Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,959 C→T 32.3% R281C (CGC→TTC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9590C→T32.3% 0.0 ‑2.5 ‑0.1536R281C (CGC→TGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (93/80):  ref base (164/198):  total (257/279)
Fisher's exact test strand distribution p-value = 7.87e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.94e-01

Only 100 of 558 total aligned reads displayed.

GATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1396859‑1397059
                                                                                                           |                                                                                                        
gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.Tcc                                                                                                          <  2:6844051/101‑1 (MQ=255)
   cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGc.c                                                                                                      >  1:136538/1‑101 (MQ=255)
    gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                     <  2:4187990/101‑1 (MQ=255)
    gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                     <  2:1777542/101‑1 (MQ=255)
     aaaCCGGTCCAGAGATATACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTg                                                                                                    >  2:2892474/1‑101 (MQ=255)
           gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.Gt                                                                                           <  1:1014352/101‑1 (MQ=255)
            tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                          <  2:6187633/101‑1 (MQ=255)
            tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                          <  2:6438024/101‑1 (MQ=255)
              cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGGG.GTCGc                                                                                        >  2:1502329/1‑101 (MQ=255)
                   aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                                   >  1:3089302/1‑101 (MQ=255)
                     aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                 <  1:5474537/101‑1 (MQ=255)
                     aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                 <  2:4378986/101‑1 (MQ=255)
                      gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                                                <  1:468519/101‑1 (MQ=255)
                            gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                          <  2:2060205/101‑1 (MQ=255)
                                 ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                      >  1:5778906/11‑101 (MQ=0)
                                 cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGCTCCCCCCGCGGGGGCGTgg                                                                     >  1:5705615/1‑101 (MQ=255)
                                 cctcctCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  2:5715011/101‑1 (MQ=255)
                                  tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  2:1404911/10‑101 (MQ=0)
                                  ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  1:6745831/11‑101 (MQ=9)
                                  ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  1:4864284/11‑101 (MQ=9)
                                   ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   >  2:3867606/10‑101 (MQ=2)
                                   gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  2:5626826/10‑101 (MQ=9)
                                    tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   >  1:1279405/9‑101 (MQ=9)
                                    gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  2:3677160/94‑1 (MQ=2)
                                    cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:3964196/101‑1 (MQ=255)
                                    ccccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:266896/98‑1 (MQ=255)
                                     ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  1:1936314/8‑101 (MQ=2)
                                     ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  2:3543444/8‑101 (MQ=2)
                                     ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  1:274083/8‑101 (MQ=2)
                                     ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  1:7086899/101‑1 (MQ=255)
                                     ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  2:2167637/1‑101 (MQ=255)
                                     cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGG.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  2:2634859/7‑101 (MQ=0)
                                     caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  1:6233245/8‑101 (MQ=9)
                                      ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  1:4790624/6‑101 (MQ=2)
                                      gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                >  1:3750729/7‑101 (MQ=2)
                                      aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  1:6917777/7‑101 (MQ=9)
                                       cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  1:3175547/1‑101 (MQ=255)
                                       acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                <  1:182430/96‑1 (MQ=9)
                                       acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                <  1:2400198/96‑1 (MQ=9)
                                         ccggtccccccccccGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             <  2:2490557/92‑1 (MQ=2)
                                         ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             >  1:2480413/4‑101 (MQ=2)
                                         actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              >  1:4419317/4‑101 (MQ=9)
                                          tcGTCGTTCCCCCCGCGGGTTCCTGATTCTAAACA..TCAG.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..TGTG.GTCGCACCCCCCGCGGGGGCGTGGATCGAAACa                                                            <  2:405880/100‑1 (MQ=21)
                                          ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               <  2:604060/100‑1 (MQ=2)
                                           tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                            <  1:5313027/100‑2 (MQ=9)
                                           tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                            <  2:1630643/100‑2 (MQ=9)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...AGT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                           >  1:5190418/2‑99 (MQ=2)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                           >  2:5479831/2‑99 (MQ=2)
                                            gTCGGTCCACGCGCGGGGGCGTGGAGCGAAACATGGCCA.TCGCC.GC.CGCG.TCGGGC.TTC.C.CAG..CGTC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                           <  2:2284807/101‑3 (MQ=9)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             >  2:3047057/1‑101 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             <  2:213600/101‑1 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                          <  2:5790450/101‑4 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                           <  2:6528541/101‑3 (MQ=9)
                                            gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                             <  1:1169098/101‑1 (MQ=2)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccc                                                          >  2:1022373/1‑98 (MQ=2)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                         <  2:4592094/101‑1 (MQ=255)
                                             ccGCTCCCCCCCGGGGGGCGTGGACCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                         <  2:1098792/100‑1 (MQ=255)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                           >  1:6984598/1‑100 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACTG.GCCCG.GC.CACG.GC.CTG.TGCGC.TGGA.CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                                        <  2:2837627/101‑6 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                       >  1:3389383/1‑101 (MQ=255)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                       <  1:1645540/101‑7 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATGGAAActcggc                                                        >  1:2276543/1‑96 (MQ=9)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                       >  1:6136034/1‑95 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGcc                                                      >  2:1852078/1‑100 (MQ=255)
                                                  ccccccGGGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                     >  1:5982194/1‑93 (MQ=9)
                                                  ccccccGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                       <  2:4688640/101‑3 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                       <  1:3819063/101‑3 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACAT.GGCTAGGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                                                     <  1:4138348/101‑9 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                    >  1:4220069/1‑101 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                    >  2:744687/1‑92 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                    >  2:893597/1‑92 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                     >  2:312568/1‑93 (MQ=9)
                                                   cccccGCGGGGGCGTGGATCGAAACAT.GGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgt                                                    <  2:3201685/101‑10 (MQ=2)
                                                   cccccGCGGGGGCGTGGATCGAAACA...ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                                                   <  1:2828917/101‑11 (MQ=2)
                                                    ccccGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                                     <  2:1984855/101‑5 (MQ=2)
                                                    ccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                   >  2:2440326/1‑91 (MQ=2)
                                                    ccccGCGGGGGCGTGGATCGAAAC...GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                   >  1:6429325/1‑91 (MQ=2)
                                                      ccGCGGGGGCGTGGATCGAAACT..GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                   <  2:7025323/101‑7 (MQ=0)
                                                         cgGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                             <  1:5591954/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                             <  1:2874019/101‑1 (MQ=255)
                                                          ggggcCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                            <  2:5015596/101‑1 (MQ=255)
                                                           ggggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                           <  1:3225231/101‑1 (MQ=255)
                                                            gggCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                          <  1:1585959/101‑1 (MQ=255)
                                                                gTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                      >  1:3522486/1‑101 (MQ=255)
                                                                gTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                      >  2:59166/1‑101 (MQ=255)
                                                                 tGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                     >  1:2205117/1‑101 (MQ=255)
                                                                 tGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                     >  2:1227194/1‑101 (MQ=255)
                                                                  ggATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                    >  1:6361730/1‑101 (MQ=255)
                                                                     tCGAGACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa                                 <  2:3306763/101‑1 (MQ=255)
                                                                        aaaCA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                              <  2:1333742/101‑1 (MQ=255)
                                                                               gCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                         <  2:6672351/101‑1 (MQ=255)
                                                                                       cg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccc                  <  1:2160954/101‑1 (MQ=255)
                                                                                       cg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccc                  >  1:4534156/1‑101 (MQ=255)
                                                                                        g.gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                 <  2:5300338/101‑1 (MQ=255)
                                                                                        g.gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                 >  1:2032115/1‑101 (MQ=255)
                                                                                        g.gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCCTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                 >  2:3451264/1‑101 (MQ=255)
                                                                                              gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg             >  2:3976264/1‑101 (MQ=255)
                                                                                              gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg             >  1:5025948/1‑101 (MQ=255)
                                                                                                    ggCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc        >  1:868398/1‑101 (MQ=255)
                                                                                                       t.tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTg     <  2:4946475/101‑1 (MQ=255)
                                                                                                           |                                                                                                        
GATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1396859‑1397059

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG