Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,002 T→C 12.0% I295T (ATC→ACC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0020T→C12.0% 127.1 ‑1.4 ‑1.3459I295T (ATC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (31/24):  ref base (192/209):  total (223/236)
Fisher's exact test strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.50e-02

Only 100 of 476 total aligned reads displayed.

CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTC  >  NC_014034/1396902‑1397102
                                                                                                       |                                                                                                         
cGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                                                           <  1:592315/101‑1 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                                                                         >  1:61726/1‑101 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                                                                         <  1:23042/101‑1 (MQ=255)
  cccgcccccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                                                                         <  2:533246/96‑1 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                                                                       >  1:2853839/1‑101 (MQ=255)
    gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                                                                       <  1:2640560/101‑1 (MQ=255)
      cccccccGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                                                                                        <  2:6673804/100‑2 (MQ=2)
       ccccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                                                                    >  1:4220069/1‑101 (MQ=255)
         ccccGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaagat                                                                                                     >  1:7083844/1‑97 (MQ=2)
         ccccGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.tc                                                                                                 <  1:2153006/101‑1 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                                                                    <  1:2098123/101‑6 (MQ=0)
           ccGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                                                                   <  2:7025323/101‑7 (MQ=0)
            cgcgGGGGCGTGGATCGAAACTGCGTGCGCGCCATGAATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                                                                                  <  1:3556455/101‑8 (MQ=0)
             gcgGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTg                                                                                             <  1:4008226/101‑1 (MQ=255)
               gggggCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTggc                                                                                           >  1:3208237/1‑100 (MQ=255)
                 gggCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCa                                                                                         <  1:340206/101‑1 (MQ=255)
                    cGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                                                      <  2:1381927/101‑1 (MQ=255)
                    cGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                                                      <  1:52113/101‑1 (MQ=255)
                     gTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCg                                                                                     >  2:59166/1‑101 (MQ=255)
                       ggATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCggc                                                                                   >  2:1040174/1‑100 (MQ=255)
                       ggATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGa                                                                                   >  1:6361730/1‑101 (MQ=255)
                           cGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GAt                                                                              >  1:3796274/1‑101 (MQ=255)
                            gAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATg                                                                             >  2:3196111/1‑101 (MQ=255)
                            gAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATg                                                                             <  2:61725/101‑1 (MQ=255)
                            gAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATg                                                                             >  2:1512950/1‑101 (MQ=255)
                             aaaCAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGt                                                                            <  2:1333742/101‑1 (MQ=255)
                             aaaCAGCCTGCGCGGC.CGCA.TCGGCTTCCCCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAACCATCAGCTCG.GCGTTGGTCAGCCCGAC.GATGt                                                                            >  2:5800637/1‑101 (MQ=38)
                              aaCAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                           <  1:2476756/101‑1 (MQ=255)
                                 aGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTAGTCAGCCGGAC.GATGTCC...Gt                                                                     >  1:5631395/1‑101 (MQ=255)
                                    cTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGc                                                                  >  2:3561792/1‑101 (MQ=255)
                                        gcgGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccc                                                              >  2:2879748/1‑101 (MQ=255)
                                         gccgggcccgtCGGCTTCCGGCTTTGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcc                                                               <  2:655325/91‑1 (MQ=37)
                                          ggC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccccc                                                            >  1:2032115/1‑101 (MQ=255)
                                          ggC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCC.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccccc                                                            >  2:3451264/1‑101 (MQ=255)
                                           gC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccccc                                                           >  2:487106/1‑101 (MQ=255)
                                               gCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                        >  2:3976264/1‑101 (MQ=255)
                                               gCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                        >  1:5025948/1‑101 (MQ=255)
                                                 a.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggg                                                      >  1:95926/1‑101 (MQ=255)
                                                 a.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggg                                                      >  2:4707696/1‑101 (MQ=255)
                                                   tCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCgggg                                                     >  2:5662511/1‑101 (MQ=255)
                                                        ttCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                <  2:4946475/101‑1 (MQ=255)
                                                          ccGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCGGCCGGAC.GATGTCC...GTCGCGCCCCCCGCGGGGGCGTGGa                                              >  2:1894539/1‑101 (MQ=255)
                                                            gCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGaga                                            >  2:6380102/1‑99 (MQ=255)
                                                            gCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:4219874/101‑1 (MQ=255)
                                                             ccTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:5467930/101‑1 (MQ=255)
                                                             ccTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:1600350/101‑1 (MQ=255)
                                                              ccagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  1:4983926/9‑101 (MQ=2)
                                                               ccgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:3918233/96‑1 (MQ=2)
                                                               ccgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:5214004/96‑1 (MQ=2)
                                                                agcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           >  1:220514/7‑101 (MQ=2)
                                                                 gcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  2:2081279/6‑101 (MQ=2)
                                                                  gTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                      >  1:1003564/1‑101 (MQ=255)
                                                                  ccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        <  2:314781/99‑1 (MQ=2)
                                                                   ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:2506598/4‑101 (MQ=0)
                                                                    g.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                    >  2:2548477/1‑101 (MQ=255)
                                                                       tCGCTCCCCCCGCGGGGGCGTGGATCG.AACCCCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaac                                    <  2:5554281/101‑4 (MQ=2)
                                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcg                                   >  1:3658747/1‑100 (MQ=2)
                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                  >  1:6661800/1‑99 (MQ=2)
                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                  >  2:2007162/1‑96 (MQ=2)
                                                                          cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactga                                 >  2:1825672/1‑95 (MQ=2)
                                                                           tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                              >  1:171485/1‑101 (MQ=255)
                                                                           cccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                              <  2:4086240/100‑1 (MQ=255)
                                                                            ccccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                               <  2:1126596/101‑6 (MQ=2)
                                                                            ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                             >  1:115164/1‑101 (MQ=255)
                                                                             cccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagat                              <  2:813925/101‑7 (MQ=2)
                                                                             cccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                            <  1:4527446/101‑1 (MQ=255)
                                                                             cccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                            <  2:3745615/101‑1 (MQ=255)
                                                                             cccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGa                            <  2:6827615/101‑1 (MQ=255)
                                                                              ccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatc                             >  2:3080597/1‑94 (MQ=2)
                                                                              ccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                           >  1:4592448/1‑101 (MQ=255)
                                                                                ccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGcgcagctcgg                           >  2:6266993/1‑92 (MQ=0)
                                                                                ccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGGGGGGACCGAAACTGCCGCCTTgggga                         >  1:3074505/1‑97 (MQ=38)
                                                                                ccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTg                         >  2:4420693/1‑101 (MQ=255)
                                                                                 cgcgGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatctcg                          <  1:3338831/101‑11 (MQ=0)
                                                                                  gcgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTcgagaaggcc                       <  2:2200115/101‑10 (MQ=255)
                                                                                  gcgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGAt                       >  1:1415791/1‑101 (MQ=255)
                                                                                  gcgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGAt                       >  2:1602819/1‑101 (MQ=255)
                                                                                   cgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATg                      >  1:4833017/1‑101 (MQ=255)
                                                                                   cgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATg                      <  1:4688869/101‑1 (MQ=255)
                                                                                    gggggCTTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                     >  1:2983756/1‑101 (MQ=255)
                                                                                    gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                     >  2:2737281/1‑101 (MQ=255)
                                                                                     ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                    >  2:6070840/1‑101 (MQ=255)
                                                                                     ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                    >  1:6950634/1‑101 (MQ=255)
                                                                                        gCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgggggg                 >  1:6599498/1‑99 (MQ=255)
                                                                                          gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg               <  1:6508498/101‑1 (MQ=255)
                                                                                           ttgATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa              >  1:6815291/3‑101 (MQ=255)
                                                                                            ggATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg             >  2:4288078/1‑101 (MQ=255)
                                                                                             gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc            <  2:2398490/101‑1 (MQ=255)
                                                                                              aTCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGca           >  1:813880/1‑101 (MQ=255)
                                                                                               gcGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcac          <  2:1178447/100‑1 (MQ=255)
                                                                                                cGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCTCGAggaga         >  2:4538158/1‑97 (MQ=255)
                                                                                                cGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcaca         >  1:53374/1‑101 (MQ=255)
                                                                                                 gANANNTCAGCTCG.TCGTTGGTCAGNNNNNC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt        <  1:1327593/101‑1 (MQ=255)
                                                                                                  aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg       <  1:577418/101‑1 (MQ=255)
                                                                                                   aaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt      <  1:384880/101‑1 (MQ=255)
                                                                                                   aaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt      <  1:4421518/101‑1 (MQ=255)
                                                                                                    aCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc     >  2:3846939/1‑101 (MQ=255)
                                                                                                    aCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc     >  2:1685914/1‑101 (MQ=255)
                                                                                                     cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg    >  1:2723001/1‑101 (MQ=255)
                                                                                                     cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg    <  1:1842542/101‑1 (MQ=255)
                                                                                                       |                                                                                                         
CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGC.CGCA.TCGGCTTCCGCCTGCGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTC  >  NC_014034/1396902‑1397102

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG