <  2:6815936/101‑1 (MQ=255)
                            atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCcg                                                                          >  2:2165290/1‑101 (MQ=255)
                               tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgg                                                                       >  1:3434246/1‑101 (MQ=255)
                                  tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGCTCCCCCCGCggggg                                                                    >  2:3627971/1‑101 (MQ=255)
                                  tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGATTGGGTCGCTCCCCCCGCggggg                                                                    >  2:4878751/1‑101 (MQ=255)
                                     cccGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGt                                                                 <  1:6637403/99‑1 (MQ=255)
                                     cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCgg                                                                 >  2:1904059/1‑100 (MQ=255)
                                     cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGt                                                                 <  2:416096/101‑1 (MQ=255)
                                         aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  1:5426269/1‑101 (MQ=255)
                                         aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  1:595590/1‑101 (MQ=255)
                                          ttgccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  2:4433743/98‑1 (MQ=255)
                                           tcccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCTAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  1:6929961/100‑1 (MQ=255)
                                           acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGGGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  1:5099823/9‑101 (MQ=2)
                                             caggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTC..GACCGAG.TTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          <  2:153389/95‑1 (MQ=2)
                                              cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                        >  1:2879980/1‑101 (MQ=255)
                                               ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  1:1456722/1‑101 (MQ=255)
                                               ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  2:4354710/1‑101 (MQ=255)
                                               cgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  2:7034239/5‑101 (MQ=2)
                                                tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                      <  2:2727612/101‑1 (MQ=255)
                                                tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                      <  2:462101/101‑1 (MQ=255)
                                                 gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                     <  2:6399239/101‑1 (MQ=255)
                                                  cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                      >  1:2663482/2‑100 (MQ=2)
                                                  cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                      <  2:529080/100‑2 (MQ=2)
                                                  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                    >  2:3439251/1‑101 (MQ=255)
                                                  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                    <  1:6058700/101‑1 (MQ=255)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                    <  1:1003942/101‑4 (MQ=2)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                                  >  2:3099625/1‑101 (MQ=255)
                                                    tCGCTCCCCCCGCGGGGGCGCGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGTc                                                  <  1:5600382/101‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                 >  1:898090/1‑101 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                 >  1:4567277/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCag                                               <  1:2541008/101‑1 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAGCCGGTcccg                                               >  1:37655/1‑99 (MQ=255)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                                <  2:6673676/101‑8 (MQ=2)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggcta                                                <  1:3186795/101‑8 (MQ=2)
                                                         ctccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                               <  1:4979954/99‑9 (MQ=0)
                                                         ccccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                               >  1:2468880/1‑93 (MQ=2)
                                                         ccccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                               <  2:3844361/101‑9 (MQ=2)
                                                          cccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                            <  1:4694083/101‑1 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                            <  1:787954/101‑1 (MQ=255)
                                                           ccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                                             <  1:1994961/101‑11 (MQ=0)
                                                           ccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatag                                             >  2:4979702/1‑91 (MQ=0)
                                                           ccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                           >  2:1606211/1‑101 (MQ=255)
                                                           ccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                           <  1:1797126/101‑1 (MQ=255)
                                                           ccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                           <  1:4831467/101‑1 (MQ=255)
                                                           ccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                           <  2:2821359/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                         <  1:4131442/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                         <  2:3716249/101‑1 (MQ=255)
                                                             ccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                         <  1:3283448/101‑1 (MQ=255)
                                                              cgatctggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                        <  2:5370168/95‑1 (MQ=255)
                                                                 gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                     <  1:6398466/101‑1 (MQ=255)
                                                                  gggggttGGAGCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                    <  2:2264618/95‑1 (MQ=255)
                                                                        tGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                              <  2:2498315/101‑1 (MQ=255)
                                                                         ggATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                             <  1:3599370/101‑1 (MQ=255)
                                                                          gATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                            >  1:2130535/1‑101 (MQ=255)
                                                                          gATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                            <  2:5471027/101‑1 (MQ=255)
                                                                            tCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcc                          <  1:4358769/101‑1 (MQ=255)
                                                                              gAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                        <  2:862590/101‑1 (MQ=255)
                                                                               aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                       >  1:1551561/1‑101 (MQ=255)
                                                                               aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                       <  1:5714859/101‑1 (MQ=255)
                                                                               aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                       <  2:2909471/101‑1 (MQ=255)
                                                                                aaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                      >  1:3834197/1‑101 (MQ=255)
                                                                                aaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                      <  2:6316201/101‑1 (MQ=255)
                                                                                aaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTAAg                      <  2:2444980/101‑1 (MQ=255)
                                                                                  ccGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                    >  2:5650854/1‑101 (MQ=255)
                                                                                   cGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCCGACg                   >  1:2236906/1‑101 (MQ=255)
                                                                                   cGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                   >  1:3026428/1‑101 (MQ=255)
                                                                                    gtg.tGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt                  <  2:37654/101‑1 (MQ=255)
                                                                                    gtg.tGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt                  >  1:546958/1‑101 (MQ=255)
                                                                                     tg.tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTc                 >  2:6006660/1‑101 (MQ=255)
                                                                                      g.tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                >  1:1768422/1‑101 (MQ=255)
                                                                                        tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGc               >  2:6517744/1‑101 (MQ=255)
                                                                                        tgGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGc               >  2:1094969/1‑101 (MQ=255)
                                                                                            t...gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc           <  1:3665465/101‑1 (MQ=255)
                                                                                                gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc          >  1:1278064/1‑101 (MQ=255)
                                                                                                gatCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc          >  2:1983325/1‑101 (MQ=255)
                                                                                                 atCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc         <  1:6445957/101‑1 (MQ=255)
                                                                                                 atCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc         >  1:6525948/1‑101 (MQ=255)
                                                                                                     tGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcgcg     >  1:2514915/1‑101 (MQ=255)
                                                                                                        aGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCgggg  >  2:79155/1‑101 (MQ=255)
                                                                                                        |                                                                                                    
GGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGG  >  NC_014034/1396720‑1396920

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG