Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,132 G→A 6.4% T338T (ACG→ACA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1320G→A 6.4% 55.4 ‑1.2 ‑1.9375T338T (ACG→ACARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/15):  ref base (176/175):  total (185/190)
Fisher's exact test strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.38e-02

Only 100 of 397 total aligned reads displayed.

CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGC  >  NC_014034/1397032‑1397232
                                                                                                    |                                                                                                     
ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                                                       <  2:5755316/101‑1 (MQ=255)
  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTc                                                                                                     >  1:1415241/1‑101 (MQ=255)
   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTcc                                                                                                    >  1:6312439/1‑101 (MQ=255)
   tCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTcc                                                                                                    <  1:2278994/101‑1 (MQ=255)
    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                   <  1:1685975/101‑1 (MQ=255)
    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCa                                                                                                   <  2:2696413/101‑1 (MQ=255)
       tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGcc                                                                                                >  2:3612237/1‑100 (MQ=255)
       tCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                                                                                                <  1:4505287/101‑1 (MQ=255)
       cccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                                                                                                <  2:5797252/100‑1 (MQ=255)
       cccccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCt                                                                                                <  2:330684/100‑1 (MQ=255)
         cccccGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTcg                                                                                              <  2:1551501/101‑1 (MQ=255)
              gcgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCca                                                                                         <  1:59167/101‑1 (MQ=255)
               ctggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCATATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCCGCTCGCGCcac                                                                                        >  2:6150898/3‑101 (MQ=255)
               cgGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcac                                                                                        <  1:6092085/101‑1 (MQ=255)
                gggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCcaca                                                                                       >  2:6614634/1‑101 (MQ=255)
                 ggggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACAt                                                                                      <  1:687572/101‑1 (MQ=255)
                   ggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGt                                                                                    <  2:2243755/101‑1 (MQ=255)
                   ggCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGt                                                                                    >  2:4541883/1‑101 (MQ=255)
                     cGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGTCAAGCTCGCGCCACATGGCt                                                                                  >  2:5087776/1‑101 (MQ=255)
                      gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCtt                                                                                 >  2:808841/1‑101 (MQ=255)
                      gTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACGTCCAGCTCGGCCCACATGGCtt                                                                                 >  2:3144855/1‑101 (MQ=38)
                        ggATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCg                                                                               >  2:3784646/1‑101 (MQ=255)
                         gATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGa                                                                              >  1:7061108/1‑101 (MQ=255)
                          aTCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc                                                                             >  1:3376185/1‑101 (MQ=255)
                          aTCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc                                                                             <  2:3745350/101‑1 (MQ=255)
                          aTCGAAACTGCCGTCTTGAGCGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAc                                                                             <  1:5600919/101‑1 (MQ=255)
                           tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                            <  2:4431108/101‑1 (MQ=255)
                           tCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGAcc                                                                            <  1:5227857/101‑1 (MQ=255)
                            cGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.g                                                                          <  2:1159768/101‑1 (MQ=255)
                             gAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.Gt                                                                         <  2:3226044/101‑1 (MQ=255)
                               aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                       >  2:2734358/1‑101 (MQ=255)
                                   gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                   <  2:5572992/101‑1 (MQ=255)
                                   gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                   >  2:6979428/1‑101 (MQ=255)
                                    ccGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGt                                                                  <  1:2074896/101‑1 (MQ=255)
                                      gTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCg                                                                >  1:1553942/1‑101 (MQ=255)
                                        cTTGAGTGGTGGGGCGCGAGCACGTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCCCATGTCTTCGCCC.GTGCGCCGTCGCt                                                              <  1:6969748/101‑1 (MQ=38)
                                        cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCt                                                              >  1:6782371/1‑101 (MQ=255)
                                        cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCt                                                              >  2:1316454/1‑101 (MQ=255)
                                         ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                             >  2:2618645/1‑101 (MQ=255)
                                         ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                             <  2:5214657/101‑1 (MQ=255)
                                          tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                            <  1:3080505/101‑1 (MQ=255)
                                           gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                           >  2:1472115/1‑101 (MQ=255)
                                                aTGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCGCcgc                                                      >  2:4646126/1‑101 (MQ=255)
                                                aTGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgc                                                      <  2:4740840/101‑1 (MQ=255)
                                                  ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                    >  1:547659/1‑101 (MQ=255)
                                                   gggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCggg                                                   <  1:2202420/101‑1 (MQ=255)
                                                        cgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGt                                              <  2:4832774/101‑1 (MQ=255)
                                                         gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                             <  2:1003529/101‑1 (MQ=255)
                                                            cggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  1:3146563/93‑1 (MQ=0)
                                                            cccaTGTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  2:4216790/99‑1 (MQ=255)
                                                            cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                          <  1:3906438/101‑1 (MQ=255)
                                                             ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  2:6660189/8‑101 (MQ=0)
                                                             ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  1:1025242/94‑1 (MQ=0)
                                                             ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  1:4569868/94‑1 (MQ=0)
                                                             ggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  1:4980187/94‑1 (MQ=0)
                                                             acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  1:6700062/1‑101 (MQ=255)
                                                             acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  2:200969/1‑101 (MQ=255)
                                                             acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GGGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                         >  1:5772888/1‑101 (MQ=255)
                                                              caTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                        <  2:3074392/101‑1 (MQ=255)
                                                                tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                      >  1:3509427/5‑101 (MQ=2)
                                                                tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                      <  1:2045557/97‑1 (MQ=2)
                                                                 gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGCTCGaaa                                     >  1:1308119/1‑101 (MQ=255)
                                                                 gtcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                     >  1:3810230/1‑101 (MQ=255)
                                                                    gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgt                                  <  2:171482/101‑1 (MQ=255)
                                                                      cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                >  2:682306/1‑101 (MQ=255)
                                                                        ccccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                              <  2:6286173/99‑1 (MQ=255)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                              <  1:4875801/101‑1 (MQ=255)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                              >  1:5688271/1‑101 (MQ=255)
                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                              <  1:121639/101‑1 (MQ=255)
                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                             >  2:5371118/1‑101 (MQ=255)
                                                                           cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                           <  1:4241269/101‑1 (MQ=255)
                                                                           cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                           <  1:4039041/101‑1 (MQ=255)
                                                                           cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                           <  2:5671209/101‑1 (MQ=255)
                                                                           cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCACCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGCCTGcc                           >  1:2801455/1‑101 (MQ=255)
                                                                             cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCac                         <  2:749621/101‑1 (MQ=255)
                                                                               cgcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacga                       >  2:5898752/1‑101 (MQ=255)
                                                                                gcgGGGGCGTTGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                      <  1:4646365/101‑1 (MQ=255)
                                                                                gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgac                      <  1:6236879/101‑1 (MQ=255)
                                                                                 cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                     <  2:6382712/101‑1 (MQ=255)
                                                                                 cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCacgacg                     <  2:4989199/101‑1 (MQ=255)
                                                                                      gCGTGGATCGAAACGTCCAACTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTg                >  1:344888/1‑101 (MQ=255)
                                                                                       cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGc               >  1:3637478/1‑101 (MQ=255)
                                                                                       cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGc               <  2:4062846/101‑1 (MQ=255)
                                                                                        gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCa              <  1:4089296/101‑1 (MQ=255)
                                                                                        gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCAGCa              >  1:6525894/1‑101 (MQ=255)
                                                                                         tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAc             <  1:1819846/101‑1 (MQ=255)
                                                                                          ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCAcc            >  1:4407867/1‑101 (MQ=255)
                                                                                             tCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGa         >  2:864964/1‑101 (MQ=255)
                                                                                              cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGAt        >  2:2067321/1‑101 (MQ=255)
                                                                                               gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc       <  1:2680986/101‑1 (MQ=255)
                                                                                               gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc       >  1:4802261/1‑101 (MQ=255)
                                                                                               gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATc       <  2:2200099/101‑1 (MQ=255)
                                                                                                aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc      >  2:6564387/1‑101 (MQ=255)
                                                                                                aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc      >  2:3385896/1‑101 (MQ=255)
                                                                                                aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc      >  1:5579358/1‑101 (MQ=255)
                                                                                                aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATcc      >  1:804848/1‑101 (MQ=255)
                                                                                                   cGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCTCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgcg   >  2:4865325/1‑101 (MQ=255)
                                                                                                   cGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCcgcg   <  2:1836084/101‑1 (MQ=255)
                                                                                                    gTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCgcgc  >  1:2967859/1‑101 (MQ=255)
                                                                                                    gTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCgcgc  >  2:6738186/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGC  >  NC_014034/1397032‑1397232

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG