BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,937	C→A	12.5%	S273R (AGC→AGA)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,937	0	C→A	12.5%	157.8 ‑2.3 ‑0.5	541	S273R (AGC→AGA)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (34/26): ref base (191/235): total (246/295)
Fisher's exact test strand distribution p-value = 9.74e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.86e-01

Only 100 of 562 total aligned reads displayed.


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCG  >  NC_014034/1396837‑1397037


 cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.Gcc                                                                                                                >  1:1130374/1‑101 (MQ=255)


  gCTCCCCCCGCGGGGGCGTGGATCCAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCt                                                                                                               >  2:3402740/1‑101 (MQ=255)


   cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                             <  1:4936181/101‑1 (MQ=255)


   cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                             >  2:573262/1‑101 (MQ=255)


    tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                            <  1:2196078/101‑1 (MQ=255)


    cccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                            <  2:898066/100‑1 (MQ=255)


      cccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcgc                                                                                                          <  2:2010936/101‑1 (MQ=255)


         ccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.Gc                                                                                                      <  2:1792378/101‑1 (MQ=255)


              ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                               >  2:2678799/1‑101 (MQ=255)


               gggCGTTGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                              <  2:5095732/101‑1 (MQ=255)


               gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                              <  2:2474962/101‑1 (MQ=255)


               gatcttGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GA.CGCA.Tc                                                                                              <  1:6575702/96‑1 (MQ=255)


                   gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt                                                                                        >  2:6047217/1‑101 (MQ=255)


                    tGGATCTAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt.t                                                                                      <  2:5943423/101‑1 (MQ=255)


                    tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCt.t                                                                                      <  1:1606272/101‑1 (MQ=255)


                      gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tcc                                                                                    <  2:6844051/101‑1 (MQ=255)


                      gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.Tcc                                                                                    <  2:1652424/101‑1 (MQ=255)


                          gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.Ct                                                                               >  2:5670544/1‑101 (MQ=255)


                           aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTg                                                                              >  2:2114988/1‑101 (MQ=255)


                            aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..c                                                                           <  2:146485/101‑1 (MQ=255)


                              ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..Cgg                                                                         >  2:122390/1‑100 (MQ=255)


                                ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTg.g                                                                      >  1:1651645/1‑101 (MQ=255)


                                 gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.Gt                                                                     <  1:1014352/101‑1 (MQ=255)


                                  tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCGGC.CTG..CGTG.GTc                                                                    >  2:402632/1‑101 (MQ=255)


                                  tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTc                                                                    <  1:310279/101‑1 (MQ=255)


                                          tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                            <  2:2515112/101‑1 (MQ=255)


                                          tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                            <  1:7091316/101‑1 (MQ=255)


                                           aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                           >  2:702831/1‑101 (MQ=255)


                                            gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTccccccc                                                          >  2:1585898/1‑100 (MQ=255)


                                                        ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                               >  1:6745831/11‑101 (MQ=9)


                                                        ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:3141917/91‑1 (MQ=9)


                                                         ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  2:3867606/10‑101 (MQ=2)


                                                          tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:6433906/93‑1 (MQ=2)


                                                          tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  1:1279405/9‑101 (MQ=9)


                                                          gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  2:3677160/94‑1 (MQ=2)


                                                          gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:4768588/94‑1 (MQ=2)


                                                           ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:274083/8‑101 (MQ=2)


                                                           ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  2:3543444/8‑101 (MQ=2)


                                                           caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:5108832/94‑1 (MQ=9)


                                                           caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  1:1464505/8‑101 (MQ=9)


                                                             cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:2017662/97‑1 (MQ=2)


                                                             cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:122391/101‑1 (MQ=255)


                                                             cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         >  1:3175547/1‑101 (MQ=255)


                                                             acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  1:182430/96‑1 (MQ=9)


                                                              aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  1:702851/101‑1 (MQ=255)


                                                               tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  1:3884219/3‑101 (MQ=2)


                                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:3332509/100‑1 (MQ=2)


                                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:5854134/100‑1 (MQ=2)


                                                                cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                      <  1:6963133/99‑2 (MQ=2)


                                                                cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                      >  1:6210111/3‑100 (MQ=2)


                                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  1:6413968/1‑101 (MQ=2)


                                                                 cGTCGCTCCCCCCGCCGGGCGGTGGATCCAAACA.GCC..GCTCG.AC.CGCA.TCGCACCG.GGTCC.CCG..CGCC.GCCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                      <  2:2319531/101‑2 (MQ=2)


                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                    <  2:4965321/101‑4 (MQ=2)


                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                    <  1:1934950/101‑4 (MQ=2)


                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                    <  2:5790450/101‑4 (MQ=2)


                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                     <  2:6528541/101‑3 (MQ=9)


                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                     >  1:2623831/1‑99 (MQ=9)


                                                                   tCGCTCCCCCCGCGGGGGCGTTGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                    <  1:2977699/101‑4 (MQ=2)


                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                   <  2:4592094/101‑1 (MQ=255)


                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                   <  1:222824/101‑1 (MQ=255)


                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                   <  1:2389344/101‑1 (MQ=255)


                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                    >  2:2877016/1‑98 (MQ=9)


                                                                   tCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                   <  1:6047529/101‑1 (MQ=255)


                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                     >  1:4611847/1‑100 (MQ=2)


                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                     >  2:2993651/1‑100 (MQ=2)


                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACTG.GCCCG.GC.CACG.GC...CTG.TGCGC.TGGA.CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                  <  2:2837627/101‑6 (MQ=2)


                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                  >  2:1907558/1‑96 (MQ=2)


                                                                     ccTCCCCCCGCGGGGGCGTGGATCGANNNN.NCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                 <  1:79156/100‑1 (MQ=255)


                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                >  1:553744/1‑94 (MQ=2)


                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccac                                >  2:4673776/1‑94 (MQ=2)


                                                                        ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                              >  2:4139063/1‑92 (MQ=2)


                                                                        ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                               <  1:5270070/101‑9 (MQ=9)


                                                                        ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCG..GATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                               <  1:5759185/101‑9 (MQ=9)


                                                                         cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCA..GGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgt                              <  2:3201685/101‑10 (MQ=2)


                                                                         cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                             <  1:960121/101‑1 (MQ=255)


                                                                         cccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACG..GCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                             <  1:2828917/101‑11 (MQ=2)


                                                                            ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                             <  2:553732/101‑7 (MQ=0)


                                                                            ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                          <  2:2232829/101‑1 (MQ=255)


                                                                             cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCA..TCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                            <  1:3556455/101‑8 (MQ=0)


                                                                             cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                         <  1:4362662/101‑1 (MQ=255)


                                                                              gcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                        <  1:4008226/101‑1 (MQ=255)


                                                                                 ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                     <  1:4194239/101‑1 (MQ=255)


                                                                                 ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGGCGTTgggc                     >  1:6359208/1‑99 (MQ=255)


                                                                                  gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATCAGCTCGGCGTTGGGCa                    >  1:4840583/1‑101 (MQ=255)


                                                                                  gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                    <  2:6670335/101‑1 (MQ=255)


                                                                                  gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                    <  1:340206/101‑1 (MQ=255)


                                                                                  gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                    <  1:566935/101‑1 (MQ=255)


                                                                                     cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                 <  2:1305590/101‑1 (MQ=255)


                                                                                     cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                 <  2:1381927/101‑1 (MQ=255)


                                                                                      gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                >  1:4452202/1‑101 (MQ=255)


                                                                                      gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                >  2:4231118/1‑101 (MQ=255)


                                                                                      gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                >  1:3754556/1‑101 (MQ=255)


                                                                                           gcGAACCC.GCCG.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa           <  2:2156340/100‑1 (MQ=255)


                                                                                             gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg         <  1:5450038/101‑1 (MQ=255)


                                                                                             gAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg         <  2:61725/101‑1 (MQ=255)


                                                                                              aaaCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt        >  2:749848/1‑101 (MQ=255)


                                                                                               aaCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc       <  1:2476756/101‑1 (MQ=255)


                                                                                                aCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGCCGGTGTcc      >  2:1073774/1‑101 (MQ=255)


                                                                                                aCA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc      <  2:3363490/101‑1 (MQ=255)


                                                                                                 cA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg     <  2:5332438/101‑1 (MQ=255)


TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCG..GCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCG  >  NC_014034/1396837‑1397037

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG