Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,960 G→T 10.2% R281L (CGC→TTC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9600G→T10.2% 129.0 ‑1.9 ‑0.0535R281L (CGC→CTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/34):  ref base (232/245):  total (255/280)
Fisher's exact test strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.89e-01

Only 100 of 562 total aligned reads displayed.

ATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396860‑1397060
                                                                                                           |                                                                                                        
  cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGc.c                                                                                                       <  2:3938010/101‑1 (MQ=255)
   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGc.cc                                                                                                      >  2:3225115/1‑100 (MQ=255)
   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAANNN.NNCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.Ct                                                                                                      >  1:4253190/1‑101 (MQ=255)
   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGc.cc                                                                                                      >  2:684217/1‑100 (MQ=255)
   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGc.cc                                                                                                      >  2:6091773/1‑100 (MQ=255)
   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.Ct                                                                                                      <  2:179684/101‑1 (MQ=255)
    aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTg                                                                                                     <  1:2678895/101‑1 (MQ=255)
    aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTg                                                                                                     >  2:2114988/1‑101 (MQ=255)
       ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGt                                                                                                <  1:5466162/101‑1 (MQ=255)
          gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.Gt                                                                                            <  1:1014352/101‑1 (MQ=255)
           tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                           <  2:6438024/101‑1 (MQ=255)
            ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCg                                                                                          >  2:4008051/1‑101 (MQ=255)
               gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTc                                                                                       >  1:5671499/1‑101 (MQ=255)
                  aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                                    <  2:1337654/101‑1 (MQ=255)
                   tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                                   <  1:7091316/101‑1 (MQ=255)
                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                  >  2:2476662/1‑101 (MQ=255)
                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                  >  1:3136302/1‑101 (MQ=255)
                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                                  <  1:891177/101‑1 (MQ=255)
                        cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCTCgg                                                                              <  2:1456662/101‑1 (MQ=255)
                            cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                          <  1:3417197/101‑1 (MQ=255)
                                ctcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                       <  1:3973994/91‑1 (MQ=0)
                                 ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                      >  1:6745831/11‑101 (MQ=9)
                                 attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:4989459/11‑101 (MQ=0)
                                 attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:335455/11‑101 (MQ=0)
                                   tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:1244526/93‑1 (MQ=2)
                                   tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  2:3516712/9‑101 (MQ=2)
                                   tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    >  1:1279405/9‑101 (MQ=9)
                                   gcggttgcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  2:2210960/94‑1 (MQ=2)
                                    ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  2:3616578/94‑1 (MQ=2)
                                    cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  2:5954474/95‑1 (MQ=2)
                                    caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:1101817/94‑1 (MQ=9)
                                    caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  1:1464505/8‑101 (MQ=9)
                                    caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:5108832/94‑1 (MQ=9)
                                     aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  1:1954229/7‑101 (MQ=9)
                                      cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  1:4741295/101‑1 (MQ=255)
                                      acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 >  2:1518718/6‑101 (MQ=9)
                                       ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  2:6623595/98‑1 (MQ=2)
                                       aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               >  2:2398026/1‑101 (MQ=255)
                                       aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               <  2:2154608/101‑1 (MQ=255)
                                        tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               >  1:3884219/3‑101 (MQ=2)
                                         ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  2:3368841/3‑101 (MQ=9)
                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             <  2:4640997/99‑2 (MQ=2)
                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             >  1:6210111/3‑100 (MQ=2)
                                         ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  1:1599949/100‑1 (MQ=2)
                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                            >  2:1643646/2‑99 (MQ=2)
                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               >  1:6413968/1‑101 (MQ=2)
                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            >  2:2687303/1‑100 (MQ=255)
                                          cGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                            <  1:592315/101‑1 (MQ=255)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                                           <  2:4965321/101‑4 (MQ=2)
                                           gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              <  1:1169098/101‑1 (MQ=2)
                                           gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                            <  1:2852264/101‑3 (MQ=2)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                          <  1:222824/101‑1 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCCCTCCCCCCGCGGGGGCGTGGGTCCCAACaccc                                                          >  2:5744920/1‑98 (MQ=37)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                           >  2:1037758/1‑98 (MQ=9)
                                            ccGCTCCCCCCGGCGGGGCGTGGATCGAAACA.CCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                          <  2:2533431/100‑1 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            >  1:4611847/1‑100 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                            <  1:5279216/101‑2 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                           >  1:7000007/1‑99 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                         >  2:1907558/1‑96 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                         >  2:6216243/1‑96 (MQ=9)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                         >  2:3843686/1‑96 (MQ=9)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                       >  1:1683084/1‑94 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaagatcgga                                                       >  1:3202270/1‑93 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccgccac                                                       >  1:2496554/1‑94 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CC.AGGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                        <  2:3173614/101‑3 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                     >  2:893597/1‑92 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA..ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                     >  2:744687/1‑92 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG..CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                      <  1:5759185/101‑9 (MQ=9)
                                                 ccccccGCGGGGGCGGGGATCGAAACATGGCTATGGCG.GC.AGTG..TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                                                      <  2:1994351/101‑9 (MQ=2)
                                                    cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                     <  2:287294/101‑6 (MQ=0)
                                                     ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                    <  2:553732/101‑7 (MQ=0)
                                                     ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                    <  2:7025323/101‑7 (MQ=0)
                                                     ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAAT.TCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                    <  1:244059/101‑7 (MQ=0)
                                                          ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                            <  1:4194239/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTc                                            >  1:6092203/1‑101 (MQ=255)
                                                          ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGGCGTTgggc                                            >  1:6359208/1‑99 (MQ=255)
                                                           gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATCAGCTCGGCGTTGGGCa                                           >  1:4840583/1‑101 (MQ=255)
                                                               gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                       >  1:4452202/1‑101 (MQ=255)
                                                                tGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                      >  2:5227036/1‑101 (MQ=255)
                                                                 ggATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     >  2:2983762/1‑101 (MQ=255)
                                                                 ggATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     >  1:3051146/1‑101 (MQ=255)
                                                                   aTCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                                   <  1:2167717/101‑1 (MQ=255)
                                                                     cGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                 >  2:4089102/1‑101 (MQ=255)
                                                                     cGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                 >  1:3796274/1‑101 (MQ=255)
                                                                          cA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                            <  2:5332438/101‑1 (MQ=255)
                                                                          cA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                            <  2:3056437/101‑1 (MQ=255)
                                                                                      g.gC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc                  <  2:5839984/101‑1 (MQ=255)
                                                                                           cGCA..TCGGCT.TCCGT.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgc               >  1:5573282/1‑101 (MQ=255)
                                                                                            gCA..TCGGCG.TCCGG.CTG..CGTG.GTCCGCCCCCCCGCGGGGGCGTGGAGCGAAACCTCCGCCCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg              <  1:2295269/101‑1 (MQ=37)
                                                                                              a..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg            >  2:4707696/1‑101 (MQ=255)
                                                                                              a..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg            >  1:1838578/1‑101 (MQ=255)
                                                                                                 tCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg           >  2:5662511/1‑101 (MQ=255)
                                                                                                 tCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg           >  1:2782708/1‑101 (MQ=255)
                                                                                                 tCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg           >  1:2078322/1‑101 (MQ=255)
                                                                                                    gCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg        >  1:695906/1‑101 (MQ=255)
                                                                                                     cT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCgg       >  2:4409490/1‑100 (MQ=255)
                                                                                                           gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc  <  1:6518074/101‑1 (MQ=255)
                                                                                                           gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc  <  2:6525617/101‑1 (MQ=255)
                                                                                                           gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc  <  2:4219874/101‑1 (MQ=255)
                                                                                                           gC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc  <  1:3621509/101‑1 (MQ=255)
                                                                                                           |                                                                                                        
ATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA..TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396860‑1397060

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG