Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,066 T→G 4.5% T316T (ACT→ACG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0660T→G 4.5% 28.9 ‑0.2 ‑0.7395T316T (ACT→ACGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/9):  ref base (202/175):  total (211/184)
Fisher's exact test strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.78e-01

Only 100 of 409 total aligned reads displayed.

GTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCC  >  NC_014034/1396966‑1397166
                                                                                                      |                                                                                                    
gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                      >  1:1003564/1‑101 (MQ=255)
gTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                      >  2:1472532/1‑101 (MQ=255)
  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                    <  1:741681/101‑1 (MQ=255)
   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcc                                                                                                   <  2:6241030/101‑1 (MQ=255)
    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCg                                                                                                  >  2:2050378/1‑101 (MQ=255)
    tCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCg                                                                                                  <  1:5696845/101‑1 (MQ=255)
     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                                                                                 <  2:5412381/101‑1 (MQ=255)
     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                                                                                 >  1:2249594/1‑101 (MQ=255)
      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                                                                                >  1:5797554/1‑101 (MQ=255)
       cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgta                                                                                                 <  2:2570637/101‑4 (MQ=2)
        cccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                                                                              <  2:4086240/100‑1 (MQ=255)
         ccccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                                                                               >  1:1396102/1‑96 (MQ=2)
         ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                                                                             >  1:115164/1‑101 (MQ=255)
         ccccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                                                                             >  1:3380956/1‑101 (MQ=255)
           ccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatc                                                                                             <  2:4229538/101‑8 (MQ=2)
           ccccGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                                                                                           >  1:3111618/1‑101 (MQ=255)
               gcgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGAt                                                                                       >  2:1602819/1‑101 (MQ=255)
                cgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATg                                                                                      >  1:4833017/1‑101 (MQ=255)
                cgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATg                                                                                      <  1:4688869/101‑1 (MQ=255)
                 gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                                                                     >  1:847432/1‑101 (MQ=255)
                 gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                                                                     >  2:2191056/1‑101 (MQ=255)
                  ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                                                                                    >  1:6581559/1‑101 (MQ=255)
                   gggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgggg                                                                                   <  2:4314086/101‑1 (MQ=255)
                   gggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGCCGTGGATCGAAACTGCCGTCTTGAGTGATgggg                                                                                   <  1:5004165/101‑1 (MQ=255)
                       gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                                                                               >  2:3126400/1‑101 (MQ=255)
                        ttgATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                                                                              >  1:6815291/3‑101 (MQ=255)
                         ggATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                                                                             >  1:2105736/1‑101 (MQ=255)
                          gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                                                            <  2:1145054/101‑1 (MQ=255)
                          gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                                                            <  2:2398490/101‑1 (MQ=255)
                          gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                                                            <  2:6688730/101‑1 (MQ=255)
                          gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                                                            >  1:2538828/1‑101 (MQ=255)
                              gANANNTCAGCTCG.TCGTTGGTCAGNNNNNC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                                                        <  1:1327593/101‑1 (MQ=255)
                              gAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                                                        >  1:2706576/1‑101 (MQ=255)
                               aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                                                                       >  1:453985/1‑101 (MQ=255)
                                aaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                                                                      >  2:3467899/1‑101 (MQ=255)
                                 aCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc                                                                     >  2:1685914/1‑101 (MQ=255)
                                 aCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc                                                                     <  1:1644476/101‑1 (MQ=255)
                                  cATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcg                                                                    <  1:1842542/101‑1 (MQ=255)
                                    tCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc                                                                  >  2:3906263/1‑101 (MQ=255)
                                    tCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgtc                                                                  <  2:2509499/101‑1 (MQ=255)
                                     cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg                                                                 >  1:5228576/1‑101 (MQ=255)
                                     cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg                                                                 >  2:1192795/1‑101 (MQ=255)
                                     cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGtcgtcg                                                                 >  2:5227592/1‑101 (MQ=255)
                                         tcg.tcgTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcc                                                             <  2:6814937/101‑1 (MQ=255)
                                          cg.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCCCATGTCGTCGCTccc                                                            >  2:3540380/1‑101 (MQ=255)
                                          cg.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc                                                            <  1:1713554/101‑1 (MQ=255)
                                           g.tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccc                                                           >  1:2927544/1‑101 (MQ=255)
                                             tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc                                                          <  2:3051033/101‑1 (MQ=255)
                                             tcgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc                                                          >  1:4342633/1‑101 (MQ=255)
                                              cgtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                                                         <  2:5055507/101‑1 (MQ=255)
                                               gtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcg                                                        >  1:4211200/1‑101 (MQ=255)
                                               gtTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcg                                                        >  1:1483929/1‑101 (MQ=255)
                                                   gTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCggg                                                    >  2:3655987/1‑101 (MQ=255)
                                                    tCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGGTCTTGAGGGAGGGGGCGCGAGCAAATGCCGTAGCTCCCCCCGCgagg                                                   >  2:7026267/1‑101 (MQ=25)
                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  1:2200194/1‑101 (MQ=255)
                                                           gAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGa                                            >  2:3184894/1‑101 (MQ=255)
                                                             c.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                          <  1:5914753/101‑1 (MQ=255)
                                                             c.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGGGGATc                                          >  2:4526088/1‑101 (MQ=255)
                                                               gATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:330688/1‑101 (MQ=255)
                                                               gATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:660706/1‑101 (MQ=255)
                                                               gATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:5160372/1‑101 (MQ=255)
                                                               gATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:638817/1‑101 (MQ=255)
                                                                aTGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  2:6167227/1‑101 (MQ=255)
                                                                 tGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                       <  1:2050458/101‑1 (MQ=255)
                                                                    ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                    <  2:5755316/101‑1 (MQ=255)
                                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                  >  2:5873743/1‑101 (MQ=255)
                                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                  >  2:3080392/1‑101 (MQ=255)
                                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                  >  1:6311954/1‑101 (MQ=255)
                                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                <  2:6361406/101‑1 (MQ=255)
                                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                >  1:2838020/1‑101 (MQ=255)
                                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                >  2:4557648/1‑101 (MQ=255)
                                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAg                               <  2:7123612/101‑1 (MQ=255)
                                                                           cccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                             <  1:3446425/100‑1 (MQ=255)
                                                                           cccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                             <  2:330684/100‑1 (MQ=255)
                                                                             cccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcg                           <  1:3857829/101‑1 (MQ=255)
                                                                                   cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                     <  1:3628120/101‑1 (MQ=255)
                                                                                   cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                     <  1:6092085/101‑1 (MQ=255)
                                                                                   cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                     >  1:5962092/1‑101 (MQ=255)
                                                                                   cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                     >  2:2701541/1‑101 (MQ=255)
                                                                                   cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                     >  1:5753042/1‑101 (MQ=255)
                                                                                    gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                    >  1:6909723/1‑101 (MQ=255)
                                                                                      gggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATg                  <  2:6599158/101‑1 (MQ=255)
                                                                                      gggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATg                  >  1:6667346/1‑101 (MQ=255)
                                                                                       ggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGt                 >  1:4063022/1‑101 (MQ=255)
                                                                                          gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt              >  1:6990202/1‑101 (MQ=255)
                                                                                           tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc             >  2:5503271/1‑101 (MQ=255)
                                                                                           tGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTc             >  2:2204460/1‑101 (MQ=255)
                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTca            >  1:1785157/1‑100 (MQ=255)
                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg            <  2:483293/101‑1 (MQ=255)
                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg            >  2:4675849/1‑101 (MQ=255)
                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg            >  1:4767399/1‑101 (MQ=255)
                                                                                            ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg            >  1:4438163/1‑101 (MQ=255)
                                                                                             gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa           <  1:3723800/101‑1 (MQ=255)
                                                                                              aTCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc          <  2:813856/101‑1 (MQ=255)
                                                                                              aTCGAAACTGCCGTCTTGAGCGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc          <  1:5600919/101‑1 (MQ=255)
                                                                                               tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc         <  2:4431108/101‑1 (MQ=255)
                                                                                               tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc         <  2:3801321/101‑1 (MQ=255)
                                                                                                cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCg        <  1:1244632/101‑1 (MQ=255)
                                                                                                   aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTgc     >  2:1805233/1‑101 (MQ=255)
                                                                                                     cTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTgcgc   >  2:1017219/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCC  >  NC_014034/1396966‑1397166

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG